{"title":"登革病毒单管多重RT-PCR分型方法的建立。","authors":"Pannamas Maneekan, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Jaranit Kaewkangwal, Natthanej Luplertlop","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1254-8"},"PeriodicalIF":0.0000,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of single-tube mutiplex RT-PCR for dengue virus typing.\",\"authors\":\"Pannamas Maneekan, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Jaranit Kaewkangwal, Natthanej Luplertlop\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.</p>\",\"PeriodicalId\":520816,\"journal\":{\"name\":\"The Southeast Asian journal of tropical medicine and public health\",\"volume\":\" \",\"pages\":\"1254-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Southeast Asian journal of tropical medicine and public health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Southeast Asian journal of tropical medicine and public health","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development of single-tube mutiplex RT-PCR for dengue virus typing.
This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
