Plant Communications最新文献_第4页

The NLP-HSF regulatory module contributes to nitrogen-mediated thermotolerance in rice. NLP-HSF调控模块有助于水稻氮介导的耐热性。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 DOI: 10.1016/j.xplc.2025.101522

Dong-Jie Zhu, Zi-Sheng Zhang, Tao Qing, Juan Gao, Cheng-Bin Xiang, Jian-Xiang Liu

引用次数: 0

DeepPGDB: A Novel Paradigm for AI-Guided Interactive Plant Genomic Database. DeepPGDB：人工智能引导的交互式植物基因组数据库的新范式。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 DOI: 10.1016/j.xplc.2025.101494

Fangping Li, Jiaxuan Chen, Wei Luo, Jieying Liu, Guodong Chen, Binyu Shuai, Zhuangwei Hou, Zhenpeng Gan, Hongyuan Zhao, Penglin Zhan, Changwei Bi, Zefu Wang, Haifei Hu, Shaokui Wang

引用次数: 0

Coumarin-facilitated iron transport: An IRT1-independent strategy for iron acquisition in Arabidopsis thaliana. 香豆素促进铁转运：拟南芥铁获取的IRT1独立策略。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-06-25 DOI: 10.1016/j.xplc.2025.101431

Kevin Robe, Max J J Stassen, Shunsuke Watanabe, Javier Espadas, Philippe Gonzalez, Alice Rossille, Meijie Li, Sonia Hem, Aurélien Roux, Véronique Santoni, Joseph Chamieh, Christian Dubos, Esther Izquierdo

{"title":"Coumarin-facilitated iron transport: An IRT1-independent strategy for iron acquisition in Arabidopsis thaliana.","authors":"Kevin Robe, Max J J Stassen, Shunsuke Watanabe, Javier Espadas, Philippe Gonzalez, Alice Rossille, Meijie Li, Sonia Hem, Aurélien Roux, Véronique Santoni, Joseph Chamieh, Christian Dubos, Esther Izquierdo","doi":"10.1016/j.xplc.2025.101431","DOIUrl":"10.1016/j.xplc.2025.101431","url":null,"abstract":"Iron (Fe) is an essential micronutrient for plant growth and development. Despite its importance, Fe uptake in alkaline soils is challenging for most plants because of its poor bioavailability. Plants have evolved two main strategies to acquire Fe. Grass species release phytosiderophores (PS) into the rhizosphere and take up Fe as Fe(III)-PS complexes via specific transporters (strategy II). Non-grass species, such as Arabidopsis thaliana, reduce Fe(III) to Fe(II) at the root surface and transport Fe(II) into the root via the high-affinity transporter IRT1 (strategy I). Additionally, these species secrete catechol coumarins, such as fraxetin, into the rhizosphere to enhance Fe acquisition. Although the role of catechol coumarins in Fe reduction has been clearly demonstrated in acidic soils, their functions under alkaline conditions remain unclear. In this study, we demonstrate that, at circumneutral pH, the catechol coumarin fraxetin forms stable complexes with Fe(III). We also demonstrate that fraxetin significantly improves Fe nutrition, even in mutant plants lacking IRT1 and in the presence of the strong Fe(II) chelator ferrozine, suggesting that plants can bypass the conventional Fe(II)-dependent uptake pathway. These findings support the hypothesis that Fe-coumarin complexes are taken up by plant roots in a manner analogous to Fe(III)-PS complexes in grass species, thereby challenging the current paradigm for plant Fe uptake and suggesting a more unified and flexible model in which strategy I plants can utilize Fe(III)-chelating mechanisms similar to strategy II.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101431"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The origin, evolution, and diversification of MADS-box transcription factors in green plants. 绿色植物MADS-box转录因子的起源、进化和多样化。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-07-19 DOI: 10.1016/j.xplc.2025.101462

Jia-Peng Han, Jun-Nan Wan, Zhi-Lin Guan, Han Xu, Qing-Feng Wang, Tao Wan

{"title":"The origin, evolution, and diversification of MADS-box transcription factors in green plants.","authors":"Jia-Peng Han, Jun-Nan Wan, Zhi-Lin Guan, Han Xu, Qing-Feng Wang, Tao Wan","doi":"10.1016/j.xplc.2025.101462","DOIUrl":"10.1016/j.xplc.2025.101462","url":null,"abstract":"MADS-box genes are among the most important regulatory elements in eukaryotes and have undergone substantial diversification during the evolution of green plants (Viridiplantae). Although the evolution and function of MADS-box genes have been studied extensively, our understanding of their origin and diversification in Viridiplantae remains incomplete. Here, we performed systematic analyses of genomic data from 551 representative eukaryotes, focusing on both sequences and protein structures, to clarify the evolutionary history of MADS-box genes across green-plant lineages. Our analyses suggested that plant type I genes have a polyphyletic origin and that the two subfamilies of plant type II genes (i.e., MIKCC and MIKC∗ genes) arose from an ancient gene duplication in the stem group of streptophytes rather than in land plants. The sphere-like structure of MIKCC proteins revealed in chlorophytes and charophytes is likely an ancestral feature associated with physical constraints to tetramerization. The architectures of MIKCC proteins have evolved greatly in land plants and involve significantly shortened C-termini in which key domains are fully exposed, a structural necessity for tetramer formation. We also documented the diversification of MIKCC genes in ferns, which may have served as the raw genetic basis for their dramatic functional diversification in seed plants.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101462"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bZIP transcription factor RISBZ1 balances grain filling and ER stress response in rice grains. bZIP转录因子RISBZ1平衡水稻籽粒灌浆和内质网胁迫响应。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-07-17 DOI: 10.1016/j.xplc.2025.101458

Qi Sun, Erchao Duan, Ruonan Jing, Yulong Ren, Huan Xu, Chuanwei Gu, Wenting Lv, Xiaokang Jiang, Rongbo Chen, Qingkai Wang, Yipeng Zhang, Rushuang Zhang, Hongyi Xu, Yunpeng Zhang, Jiajia Chi, Yunfei Fu, Yun Zhu, Yu Zhang, Binglei Zhang, Xuan Teng, Hui Dong, Xue Yang, Lei Zhou, Yunlu Tian, Xi Liu, Shijia Liu, Xiuping Guo, Cailin Lei, Ling Jiang, Yihua Wang, Jianmin Wan

{"title":"The bZIP transcription factor RISBZ1 balances grain filling and ER stress response in rice grains.","authors":"Qi Sun, Erchao Duan, Ruonan Jing, Yulong Ren, Huan Xu, Chuanwei Gu, Wenting Lv, Xiaokang Jiang, Rongbo Chen, Qingkai Wang, Yipeng Zhang, Rushuang Zhang, Hongyi Xu, Yunpeng Zhang, Jiajia Chi, Yunfei Fu, Yun Zhu, Yu Zhang, Binglei Zhang, Xuan Teng, Hui Dong, Xue Yang, Lei Zhou, Yunlu Tian, Xi Liu, Shijia Liu, Xiuping Guo, Cailin Lei, Ling Jiang, Yihua Wang, Jianmin Wan","doi":"10.1016/j.xplc.2025.101458","DOIUrl":"10.1016/j.xplc.2025.101458","url":null,"abstract":"In cereal crops, the endosperm synthesizes large quantities of proteins, including storage proteins and functional factors essential for the accumulation of storage reserves. The unfolded protein response (UPR) monitors the folding of nascent polypeptides in the endoplasmic reticulum (ER) to alleviate cellular stress. However, the molecular mechanisms linking the UPR to endosperm development in plants remain poorly understood. In this study, we isolated and characterized a rice (Oryza sativa L.) mutant with defective endosperm development, designated as floury endosperm27 (flo27). Molecular cloning revealed that FLO27 encodes RISBZ1/bZIP58, an endosperm-specific transcription factor co-expressed with seed storage protein (SSP) genes and starch biosynthesis-related genes in rice. The flo27 mutant exhibited severe ER stress, along with upregulation of UPR-related genes. Notably, RISBZ1 interacts with bZIP50 and bZIP60 to antagonistically suppress downstream UPR genes. These interactions simultaneously downregulate SSP and starch biosynthesis-related genes, ultimately leading to reduced dry matter accumulation. In conclusion, these findings demonstrate that RISBZ1 functions as a \"brake signal\" to mitigate ER stress, thus enhancing our understanding of the trade-off between grain filling and adaptation to adverse environmental conditions in rice.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101458"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TaMYB44-TaMYB1 module regulates grain amylose biosynthesis and flour viscosity in wheat. TaMYB44-TaMYB1模块调节小麦籽粒直链淀粉生物合成和面粉粘度。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-07-19 DOI: 10.1016/j.xplc.2025.101461

Sen Li, Guoyu Liu, Ziyan Wu, Dongxue Zhang, Chongyi Gan, Baoyun Li, Jie Liu, Zhongfu Ni, Qixin Sun, Rongqi Liang

{"title":"The TaMYB44-TaMYB1 module regulates grain amylose biosynthesis and flour viscosity in wheat.","authors":"Sen Li, Guoyu Liu, Ziyan Wu, Dongxue Zhang, Chongyi Gan, Baoyun Li, Jie Liu, Zhongfu Ni, Qixin Sun, Rongqi Liang","doi":"10.1016/j.xplc.2025.101461","DOIUrl":"10.1016/j.xplc.2025.101461","url":null,"abstract":"Starch content and composition in wheat endosperm are critical determinants of wheat yield and quality. Amylose content is a key parameter used to characterize starch viscosity in wheat flour, which influences pasting time, peak viscosity, and, consequently, the eating quality of flour-based foods. However, the transcriptional regulatory mechanisms governing amylose biosynthesis in wheat remain poorly understood. Here, yeast one-hybrid (Y1H) screening identified a MYB transcription factor, TaMYB44-4D, that binds to the TaWx-7A promoter. Electrophoretic mobility shift assays (EMSAs), Y1H assays, and transactivation assays demonstrated that TaMYB44-4D binds to the TaWx-7A promoter and represses its transcriptional activity. Knockout (KO) of TaMYB44-A/B/D simultaneously increases amylose content, total starch content, swelling power, and rapid viscosity analyzer parameters, whereas overexpression of TaMYB44-4D reduces these traits. Our results indicate that TaMYB44 functions as a transcriptional repressor of starch biosynthesis. We further confirmed that TaMYB44 interacts with TaMYB1; similar phenotypes were observed in tamyb44-KO and tamyb1-KO lines, as well as in TaMYB44-4D-overexpression (OE) and TaMYB1-6B-OE lines, suggesting that the two factors act as co-repressors. A single-nucleotide polymorphism (T/C) identified in the TaMYB44-D promoter among various wheat varieties is associated with differential TaMYB44 expression levels. Phenotype-genotype association analysis in a natural wheat population revealed that varieties with the CC haplotype exhibit higher amylose content than those with the TT haplotype. In conclusion, our findings provide important insights into the transcriptional regulation of amylose biosynthesis and offer a genetic framework for the improvement of wheat starch quality.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101461"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broad application of the ALFA tagging technology for in planta nanobody-based imaging and biochemical characterization of plant proteins. ALFA标记技术在植物纳米体成像和植物蛋白生化表征中的广泛应用。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-06-27 DOI: 10.1016/j.xplc.2025.101434

Julie Neveu, Julien Spielmann, Steven Fanara, Charlotte Delesalle, Sylvain Cantaloube, Grégory Vert

{"title":"Broad application of the ALFA tagging technology for in planta nanobody-based imaging and biochemical characterization of plant proteins.","authors":"Julie Neveu, Julien Spielmann, Steven Fanara, Charlotte Delesalle, Sylvain Cantaloube, Grégory Vert","doi":"10.1016/j.xplc.2025.101434","DOIUrl":"10.1016/j.xplc.2025.101434","url":null,"abstract":"Epitope tags are widely used for detecting, modifying, or purifying proteins of interest, but their range of application is often limited. Recently, the rationally designed ALFA tag and its ALFA nanobody have expanded the repertoire of epitope tags and emerged as a highly versatile system characterized in various animal models, outperforming existing tags. Here, we evaluated the ALFA tag/ALFA nanobody technology in plants and demonstrated its application for in planta protein detection across multiple compartments and cellular structures, protein-protein interaction studies, protein immunoprecipitation, induced-proximity approaches, and super-resolution microscopy. Most importantly, we highlight the potential of the ALFA tagging technology for proteins that are difficult to tag due to topological or functional constraints. We provide proof of concept for the ALFA tag technology in the detection and functional analysis of the Arabidopsis IRT1 Fe transporter. Overall, this versatile and validated toolbox of ALFA tag and ALFA nanobody applications will serve as a valuable resource for functional studies in plants.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101434"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydroxylation of HPPD facilitates its PUB11-mediated ubiquitination and degradation in response to oxidative stress in Arabidopsis. 在拟南芥中，HPPD的羟基化促进了pub11介导的泛素化和氧化应激的降解。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 DOI: 10.1016/j.xplc.2025.101521

Xin-He Yu, Xun Wen, Jiangqing Dong, Ya-Fang Hu, Xin-Long Wang, Dan-Yi Zhu, Qihua Ling, Hong-Yan Lin, Guang-Fu Yang

{"title":"Hydroxylation of HPPD facilitates its PUB11-mediated ubiquitination and degradation in response to oxidative stress in Arabidopsis.","authors":"Xin-He Yu, Xun Wen, Jiangqing Dong, Ya-Fang Hu, Xin-Long Wang, Dan-Yi Zhu, Qihua Ling, Hong-Yan Lin, Guang-Fu Yang","doi":"10.1016/j.xplc.2025.101521","DOIUrl":"https://doi.org/10.1016/j.xplc.2025.101521","url":null,"abstract":"4-Hydroxyphenylpyruvate dioxygenase (HPPD) plays a critical role in plant photosynthesis, and is essential for enhancing tolerance to oxidative stress. However, the precise mechanisms through which plants regulate HPPD in response to oxidative stress remain largely unknown. Here, we report that the Arabidopsis thaliana HPPD (AtHPPD) undergoes an uncharacterized post-translational modification, namely phenylalanine hydroxylation, in response to excessive hydroxyl radicals (·OH), thereby mediating oxidative stress tolerance. Biochemical analyses reveal that this hydroxylation impairs the normal function of AtHPPD, leading to its accelerated degradation. Furthermore, we identified PUB11 as a key interactor with AtHPPD. In vitro and in vivo assays show that PUB11 enhances its interaction with AtHPPD under oxidative stress, promoting ubiquitination and facilitating rapid degradation of AtHPPD via the 26S proteasome pathway to balance the ROS levels. Overall, this work provides new insights into how plants balance photosynthetic efficiency and the repair of oxidative damage, while uncovering key processes in oxidative stress regulation, providing a foundation for crop breeding to meet abiotic challenges.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101521"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A homeobox transcription factor HB34 suppresses jasmonic acid biosynthesis but promotes the expression of growth-related genes to balance plant immunity and growth in Arabidopsis. 同源盒转录因子HB34抑制拟南芥茉莉酸的生物合成，但促进生长相关基因的表达，以平衡植物免疫和生长。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-06-24 DOI: 10.1016/j.xplc.2025.101429

Xiaoxiao Li, Jinwei Yu, Wei Zhou, Fei Yan, Chuyu Lin, Zeng Tao

{"title":"A homeobox transcription factor HB34 suppresses jasmonic acid biosynthesis but promotes the expression of growth-related genes to balance plant immunity and growth in Arabidopsis.","authors":"Xiaoxiao Li, Jinwei Yu, Wei Zhou, Fei Yan, Chuyu Lin, Zeng Tao","doi":"10.1016/j.xplc.2025.101429","DOIUrl":"10.1016/j.xplc.2025.101429","url":null,"abstract":"Activation of plant immunity is commonly associated with the biosynthesis of defense-related metabolites, such as jasmonic acid (JA) and salicylic acid (SA). However, constitutive activation of the immune response or excessive accumulation of defense metabolites often negatively impacts plant growth. The regulatory mechanisms underlying the trade-off between plant immunity and growth remain elusive. In this study, we identified a homeobox transcription factor, HB34, as a key regulator that balances plant immunity and growth by differentially regulating the expression of JA biosynthetic and growth-related genes in Arabidopsis. Loss of HB34 enhances plant resistance to the necrotrophic pathogen Botrytis cinerea but impairs plant growth and development, accompanied by constitutive activation of JA-responsive genes. Mechanistically, HB34 negatively regulates the transcription of JA biosynthetic genes to suppress JA accumulation, and blocking JA biosynthesis attenuates the enhanced Botrytis cinerea resistance of hb34. Conversely, HB34 enhances the transcription of growth-related genes, whereas overexpression of these genes partially rescues growth defects, thereby decoupling the trade-off between enhanced defense and impaired plant growth. Our findings reveal a novel mechanism whereby a single transcription factor fine-tunes the trade-off between plant growth and immunity by differentially regulating JA biosynthetic and growth-related genes in Arabidopsis.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101429"},"PeriodicalIF":11.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extending Mendel's legacy: Application of hawkweed PpPAR for inducing synthetic apomixis in hybrid rice. 孟德尔遗产的延续：山楂PpPAR在杂交水稻诱导合成无杂交中的应用。

IF 11.6 1区生物学

Plant Communications Pub Date : 2025-09-08 Epub Date: 2025-05-27 DOI: 10.1016/j.xplc.2025.101387

Jie Xiong, Yajie Ji, Shenlin Yang, Yong Huang, Xianjin Qiu, Qian Qian, Charles J Underwood, Kejian Wang

引用次数: 0