Rice (New York, N.Y.)最新文献

{"title":"The PPR-Domain Protein SOAR1 Regulates Salt Tolerance in Rice.","authors":"Kai Lu,&nbsp;Cheng Li,&nbsp;Ju Guan,&nbsp;Wen-Hua Liang,&nbsp;Tao Chen,&nbsp;Qing-Yong Zhao,&nbsp;Zhen Zhu,&nbsp;Shu Yao,&nbsp;Lei He,&nbsp;Xiao-Dong Wei,&nbsp;Ling Zhao,&nbsp;Li-Hui Zhou,&nbsp;Chun-Fang Zhao,&nbsp;Cai-Lin Wang,&nbsp;Ya-Dong Zhang","doi":"10.1186/s12284-022-00608-x","DOIUrl":"https://doi.org/10.1186/s12284-022-00608-x","url":null,"abstract":"<p><p>Previous studies in Arabidopsis reported that the PPR protein SOAR1 plays critical roles in plant response to salt stress. In this study, we reported that expression of the Arabidopsis SOAR1 (AtSOAR1) in rice significantly enhanced salt tolerance at seedling growth stage and promoted grain productivity under salt stress without affecting plant productivity under non-stressful conditions. The transgenic rice lines expressing AtSOAR1 exhibited increased ABA sensitivity in ABA-induced inhibition of seedling growth, and showed altered transcription and splicing of numerous genes associated with salt stress, which may explain salt tolerance of the transgenic plants. Further, we overexpressed the homologous gene of SOAR1 in rice, OsSOAR1, and showed that transgenic plants overexpressing OsSOAR1 enhanced salt tolerance at seedling growth stage. Five salt- and other abiotic stress-induced SOAR1-like PPRs were also identified. These data showed that the SOAR1-like PPR proteins are positively involved in plant response to salt stress and may be used for crop improvement in rice under salinity conditions through transgenic manipulation.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"62"},"PeriodicalIF":5.5,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40458888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Domestication Loci Associated with Awn Development in Rice.","authors":"Ngoc Ha Luong,&nbsp;Sangshetty G Balkunde,&nbsp;Kyu-Chan Shim,&nbsp;Cheryl Adeva,&nbsp;Hyun-Sook Lee,&nbsp;Hyun-Jung Kim,&nbsp;Sang-Nag Ahn","doi":"10.1186/s12284-022-00607-y","DOIUrl":"https://doi.org/10.1186/s12284-022-00607-y","url":null,"abstract":"<p><p>Rice (Oryza sativa L.) is a widely studied domesticated model plant. Seed awning is an unfavorable trait during rice harvesting and processing. Hence, loss of awn was one of the target characters selected during domestication. However, the genetic mechanisms underlying awn development in rice are not well understood. In this study, we analyzed and characterized the genes for awn development using a mapping population derived from a cross between the Korean indica cultivar 'Milyang23' and a near-isogenic line NIL4/9 derived from a cross between 'Hwaseong' and Oryza minuta. Two quantitative trait loci (QTLs), qAwn4 and qAwn9, mapped on chromosomes 4 and 9, respectively, increased awn length in an additive manner. Through comparative sequencing analyses of the parental lines, LABA1 was determined as the causal gene underlying qAwn4. qAwn9 was mapped to a 199-kb physical region between markers RM24663 and RM24679. Within this interval, 27 annotated genes were identified, and five genes, including a basic leucine zipper transcription factor 76 (OsbZIP76), were considered as candidate genes for qAwn9 based on their functional annotations and sequence variations. Haplotype analysis using the candidate gene revealed tropical-japonica specific sequence variants in the qAwn9 region, which partly explains the non-detection of qAwn9 in previous studies that used progenies from interspecific crosses. This provides further evidence that OsbZIP76 is possibly a causal gene for qAwn9. The O. minuta qAwn9 allele was identified as a major QTL, providing an important molecular target for understanding the genetic control of awn development in rice. Our results lay the foundation for further cloning of the awn gene underlying qAwn9.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"61"},"PeriodicalIF":5.5,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40712375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL Detection for Rice Grain Length and Fine Mapping of a Novel Locus qGL6.1.","authors":"Mingzhu Zhao,&nbsp;Yuanzheng Wang,&nbsp;Na He,&nbsp;Xiu Pang,&nbsp;Lili Wang,&nbsp;Zuobin Ma,&nbsp;Zhiqiang Tang,&nbsp;Hong Gao,&nbsp;Liying Zhang,&nbsp;Liang Fu,&nbsp;Changhua Wang,&nbsp;Jingang Liu,&nbsp;Wenjing Zheng","doi":"10.1186/s12284-022-00606-z","DOIUrl":"https://doi.org/10.1186/s12284-022-00606-z","url":null,"abstract":"<p><strong>Background: </strong>Grain length (GL) that is directly associated with appearance quality is a key target of selection in rice breeding. Although abundant quantitative trait locus (QTL) associated with GL have been identified, it was still relatively weak to identify QTL for GL from japonica genetic background, as the shortage of japonica germplasms with long grains. We performed QTLs analysis for GL using a recombinant inbred lines (RILs) population derived from the cross between japonica variety GY8 (short grains) and LX1 (long grains) in four environments.</p><p><strong>Results: </strong>A total of 197 RILs were genotyped with 285 polymorphic SNP markers. Three QTLs qGL5.3, qGL6.1 and qGL11 were detected to control GL by individual environmental analyses and multi-environment joint analysis. Of these, a major-effect and stable QTL qGL6.1 was identified to be a novel QTL, and its LX1 allele had a positive effect on GL. For fine-mapping qGL6.1, a BC₁F₂ population consisting of 2,487 individuals was developed from a backcross between GY8 and R176, one line with long grain. Eight key informative recombinants were identified by nine kompetitive allele specific PCR (KASP) markers. By analyzing key recombinants, the qGL6.1 locus was narrowed down to a 40.41 kb genomic interval on chromosome 6. One candidate gene LOC_Os06g43304.1 encoding cytochrome P450 (CYP71D55) was finally selected based on the difference in the transcriptional expression and variations in its upstream and downstream region.</p><p><strong>Conclusions: </strong>Three QTLs qGL5.3, qGL6.1 and qGL11 were identified to control grain length in rice. One novel QTL qGL6.1 was fine mapped within 40.41 kb region, and LOC_Os06g43304.1 encoding cytochrome P450 (CYP71D55) may be its candidate gene. We propose that the further cloning of the qGL6.1 will facilitate improving appearance quality in japonica varieties.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"60"},"PeriodicalIF":5.5,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40490096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice.","authors":"Weilan Chen,&nbsp;Xiaoling Hu,&nbsp;Li Hu,&nbsp;Xinyue Hou,&nbsp;Zhengyan Xu,&nbsp;Fanmin Yang,&nbsp;Min Yuan,&nbsp;Feifan Chen,&nbsp;Yunxiao Wang,&nbsp;Bin Tu,&nbsp;Ting Li,&nbsp;Liangzhu Kang,&nbsp;Shiwen Tang,&nbsp;Bingtian Ma,&nbsp;Yuping Wang,&nbsp;Shigui Li,&nbsp;Peng Qin,&nbsp;Hua Yuan","doi":"10.1186/s12284-022-00601-4","DOIUrl":"https://doi.org/10.1186/s12284-022-00601-4","url":null,"abstract":"<p><strong>Background: </strong>Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown.</p><p><strong>Findings: </strong>We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants.</p><p><strong>Conclusion: </strong>WG3 functions directly in regulating grain size and BR signaling in rice.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"55"},"PeriodicalIF":5.5,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40444637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy.","authors":"Kai Sun,&nbsp;Dandan Li,&nbsp;Aoyun Xia,&nbsp;Hua Zhao,&nbsp;Qin Wen,&nbsp;Sisi Jia,&nbsp;Jiafeng Wang,&nbsp;Guili Yang,&nbsp;Danhua Zhou,&nbsp;Cuihong Huang,&nbsp;Hui Wang,&nbsp;Zhiqiang Chen,&nbsp;Tao Guo","doi":"10.1186/s12284-022-00603-2","DOIUrl":"https://doi.org/10.1186/s12284-022-00603-2","url":null,"abstract":"<p><strong>Background: </strong>The mining of new allelic variation and the induction of new genetic variability are the basis for improving breeding efficiency.</p><p><strong>Results: </strong>In this study, in total, 3872 heavy ion-irradiated M₂ generation rice seeds and individual leaves were collected. The grain length was between 8 and 10.22 mm. The grain width was between 1.54 and 2.87 mm. The results showed that there was extensive variation in granulotype. The allelic variation in GS3 and GW5 was detected in 484 mixed samples (8:1) using targeted sequencing technology, and 12 mixed samples containing potential mutations and 15 SNPs were obtained; combined with Sanger sequencing and phenotype data, 13 key mutants and their corresponding SNPs were obtained; protein structural and functional analysis of key mutants screened out 6 allelic variants leading to altered grain shape, as well as the corresponding mutants, including long-grain mutants GS3-2 and GS3-7, short-grain mutants GS3-3 and GS3-5, wide-grain mutant GW5-1 and narrow-grain mutant GW5-4; whole genome sequencing identified new grain length gene allelic variants GS3-G1, GS3-G2 and GS3-G3.</p><p><strong>Conclusion: </strong>Based on the above studies, we found 6 granulotype mutants and 9 granulotype-related allelic variants, which provided new functional gene loci and a material basis for molecular breeding and genotype mutation and phenotype analysis. We propose a method for targeted identification of allelic variation in rice grain type genes by combining targeted sequencing of mixed samples and whole genome sequencing. The method has the characteristics of low detection cost, short detection period, and flexible detection of traits and genes.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"57"},"PeriodicalIF":5.5,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40452259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variety-Specific Transcriptional and Alternative Splicing Regulations Modulate Salt Tolerance in Rice from Early Stage of Stress.","authors":"Guihua Jian,&nbsp;Yujian Mo,&nbsp;Yan Hu,&nbsp;Yongxiang Huang,&nbsp;Lei Ren,&nbsp;Yueqin Zhang,&nbsp;Hanqiao Hu,&nbsp;Shuangxi Zhou,&nbsp;Gang Liu,&nbsp;Jianfu Guo,&nbsp;Yu Ling","doi":"10.1186/s12284-022-00599-9","DOIUrl":"https://doi.org/10.1186/s12284-022-00599-9","url":null,"abstract":"<p><p>Salt stress poses physiological drought, ionic toxicity and oxidative stress to plants, which causes premature senescence and death of the leaves if the stress sustained. Salt tolerance varied between different rice varieties, but how different rice varieties respond at the early stage of salt stress has been seldom studied comprehensively. By employing third generation sequencing technology, we compared gene expressional changes in leaves of three rice varieties that varied in their level of tolerance after salt stress treatment for 6 h. Commonly up-regulated genes in all rice varieties were related to water shortage response and carbon and amino acids metabolism at the early stage of salt stress, while reactive oxygen species cleavage genes were induced more in salt-tolerant rice. Unexpectedly, genes involved in chloroplast development and photosynthesis were more significantly down-regulated in the two salt tolerant rice varieties 'C34' and 'Nona Bokra'. At the same time, genes coding ribosomal protein were suppressed to a more severe extent in the salt-sensitive rice variety 'IR29'. Interestingly, not only variety-specific gene transcriptional regulation, but also variety-specific mRNA alternative splicing, on both coding and long-noncoding genes, were found at the early stage of salt stress. In summary, differential regulation in gene expression at both transcriptional and post-transcriptional levels, determine and fine-tune the observed response in level of damage in leaves of specific rice genotypes at early stage of salt stress.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"56"},"PeriodicalIF":5.5,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40452257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Enhancement of iron Deficiency Tolerance and Iron Accumulation in Rice by Combining the Knockdown of OsHRZ Ubiquitin Ligases with the Introduction of Engineered Ferric-chelate Reductase.","authors":"Takanori Kobayashi,&nbsp;Keisuke Maeda,&nbsp;Yutaro Suzuki,&nbsp;Naoko K Nishizawa","doi":"10.1186/s12284-022-00598-w","DOIUrl":"https://doi.org/10.1186/s12284-022-00598-w","url":null,"abstract":"<p><p>Iron is an essential micronutrient for living organisms, but its solubility is extremely low under alkaline conditions. Plants often suffer from iron deficiency chlorosis in calcareous soils, which consist of approximately 30% of the world's cultivated area, severely limiting plant productivity. Iron deficiency anemia is also a widespread problem in humans, especially in Asian and African people who take up iron mainly from staple foods containing low iron concentrations. Transgenic manipulation of genes involved in plant iron uptake, translocation, and storage has made improvements in enhancing iron deficiency tolerance or iron accumulation in edible parts, but these two properties have been characterized separately. We previously produced transgenic rice lines, with concomitant improvement of iron deficiency tolerance and grain iron accumulation by knocking-down OsHRZ ubiquitin ligases, which negatively regulate iron deficiency response and iron accumulation in rice. In the present report, we aimed to further improve the iron deficiency tolerance and grain iron accumulation of OsHRZ knockdown rice by the simultaneous introduction of the engineered ferric-chelate reductase gene Refre1/372 under the control of the OsIRT1 promoter for further enhancement of iron uptake. We obtained several transgenic rice lines with repressed OsHRZ expression and induced Refre1/372 expression. These lines showed a variable degree of iron deficiency tolerance in calcareous soils, with increased iron accumulation in brown seeds under both iron-deficient and iron-sufficient soil cultures. Selected OsHRZ knockdown plus Refre1/372 lines showed similar or better growth compared with that of singly introduced OsHRZ knockdown or Refre1/372 lines in calcareous soils under both non-submerged and submerged conditions. After submerged calcareous soil cultivation, these OsHRZ knockdown plus Refre1/372 lines accumulated 2.5-4.3 times and 17-23 times more iron concentrations than that of non-transformants in brown rice and straw, respectively, which was comparable or superior to a single OsHRZ knockdown line. Our results indicate that the combined introduction of OsHRZ knockdown and OsIRT1 promoter-Refre1/372 is highly effective in further improving the iron deficiency tolerance without compromising the iron accumulation of the OsHRZ knockdown effects.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"54"},"PeriodicalIF":5.5,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9622965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40659463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile Organic Compounds, Evaluation Methods and Processing Properties for Cooked Rice Flavor.","authors":"Zichen Zheng,&nbsp;Chao Zhang,&nbsp;Kewei Liu,&nbsp;Qiaoquan Liu","doi":"10.1186/s12284-022-00602-3","DOIUrl":"https://doi.org/10.1186/s12284-022-00602-3","url":null,"abstract":"<p><p>Rice (Oryza sativa L.), as the main refined grain in China, has attracted much attention in terms of quality. Rice is usually consumed after cooking, and it is a commonly staple food. Nowdays, people's requirements for cooked rice focus more on the taste characteristics and quality. Furthermore, aroma is one of the primary sensory reference points, which is the most intuitive way for people to judge cooked rice. By integrating and analyzing the researches of cooked rice aroma identification in recent five years, this paper expounds the extraction and identification methods (sensory evaluation method, GC-MS, SPME, MOS sensors, electronic nose, etc.) of the flavor substances in cooked rice, as the processing methods and properties of cooked rice, and the volatile organic compounds of cooked rice under different conditions are summarized as well.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"53"},"PeriodicalIF":5.5,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40456582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping of Candidate Genes in Response to Low Nitrogen in Rice Seedlings.","authors":"Jia Li,&nbsp;Wei Xin,&nbsp;Weiping Wang,&nbsp;Shijiao Zhao,&nbsp;Lu Xu,&nbsp;Xingdong Jiang,&nbsp;Yuxuan Duan,&nbsp;Hongliang Zheng,&nbsp;Luomiao Yang,&nbsp;Hualong Liu,&nbsp;Yan Jia,&nbsp;Detang Zou,&nbsp;Jingguo Wang","doi":"10.1186/s12284-022-00597-x","DOIUrl":"https://doi.org/10.1186/s12284-022-00597-x","url":null,"abstract":"<p><p>Nitrogen is not only a macronutrient essential for crop growth and development, but also one of the most critical nutrients in farmland ecosystem. Insufficient nitrogen supply will lead to crop yield reduction, while excessive application of nitrogen fertilizer will cause agricultural and eco-environment damage. Therefore, mining low-nitrogen tolerant rice genes and improving nitrogen use efficiency are of great significance to the sustainable development of agriculture. This study was conducted by Genome-wide association study on a basis of two root morphological traits (root length and root diameter) and 788,396 SNPs of a natural population of 295 rice varieties. The transcriptome of low-nitrogen tolerant variety (Longjing 31) and low-nitrogen sensitive variety (Songjing 10) were sequenced between low and high nitrogen treatments. A total of 35 QTLs containing 493 genes were mapped. 3085 differential expressed genes were identified. Among these 493 genes, 174 genes showed different haplotype patterns. There were significant phenotype differences among different haplotypes of 58 genes with haplotype differences. These 58 genes were hypothesized as candidate genes for low nitrogen tolerance related to root morphology. Finally, six genes (Os07g0471300, Os11g0230400, Os11g0229300, Os11g0229400, Os11g0618300 and Os11g0229333) which expressed differentially in Longjing 31 were defined as more valuable candidate genes for low-nitrogen tolerance. The results revealed the response characteristics of rice to low-nitrogen, and provided insights into regulatory mechanisms of rice to nitrogen deficiency.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"51"},"PeriodicalIF":5.5,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33512538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsWHY1 Interacts with OsTRX z and is Essential for Early Chloroplast Development in Rice.","authors":"Zhennan Qiu,&nbsp;Dongdong Chen,&nbsp;Linhong Teng,&nbsp;Peiyan Guan,&nbsp;Guoping Yu,&nbsp;Peiliang Zhang,&nbsp;Jian Song,&nbsp;Qiangcheng Zeng,&nbsp;Li Zhu","doi":"10.1186/s12284-022-00596-y","DOIUrl":"https://doi.org/10.1186/s12284-022-00596-y","url":null,"abstract":"<p><p>WHIRLY (WHY) family proteins, a small family of single-stranded DNA (ssDNA) binding proteins, are widely found in plants and have multiple functions to regulate plant growth and development. However, WHY in rice has received less attention. In this study, we continued our previous study on OsTRX z that is important for chloroplast development. OsTRX z was discovered to interact with OsWHY1, which was confirmed using yeast two-hybrid, pull-down, and BiFC assays. Subsequently, the oswhy1 mutants were obtained by CRISPR/Cas9, which exhibited an albino phenotype and died after the three-leaf stage. Consistent with this albino phenotype, low amounts of Chl a, Chl b, and Car were detected in the oswhy1-1 mutant. Moreover, the oswhy1-1 mutant had chloroplasts with disrupted architecture and no stacked grana and thylakoid membranes. Subcellular localization showed that the OsWHY1-GFP fusion protein was targeted to the chloroplast. What's more, OsWHY1 was found to be preferentially expressed in young leaves and was involved in chloroplast RNA editing and splicing. Mutation of OsWHY1 significantly affected the expression of chloroplast and ribosome development-related and chlorophyll synthesis-related genes. In conclusion, OsWHY1 contributes to early chloroplast development and normal seedling survival in rice. These results will further elucidate the molecular mechanism of chloroplast development and expand our understanding of WHY1 functions.</p>","PeriodicalId":520771,"journal":{"name":"Rice (New York, N.Y.)","volume":" ","pages":"50"},"PeriodicalIF":5.5,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33495240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}