{"title":"Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis.","authors":"Angsana Keeratijarut, Patcharee Karnsombut, Rangsima Aroonroch, Somboon Srimuang, Tumtip Sangruchi, Lalana Sansopha, Piroon Mootsikapun, Noppadol Larbcharoensub, Theerapong Krajaejun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pythiosis, a life-threatening infectious disease of humans and animals in tropical and subtropical countries, is caused by the fungus-like organism Pythium insidiosum. As diagnosis of pythiosis is difficult, delayed diagnosis of pythiosis leads to poor prognosis. We developed an immunoperoxidase staining assay using rabbit anti-P. insidiosum antibodies to detect P. insidiosum directly in infected tissues of 19 patients with vascular (n = 11), ocular (n = 7) or cutaneous (n = 1) pythiosis. Tissue sections from 31 patients with various fungal infections were included as controls. Tissue sections from all pythiosis patients and 2 patients with Fusarium infections were stained positive, whereas the other 29 control sections were stained negative. Sensitivity and specificity of the assay was 100% and 94%, respectively. Based on the prevalence of human pythiosis (2%), calculated positive predictive value and negative predictive value was 24% and 100%, respectively. Thus, the diagnostic value of this assay is for ruling out pythiosis. The assay requires routine laboratory equipments and can easily be performed by pathologists in rural hospitals where the disease is more prevalent.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1298-305"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hemoptysis in children with pandemic influenza H1N1 2009 infection.","authors":"Lucksamee Haura, Boonyarat Warachit, Jarika Makkoch, Yong Poovorawan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three patients were admitted to Hat Yai Hospital, Songkhla Thailand with hemoptysis. They were previously healthy children aged 6, 13, and 14 years old who had attended schools in which outbreaks of influenza had occurred. They all had a history of fever, rhinorrhea, and severe cough accompanied by hemoptysis. Two developed hemoptysis on Day 3 and the third on Day 6 of illness, with one of them displaying massive hemoptysis. Chest radiographs were compatible with viral pneumonia in two cases and the third case was unremarkable. Coagulation profiles in the severe case were carried out and were normal. All the patients responded very well to treatment with oseltamivir and did not require intubation. Their subsequent nasopharyngeal swabs were positive for human pandemic influenza A H1N1 by real-time reverse transcription-polymerase chain reaction (RT-PCR), and their sputum for acid-fast bacilli and tuberculin skin tests were negative.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1259-63"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of single-tube mutiplex RT-PCR for dengue virus typing.","authors":"Pannamas Maneekan, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Jaranit Kaewkangwal, Natthanej Luplertlop","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1254-8"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Pongpiachan', K Thamanu, K F Ho, S C Lee, P Sompongchaiyakul
{"title":"Predictions of gas-particle partitioning coefficients (K(P)) of polycyclic aromatic hydrocarbons at various occupational environments of Songkhla Province, Thailand.","authors":"S Pongpiachan', K Thamanu, K F Ho, S C Lee, P Sompongchaiyakul","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ambient air samples were collected over periods of 24 hours and 3 hours using a Graseby-Anderson high volume air sampler with PM10 TE-6001 at 15 sampling sites, including an urban residential zone, rural area, industrial factories, waste incinerator, traffic areas and agricultural burning areas in Songkhla Province, Thailand. An analysis of organic carbon and elemental carbon was conducted to estimate the gas-particle partitioning of PAHs using a model proposed by Dachs-Eisenreich. The estimated LogK(P) of PAHs emphasized the risk for lung cancer among outdoor workers in waste incinerators, traffic intersections and bus terminals, suggesting the role of the carbonaceous fraction over the gas-particle partitioning of PAHs. Analysis of f(SC)K(SA)delta(OCT)/F(OM)K(OA) ratios revealed a significant role of adsorption and absorption in unusually high elemental carbon fractions (ie low OC/EC ratio) in fine particles collected at waste incinerators and the abnormally high organic carbon fractions (ie high OC/EC ratio) observed in those fine particles of an urban residential zone, respectively. The dual mode of ab/adsorption tended to dominate the gas-particle partitioning of LMW PAHs collected at the traffic intersections and bus terminal.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1377-94"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29081426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of different transfusion regimens on craniofacial appearance and dentition in severe thalassemic children.","authors":"Varangkanar Jirarattanasopa, Phakatiti Hooncharoen, Arunotai Mekaewkunchorn, Kitti Torcharus","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thalassemia is a group of inherited diseases with a defect in the synthesis of hemoglobin. Severe thalassemic subjects suffer from craniofacial deformities and malocclusion due to bone marrow hyperplasia compensating for ineffective erythropoiesis. Blood transfusions are used to maintain life and reduce complications. The transfusions may have benefits in reducing craniofacial and dentition abnormalities. However, appropriate therapy is still controversial. This study evaluated the effect of different transfusion regimens on craniofacial appearance and dentition. Ninety-two severe thalassemic patients, aged 6 -13 years, were divided into 3 groups according to the frequency of transfusion: 1) high transfusion: more than 12 times/year. 2) low transfusion: 6-12 times/year. 3) occasional transfusion: less than 5 times/year. The appearance and dentition were evaluated and compared among groups. Most subjects in the high transfusion group had a normal appearance and a class I molar and incisor relationship with normal overjet and overbite. More than half of subjects in the low and occasional groups showed craniofacial abnormalities and malocclusion, particular in the occasional group. Frequency of transfusion has an effect on craniofacial appearance and dental occlusion; only high frequent transfusions were effective in preventing craniofacial and dental defects.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1371-6"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rashidi Ahmad, Rashdan Rahmat, Nik Hisamudin, Nik Abdul Rahman, Abu Yazid Mohd Noh, Nasir Mohammad, Sheik Farid Abdul Wahab, Ida Zarina Zaini
{"title":"Demographic and clinical characteristics of red tag patients and their one-week mortality rate from the emergency department of the Hospital Universiti Sains Malaysia.","authors":"Rashidi Ahmad, Rashdan Rahmat, Nik Hisamudin, Nik Abdul Rahman, Abu Yazid Mohd Noh, Nasir Mohammad, Sheik Farid Abdul Wahab, Ida Zarina Zaini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Early identification and rapid treatment of red tag patients may decrease morbidity and mortality. We examined the clinical characteristics, etiologies and one week mortality rate of red tag (life threatening and potentially life threatening illness) patients at the Hospital Universiti Sains Malaysai (HUSM). A cross-sectional study was conducted at the Emergency Department of the HUSM from 1 August 2006 to 31 January 2007; 440 eligible patients were analyzed. The group had a mean age of 47.2 +/- 22 years, with 67.3% of the patients being male. Twenty-three percent were trauma cases with motor vehicle accident being the major mechanism of injury. Fifty-four percent of the cases had cardiac related illnesses. The mean duration of stay in the Emergency Department (ED) was 3.9 +/- 1.5 hours. The survival rate at one week was 76.6%. The non-trauma group comprised 74.0% of death cases. Acute coronary syndrome and road traffic accidents comprised 22.0% of total death cases at one week. Red tag patients constitute a large proportion of ED cases and may remain in the ED for significant periods of time.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1322-30"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence and risk factors of overweight and obesity in Turkish academic staff.","authors":"Edibe Pirinçci, Süheyla Rahman, Birsen Durmus, Yasemin Açik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Obesity and overweight are a significant public health problem that affects the quality of life of the individuals concerned. We studied the prevalence and related risk factors for obesity among academics. This descriptive cross-sectional study sampled 499 academic staff at Elazig Firat University, Turkey. Height, weight and blood pressure values were obtained and a descriptive survey was conducted. The age range of the participants was 22 to 65 years, with a mean age 36.5 +/- 8.1 years. The group had 29% women, and 71% men; 80% were married. The mean BMI of the participants was 24.6 +/- 3.1 kg/m2. Their mean systolic blood pressure was 117.0 +/- 12.1 mmHg and mean diastolic blood pressure was 73.1 +/- 8.0 mmHg. The overall prevalence of obesity was 7.0%; 2.1% in women and 9.0% in men. The combined prevalence of overweight and obesity was 45.9%. Nearly half the participants were either obese or overweight. A correlation was identified between overweight/obesity and sex, marital status, academic staff, physical activity, systolic and diastolic blood pressure. Although the study did not include all academics, the results reveal the need to evaluate the health impact of obesity in academics.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1306-14"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Undiagnosed amebic brain abscess.","authors":"Parnpen Viriyavejakul, Mario Riganti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report a case of amebic brain abscess due to Entamoeba histolytica. The patient was a 31-year-old man who presented with amebic liver abscess. His clinical course deteriorated in spite of proper drainage and treatment. He developed delirium, lethargy and then expired. With a history of heroin addiction, withdrawal syndrome from heroin was suspected. At autopsy, amebic abscesses were detected in the liver, large intestine, meninges and brain. A 19 cm amebic liver abscess was found in the right lobe of the liver. A 4 cm amebic brain abscess was found in the right occipital lobe. Microscopically, the tissue sections from the affected organs were confirmed to have degenerated E. histolytica trophozoites. Involvement of the brain in amebic liver abscess should be suspected in patients with neurological signs and symptoms.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1183-7"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of curcumin on pathogenesis of hamster-opisthorchiasis through apoptosis-related gene expression.","authors":"Pranee Sriraj, Thidarut Boonmars, Sirintip Boonjaraspinyo, Butsara Kaewsamut, Tuanchai Srisawangwong, Paiboon Sithithaworn, Zhiliang Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study investigated the effect of curcumin, a phenolic compound with yellow color from Curcuma longa L., on the expression of the apoptosis-related genes [BAX (Bcl-2 associated protein X), PKB, p53, MDM2 (mouse double minute 2), caspase 9, c-Ski, smad1 and smad4] in hamster opisthorchiasis. On Opisthorchis viverrini infection treated with dietary curcumin apoptosis-related gene expression profiles were similar to O. viverrini-infected group, but the expression levels seemed lower. Light microscopic observation revealed that aggregation of inflammatory cells surrounding the hepatic bile ducts in the groups infected with O. viverrini and treated with dietary curcumin was lower than in infected group. The intensity of the response is correlated with expression of the genes studied. The results suggest that curcumin reduces pathogenesis in hamster-opisthorchiasis by controlling apoptosis-related gene expression.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1208-15"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular identification of larval trematode in intermediate hosts from Chiang Mai, Thailand.","authors":"Suksan Chuboon, Chalobol Wongsawad","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Snail and fish intermediate hosts were collected from rice fields in 3 districts; Mueang, Mae Taeng and Mae Rim of Chiang Mai Province during April-July 2008. For identification of larval trematode infection, standard (cracked for snail and enzymatically digested for fish) and molecular methods were performed. The results showed that three types of cercariae were found, pleurolophocercus, cotylocercous, and echinostome among 4 species of snail with a prevalence of 29, 23 and 3% respectively. Melanoides tuberculata snail was the most susceptible host for cercariae infection. Four species of metacercariae, Haplorchis taichui, Stellantchasmus falcatus, Haplorchoides sp and Centrocestus caninus, were found with a prevalence of 67, 25, 60 and 20%, respectively. The Siamese mud carp (Henicorhynchus siamensis) was the most susceptible fish host for H. taichui, and half- beaked fish (Dermogenys pusillus) for S. falcatus metacercariae infection, whereas Haplorchoides sp and C. caninus were concomitantly found in Puntius brevis. HAT-RAPD profile confirmed that pleurolophocercus cercariae found in Melanoides tuberculata from Mae Taeng District belonged to H. taichui and in Tarebia granifera from Mueang District were S. falcatus.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1216-20"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}