{"title":"Intramolecular integration assay validates integrase phi C31 and R4 potential in a variety of insect cells.","authors":"Jakkrawarn Chompoosri, Tresa Fraser, Yupha Rongsriyam, Narumon Komalamisra, Padet Siriyasatien, Usavadee Thavara, Apiwat Tawatsin, Malcolm J Fraser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Phage phi C31 and R4 integrases are site-specific and unidirectional serine recombinases. We have analyzed the ability of these integrases to mediate intramolecular integration between their attB and attP sites in 7 important insect cell lines as a means of predicting their relative mobility in the corresponding insect species. Both integrases exhibit significantly higher frequencies in Drosophila S2 cells than in the other insect cell lines examined, but do work well in all of the species tested. Our results, coupled with previous results of the activity of phi C31 integrase in D. melanogaster and Aedes aegypti, suggest the family of serine catalyzed integrases will be useful site-specific integration tools for functional genome analysis and genetic engineering in a wide range of insect species.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1235-53"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jyoti Gulia, Peter Kim, Mark G Kortepeter, Annick Nguewou, Masashi Waga, Leon Lai
{"title":"Multiple fractures of the symphysis pubis due to tuberculous osteomyelitis.","authors":"Jyoti Gulia, Peter Kim, Mark G Kortepeter, Annick Nguewou, Masashi Waga, Leon Lai","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1279-83"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somsak Akksilp, Wanpen Wattanaamornkiat, Wanitchaya Kittikraisak, Sriprapa Nateniyom, Somsak Rienthong, Chawin Sirinak, Keerataya Ngamlert, Wiroj Mankatittham, Wanchai Sattayawuthipong, Surin Sumnapun, Norio Yamada, Patama Monkongdee, Amornrat Anuwatnonthakate, Channawong Burapat, Charles D Wells, Jordan W Tappero, Jay K Varma
{"title":"Multi-drug resistant TB and HIV in Thailand: overlapping, but not independently associated risk factors.","authors":"Somsak Akksilp, Wanpen Wattanaamornkiat, Wanitchaya Kittikraisak, Sriprapa Nateniyom, Somsak Rienthong, Chawin Sirinak, Keerataya Ngamlert, Wiroj Mankatittham, Wanchai Sattayawuthipong, Surin Sumnapun, Norio Yamada, Patama Monkongdee, Amornrat Anuwatnonthakate, Channawong Burapat, Charles D Wells, Jordan W Tappero, Jay K Varma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The HIV and multi-drug resistant tuberculosis (MDR-TB) epidemics are closely linked. In Thailand as part of a sentinel surveillance system, we collected data prospectively about pulmonary TB cases treated in public clinics. A subset of HIV-infected TB patients identified through this system had additional data collected for a research study. We conducted multivariate analysis to identify factors associated with MDR-TB. Of 10,428 TB patients, 2,376 (23%) were HIV-infected; 145 (1%) had MDR-TB. Of the MDR-TB cases, 52 (37%) were HIV-infected. Independent risk factors for MDR-TB included age 18-29 years old, male sex, and previous TB treatment, but not HIV infection. Among new patients, having an injection drug use history was a risk factor for MDR-TB. Of 539 HIV-infected TB patients in the research study, MDR-TB was diagnosed in 19 (4%); the only significant risk factors were previous TB treatment and previous hepatitis. In Thailand, HIV is common among MDR-TB patients, but is not an independent risk factor for MDR-TB. Populations at high risk for HIV-young adults, men, injection drug users - should be prioritized for drug susceptibility testing.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1264-78"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tove Heger, Saint Chin Han, Yang Van Heng, Mads Sundet, Stig Larsen, Hans Husums
{"title":"Early diagnosis and treatment of falciparum malaria in Cambodian trauma patients.","authors":"Tove Heger, Saint Chin Han, Yang Van Heng, Mads Sundet, Stig Larsen, Hans Husums","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Asymptomatically infected patients with falciparum malaria may develop symptomatic malaria infection secondary to injury or surgery. This complication increases the risk for postoperative wound infection and adds to the burden of trauma. The aims of the present study were to investigate the preventive effect of early antimalaria treatment of Plasmodium falciparum infected trauma patients, and to study the validity and accuracy of a rapid test to identify those infected. An open, non-randomized, interventional multi-center, cohort study was carried out at six district hospitals in northwestern Cambodia. Two hundred twenty-two trauma patients was examined for P. falciparum by dipstick test soon after injury. The patients testing positive were immediately treated with artesunate-mefloquine. A subset of 108 patients from Pailin, an area considered highly endemic for falciparum malaria, was used for the main analysis. Of 28 P. falciparum rapid test-positives, 21 developed symptomatic postinjury malaria despite early antimalarial treatment. The agreement between the dipstick test and blood smear examination was good (kappa 92.5; 95% CI 84.5-100). Early pre-operative treatment of parasite carriers does not seem to prevent symptomatic malaria after injury and surgery. The rapid test for falciparum malaria was reliable in early identification of asymptomatic P. falciparum infected patients.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1135-47"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dissemination of class I integron in Acinetobacter baumliannii isolated from ventilator-associated pneumonia patients and their environment.","authors":"Suntariya Sirichot, Pornphan Diraphat, Fuangfa Utrarachkij, Chanwit Tribuddharat, Kanokrat Siripanichgon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Multidrug resistant Acinetobacter baumannii has become the most common cause of health care-associated infections at Maharaj Nakhon Si Thammarat Hospital, Thailand. The objective of the study was to detect integrons using PCR-based method from 96 A. baumannii isolates from ventilator-associated pneumonia (VAP) patients and their environment. Antibiotic susceptibility was determined using a disk diffusion technique. Forty-six isolates exhibited integrase genes, with only class I and class II integron detected in 43 and 3 A. baumannii isolates, respectively. Twenty-seven of 52 clinical and 19 of 44 environmental isolates were integron-positive. Detection rate of integron-positive A. baumannii isolated from VAP patients increased from 25% to 83% over the 4 month study period. The majority (91%) of integron-positive A. baumannii showed resistance to 6 or more of 11 antibiotics tested and 72% of class I integron-positive isolates were imipenem-resistant. Thus, class I integron-positive A. baumannii had spread among the VAP patients and into hospital environment, the latter acting as reservoirs of potential pathogens possessing drug resistance genes.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1284-92"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azlihanis Abdul Hadi, Nyi Nyi Naing, Aziah Daud, Rusli Nordin, Mohd Rahim Sulong
{"title":"Prevalence and factors associated with stress among secondary school teachers in Kota Bharu, Kelantan, Malaysia.","authors":"Azlihanis Abdul Hadi, Nyi Nyi Naing, Aziah Daud, Rusli Nordin, Mohd Rahim Sulong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The teaching profession is an occupation at high risk for stress. This research attempted to determine the prevalence of stress and the associated factors contributing to stress among teachers in Malaysia. A cross-sectional study was conducted on 580 secondary school teachers in Kota Bharu District. The instrument used to carry out the study was adopted and modified from the Depression, Anxiety and Stress Scale (DASS 21) and Job Content Questionnaire (JCQ). The questionnaire consisted of two parts: Part I consisting non-job factors (socio-demographic characteristics) and Part II consisting of psychosocial factors contributing to stress. Simple and multiple linear regression analysis were carried out. The prevalence of stress was reported as 34.0%. Seventeen point four percent of teachers experienced mild stress. Age, duration of work and psychological job demands were significantly associated with stress level. This study indicates job-related factors did not contribute much to stress among secondary school teachers. Non-job-related factors should be further studied to determine methods for stress reduction in teachers in Malaysia.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1359-70"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T M Roshan, H Rosline, S A Ahmed, M Rapiaah, M N Khattak
{"title":"Response rate of Malaysian blood donors with reactive screening test to transfusion medicine unit calls.","authors":"T M Roshan, H Rosline, S A Ahmed, M Rapiaah, M N Khattak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood donors with reactive screening test results are requested to come in for counseling by letter and telephone call. It has been noticed many donors responded to neither the letters nor the telephone calls. We evaluated 589 cases with reactive screening test results (208 positive for hepatitis C, 209 for hepatitis B, 85 for VDRL and 87 for HIV). In the hepatitis C positive group 61 donors (29.3%) did not respond and 4.7% missed their follow-up appointment. Similarly low response rates were noted with the HBV (58.9%) and VDRL (67.1%) positive groups. Among HIV positive donors 46.0% failed to respond to multiple calls. We conclude that blood donors in Malaysia have a poor response to calls from the blood transfusion unit. A review of the effectiveness of the current deferral system and an increased public knowledge of transmissible infectious diseases may encourage blood donors to have a better response rate.</p>","PeriodicalId":520816,"journal":{"name":"The Southeast Asian journal of tropical medicine and public health","volume":" ","pages":"1315-21"},"PeriodicalIF":0.2,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29080969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}