Ideggyogyaszati szemle最新文献_第3页

Histochemistry of the extracellular matrix in the snail central nervous system. 蜗牛中枢神经系统细胞外基质的组织化学。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Zoltán Serfozo, Károly Elekes

{"title":"Histochemistry of the extracellular matrix in the snail central nervous system.","authors":"Zoltán Serfozo, Károly Elekes","doi":"","DOIUrl":"","url":null,"abstract":"Even tough the central nervous system (CNS) of gastropods has long been used as a model for studying different neuronal networks underlying behaviors, there is only little information on the molecular components of the extracellular matrix (ECM) of the nervous tissue. Therefore, the aim of the present study was to identify some of the ECM molecules by acid-base histochemistry. Staining with alcian blue at strong acidic pH, and with acridine orange at different pH and salt concentrations was carried out on cryostat sections taken from CNS preparations of adult specimens of the terrestrial snail, Helix pomatia, and the aquatic species, Lymnaea stagnolis, in order to visualize mild (carboxyl) and strong (sulphate) acidic groups, which are characteristic for different glucosaminoglycans. According to our findings, sulphated proteoglycans were abundant in the periganglionic sheath of both species, and they also occurred in the neuropil of Helix, whereas they were absent in Lymnaea. The interperikaryonal space contained mainly carboxyl residues, which might refer to the presence of hyaluronic acid. It is concluded that the ECM of the snail CNS, similarly to that in vertebrates, is partly composed of polymer macromolecules of different chemical properties. It is suggested that adaptation to environmental conditions and/or altered neuronal plasticity are responsible for the differences found in chemical characters of the ECM molecules between the two snail species.","PeriodicalId":520642,"journal":{"name":"Ideggyogyaszati szemle","volume":" ","pages":"173-6"},"PeriodicalIF":0.8,"publicationDate":"2007-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26680755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postnatal expression pattern of doublecortin (DCX) in some areas of the developing brain of mouse. 双皮质素(DCX)在小鼠脑发育部分区域的产后表达模式。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

József Takács, Roberta Zaninetti, Julianna Víg, Csaba Vastagh, József Hámori

引用次数: 0

Central atrial natriuretic peptide in dehydration. 中央房利钠肽脱水。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Udo Bahner, Helmut Geiger, Miklós Palkovits, Zsolt Lenkei, Friedrich C Luft, August Heidland

引用次数: 0

Optimal Alignment novel software procedure for 3D reconstruction of electronmicroscopic serial sections. 电子显微镜连续切片三维重建的优化对齐新软件程序。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

László Simon, Sándor Garab, Annamária Noszek, Elizabeth Römmer, László Záborszky

{"title":"Optimal Alignment novel software procedure for 3D reconstruction of electronmicroscopic serial sections.","authors":"László Simon, Sándor Garab, Annamária Noszek, Elizabeth Römmer, László Záborszky","doi":"","DOIUrl":"","url":null,"abstract":"3D reconstruction from electronmicroscopic (EM) serial sections substantially differs from modeling body parts by linking convoluted planes delivered by CT and NMR. Namely, variations both in relative X-Y position and rotation of the target elements between the adjacent images and also additional problems caused by deformed, deteriorated or missing sections can only be overruled by an aligning paradigm, which exploits all the pixel-level information, and results in an optimal fitting with selected precision. This paper presents a complex computer program called Optimal Alignment, which performs the precise elaboration of X-Y shift and relative rotation of two consecutive images. The required searching process will be customized by setting four independent parameters which relate the span and density of the pixel-scanning basic process. Optimalization of fitting accuracy versus running time can be achieved by a rather short training period. The potential precision of Optimal Alignment based on complex algorithms is far superior to manual aligning of EM photographs with the eye-wrist-mouse facility. The resulted database of alignment orientation parameters can serve as an advanced source for the 3D reconstructing programs. Optimal Alignment software tool (supported by Hungarian Space Office grant TP 138) will be demonstrated on a basal forebrain NPY+ axonal reconstruction, performed in L. Záborszky's laboratory (supported by NIH grant NSO23945).","PeriodicalId":520642,"journal":{"name":"Ideggyogyaszati szemle","volume":" ","pages":"154-8"},"PeriodicalIF":0.8,"publicationDate":"2007-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26680750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activated somatostatin type 2 receptors traffic in vivo from dendrites to the trans-golgi network. 激活的生长抑素2型受体在体内从树突到跨高尔基网络的运输。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Zsolt Csaba, Pascal Dournaud

{"title":"Activated somatostatin type 2 receptors traffic in vivo from dendrites to the trans-golgi network.","authors":"Zsolt Csaba, Pascal Dournaud","doi":"","DOIUrl":"","url":null,"abstract":"Background and purpose: Understanding the trafficking of G-protein-coupled receptors is of particular importance. In the central nervous system, although some G-protein-coupled receptors were reported to internalize in vivo, little is known about their trafficking downstream of the endocytic event.Methods: The distribution of the major somatostatin receptor subtype, the sst2, was monitored in the hippocampus using immunofluorescence from 10 minutes to seven days after in vivo injection of the receptor agonist octreotide.Results: From 10 min to 3 h after agonist injection, intensity of receptor immunoreactivity gradually decreased in the molecular layer of dentate gyrus and in the strata oriens and radiatum of CA1. Concomitantly, in the granular and pyramidal layers, small spherical immunofluorescent particles became apparent in perikarya, shortly after agonist stimulation (i.e. 30 min, 60 min). After longer survival times (i.e. 3 h, 6 h, 24 h), immunolabeling was confined to larger, intensely-stained intracytoplasmic vesicles. From 48 h to 7 d after agonist injection, distribution and intensity of sst2 receptor immunoreactivity became similar to that of control animals. The sst2 receptor labeling extensively colocalized with TGN38 and syntaxin 6 after OCT injection. Colocalization with trans-Golgi markers was observed as soon as 1 h after OCT injection and still present 24 h after. By contrast, colocalization with the endoplasmic reticulum marker PDI and the cis-Golgi marker GM130 was never observed.Conclusions: Our results suggest that upon agonist stimulation, dendritic receptors are retrogradely transported to a trans-Golgi network domain enriched in the t-SNARE syntaxin-6 and TGN38 proteins before recycling.","PeriodicalId":520642,"journal":{"name":"Ideggyogyaszati szemle","volume":" ","pages":"136-9"},"PeriodicalIF":0.8,"publicationDate":"2007-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26679816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Projections of VIP/PHI neurons of the interstitial nucleus of Cajal in the rat. 大鼠Cajal间质核VIP/PHI神经元的投射。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Mariann Fodor, William Rostène, Anne Berod, Viktória Bencze, Miklós Palkovits

引用次数: 0

Protective action of snake venom Naja naja oxiana at spinal cord hemisection. 蛇毒对脊髓半切的保护作用。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Silva S Abrahamyan, Irina B Meliksetyan, Vergine A Chavushyan, Mery L Aloyan, John S Sarkissian

{"title":"Protective action of snake venom Naja naja oxiana at spinal cord hemisection.","authors":"Silva S Abrahamyan, Irina B Meliksetyan, Vergine A Chavushyan, Mery L Aloyan, John S Sarkissian","doi":"","DOIUrl":"","url":null,"abstract":"Based on data accumulated regarding the neuroprotective action of Proline-Rich-Peptide-1 (PRP-1, a fragment of neurophysin vasopressin associated hypothalamic glycoprotein consisting of 15 amino acid residues) on neurons survival and axons regeneration and taking into the account that LVV-Hemorphin-7 (LVV-H7, an opioid peptide, widely distributed in different cell types of various tissues of intact rats, including those of the nervous and immune systems) derived from the proteolytic processing of hemoglobin in response to adverse environmental and physiological conditions, possesses the anti-stressor properties, we used histochemistry, immunohistochemistry and electrophysiology to investigate the putative neuroprotective action of Central Asian Cobra Naja naja oxiana snake venom (NOX) on trauma-injured rats. ABC immunohistochemical method and histochemical method on detection of Ca2+-dependent acid phosphatase activity were used for the morpho-functional study. By recording the electrical activity of the signals from the single neurons in and below the SC injury place, NOX venom has been shown to result in the complete restoration of hypothalamic-spinal projections originated from ipsi- and contra-lateral PVN and SON to neurons of SC lumbar part. NOX prevented the scar formation, well observed two months after SC injury in the control rats, resulted in the regeneration of nerve fibers growing through the trauma region, survival of the PRP-1- and LVV-H7-immunoreactive (Ir) neurons, and increase of the PRP-1 - and LVV-H7-Ir nerve fibers and astrocytes in the SC lesion region. NOX was suggested to exert the neuroprotective effect, involving the PRP-1 and LVV-H7 in the underlying mechanism of neuronal recovery.","PeriodicalId":520642,"journal":{"name":"Ideggyogyaszati szemle","volume":" ","pages":"148-53"},"PeriodicalIF":0.8,"publicationDate":"2007-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26679820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular architecture of the cannabinoid signaling system in the core of the nucleus accumbens. 伏隔核核心大麻素信号系统的分子结构。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

Ferenc Mátyás, Masahiko Watanabe, Ken Mackie, István Katona, Tamás F Freund

{"title":"Molecular architecture of the cannabinoid signaling system in the core of the nucleus accumbens.","authors":"Ferenc Mátyás, Masahiko Watanabe, Ken Mackie, István Katona, Tamás F Freund","doi":"","DOIUrl":"","url":null,"abstract":"Several abused drugs are known to alter glutamatergic signaling in reward pathways of the brain, and these plastic changes may contribute to the establishment of addiction-related behaviour. Glutamatergic synapses of the prefrontal cortical projections to the nucleus accumbens (nAcb)--which are suggested to be under endocannabinoid (eCB) control - play a central role in the addiction process. The most abundant eCB in the brain is 2-arachi-donoyl-glycerol (2-AG). It is synthesized by diacylglycerol lipase alpha (DGL-alpha), and exerts its action via type 1 cannabinoid receptors (CB1). However, the precise localization of DGL-alpha and CB1 - i.e. the sites of synthesis and action of 2AG - is still unknown. At the light microscopic level, immunocytochemistry revealed a granular pattern of DGL-alpha distribution in the core of the nAcb. Electron microscopic analysis confirmed that these granules corresponded to the heads of dendritic spines. On the other hand, presynaptic axon terminals forming excitatory synapses on these spineheads were found to express CB1 receptors. Our results demonstrate that the molecular constituents for a retrograde endocannabinoid control of glutamatergic transmission are available in the core of the nAcb, and their relative subcellular location is consistent with a role of 2-AG in addiction-related plasticity of cortical excitatory synapses in this reward area.","PeriodicalId":520642,"journal":{"name":"Ideggyogyaszati szemle","volume":" ","pages":"187-91"},"PeriodicalIF":0.8,"publicationDate":"2007-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26680690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue dedicated to the memory of Dr. Mariann Fodor. 纪念玛丽安·福多博士的特刊。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

引用次数: 0

Effects of ketamine on the developing central nervous system. 氯胺酮对发育中的中枢神经系统的影响。

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Ideggyogyaszati szemle Pub Date : 2007-03-30

László Vutskits, Eduardo Gascon, József Zoltán Kiss

引用次数: 0