Research (Washington, D.C.)最新文献

{"title":"Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion.","authors":"Haijiao Xu,&nbsp;Jinrui Zhang,&nbsp;Yijia Zhou,&nbsp;Guanfang Zhao,&nbsp;Mingjun Cai,&nbsp;Jing Gao,&nbsp;Lina Shao,&nbsp;Yan Shi,&nbsp;Hongru Li,&nbsp;Hongbin Ji,&nbsp;Yikai Zhao,&nbsp;Hongda Wang","doi":"10.34133/2022/9835035","DOIUrl":"https://doi.org/10.34133/2022/9835035","url":null,"abstract":"<p><p>Most plasmalemmal proteins are organized into clusters to modulate various cellular functions. However, the machineries that regulate protein clustering remain largely unclear. Here, with EGFR as an example, we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane (PM) using a high-speed, high-resolution spinning-disk confocal microscope. First, colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM, eventually forming different distribution forms on the apical and basal membranes; that is, most EGFR formed larger clusters on the apical membrane than the basal membrane. A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters, and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell proliferation. Finally, we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the PM. More importantly, we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the PM. Collectively, a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM, highlighting the correlation between EGFR secretion and its PM distribution pattern.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9835035"},"PeriodicalIF":11.0,"publicationDate":"2022-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9620640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40669086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facet Engineering in Constructing Lewis Acid-Base Pairs for CO₂ Cycloaddition to High Value-Added Carbonates.","authors":"Shu Shang,&nbsp;Wei Shao,&nbsp;Xiao Luo,&nbsp;Ming Zuo,&nbsp;Hui Wang,&nbsp;Xiaodong Zhang,&nbsp;Yi Xie","doi":"10.34133/2022/9878054","DOIUrl":"https://doi.org/10.34133/2022/9878054","url":null,"abstract":"<p><p>Cycloaddition of epoxides with CO₂ to synthesis cyclic carbonates is an atom-economic pathway for CO₂ utilization with promising industry application value, while its efficiency was greatly inhibited for the lack of highly active catalytic sites. Herein, by taking BiOX (X = Cl, Br) with layered structure for example, we proposed a facet engineering strategy to construct Lewis acid-base pairs for CO₂ cycloaddition, where the typical BiOBr with (010) facets expose surface Lewis acid Bi sites and Lewis base Br sites simultaneously. By the combination of <i>in-situ</i> diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and theoretical calculations, the oxygen atom of the epoxide is interacted with the Lewis acid Bi site to activate the ternary ring, then facilitates the attack of the carbon atom by the Lewis base Br site for the ring-opening of the epoxide, which is the rate-determining step in the cycloaddition reaction. As a result, the BiOBr-(010) with rich surface Lewis acid-base pairs showed a high conversion of 85% with 100% atomic economy in the synthesis of cyclic-carbonates without any cocatalyst. This study provides a model structure for CO₂ cycloaddition to high value-added long chain chemicals.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9878054"},"PeriodicalIF":11.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40661475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the Defect-Dominated Electron Scattering in Mg₃Sb₂-Based Thermoelectric Materials.","authors":"Jucai Jia,&nbsp;Yan Zhou,&nbsp;Xiaoxi Chen,&nbsp;Wenhua Xue,&nbsp;Hulei Yu,&nbsp;Jing Li,&nbsp;Shizhen Zhi,&nbsp;Chen Chen,&nbsp;Jian Wang,&nbsp;Shuaihang Hou,&nbsp;Xingjun Liu,&nbsp;Yumei Wang,&nbsp;Feng Cao,&nbsp;Yue Chen,&nbsp;Jun Mao,&nbsp;Qian Zhang","doi":"10.34133/2022/9875329","DOIUrl":"https://doi.org/10.34133/2022/9875329","url":null,"abstract":"<p><p>The thermoelectric parameters are essentially governed by electron and phonon transport. Since the carrier scattering mechanism plays a decisive role in electron transport, it is of great significance for the electrical properties of thermoelectric materials. As a typical example, the defect-dominated carrier scattering mechanism can significantly impact the room-temperature electron mobility of n-type Mg₃Sb₂-based materials. However, the origin of such a defect scattering mechanism is still controversial. Herein, the existence of the Mg vacancies and Mg interstitials has been identified by synchrotron powder X-ray diffraction. The relationship among the point defects, chemical compositions, and synthesis conditions in Mg₃Sb₂-based materials has been revealed. By further introducing the point defects without affecting the grain size via neutron irradiation, the thermally activated electrical conductivity can be reproduced. Our results demonstrate that the point defects scattering of electrons is important in the n-type Mg₃Sb₂-based materials.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9875329"},"PeriodicalIF":11.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9620639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40670029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-Depth Metaproteomics Analysis of Oral Microbiome for Lung Cancer.","authors":"Xiaoteng Jiang,&nbsp;Yan Zhang,&nbsp;Huiyu Wang,&nbsp;Zeyuan Wang,&nbsp;Shen Hu,&nbsp;Chengxi Cao,&nbsp;Hua Xiao","doi":"10.34133/2022/9781578","DOIUrl":"https://doi.org/10.34133/2022/9781578","url":null,"abstract":"<p><p>The human oral microbiome correlates with numerous diseases, including lung cancer. Identifying the functional changes by metaproteomics helps understand the disease-related dysbiosis, yet characterizing low-abundant bacteria is challenging. Here, we developed a free-flow isoelectric focusing electrophoresis-mass spectrometry- (FFIEF-MS-) based metaproteomics strategy to reduce host interferences and enrich low-abundant bacteria for in-depth interpretation of the oral microbiome. With our method, the number of interfering peptides decreased by 52.87%, whereas the bacterial peptides and species increased by 94.97% and 44.90%, respectively, compared to the conventional metaproteomics approach. We identified 3647 bacterial proteins, which is the most comprehensive oral metaproteomics study to date. Lung cancer-associated bacteria were validated among an independent cohort. The imbalanced <i>Fusobacterium nucleatum</i> and <i>Prevotella histicola</i> and their dysregulated functions in inhibiting immune response and maintaining cell redox homeostasis were revealed. The FFIEF-MS may serve as a valuable strategy to study the mechanisms between human diseases and microbiomes with broader applications.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9781578"},"PeriodicalIF":11.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40661474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low Trap Density Para-F Substituted 2D PEA₂PbX₄ (X = Cl, Br, I) Single Crystals with Tunable Optoelectrical Properties and High Sensitive X-Ray Detector Performance.","authors":"Jiayu Di,&nbsp;Haojin Li,&nbsp;Li Chen,&nbsp;Siyu Zhang,&nbsp;Yinhui Hu,&nbsp;Kai Sun,&nbsp;Bo Peng,&nbsp;Jie Su,&nbsp;Xue Zhao,&nbsp;Yuqi Fan,&nbsp;Zhenhua Lin,&nbsp;Yue Hao,&nbsp;Peng Gao,&nbsp;Kui Zhao,&nbsp;Jingjing Chang","doi":"10.34133/2022/9768019","DOIUrl":"https://doi.org/10.34133/2022/9768019","url":null,"abstract":"<p><p>Exploring halogen engineering is of great significance for reducing the density of defect states in crystals of organic-inorganic hybrid perovskites and hence improving the crystal quality. Herein, high-quality single crystals of PEA₂PbX₄ (X = Cl, Br, I) and their para-F (<i>p</i>-F) substitution analogs are prepared using the facile solution method to study the effects of both <i>p</i>-F substitution and halogen anion engineering. After <i>p</i>-F substitution, the triclinic PEA₂PbX₄ (X = Cl, Br) and cubic PEA₂PbX₄ (X = I) crystals unifies to monoclinic crystal structure for <i>p</i>-F-PEA₂PbX₄ (X = Cl, Br, I) crystals. The <i>p</i>-F substitution and halogen engineering, together with crystal structure variation, enable the tunability of optoelectrical properties. Experimentally, after the <i>p</i>-F substitution, the energy levels are lowered with increased Fermi levels, and the bandgaps of <i>p</i>-F-PEA₂PbX₄ (X = Cl, Br, I) are slightly reduced. Benefitting from the enhancement of the charge transfer and the reduced trap density by <i>p</i>-F substitution and halogen anion engineering, the average carrier lifetime of the <i>p</i>-F-PEA₂PbX₄ is obviously reduced. Compared with PEA₂PbI₄, the X-ray detector based on <i>p</i>-F-PEA₂PbI₄ perovskite single-crystal has a higher sensitivity of 119.79 <i>μ</i>C Gy_air ^-1·cm^-2. Moreover, the X-ray detector based on <i>p</i>-F-PEA₂PbI₄ single crystals exhibits higher radiation stability under high-dose X-ray irradiation, implying long-term operando stability.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9768019"},"PeriodicalIF":11.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40661472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding.","authors":"Lexun Wang,&nbsp;Jiaojiao Feng,&nbsp;Yanyue Deng,&nbsp;Qianqian Yang,&nbsp;Quxing Wei,&nbsp;Dewei Ye,&nbsp;Xianglu Rong,&nbsp;Jiao Guo","doi":"10.34133/2022/9891689","DOIUrl":"https://doi.org/10.34133/2022/9891689","url":null,"abstract":"<p><p>CCAAT/enhancer-binding proteins (C/EBPs) are a family of at least six identified transcription factors that contain a highly conserved basic leucine zipper domain and interact selectively with duplex DNA to regulate target gene expression. C/EBPs play important roles in various physiological processes, and their abnormal function can lead to various diseases. Recently, accumulating evidence has demonstrated that aberrant C/EBP expression or activity is closely associated with the onset and progression of fibrosis in several organs and tissues. During fibrosis, various C/EBPs can exert distinct functions in the same organ, while the same C/EBP can exert distinct functions in different organs. Modulating C/EBP expression or activity could regulate various molecular processes to alleviate fibrosis in multiple organs; therefore, novel C/EBPs-based therapeutic methods for treating fibrosis have attracted considerable attention. In this review, we will explore the features of C/EBPs and their critical functions in fibrosis in order to highlight new avenues for the development of novel therapies targeting C/EBPs.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9891689"},"PeriodicalIF":11.0,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40431040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding a Single-Li-Ion COF Conductor for Being Dendrite Free in a Li-Organic Battery.","authors":"Yongjiang Sun,&nbsp;Genfu Zhao,&nbsp;Yao Fu,&nbsp;Yongxin Yang,&nbsp;Conghui Zhang,&nbsp;Qi An,&nbsp;Hong Guo","doi":"10.34133/2022/9798582","DOIUrl":"https://doi.org/10.34133/2022/9798582","url":null,"abstract":"<p><p>In addition to improving ion conductivity and the transference number, single-Li-ion conductors (SLCs) also enable the elimination of interfacial side reactions and concentration difference polarization. Therefore, the SLCs can achieve high performance in solid-state batteries with Li metal as anode and organic molecule as cathode. Covalent organic frameworks (COFs) are leading candidates for constructing SLCs because of the excellent 1D channels and accurate chemical-modification skeleton. Herein, various contents of lithium-sulfonated covalently anchored COFs (denoted as LiO₃S-COF1 and LiO₃S-COF2) are controllably synthesized as SLCs. Due to the directional ion channels, high Li contents, and single-ion frameworks, LiO₃S-COF2 shows exceptional Li-ion conductivity of 5.47 × 10^-5 S · cm^-1, high transference number of 0.93, and low activation energy of 0.15 eV at room temperature. Such preeminent Li-ion-transported properties of LiO₃S-COF2 permit stable Li⁺ plating/stripping in a symmetric lithium metal battery, effectively impeding the Li dendrite growth in a liquid cell. Moreover, the designed quasi-solid-state cell (organic anthraquinone (AQ) as cathode, Li metal as anode, and LiO₃S-COF2 as electrolyte) shows high-capacity retention and rate behavior. Consequently, LiO₃S-COF2 implies a potential value restraining the dissolution of small organic molecules and Li dendrite growth.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9798582"},"PeriodicalIF":11.0,"publicationDate":"2022-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40427526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Muscle-Tendon Progenitor Subpopulation in Human Myotendinous Junction at Single-Cell Resolution.","authors":"Ruojin Yan,&nbsp;Hong Zhang,&nbsp;Yuanzhu Ma,&nbsp;Ruifu Lin,&nbsp;Bo Zhou,&nbsp;Tao Zhang,&nbsp;Chunmei Fan,&nbsp;Yuxiang Zhang,&nbsp;Zetao Wang,&nbsp;Tianshun Fang,&nbsp;Zi Yin,&nbsp;Youzhi Cai,&nbsp;Hongwei Ouyang,&nbsp;Xiao Chen","doi":"10.34133/2022/9760390","DOIUrl":"https://doi.org/10.34133/2022/9760390","url":null,"abstract":"<p><p>The myotendinous junction (MTJ) is a complex and special anatomical area that connects muscles and tendons, and it is also the key to repairing tendons. Nevertheless, the anatomical structure and connection structure of MTJ, the cluster and distribution of cells, and which cells are involved in repairing the tissue are still unclear. Here, we analyzed the cell subtype distribution and function of human MTJ at single-cell level. We identified four main subtypes, including stem cell, muscle, tendon, and muscle-tendon progenitor cells (MTP). The MTP subpopulation, which remains the characteristics of stem cells and also expresses muscle and tendon marker genes simultaneously, may have the potential for bidirectional differentiation. We also found the muscle-tendon progenitor cells were distributed in the shape of a transparent goblet; muscle cells first connect to the MTP and then to the tendon. And after being transplanted in the MTJ injury model, MTP exhibited strong regenerative capability. Finally, we also demonstrated the importance of mTOR signaling for MTP maintenance by <i>in vitro</i> addition of rapamycin and <i>in vivo</i> validation using mTOR-ko mice. Our research conducted a comprehensive analysis of the heterogeneity of myotendinous junction, discovered a special cluster called MTP, provided new insights into the biological significance of myotendinous junction, and laid the foundation for future research on myotendinous junction regeneration and restoration.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9760390"},"PeriodicalIF":11.0,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40561796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whether and When Superhydrophobic/Superoleophobic Surfaces Are Fingerprint Repellent.","authors":"Chengjiao Wu,&nbsp;Yue Fan,&nbsp;Hongxin Wang,&nbsp;Juan Li,&nbsp;Yuxi Chen,&nbsp;Yingke Wang,&nbsp;Lin Liu,&nbsp;Lidan Zhou,&nbsp;Shilin Huang,&nbsp;Xuelin Tian","doi":"10.34133/2022/9850316","DOIUrl":"https://doi.org/10.34133/2022/9850316","url":null,"abstract":"<p><p>Driven by the ever-increasing demand for fingerprint-resistant techniques in modern society, numerous researches have proposed to develop innovative antifingerprint coatings based on superhydrophobic/superoleophobic surface design. However, whether superhydrophobic/superoleophobic surfaces have favorable repellency to the microscopic fingerprint is in fact an open question. Here, we establish a reliable method that enables evaluating the antifingerprint capability of various surfaces in a quantitative way. We show that superhydrophobicity is irrelevant with fingerprint repellency. Regarding superoleophobic surfaces, two distinct wetting states of microscopic fingerprint residues, i.e., the \"repellent\" and the \"collapsed\" states, are revealed. Only in the \"repellent\" state, in which the fingerprint residues remain atop surface textures upon being pressed, superoleophobic surfaces can bring about favorable antifingerprint repellency, which correlates positively with their receding contact angles. A finger-deformation-dependent intrusion mechanism is proposed to account for the formation of different fingerprint wetting states. Our findings offer important insights into the mechanism of fingerprint repellency and will help the design of high-performance antifingerprint surfaces for diverse applications.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9850316"},"PeriodicalIF":11.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40337661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared Core Microbiome and Functionality of Key Taxa Suppressive to Banana Fusarium Wilt.","authors":"Zongzhuan Shen,&nbsp;Linda S Thomashow,&nbsp;Yannan Ou,&nbsp;Chengyuan Tao,&nbsp;Jiabao Wang,&nbsp;Wu Xiong,&nbsp;Hongjun Liu,&nbsp;Rong Li,&nbsp;Qirong Shen,&nbsp;George A Kowalchuk","doi":"10.34133/2022/9818073","DOIUrl":"https://doi.org/10.34133/2022/9818073","url":null,"abstract":"<p><p>Microbial contributions to natural soil suppressiveness have been reported for a range of plant pathogens and cropping systems. To disentangle the mechanisms underlying suppression of banana Panama disease caused by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> tropical race 4 (Foc4), we used amplicon sequencing to analyze the composition of the soil microbiome from six separate locations, each comprised of paired orchards, one potentially suppressive and one conducive to the disease. Functional potentials of the microbiomes from one site were further examined by shotgun metagenomic sequencing after soil suppressiveness was confirmed by greenhouse experiments. Potential key antagonists involved in disease suppression were also isolated, and their activities were validated by a combination of microcosm and pot experiments. We found that potentially suppressive soils shared a common core community with relatively low levels of <i>F. oxysporum</i> and relatively high proportions of Myxococcales, Pseudomonadales, and Xanthomonadales, with five genera, <i>Anaeromyxobacter</i>, <i>Kofleria</i>, <i>Plesiocystis</i>, <i>Pseudomonas</i>, and <i>Rhodanobacter</i> being significantly enriched. Further, <i>Pseudomonas</i> was identified as a potential key taxon linked to pathogen suppression. Metagenomic analysis showed that, compared to the conducive soil, the microbiome in the disease suppressive soil displayed a significantly greater incidence of genes related to quorum sensing, biofilm formation, and synthesis of antimicrobial compounds potentially active against Foc4. We also recovered a higher frequency of antagonistic <i>Pseudomonas</i> isolates from disease suppressive experimental field sites, and their protective effects against banana <i>Fusarium</i> wilt disease were demonstrated under greenhouse conditions. Despite differences in location and soil conditions, separately located suppressive soils shared common characteristics, including enrichment of Myxococcales, Pseudomonadales, and Xanthomonadales, and enrichment of specific <i>Pseudomonas</i> populations with antagonistic activity against the pathogen. Moreover, changes in functional capacity toward an increase in quorum sensing, biofilm formation, and antimicrobial compound synthesizing involve in disease suppression.</p>","PeriodicalId":520765,"journal":{"name":"Research (Washington, D.C.)","volume":" ","pages":"9818073"},"PeriodicalIF":11.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33491611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}