Nephrology (Carlton, Vic.)最新文献_第8页

MSC-Derived Exosomal MiR-127-3p Alleviates Acute Kidney Ischemia-Reperfusion Injury via Suppressing ATG5/ATG7-Mediated Autophagy. msc来源的外泌体MiR-127-3p通过抑制ATG5/ atg7介导的自噬减轻急性肾缺血再灌注损伤

Nephrology (Carlton, Vic.) Pub Date : 2025-06-01 DOI: 10.1111/nep.70054

Xiaobing Ji, Shuai Nie, Xiangxiang Li, Hao Liu, Xin Du, Li Fan

{"title":"MSC-Derived Exosomal MiR-127-3p Alleviates Acute Kidney Ischemia-Reperfusion Injury via Suppressing ATG5/ATG7-Mediated Autophagy.","authors":"Xiaobing Ji, Shuai Nie, Xiangxiang Li, Hao Liu, Xin Du, Li Fan","doi":"10.1111/nep.70054","DOIUrl":"https://doi.org/10.1111/nep.70054","url":null,"abstract":"Aim: Mesenchymal stem cell-derived exosome (MSC-exo) has garnered increasing attention because of its therapeutic potential for acute kidney injury (AKI). The aim of this study is to investigate the regulatory mechanism of MSC-exo in ischaemic AKI.Methods: We first isolated MSC-exo and identified its properties through transmission electron microscopy observation, nanoparticle tracking analysis, PKH26 staining and Western blot analysis of surface markers. We also determined the therapeutic effect of MSC-exo on AKI through establishing an ischemia-reperfusion (I/R) animal model. The cell model was also constructed by hypoxia-reperfusion (H/R) and used for functional assays. Student's t-test and one-way ANOVA were used to analyse the data.Results: MSC-exo could alleviate the I/R-induced renal injury. In a cell model, MSC-exo could enhance proliferation, reduce apoptosis and block autophagy. Subsequently, miR-127-3p was determined to be transmitted by MSC-exo into injured renal cells to alleviate renal cell injury. Mechanism investigation revealed that miR-127-3p could directly target KIF3B to inactivate the Gli1-hedgehog pathway and thus transcriptionally inactivate ATG5 and ATG7.Conclusion: MiR-127-3p transmitted by MSC-exo alleviates renal cell injury in AKI by suppressing ATG5/ATG7-mediated autophagy. This study explored a new molecular pathway associated with the therapeutic effect of MSC-exo on AKI, which might highlight the MSC-based therapeutic strategy for AKI.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":"30 6","pages":"e70054"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nephrology-Oriented Point of Care Ultrasound Education and Subsequence: Clinical Usefulness for Nephrology Fellows From a Japanese Teaching Hospital. 肾内科护理点超声教育及后续：日本某教学医院肾内科研究员的临床应用。

Nephrology (Carlton, Vic.) Pub Date : 2025-06-01 DOI: 10.1111/nep.70060

Masafumi Sakai, Keisuke Yoshida, Kana Shirai, Fumiya Kitano, Yugo Shibagaki, Masahiko Yazawa

{"title":"Nephrology-Oriented Point of Care Ultrasound Education and Subsequence: Clinical Usefulness for Nephrology Fellows From a Japanese Teaching Hospital.","authors":"Masafumi Sakai, Keisuke Yoshida, Kana Shirai, Fumiya Kitano, Yugo Shibagaki, Masahiko Yazawa","doi":"10.1111/nep.70060","DOIUrl":"10.1111/nep.70060","url":null,"abstract":"Aim: Point-of-care ultrasound (POCUS) is an essential bedside tool in nephrology for evaluation of fluid volume, acute kidney injury (AKI), and vascular access. In Japan, nephrology-oriented POCUS education remains underdeveloped, despite its clinical significance. This study aimed to evaluate the effects of a POCUS educational programme on POCUS implementation in clinical nephrology.Methods: This single-centre observational study was conducted between 2022 and 2024 and comprised 162 patients with AKI. We compared POCUS use, including the checking of the inferior vena cava diameter, urinary obstruction, and venous excess ultrasound (VExUS) grading system, before and after lectures and hands-on training in 2023.Results: There was a significant increase in POCUS implementation for AKI evaluation, including VExUS for assessing congestion. Post-training, the procedure implementation of VExUS for patients with congestion (N = 29) increased from 0% in 2022 to 66.7% in 2023 and 56.3% in 2024. Severe congestion by VExUS might be related to poor outcomes, including higher rates of haemodialysis and mortality. Despite technical difficulties in visualising renal interlobular veins using pulse-wave Doppler in VExUS, the visualisation rate improved over time, increasing from 50% in 2023 to 66.7% in 2024.Conclusions: These results underscore the importance of developing comprehensive nephrology-oriented POCUS training programmes, even at the facility level, coupled with on-the-job training to improve clinical use. However, the procedure implementation of VExUS plateaued in years when the lectures were not conducted. Thus, regularly conducted lectures, the development of supervising physicians' teaching skills, and the implementation of a robust training system would be necessary.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":"30 6","pages":"e70060"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Registry-Based Living Kidney Donor Follow-Up in Australia: An ANZLKD Analysis 2003-2021. 澳大利亚基于登记的活体肾供者随访：2003-2021年ANZLKD分析。

Nephrology (Carlton, Vic.) Pub Date : 2025-06-01 DOI: 10.1111/nep.70065

Neesan George David, Venkat Vangaveti, Monica Suet Ying Ng, Andrew John Mallett

引用次数: 0

RETRACTION: Endostatin Expression in the Murine Model of Ischaemia/Reperfusion-Induced Acute Renal Failure. 收缩：内皮抑素在缺血/再灌注诱导急性肾功能衰竭小鼠模型中的表达。

IF 1.9

Nephrology (Carlton, Vic.) Pub Date : 2025-04-01 DOI: 10.1111/nep.70027

引用次数: 0

Corrigendum to Associations of coenzyme Q10 with endothelial function in hemodialysis patients. 血液透析患者中辅酶Q10与内皮功能相关性的勘误表。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 DOI: 10.1111/nep.14128

引用次数: 0

Infectious complications in adult patients with idiopathic minimal change nephrotic syndrome undergoing immunosuppressive therapy. 接受免疫抑制治疗的特发性微小变化肾病综合征成人患者的感染并发症。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 Epub Date: 2022-10-19 DOI: 10.1111/nep.14119

Chih-Yang Hsu, Chung Chang, Hsin-Yu Chen, Shih-Hsiang Ou, Kang-Ju Chou, Hua-Chang Fang, Chien-Liang Chen, Chien-Wei Huang, Tzung-Yo Ho, Po-Tsang Lee

{"title":"Infectious complications in adult patients with idiopathic minimal change nephrotic syndrome undergoing immunosuppressive therapy.","authors":"Chih-Yang Hsu, Chung Chang, Hsin-Yu Chen, Shih-Hsiang Ou, Kang-Ju Chou, Hua-Chang Fang, Chien-Liang Chen, Chien-Wei Huang, Tzung-Yo Ho, Po-Tsang Lee","doi":"10.1111/nep.14119","DOIUrl":"https://doi.org/10.1111/nep.14119","url":null,"abstract":"Background: Patients with idiopathic minimal change nephrotic syndrome (MCNS) undergoing immunosuppressive therapy are susceptible to infectious complications. Study specifically focusing on adult population's infectious complications is lacking.Methods: We retrospectively collected 101 adult patients with biopsy-proven idiopathic MCNS and analysed for the infectious complications. Published literatures were also reviewed aiming to evaluate the feasibility of prophylactic antibiotic treatment.Results: Infectious complications developed in 17 of 101 (16.8%) patients, with pneumonia (n = 4), cellulitis/fasciitis (n = 4) and urinary tract infection (UTI) (n = 4) being the dominant diseases, and Gram-negative bacilli the main cause. AKI stage ≥2 (Hazard ratio = 6.1; 95% CI: 1.2-31.9, p = 0.031) and non-remission by treatment (Hazard ratio = 4.4; 95% CI: 1.2-15.6, p = .023) were the two independent risk factors relevant to developing infectious complications. Review of 16 published literatures and our data showed that even no prophylactic antibiotic therapy, only one case of Pneumocystis jirovecii pneumonia developed among the 1787 accumulative cases of MCNS. In contrast, 16 (44%) of acute flare cases were reported among the 36 patients with positive hepatitis B surface antigen that did not receive antiviral prophylactic therapy.Conclusions: Advanced acute kidney injury and non-remission by treatment are the risk factors toward developing infectious complications in adult MCNS undergoing immunosuppressive therapy. It appears unnecessary to use prophylactic antibiotic for Pneumocystis jirovecii pneumonia or other bacterial infections, while screening and prophylactic therapy for hepatitis B and latent tuberculosis are critical for patients in prevalent area.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"953-961"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33494093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircHOMER1 aggravates oxidative stress, inflammation and extracellular matrix deposition in high glucose-induced human mesangial cells. CircHOMER1加重高糖诱导的人系膜细胞的氧化应激、炎症和细胞外基质沉积。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 Epub Date: 2022-10-18 DOI: 10.1111/nep.14115

Shi Shu, Zhongju Xu, Haiying Lu, Zhijie Li, Yue Zhang

{"title":"CircHOMER1 aggravates oxidative stress, inflammation and extracellular matrix deposition in high glucose-induced human mesangial cells.","authors":"Shi Shu, Zhongju Xu, Haiying Lu, Zhijie Li, Yue Zhang","doi":"10.1111/nep.14115","DOIUrl":"https://doi.org/10.1111/nep.14115","url":null,"abstract":"Background: Circular RNAs (circRNAs) play an important regulatory role in human diseases, including diabetic nephropathy (DN). The purpose of this study was to investigate the role and mechanism of circHOMER1 action in DN.Methods: Human mesangial cells (HMCs) were tested with high glucose (HG) to mimic DN cell models. Quantitative real-time PCR was performed to determine circHOMER1, microRNA (miR)-137 and SRY-box transcription factor 6 (SOX6) expression. SOD activity and MDA level were detected to evaluate cell oxidative stress. ELISA assay was used to analyse the levels of inflammation factors. The protein levels of extracellular matrix (ECM) deposition-related markers and SOX6 were assessed by western blot analysis. The interaction between miR-137 and circHOMER1 or SOX6 was analysed by dual-luciferase reporter assay and RNA pull-down assay.Results: CircHOMER1 was highly expressed in HG-induced HMCs and DN patients. Downregulation of circHOMER1 suppressed oxidative stress, inflammation and ECM deposition in HMCs induced by HG. In terms of mechanism, circHOMER1 could sponge miR-137 to regulate SOX6. Function assays showed that miR-137 inhibitor or SOX6 overexpression revoked the negative regulation of circHOMER1 knockdown on HG-induced HMCs injury. In addition, miR-137 expression was negatively correlated with circHOMER1 and SOX6 expression in DN patients.Conclusion: CircHOMER1 promoted HG-induced HMCs oxidative stress, inflammation and ECM accumulation via the miR-137/SOX6 axis, suggesting that circHOMER1 might be a target for DN treatment.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"983-993"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40387321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Seasonality in hip fracture among haemodialysis patients and kidney transplant recipients in South Korea. 韩国血液透析患者和肾移植受者髋部骨折的季节性。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 Epub Date: 2022-09-30 DOI: 10.1111/nep.14110

Hye Eun Yoon, Yeon Hee Lee, Joo Eun Lee, Jungkuk Lee, Hoseob Kim, Byung Ha Chung, Seok Joon Shin

{"title":"Seasonality in hip fracture among haemodialysis patients and kidney transplant recipients in South Korea.","authors":"Hye Eun Yoon, Yeon Hee Lee, Joo Eun Lee, Jungkuk Lee, Hoseob Kim, Byung Ha Chung, Seok Joon Shin","doi":"10.1111/nep.14110","DOIUrl":"https://doi.org/10.1111/nep.14110","url":null,"abstract":"Aim: The seasonality of hip fracture in haemodialysis (HD) patients and kidney transplant recipients (KTRs) have not been reported. We assessed seasonal variations in hip fractures among patients with end-stage kidney disease who undergo maintenance HD and KTRs.Methods: Using the Korean National Health Insurance System database from January 2012 to December 2017, monthly counts of hip fracture were calculated among HD patients (n = 77 420) and KTRs (n = 8921). The 6-year normalized monthly fraction and seasonal fractions of hip fractures were calculated. A cosinor analysis was performed to determine the seasonality of the monthly incidence of hip fractures.Results: The 6-year average monthly fraction of hip fractures was lowest in June and highest in October in HD patients, and lowest in February and highest in November in KTRs. The 6-year average seasonal fraction among HD patients was lowest in summer and highest in winter, and lowest in summer and highest in autumn among KTRs, but there was no significant difference. The incidence ratio of hip fractures was lowest in June and highest in January in HD patients, and lowest in August and highest in November in KTRs. On cosinor analysis, HD patients showed significant seasonality in hip fracture incidence, with a trough in summer and a peak in winter (p = .031), whereas KTRs did not exhibit a significant trend (p = .44).Conclusion: Hip fractures occurred more frequently in winter and less frequently in summer in patients undergoing HD, whereas KTRs did not show a seasonal trend.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"925-933"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33470832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and risk factors for cognitive dysfunction in peritoneal dialysis patients. 腹膜透析患者认知功能障碍的发生频率及危险因素。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 Epub Date: 2022-10-13 DOI: 10.1111/nep.14117

Isuru Gamage, Arup Dhar, Peter Tregaskis, Scott Wilson

{"title":"Frequency and risk factors for cognitive dysfunction in peritoneal dialysis patients.","authors":"Isuru Gamage, Arup Dhar, Peter Tregaskis, Scott Wilson","doi":"10.1111/nep.14117","DOIUrl":"https://doi.org/10.1111/nep.14117","url":null,"abstract":"Objective: There is limited data on cognition in patients undergoing peritoneal dialysis (PD). We assessed prevalence and associated risk factors of neurocognitive impairment (NCI) in PD patients.Design and methods: A cross-sectional cohort study of 149 PD patients at a single centre between 2016 and 2020 who underwent neurocognitive screening at defined intervals by Addenbrooke's Cognitive Examination - Revised (ACE-R) with incorporated Mini-Mental State Examination (MMSE). Paired-sample t-test was used to compare cognitive performance to the general population and compare cohorts for dichotomous risk factors. Residual renal function (RRF) and clearance kinetics were evaluated using local regression models. Sub-analysis was performed in patients with cerebrovascular disease (CVD).Results: Patients on PD performed poorly in ACE-R screening compared to population norms, with discrepancy in all cognitive domains. In patients without CVD, attention and language domains were comparable to norms. The MMSE detected cognitive impairment in 2% of studied patients, significantly fewer than when the ACE-R was applied (32%). Age, gender, diabetic status and depression were associated with lower neurocognitive screening performance (p < .05). Dialysis vintage beyond 12 months conferred poorer cognitive performance. RRF correlated with cognitive performance.Conclusion: Patients on PD have higher prevalence of NCI than the general population, primarily with impairments in memory, fluency and visuospatial reasoning. CVD confers poorer performance in attention and language domains. The MMSE is ineffective in detecting subtle NCI in this population compared with ACE-R. Risk factors for NCI include age, gender, diabetic status, depression and vintage beyond 12 months. Protective factors include RRF.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"945-952"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40393906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of kidney transplant outcomes in HLA compatible and incompatible transplantation: A national cohort study. HLA相容和HLA不相容肾移植结果的比较:一项国家队列研究。

IF 2.5

Nephrology (Carlton, Vic.) Pub Date : 2022-12-01 Epub Date: 2022-09-21 DOI: 10.1111/nep.14102

Trijntje J W Rennie, Richard K Battle, Angela A Abel, Sylvia McConnell, Robert McLaren, Paul J Phelan, Colin Geddes, Neal Padmanabhan, Marc J Clancy, Ann-Margaret Little, David M Turner

{"title":"Comparison of kidney transplant outcomes in HLA compatible and incompatible transplantation: A national cohort study.","authors":"Trijntje J W Rennie, Richard K Battle, Angela A Abel, Sylvia McConnell, Robert McLaren, Paul J Phelan, Colin Geddes, Neal Padmanabhan, Marc J Clancy, Ann-Margaret Little, David M Turner","doi":"10.1111/nep.14102","DOIUrl":"https://doi.org/10.1111/nep.14102","url":null,"abstract":"Background: Reports of HLA incompatible (HLAi) kidney transplant outcomes are inconclusive, especially in the context of lower level Donor Specific Antibodies (DSA).Methods: Multi-centre national cohort study of HLAi kidney transplant recipients matched in 1:2 ratio with HLA compatible (HLAc) kidney transplant recipients. HLAi defined as DSA identified by Luminex. Antibody mediated rejection (AMR) and transplant-survival were analysed using Kaplan-Meier plots. Propensity score (PS) matching was used to compare recipient and transplant survival between groups.Results: We included 61 HLAi and 122 HLAc recipients; mean age 46 years; 60% female. MFIT0 : 3327 (IQR 1352-6458), 23 (38%) were Flow cytometry crossmatch positive (FC-XMPOS ). DSAPOS /FC-XMPOS transplantation carried an increased risk of AMR at 1 year (52%) compared to DSAPOS /FC-XMNEG (27%) and HLAc (0%). Unadjusted death censored graft loss at 3 years was 13% (HLAi) and 8% (HLAc). Three-year patient survival was 95% in HLAc, 84% in DSAPOS /FC-XMNEG and 69% in DSAPOS /FC-XMPOS recipients; 58% of HLAi deaths were infection-related. HLA incompatibility was associated with a decreased 3-year survival in our PS-matched cohort.Conclusion: In kidney transplantation, DSA and positive FC-XM carries an increased risk of AMR. Despite inferior transplant and survival outcomes compared to HLAc transplantation, it remains a realistic option for highly sensitized patients facing prolonged waiting times and reduced survival on dialysis.","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"962-972"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40644722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2