Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism最新文献

Characterizing pre- and post-operative cerebral blood flow and transit time in pediatric moyamoya patients using multi-delay ASL and DSC MRI. 应用多延迟ASL和DSC MRI表征小儿烟雾病患者术前和术后脑血流量和传递时间。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-07-15 DOI: 10.1177/0271678X251358979

Moss Y Zhao, Sasha Alexander, Chris Antonio Lopez, Helena Zhang, Gabriella Morton, Rui Duarte Armindo, Kristen W Yeom, Elizabeth Tong, Bruno P Soares, Sarah Lee, Michael Moseley, Gary K Steinberg

{"title":"Characterizing pre- and post-operative cerebral blood flow and transit time in pediatric moyamoya patients using multi-delay ASL and DSC MRI.","authors":"Moss Y Zhao, Sasha Alexander, Chris Antonio Lopez, Helena Zhang, Gabriella Morton, Rui Duarte Armindo, Kristen W Yeom, Elizabeth Tong, Bruno P Soares, Sarah Lee, Michael Moseley, Gary K Steinberg","doi":"10.1177/0271678X251358979","DOIUrl":"https://doi.org/10.1177/0271678X251358979","url":null,"abstract":"Cerebral blood flow (CBF) and transit time are essential biomarkers for assessing brain health. While dynamic susceptibility contrast (DSC) MRI has been widely applied to measure these metrics, it is limited in the pediatric population due to the need for contrast agents. Arterial spin labeling is a non-invasive and quantitative MR modality, and multi-delay ASL can measure CBF and transit time simultaneously. Although multi-delay ASL has been used in adult neuroimaging studies, its application in children requires investigation. Moyamoya disease, a progressive steno-occlusive cerebrovascular disorder, often manifests in childhood. In this work, we present a cohort study that examines multi-delay ASL and DSC MRI to characterize vascular hemodynamics in 22 pediatric patients. We evaluate CBF and transit time in different brain regions before and after revascularization surgeries. Results show that revascularization significantly increased CBF by 24% and 7.6%, respectively, as measured by ASL and DSC; it also significantly decreased transit time by 12% and 15%, indicating improved hemodynamics and metabolism. ASL and DSC results also showed significantly positive correlations in all brain regions. Thus, revascularization improved hemodynamics in pediatric moyamoya patients and shows that multi-delay ASL can effectively characterize CBF and transit time in the pediatric population.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251358979"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between cerebrovascular risk factors and oxygen metabolic stress in a cognitively impaired population: Dynamic susceptibility contrast-derived oxygen parametric analysis. 认知障碍人群中脑血管危险因素与氧代谢应激的关系：动态易感性对比衍生的氧参数分析

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-07-08 DOI: 10.1177/0271678X251355742

Dongjun Lee, Yangsean Choi, Eunseon Jeong, Ji Eun Park, Ho-Sung Kim

{"title":"Relationship between cerebrovascular risk factors and oxygen metabolic stress in a cognitively impaired population: Dynamic susceptibility contrast-derived oxygen parametric analysis.","authors":"Dongjun Lee, Yangsean Choi, Eunseon Jeong, Ji Eun Park, Ho-Sung Kim","doi":"10.1177/0271678X251355742","DOIUrl":"10.1177/0271678X251355742","url":null,"abstract":"Cerebrovascular risk factors contribute to cognitive decline via mechanisms such as small vessel disease and chronic ischemia. Advanced MRI parameters, including maximum oxygen extraction fraction (OEFmax) and cerebral metabolic rate of oxygen (CMRO2), provide insights into cerebral oxygenation and cognition. This retrospective study evaluated the relationships between oxygen metabolic stress, cerebrovascular risk factors, and cognitive function in 226 adults undergoing dynamic susceptibility contrast (DSC)-MRI. White matter hyperintensity (WMH) volumes, OEFmax, and CMRO2 were quantified and analyzed against cerebrovascular risk factors (e.g., age, smoking, hypertension), MMSE scores, clinical diagnoses, and history of cerebrovascular disease. A history of smoking was linked to a higher OEFmax (P = 0.015). An older age correlated with a larger WMH volume (P < 0.001), higher OEFmax (P < 0.001), and lower CMRO2 (P = 0.001). Patients with history of infarcts exhibited larger WMH volumes (P < 0.001) and lower CMRO2 (P < 0.001). Multivariable regression showed WMH volume (P = 0.03) and OEFmax (P = 0.018) were negatively associated with MMSE scores, while CMRO2 had no significant association (P = 0.52). Patients with vascular dementia exhibited lower CMRO2 than those with Alzheimer's or mild cognitive impairment (P = 0.001). These findings suggest that DSC-derived OEFmax and CMRO2 could provide insights into the relationship between cerebrovascular risk factors and cognitive decline, emphasizing their role as key indicators of brain oxygenation.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251355742"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2-deoxy-D-glucose chemical exchange-sensitive spin-lock MRI of cerebral glucose metabolism after transient focal stroke in the rat. 短暂性局灶性脑卒中后2-脱氧-d -葡萄糖化学交换敏感自旋锁MRI研究。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-07-08 DOI: 10.1177/0271678X251355049

Philipp Boehm-Sturm, Patrick Schuenke, Marco Foddis, Susanne Mueller, Stefan P Koch, Daniel J Beard, Paul Holloway, Amin Mottahedin, Leif Schröder, Alastair M Buchan, Philipp Mergenthaler

{"title":"2-deoxy-D-glucose chemical exchange-sensitive spin-lock MRI of cerebral glucose metabolism after transient focal stroke in the rat.","authors":"Philipp Boehm-Sturm, Patrick Schuenke, Marco Foddis, Susanne Mueller, Stefan P Koch, Daniel J Beard, Paul Holloway, Amin Mottahedin, Leif Schröder, Alastair M Buchan, Philipp Mergenthaler","doi":"10.1177/0271678X251355049","DOIUrl":"10.1177/0271678X251355049","url":null,"abstract":"Rapid breakdown of cerebral glucose metabolism is a hallmark in stroke pathology. Metabolic activity delineates the penumbra from the infarct core, representing tissue that is potentially salvageable by therapeutic interventions. Tools to image dynamics of glucose and its spatial distribution could provide biomarkers of disease severity and of the success of therapeutic interventions. Here, we developed a new protocol to measure glucose transport and metabolism in a rat model of stroke using chemical exchange-sensitive spin-lock (CESL) MRI of the glucose analogue 2-deoxy-D-glucose (2DG). We further implemented a protocol that combines 2DG-CESL-MRI with perfusion and diffusion MRI to relate this new signal to established definitions of hypoperfused tissue, cytotoxic edema and the penumbra. We found that 2DG-CESL-MRI provides a biomarker of disturbed glucose transport and metabolism after stroke with high effect size. This is the first study to investigate CESL MRI of 2DG in the context of transport and metabolism imaging in rodent stroke.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251355049"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurovascular coupling assessment by transcranial doppler in acute stroke could be informative of long-term cognitive status. 经颅多普勒评价急性脑卒中患者的神经血管耦合可以提供长期认知状态的信息。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-07-04 DOI: 10.1177/0271678X251352695

Juliana Ferreira, Frederica Alves, Tiago Pedro, Luísa Fonseca, Guilherme Gama, Goreti Moreira, Elsa Azevedo, Pedro Castro

{"title":"Neurovascular coupling assessment by transcranial doppler in acute stroke could be informative of long-term cognitive status.","authors":"Juliana Ferreira, Frederica Alves, Tiago Pedro, Luísa Fonseca, Guilherme Gama, Goreti Moreira, Elsa Azevedo, Pedro Castro","doi":"10.1177/0271678X251352695","DOIUrl":"10.1177/0271678X251352695","url":null,"abstract":"To assess whether Neurovascular Coupling (NVC) status in acute ischemic stroke predicts long-term cognitive impairment, this prospective study included consecutive ischemic stroke patients, without prior cognitive deficits, recruited between September 2021 and August 2022. Transcranial Doppler (TCD) monitoring was performed within 72 hours post-stroke to measure NVC, determined by peak relative increase in cerebral blood velocity (CBv) in the posterior cerebral artery (PCA) during a visual stimulus. Age-, gender-, and vascular risk factor-matched controls were included. The primary outcome was cognitive status at 12 months, evaluated using a 7-point cognitive scale. A total of 144 acute stroke patients and 40 controls were included. NVC exhibited a U-shaped association with long-term cognitive outcomes. Patients in both the lowest (CI95% 2.29 [1.23-4.28], p < 0.01) and highest (CI95% 0.53 [0.29-0.98], p = 0.04) quintiles of NVC magnitude had significantly worse cognitive scores at 12 months compared to those in the reference quintile. NVC assessment via TCD in the acute phase of ischemic stroke may serve as a predictor of long-term cognitive impairment, suggesting that both diminished and exaggerated NVC responses are linked to poorer cognitive outcomes.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251352695"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperglycemia selectively increases cerebral non-oxidative glucose consumption without affecting blood flow. 高血糖症选择性地增加大脑非氧化葡萄糖消耗而不影响血流。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-07-01 DOI: 10.1177/0271678X251329714

Tyler Blazey, John J Lee, Abraham Z Snyder, Manu S Goyal, Tamara Hershey, Ana Maria Arbeláez, Marcus E Raichle

{"title":"Hyperglycemia selectively increases cerebral non-oxidative glucose consumption without affecting blood flow.","authors":"Tyler Blazey, John J Lee, Abraham Z Snyder, Manu S Goyal, Tamara Hershey, Ana Maria Arbeláez, Marcus E Raichle","doi":"10.1177/0271678X251329714","DOIUrl":"10.1177/0271678X251329714","url":null,"abstract":"Multiple studies have shown that hyperglycemia increases the cerebral metabolic rate of glucose (CMRglc) in subcortical white matter. This observation remains unexplained. Using positron emission tomography (PET) and pancreatic glucose clamps with basal insulin replacement in twenty-nine healthy young adults (34.5 years, SD = 10.1) we found that acute hyperglycemia increases non-oxidative CMRglc (i.e., aerobic glycolysis (AG)) in subcortical white mater as well as in medial temporal lobe structures, cerebellum and brainstem, all areas with low CMRglc during euglycemia. Surprisingly, hyperglycemia did not change regional cerebral blood flow (CBF), the cerebral metabolic rate of oxygen (CMRO2), or the blood-oxygen-level-dependent (BOLD) response. Correlation with existing regional gene expression data showed that brain regions where CMRglc increased have greater expression of hexokinase 2 (HK2). Simulations of glucose transport revealed that, unlike hexokinase 1, HK2 is not saturated at euglycemia, and thus can accommodate increased AG during hyperglycemia.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251329714"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the effects of aging on the Koizumi's and Zea Longa's methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study. 评估衰老对小泉和Zea Longa方法的影响及其作为评估缺血性中风后神经变性的小鼠模型的适用性：一项比较研究。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-06-26 DOI: 10.1177/0271678X251352691

Chiyeon Lim, Hongrae Kim, Sehyun Lim, Hyo-Eun Kim, So-Jung Moon, Hyung-Hwan Kim, Suin Cho

{"title":"Assessing the effects of aging on the Koizumi's and Zea Longa's methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study.","authors":"Chiyeon Lim, Hongrae Kim, Sehyun Lim, Hyo-Eun Kim, So-Jung Moon, Hyung-Hwan Kim, Suin Cho","doi":"10.1177/0271678X251352691","DOIUrl":"10.1177/0271678X251352691","url":null,"abstract":"Koizumi's and Zea Longa's middle cerebral artery occlusion (MCAO) models (KMCAO and LMCAO) are commonly used methods in rodent ischemic stroke research. A key distinction between them lies in whether the blood flow through the common carotid artery (CCA) is maintained. Limited evidence exists regarding the systematic comparison of their pathophysiological characteristics, particularly considering the age of experimental mice. In this study, cerebral infarction outcomes were analyzed in mice aged 3, 6, 9, and 12 months. Mice aged 3-6 months exhibited minor cerebral infarction with KMCAO but substantial infarction with LMCAO, whereas differences diminished in 9-12-month-old mice. Comparable infarct severity in 3-month-old mice was achieved by inducing ischemia for 1.5 hours using KMCAO and for 1 hour using LMCAO, suggesting potential mechanistic similarities, subject to further research. LMCAO led to reperfusion injury due to preserved CCA perfusion, whereas KMCAO provided controlled ischemic insult via continuous CCA ligation. Both models confirmed that increased brain damage correlated with decreased neuronal nuclei expression and increased glial fibrillary acidic protein expression. These findings highlight the importance of selecting the MCAO model based on experimental objectives, age, and ischemia duration for the accurate modeling of ischemic and degenerative brain injury.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251352691"},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of hyperglycemia on brain physiology in a healthy and injured state: An experimental pig study with state-of-the-art multimodal neuromonitoring. 健康和受伤状态下高血糖对脑生理的影响：一项采用最先进的多模式神经监测的实验猪研究。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-06-26 DOI: 10.1177/0271678X251337633

Teodor Svedung Wettervik, Anders Hånell, Kerstin M Ahlgren, Henrik Engquist, Anders Lewén

{"title":"The effects of hyperglycemia on brain physiology in a healthy and injured state: An experimental pig study with state-of-the-art multimodal neuromonitoring.","authors":"Teodor Svedung Wettervik, Anders Hånell, Kerstin M Ahlgren, Henrik Engquist, Anders Lewén","doi":"10.1177/0271678X251337633","DOIUrl":"10.1177/0271678X251337633","url":null,"abstract":"Although hyperglycemia is associated with worse outcome following acute brain injury, the pathomechanisms remain elusive. In this experimental pig study, we explored the effects of hyperglycemia on brain physiology. Six pigs were anesthetized and received multimodal neuromonitoring of intracranial pressure (ICP), cerebral perfusion pressure (CPP), cerebral autoregulatory metrics (PRx, CBFx, ORx, and with transfer function analysis), cerebral blood flow (CBF), partial brain tissue oxygenation (pbtO2), and cerebral microdialysis (MD). The effect of hyperglycemia was studied in the healthy brain after injection of intravenous glucose injections, which increased MD-glucose, while ICP, CPP, PRx, CBFx, ORx pbtO2, and cerebral energy metabolism remained unchanged. After normalization of arterial glucose, an intracranial balloon was inflated to increase ICP, followed by an intravenous glucose injection to study the effect of hyperglycemia in the injured brain. The latter induced a significant CBF elevation, but no changes in PRx, pbtO2, or cerebral energy metabolism (but a trend towards higher glucose). Hyperglycemia led to favorable short-term effects on cerebral physiology and the immediate increase in arterial glucose that usually follows acute brain injury may be physiologically neuroprotective and the detrimental role of hyperglycemia is more likely related to cellular and molecular pathophysiological mechanisms or merely a confounder.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251337633"},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain water dynamics across sleep stages measured by near-infrared spectroscopy: Implications for glymphatic function. 近红外光谱测量睡眠阶段的脑水动力学：对淋巴功能的影响。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-06-25 DOI: 10.1177/0271678X251353142

Jee-Eun Yoon, Minsu Ji, Inha Hwang, Woo-Jin Lee, Seongkwon Yu, Jaemyoung Kim, Chanhyung Lee, Haeil Lee, Bumjun Koh, Hyeonmin Bae, Chang-Ho Yun

{"title":"Brain water dynamics across sleep stages measured by near-infrared spectroscopy: Implications for glymphatic function.","authors":"Jee-Eun Yoon, Minsu Ji, Inha Hwang, Woo-Jin Lee, Seongkwon Yu, Jaemyoung Kim, Chanhyung Lee, Haeil Lee, Bumjun Koh, Hyeonmin Bae, Chang-Ho Yun","doi":"10.1177/0271678X251353142","DOIUrl":"10.1177/0271678X251353142","url":null,"abstract":"This study investigates brain water dynamics across the sleep-wake cycle using near-infrared spectroscopy (NIRS) and linear mixed-effects modeling, motivated by prior observations that glymphatic activity increases during non-rapid eye movement (NREM) and decreases during REM sleep. Forty-one healthy volunteers underwent polysomnography with concurrent cerebral NIRS, with measurements taken 30 minutes before sleep, throughout the night, and for 60 minutes after waking. Brain water content (arbitrary unit, A.U.) was block-averaged for 5-minute epochs and analyzed across WAKE→NREM, NREM→WAKE, NREM→REM, and REM→NREM transitions. Water content significantly increased during WAKE→NREM (0.57 A.U., d = 0.77, p < 0.001) and decreased during NREM→WAKE (-0.93 A.U., d = -1.25, p < 0.001). Decreases during NREM→REM (-0.40 A.U., d = -0.53, p < 0.05) were followed by increases during REM→NREM (0.62 A.U., d = 1.10, p < 0.001). Brain water accumulation was significantly greater during the first compared to the last NREM cycle (0.70 A.U., d = 0.86, p < 0.01). These findings reveal robust, state-dependent fluctuations in brain water content that parallel established glymphatic physiology. Water-sensitive NIRS may offer a promising non-invasive approach to monitoring sleep-related brain fluid dynamics in humans, though further multimodal studies are needed to determine its specificity for glymphatic activity.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251353142"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capillaries susceptible to frequent stall dynamics revealed by comparing OCT and bessel-2PM measurements. 通过比较OCT和贝塞尔- 2pm测量，发现毛细血管易受频繁失速动力学的影响。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-06-25 DOI: 10.1177/0271678X251353141

Rockwell P Tang, Shannon Kelley, Gülce Küreli, Emily A Long, Piergiulio R Bressan, Shashwat Shah, Şefik E Erdener, John Jiang, John T Giblin, Sreekanth Kura, Matthew G Simkulet, Bradley C Rauscher, Chris B Schaffer, Nozomi Nishimura, Martin Thunemann, Anna Devor, David A Boas

{"title":"Capillaries susceptible to frequent stall dynamics revealed by comparing OCT and bessel-2PM measurements.","authors":"Rockwell P Tang, Shannon Kelley, Gülce Küreli, Emily A Long, Piergiulio R Bressan, Shashwat Shah, Şefik E Erdener, John Jiang, John T Giblin, Sreekanth Kura, Matthew G Simkulet, Bradley C Rauscher, Chris B Schaffer, Nozomi Nishimura, Martin Thunemann, Anna Devor, David A Boas","doi":"10.1177/0271678X251353141","DOIUrl":"10.1177/0271678X251353141","url":null,"abstract":"Transient stoppages of red blood cell (RBC) flow through capillaries-termed capillary stalls-occur persistently in neurological disorders such as Alzheimer's disease and ischemic stroke and can interrupt oxygen delivery and exacerbate neurological damage. Effective imaging tools and analyses are necessary to understand the nature, role, and prevention of stalls. In this study, we dissect differences in stalls measured by two-photon Bessel beam microscopy (Bessel-2PM) and optical coherence tomography (OCT) to gain insight into the temporal dynamics of stalls. Twenty-minute series of volumetric angiograms were obtained separately with Bessel-2PM and OCT on the same day in awake, head-fixed mice. The temporal dynamics of stalling in both methods revealed a minority population of susceptible capillaries that exhibited frequent stalls and a large majority of capillaries with infrequent stalls. Differences between OCT and Bessel-2PM in the repeatability and dynamics of stalls are explained by differences in their sensitivity to short or infrequent stalls based on scanning speed and detection off-time. Finally, stroke caused a shift toward the frequently stalling capillary subpopulation, lasting 1 week post-stroke. Dynamic stall analysis therefore enables examination of physiological and methodological contributions to the stalls measured in disease models and across studies.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251353141"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EC₅₀ images reveal reproducible spatial variation in drug affinity across single- and repeat-dose occupancy studies. EC50图像显示单次和重复剂量占用研究中药物亲和力的可重复空间变化。

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Pub Date : 2025-06-25 DOI: 10.1177/0271678X251353140

Alaaddin Ibrahimy, Su Jin Kim, Mark E Schmidt, Mark Slifstein, Erik Mannaert, Randolph D Andrews, Dawn C Matthews, Cristian C Constantinescu, Graham E Searle, Roger N Gunn, Eugenii A Rabiner, Evan D Morris

{"title":"EC50 images reveal reproducible spatial variation in drug affinity across single- and repeat-dose occupancy studies.","authors":"Alaaddin Ibrahimy, Su Jin Kim, Mark E Schmidt, Mark Slifstein, Erik Mannaert, Randolph D Andrews, Dawn C Matthews, Cristian C Constantinescu, Graham E Searle, Roger N Gunn, Eugenii A Rabiner, Evan D Morris","doi":"10.1177/0271678X251353140","DOIUrl":"10.1177/0271678X251353140","url":null,"abstract":"We investigated spatial variation in the apparent affinity of the D2 antagonist drug, JNJ-37822681, for dopamine receptors across single-dose (SD) and repeat-dose (RD) PET occupancy studies. Traditional whole-brain EC50 estimates overlook potential spatial variation in drug affinity. We reanalyzed PET occupancy data from two published studies in healthy male volunteers (SD: administering 1 dose between 2-20 mg; RD: administering 13 doses of 10 mg over 7 days). Voxel-level occupancy images were generated from binding potential maps using Lassen Plot Filter (LPF), clustered via SLIC-Occ, and fitted to one- and two-parameter Emax models to generate EC50 images, revealing regional variation in apparent affinity within the striatum. The two-parameter model incorporated receptor upregulation in a joint analysis of SD and RD data. EC50 images demonstrated reproducible spatial variation in drug affinity across striatal subregions; caudate and ventral striatum showed lower EC50 values than putamen. Additionally, left-right asymmetries in EC50 were detected, once the effects of upregulation were addressed. Our findings validate EC50 images for capturing spatial variation in drug affinity and highlights the importance of accurate modeling in chronic dosing studies. LPF and SLIC-Occ provide a robust framework for analyzing occupancy data, aiding dose optimization for drug trials.","PeriodicalId":520660,"journal":{"name":"Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism","volume":" ","pages":"271678X251353140"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0