实时荧光定量PCR检测牛疱疹病毒1型在预防传染性牛鼻气管炎传播中的应用

Q4 Veterinary

World''s Veterinary Journal Pub Date : 2021-12-25 DOI:10.54203/scil.2021.wvj89

T. Untari, Y. P. Kristianingrum, A. Kusumawati

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引用次数: 0

摘要

传染性牛鼻气管炎（IBR）可通过牲畜种子和精液通过人工授精过程传播。因此，有必要通过印度尼西亚的人工授精和繁殖中心检测精液中是否存在牛疱疹病毒1型（BHV1）。目前的研究旨在检测精液中是否存在病毒作为印尼IBR疾病传播的来源。使用实时PCR（qPCR）技术对来自印度尼西亚人工授精和繁殖中心（Sembawa、Lembang、Ungaran和Sleman）的27份精液样本进行了检测和鉴定。结果表明，所有样品对BHV1均呈阴性反应。这表明，Ungaran、Sembawa、Sleman和Lembang的精液可以安全地用作人工授精的精液来源。

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Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection

Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.

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来源期刊

World''s Veterinary Journal Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： The World''s Veterinary Journal (ISSN 2322-4568) is an international, peer reviewed open access journal aims to publish the high quality material from veterinary scientists'' studies. All accepted articles are published Quarterly in full text on the Internet. WVJ publishes the results of original scientific researches, reviews, case reports and short communications, in all fields of veterinary science. In details, topics are: Behavior Environment and welfare Animal reproduction and production Parasitology Endocrinology Microbiology Immunology Pathology Pharmacology Epidemiology Molecular biology Immunogenetics Surgery Virology Physiology Vaccination Gynecology Exotic animals Animal diseases Radiology Ophthalmology Dermatology Chronic disease Anatomy Non-surgical pathology issues of small to large animals Cardiology and oncology.