World''s Veterinary Journal最新文献

{"title":"Effects of Nano Zinc on Growth Performance, Health Status, and Cecal Microbiota in Broiler Chickens Challenged with Salmonella Kentucky","authors":"A. El-Shenawy, A. Salim, M. Gouda","doi":"10.54203/scil.2022.wvj14","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj14","url":null,"abstract":"Public concern with the incidence of antibiotic-resistant bacteria, particularly among foodborne pathogens, such as Salmonella, has been challenging the poultry industry to find alternative means of control. The present study was conducted to investigate the effect of dietary replacement of inorganic zinc oxide (ZnO) by different levels of zinc nanoparticles on growth performance, blood serum biochemical changes, immune response, cecal microbiota, and some internal organs histopathology of Salmonella Kentucky (SK) challenged broiler chickens. A total of 180 one-day-old broiler chicks were used in the present experiment. The chicks were randomly allotted into six equal groups (30 chicks/group), with 3 subgroups containing 10 chicks as a replicate. The first group fed on the basal diet supplemented by 100 mg ZnO/kg diet, while the second and the third groups fed on the basal diet with replacement of ZnO by 100 and 50 mg of zinc oxide nanoparticles (ZnONPs)/kg diet, respectively. Moreover, the fourth, fifth, and sixth groups fed as the first three groups with SK challenge on the third day of age. Results showed that supplementation of 100 mg ZnONPs/kg diet instead of ZnO reduced the severity of the clinical signs, post-mortem lesions, mortality, and SK fecal shedding of SK challenged chicks. Replacement of ZnO by 100% or 50% of ZnONPs increased cecal total bacterial counts and lactobacillus bacterial count while reducing total coliform counts. On the other hand, the SK challenge increased cecal total bacterial counts and lactobacillus bacterial counts, compared to the broiler chicks group fed on the diet without SK challenge. The SK challenge with inorganic zinc addition reduced body gain and feed conversion ratio, while 100 or 50 mg ZnONPs/kg diet supplementation instead of ZnO improved growth performance, feed efficiency parameters. It was observed that the replacement of inorganic zinc (serum ZnO) by 100 mg /kg diet significantly increased lysosomal and phagocytic activity by about 261.5% and 17.9%, respectively. Moreover, 100% or 50% of ZnONPs instead of inorganic zinc significantly ZnONPs increased liver, spleen, and thymus gland relative weights of SK-challenged broiler chickens, compared to broiler chickens group fed on the same diet without challenge or compared to chicks group fed on ZnO supplemented diet with SK challenge, while replacement of inorganic zinc (ZnO) by 100 or 50mg ZnONPs/kg diet reduced the adverse effect.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47724853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Detrimental Effects of Alcohol Consumption on Infertility of Humans and Laboratory Animals: A Review","authors":"Made Nyandra, I. G. Widhiantara, P. Wiradana","doi":"10.54203/scil.2022.wvj1","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj1","url":null,"abstract":"In recent decades, the decline in human fertility has become a major concern. However, unhealthy lifestyle practices, such as the use of addictive substances that contribute to infertility persist in society. Therefore, the current study reviewed the literature addressing the effects of alcohol consumption patterns on male and female fertility. Although alcohol intake is socially accepted, its detrimental influence on male and female fertility rates has been regularly observed in recent years. The findings have revealed that abstinence from chronic alcohol intake did not recover the testes from the negative effects of alcohol. Heavy drinking, defined as 8 or more glasses per week for a woman or 15 or more drinks per week for a man might impair female reproductive function. In conclusion, the implementation of an in vivo evidence strategy ranging from animal studies to preclinical ones has indicated that alcohol intake may be related to negative effects on reproductive parameters in both males and females. The present review deserves to be highlighted since it is significant for those who lead an unhealthy lifestyle, such as those who use alcohol.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45972455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Evaluation and Molecular Discrimination of Milk Samples from Humans and Different Animals","authors":"A. S. M. Abuelnaga, N. Ata, K. A. ABD EL-RAZIK, R. H. Hedia, M. Soliman, M. Kandil, E. Elgabry, A. Arafa","doi":"10.54203/scil.2022.wvj2","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj2","url":null,"abstract":"Milk is a highly nutritious food and it is important to be free of any pathogenic microbes that could be transmitted to humans and affect public health. A total of 145 milk samples were collected from humans and different animal species (cow, buffalo, ewe, goat, camel, mare, and donkey) and underwent physical examination (color, odor, and taste), chemical analysis for its components (water, total salt, fat, protein, lactose, and ash), and finally microbiological (bacteriological and mycological) examinations. Standard plate count, preliminary incubation count, lab pasteurized count, coliform, Escherichia coli, Staphylococcus species, Salmonella species, yeast, and mold counts were measured. PCR test was performed to differentiate milk from different sources (animals and humans) by producing a specific band for each milk type. The results of the physical examination of different kinds of milk showed different grades of white color with the characteristic odor and taste of each milk type. Chemical examination revealed that the highest water content was in donkey milk and the lowest was in buffalo milk while total solids indicated the highest content in sheep milk and the lowest in donkey milk. The microbiological analysis presented that the standard plate count results were the highest in the milk obtained from sheep and camel, while donkey milk was the least in this regard. Yeast counts were the highest in buffalo milk but cow milk was the highest in mold counts. PCR results of milk types using species-specific primers and DNA template extracted from milk somatic cells revealed a specific band for each milk type as 157, 195, 225, 242, 274, and 711 base pair (bp) for goat, human, sheep, buffalo, cattle milk, and camel milk, respectively. It was concluded that more restrictions must be applied to decrease milk contamination as high microbial counts detected in the present study can affect milk quality, public health, and the dairy industry. PCR used in the current work for milk discrimination used milk somatic cells specifically mitochondrial cytochrome b gene which exhibited high specificity in the PCR reactions and this could be served as a cheap and simple method, compared to other types of PCR.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43218130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human and Canine Leishmaniasis: Diagnosis and Risk Factors","authors":"A. Dahmani, N. Ouchene-khelifi, N. Ouchene","doi":"10.54203/scil.2022.wvj7","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj7","url":null,"abstract":"Leishmaniasis is a zoonosis disease caused by a parasite of the genus Leishmania transmitted by the sandflies. It is ranked among the 20 major neglected tropical diseases. Algeria is classified as one of the most affected countries by cutaneous leishmaniasis worldwide. This study was conducted in the Medea region, north-central Algeria, to investigate human and canine leishmaniasis. Diagnosis of human leishmaniasis was conducted on patients with symptoms suggestive of leishmaniasis. The presence of wet or dry lesions, number, location, duration, and travel history were recorded for each patient. Confirmation of the disease was performed by histopathological test. Canine leishmaniasis was diagnosed on the basis of clinical examination in 175 male Sloughi dogs. Symptoms included skin ulcerations, lymphadenopathy, dermatitis with alopecia, weight loss, and ocular or nasal lesions. A total of 1070 cases of human leishmaniasis were investigated, including 1067 (99.72%) cases of cutaneous leishmaniasis and 3 (0.28%) cases of visceral leishmaniasis. Of the 1067 human cutaneous leishmaniasis cases, 59.51% and 40.49% were male and female, respectively. For visceral leishmaniasis, all cases were male. Persons aged less than 10 years were more infected than those over 10 years of age. Chahbounia region was found to be the most infected area, compared to other regions. The highest number of human leishmaniasis cases was recorded during November (462 cases). Human cutaneous leishmaniasis was the most frequent (81.38%) among the other human pathologies in the study area; followed by pulmonary tuberculosis (14.8%). All Sloughi dogs were diagnosed with leishmaniasis of which the most common symptoms included lymph node hypertrophy, emaciation, skin lesions, fever, epistaxis, alopecia, ocular lesions, anemia, onychogryphosis, chemosis, and the less common symptoms are: fever, diarrhea, and splenomegaly. The Psammomys obesus and Meriones shawi were present especially in the periphery of the lands next to the valley of Chahbounia city. Leishmaniasis remains present in the region, constituting a public health menace. The union of veterinary and public services is necessary to eradicate the disease by controlling the vector and the reservoirs of the parasite.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42470119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Dietary Inclusion of Miana Plant Flour (Plectranthus scutellarioides (L.) R. Br. on Serum Lipid Profile and Organ Weights of Broiler Chickens","authors":"M. Mahata, Misra Weni, Yola Gusnanda, T. Ohnuma, Y. Rizal","doi":"10.54203/scil.2022.wvj6","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj6","url":null,"abstract":"Miana plant (Plectranthus scutellarioides (L.) R. Br. contains active compounds (such as steroids, flavonoids, saponins, and tannins) which can have several health benefits, including lowering cholesterol LDL and triglyceride as well as increasing feed consumption, body weight, and carcass weight of broilers. Therefore, the current experiment was conducted to evaluate the effect of Miana plant flour (Plectranthus scutellarioides (L.) R. Br. in the diet on blood serum lipid profiles and physiological organs of broilers. The experiment was performed on 100 day-old broiler chickens from strain Arbor Acres CP-707. The experiment was designed in a completely randomized design with five different levels of Miana plant flour (0%, 5%, 7.5%, 10%, and 12.5%) in broiler's diets as treatment, and each treatment was repeated four times. The diet was arranged iso-protein (21%) and iso-energy (2900 kcal/kg). The serum lipid profile measurement included the analysis of total cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL). Physiological organ analysis entailed the percentage of liver weight, pancreas weight, gizzard weight, small intestine weight, and length of parts of the small intestine (duodenum, jejunum, and ileum) of broilers. The results showed that the inclusion of Miana plant flour in the broiler's diet could significantly affect total cholesterol, triglycerides, HDL, and LDL in the serum of broiler chickens, and it affected duodenum length significantly. Furthermore, the inclusion of Miana plant flour in the broiler's diet had an insignificant effect on the percentage of liver weight, pancreas weight, gizzard weight, small intestine weight, and length of each part of the small intestine (jejunum and ileum) on broilers. In conclusion, the inclusion of Miana plant flour as much as 12.5% in broiler's diets reduced total cholesterol, triglycerides, and LDL, and increased the HDL and duodenum length without adverse effects on the other physiological organs of broiler chickens.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47729858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Morphological Traits to Predict Body Weight of Dorper Sheep Lambs","authors":"L. J. Selala, Thobela Louis Tyasi","doi":"10.54203/scil.2022.wvj9","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj9","url":null,"abstract":"The Dorper sheep are known to be a fast-growing breed with a very good body conformation that produces high-quality carcass. The recent study was conducted to determine the relationship between body weight (BW) and morphological traits, such as heart girth (HG), rump height (RH), body length (BL), withers height (WH), and sternum height (SH). A total of 51 Dorper sheep lambs (29 female and 22 male lambs) were used as experimental animals. The data was collected 24 hours after birth. Data were analyzed using Pearson’s correlation and simple regression to attain the objectives. The obtained results indicated that BW had a positively high statistically correlation with HG (r = 0.81), RH (r = 0.766), BL (r = 0.893), WH (r = 0.874), and SH (r = 0.618) in female Dorper sheep lambs. Furthermore, results showed that BW had a positively high statistically significant association with HG (r = 0.886), RH (r = 0.590), BL (r = 0.900), WH (r = 0.613), and SH (r = 0.707) in male Dorper sheep lambs. Simple regression models for morphological traits indicated that BL had the highest coefficient of determination (R2 = 0.80) and the lowest mean square error (MSE = 2.83) in female Dorper sheep lambs, and also the highest coefficient of determination (R2 = 0.81) and mean square error (MSE = 1.07) in male Dorper sheep lambs. In conclusion, the findings indicated that improving HG, RH, BL, WH, and SH might result in the enhancement of BW in Dorper sheep lambs. Simple regression results suggested that BL could be selected as a facilitating factor in the breeding programs to improve the BW of Dorper sheep lambs at birth.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44928510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Replacing Soybean Meal with Sunflower Meal, Sesame Meal, and Black Seed Meal in diets of Barki Lambs","authors":"Reham Roshdy Ali El-Tanany, Abderrahim Belkasim Ali Chiab, Hesham Mohamed Abd EL-Gawad El-Banna, Ali Mohamed Ali Mostafa, A. Mahmoud","doi":"10.54203/scil.2021.wvj84","DOIUrl":"https://doi.org/10.54203/scil.2021.wvj84","url":null,"abstract":"Crude protein content in animal diet is considered the first important factor for nutritionists when they start to formulate a diet for different types of animals. Therefore, this experiment was conducted to investigate the effect of substituting soybean seed meal by different protein sources on lamb’s diet in terms of nutrients digestibility, and growth performance. Therefore, four tested concentrate feed mixtures (CFM, 14% crude protein) were designed. The CFM1 contained soybean meal as the only source of protein, and 50% of soybean meal protein was replaced by either sunflower meal, sesame seed meal, or black seed meal in CFM2, CFM3, and CFM4 groups, respectively. A total of 28 growing Barki male lambs aged 6 months with an average body weight of 38.6 ± 0.4 Kg were used in feeding trials for 120 days. Depending on their body weight, lambs were divided into four groups (7 animals in each). Each group fed on one of the experimental diets, including D1 (CFM1+ clover hay), D2 (CFM2 + clover hay), D3 (CFM3 + clover hay), and D4 (CFM4 + clover hay). Results of digestion coefficients indicted that the digestibility of crude protein and the nitrogen-free extract was significantly higher for D1 and D4, compared to D2 and D3. The same trend was observed in nutritive values expressed as total digestible nutrients (TDN) and digestible crude protein (DCP), whereas the D1 and D4 recorded higher TDN and DCP, compared to D2 and D3. Results of rumen parameters demonstrated that there were insignificant differences among groups regarding the average pH, ammonia (NH3-N), and total volatile fatty acids (TVFAs). Data of growth performance indicated that the final live body weight, total body gain, and average daily gain of lambs feed D4 (56.75 kg, 18.62 kg, and 155 g, respectively) and D1 (56.62 kg, 17.60 kg, and 147 g, respectively) were higher than those for lambs fed D2 (53.40 Kg, 14.64 kg, and 122 g, respectively) and D3 (53.11 kg, 14.61 kg, and 122 g, respectively). The feed conversion ratio (feed/gain) was recorded better value with lambs fed D4 followed by that fed D1. In conclusion, black seed meal could be replaced with the 50% of soybean meal participation of protein in growing Barki lambs’ diets without any adverse effects on digestibility, rumen kinetics, and growth performance.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43173659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adding Kaffir Lime (Citrus hystrix) Leaf Essential Oil to Gelatin Coating for Extending the Shelf Life of Red Snapper Fillet","authors":"Ika Febriana, Endang Dewi Mashithah, H. Pramono","doi":"10.54203/scil.2021.wvj91","DOIUrl":"https://doi.org/10.54203/scil.2021.wvj91","url":null,"abstract":"Red Snappers contain high content protein and water which makes them highly perishable. However, they are widely processed into the fillet. One of the disadvantages of filleting is the short shelf life due to the high water content turning the fillets into an ideal media for bacteria. The edible coating protects the meat from perishability by reducing the water vapor, aroma, and lipid migration. One of the potential protein sources for coating is gelatin. The essential oil content that has a role in inhibiting bacterial growth is citronellal. The present study aimed to determine the effects of adding Kaffir limes (Citrus hystrix) leaf essential oil to a gelatin coating on the shelf life of red snappers’ fillet. An experiment was conducted using a completely randomized design with four treatment groups based on the concentration of Citrus hystrix essential oil and five replications (n = 3 per group). In this regard, the treatments were named P0 (0% of Citrus hystrix), P1 (1% of Citrus hystrix), P2 (1.5% of Citrus hystrix), and P3 (2% of Citrus hystrix). The investigated parameters included total plate count, sensory quality, and acidity. Data analysis was performed using ANOVA followed by Duncan’s multiple range test. The results showed that the addition of 1.5% and 2% Citrus hystrix essential oil decreased the number of bacteria, and led to a higher sensory quality. In conclusion, The addition of Citrus hystrix essential oil in the gelatin-coating process can be used as a method of processing the fishery products to extend the shelf life of red snappers’ fillet at room temperature and inhibit bacterial growth.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44979318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Chitosan from Shrimp Waste for Treating Staphylococcus aureus Bacteria in Skin Wound","authors":"I. Widnyana, S. Subekti, K. .","doi":"10.54203/scil.2021.wvj88","DOIUrl":"https://doi.org/10.54203/scil.2021.wvj88","url":null,"abstract":"Shrimp or crustacean waste on the skin, head, and feet has not been maximally utilized. Shrimp or crustacean waste can be processed into chitin or chitosan which can provide high added value. Chitosan is one ingredient that can be used to make skin ointments. One of the bacteria that is harmful to the skin during a wound is Staphylococcus aureus. Hence, there is a need to have a skin ointment that can inhibit these bacteria. The current study aimed to evaluate the antibacterial activity of chitosan in the ointment. Moreover, the current study planned to determine the optimum inhibitory concentration of chitosan in the ointment against Staphylococcus aureus and evaluate the physical properties of ointments. The methods used in the current study were the pH determination, organoleptic test, homogeneity test, and antibacterial activity test to examine chitosan against Staphylococcus aureus. The results showed that chitosan in ointment had a weak antibacterial activity with a value of < 5 mm inhibition zone. Chitosan with a concentration of 0.2% in ointment fulfilled the requirements of a good ointment. Its white colour as the typical colour of chitosan showed the characteristic odour of chitosan and a semi-solid form. Chitosan in the ointment has antibacterial activity that can inhibit Staphylococcus aureus bacteria.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46333168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection","authors":"T. Untari, Y. P. Kristianingrum, A. Kusumawati","doi":"10.54203/scil.2021.wvj89","DOIUrl":"https://doi.org/10.54203/scil.2021.wvj89","url":null,"abstract":"Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43593994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}