Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia最新文献

{"title":"¹³C-methacetin breath test in the evaluation of disease severity in patients with liver cirrhosis.","authors":"Jana Vranova,&nbsp;Miluse Hendrichova,&nbsp;Hana Kolarova,&nbsp;Karolina Kratka,&nbsp;Jozef Rosina,&nbsp;Jiri Horak","doi":"10.5507/bp.2012.114","DOIUrl":"https://doi.org/10.5507/bp.2012.114","url":null,"abstract":"<p><strong>Aims: </strong>The non-invasive (13)C-methacetin ((13)C-MBT) breath test has been proposed as a measure of metabolic liver function that improves the diagnostic efficacy of serologic and biochemical tests in assessing hepatic functional capacity and liver disease severity, The goal of this study was to establish the clinical utility of this test in quantifying hepatic metabolic function in patients with liver cirrhosis of varying severity and to compare (13)C-MBT measurements with the AST/ALT ratio, APRI score, and other routine liver tests.</p><p><strong>Methods: </strong>Routine liver function tests including serum bilirubin, aspartate aminotransferase activity (AST), alanine aminotransferase (ALT), AST/ALT ratio, the APRI score, the percentage of dose rate (PDR) and cumulative percentage of dose rate (CPDR) of the (13)C-MBT were evaluated in 52 cirrhotic patients of alcohol etiology (Child-Pugh A/B/C 10/28/14) and 37 healthy controls.</p><p><strong>Results: </strong>The (13)C-MBT differed significantly between healthy controls and cirrhotic patients at all time intervals measured. It also proved the ability to differentiate patients with liver cirrhosis based on severity of hepatic impairment corresponding to the Child-Pugh classification A vs. B vs. C. The ROC curve analysis suggested that the best prediction is provided by time intervals between the 10th - 20th or 10th - 40th minute of PDR.</p><p><strong>Conclusions: </strong>The (13)C-MBT offers a reliable means for quantification of hepatic metabolic function over the complete range of functional liver impairment. It is non-invasive, easy to perform and completely safe.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"392-400"},"PeriodicalIF":0.9,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40243065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of promoter methylation of GATA4 gene in epithelial ovarian cancer.","authors":"Marcela Chmelarova,&nbsp;Eva Dvorakova,&nbsp;Jiri Spacek,&nbsp;Jan Laco,&nbsp;Vladimir Palicka","doi":"10.5507/bp.2013.079","DOIUrl":"https://doi.org/10.5507/bp.2013.079","url":null,"abstract":"<p><strong>Aims: </strong>Ovarian cancer is the most lethal gynecological malignancy, with typically late diagnosis. Altered DNA methylation of tumor suppressor gene promoters probably plays a relevant role in ovarian carcinogenesis and frequently occurs as an early event in the development of different types of cancer including ovarian carcinoma. GATA4 methylation has been reported in a variety of human cancers. The aim of this study was to investigate promoter methylation of the GATA4 gene in ovarian cancer by comparison with that in normal ovarian tissue.</p><p><strong>Methods: </strong>To search for promoter methylation of the GATA4 gene we used MSP (methylation-specific PCR) to compare the methylation status in 67 tissue samples of ovarian cancer with that in 40 control samples.</p><p><strong>Results: </strong>In our study, methylation-specific PCR revealed GATA4 promoter methylation in 21 of 67 specimens with ovarian cancer (31.3%), and in none of the control ovarian tissue samples.</p><p><strong>Conclusion: </strong>These results confirm that methylation in the GATA4 promoter region could play an important role in ovarian carcinogenesis, and show new loci which are highly methylated only in ovarian cancer samples and which are associated predominantly with the endometrioid type of ovarian carcinoma.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"294-7"},"PeriodicalIF":0.9,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40253032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The treatment of severe pain in Canada should be followed.","authors":"Roman Hudec","doi":"10.5507/bp.2012.113","DOIUrl":"https://doi.org/10.5507/bp.2012.113","url":null,"abstract":"To the editor: H.W. Striebel in his book “Therapy of chronic pain“ wrote: “We all can be excused if we cannot cure a patient, but not if we don’t try to relieve suffering and pain. The epitaph of the ancient medical doctor Galenos was: “Divinum est sedare dolorem.“ (Relieving pain is a labor of God). In the figure below are shown the trends in morphine use, still considered a standard in the opioid analgesics group. Countries were chosen as top consumption leaders (Canada, USA), as well as their historic connections.The Czechoslovakia was one state between 1918 and 1993 and Slovakia was until 1918 a part of the Hungarian kingdom. Differences in trends beween countries are very clear. Some may say that this is only consumption of morphine, that differences in analgesic use may arise because alternative analgesics are preferred. However e.g. according to the INCB yearbook 2009 Canada and USA were in the 1 and 2nd place wrt consumption of oxycodone worldwide, and the situation is similar for use of fentanyl (2nd and 6th place worldwide resp.). In contrast our countries (Czech republic, Slovakia and Hungary) were in the 24th, 23rd and 22nd place resp. for fentanyl consumption and in the 19th, 30th and 37th place for oxycodone consumption worlwide. Variations in medical training, regulatory requirements and drug pricing can stimulate different prescribing decisions. The data from the Slovak State Institute for Drug Control were used in my observation and I have calculated the percentage of these strong opioids on the whole consumption of opioid analgesics (ATC group N02A). In the year 2000 morphine constituted in Slovakia 6.5%, fentanyl 6% and oxycodone was not available. In the year 2009 the situation was different. Morphine constituted only 1%, fentanyl more than 17% and oxycodone 11% – the shift to other opioids is clear. In our previously published manuscripts we brought this problem to attention and we can still say that especially severe pain remains insufficiently treated in Slovakia as it is improbable that we have less pain. Progress in Canada (number 1 worldwide) as well as USA (consumption of morphine doubled over the observed years) should be followed. Of Central European countries the biggest consumption was in the Czech republic. One factor contributing to the inadequate treatment of pain is the laws and regulations that impede effective pain management. Opioids are our most powerful analgesics, but politics, prejudice, and our continuing ignorance still impede optimum prescribing. There is still some reluctance among doctors to prescribe opioids, even for cancer pain, and reluctance of patients and their families to accept opioid medication.","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"385"},"PeriodicalIF":0.9,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40220322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathophysiology of endothelial function in pregnancy and the usefulness of endothelial markers.","authors":"Ludek Slavik,&nbsp;Jana Prochazkova,&nbsp;Martin Prochazka,&nbsp;Ondrej Simetka,&nbsp;Antonin Hlusi,&nbsp;Jana Ulehlova","doi":"10.5507/bp.2011.031","DOIUrl":"https://doi.org/10.5507/bp.2011.031","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to assess coagulation markers of endothelial damage and examine new markers of endothelial activation such as matrix metalloproteinases (MMPs) in a group of healthy pregnant women. Matrix metalloproteinase (MMP)-2, in particular, plays a major role in the degradation of the extracellular matrix confirming its essential function in both the survival (angiogenesis) and death of endothelial cells. Detection of specific coagulation factors, mainly released from the vascular endothelium such as vWF, sTM (soluble thrombomodulin) and ePCR (endothelial protein C receptor) and factors dependent on endothelial activation such as t-PA and PAI-1, could provide information on possible endothelial dysfunction and help differentiate pregnant patients with an altered thrombotic state.</p><p><strong>Methods: </strong>Healthy pregnant women underwent complete assessment for endothelial damage (as vWF, vWF activity, sTM, ePCR, EMP, MMP-2, MMP-9 and TIMP-2) using the ELISA and other methods.</p><p><strong>Results and conclusions: </strong>The results show that endothelial activation during pregnancy is different from that in other pathological conditions involving endothelial damage and typically characterized by higher levels of both coagulation endothelial markers and MMPs. In pregnancy, changes in extracellular matrix composition and matrix metalloproteinase activity also occur and promote vascular remodeling but, only in the uterus. Predisposing risk factors for epithelial dysfunction, and vascular mediators associated with vascular remodeling must be assessed from concentrations in whole blood. The levels of MMPs are not increased in the circulation and the local situation in the uterus cannot be monitored this way. However, MMP-2 processes and modulates the functions of many other vasoactive and pro-inflammatory molecules including adrenomedullin, big endothelin-1, calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, galectin-3, IGFBP-3, IL-1 Beta, S100A8, and S100A9. These molecules represent new potential molecular markers of endothelial damage during pregnancy.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"333-7"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30460768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychoeducation for psychotic patients.","authors":"Jan Prasko,&nbsp;Kristyna Vrbova,&nbsp;Klara Latalova,&nbsp;Barbora Mainerova","doi":"10.5507/bp.2011.008","DOIUrl":"https://doi.org/10.5507/bp.2011.008","url":null,"abstract":"<p><strong>Background: </strong>Psychoeducation programs have been shown in the last two decades to significantly improve the post-hospital discharge care of individuals with schizophrenia by demonstrated effects on rehospitalization rates, compliance with medication and knowledge. The benefits of psychoeducation can be sustained for up to seven years although most studies have not shown such enduring benefit. Psychoeducation is a type of psychotherapeutic support that aims to provide broad and adequate information on psychotic disorders for both patients and their families.</p><p><strong>Method: </strong>The purpose of the group psychoeducational program at the Psychiatric clinic in Olomouc is to familiarize patients with the core of the disorder, explain the part played by pharmacotherapy, teach recognition of the warning signs of relapse, to advise on communication problems within families, and finally the training of social skills.</p><p><strong>Results: </strong>Patients perceive the program as meaningful and helpful in better understanding their lives.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"385-95"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30461777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endosonography versus endoscopic retrograde cholangiopancreatography in diagnosing extrahepatic biliary obstruction.","authors":"Igor Tozzi di Angelo,&nbsp;Vlastimil Prochazka,&nbsp;Martin Holinka,&nbsp;Jana Zapletalova","doi":"10.5507/bp.2011.044","DOIUrl":"https://doi.org/10.5507/bp.2011.044","url":null,"abstract":"<p><strong>Objective: </strong>Endosonography (EUS) performed prior to endoscopic retrograde cholangiopancreatography (ERCP) or surgery in patients with a low to moderate probability of choledocholithiasis can reduce morbidity, mortality and costs.</p><p><strong>Aims: </strong>This study aimed at evaluating the sensitivity and specificity of EUS and transabdominal ultrasonography (TUS) compared to ERCP in a cohort of 100 patients diagnosed with extrahepatic biliary obstruction.</p><p><strong>Methods: </strong>There were four working groups. The first group processed the initial data including history, physical examination and assessment of laboratory markers of cholestasis. The second group examined TUS. The third group examined the pancreas and biliary tree using EUS, always prior to ERCP. The fourth working group carried out ERCP.</p><p><strong>Results: </strong>The sensitivity and accuracy of EUS in the diagnosis of common bile duct dilatation were 84% and 83%, respectively, i.e. significantly higher (p=0.0001) than TUS (46% and 66%, respectively). The specificities of methods (82%, 91%) were not significantly different (p=0.218). The sensitivity and accuracy of EUS in the diagnosis of pathological content of the common bile duct were 88% and 92%, respectively, i.e. significantly higher (p<0.0001) than TUS (33% and 64%). The specificities of the methods (96%, 94%) were not significantly different (p=0.641). The incidence of choledocholithiasis in the high-risk group was significantly higher than in the moderate-risk group (p=0.012).</p><p><strong>Conclusion: </strong>Radial EUS is a method with high sensitivity, specificity, positive and negative predictive values. It should therefore be preferred to ERCP in patients with low or moderate risk of choledocholithiasis.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"339-46"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30461771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age, body mass index and severity of primary chronic venous disease.","authors":"Dalibor Musil,&nbsp;Marketa Kaletova,&nbsp;Jiri Herman","doi":"10.5507/bp.2011.054","DOIUrl":"https://doi.org/10.5507/bp.2011.054","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;The aim of this cross-sectional prospective study was investigate whether there is a correlation between age, BMI and severity of chronic venous disease (CVD), evaluated clinically (CEAP classification) and anatomically (extent of the epifascial venous reflux).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;213 patients, 65 males (30.5%, mean age 45.1 ± 13.9 years) and 148 females (69.5%, mean age 47.5 ± 13.5 years) were divided into three age categories: 18-40 years (40.8%), 41-74 years (56.3%) and ≥75years (2.8%). BMI was classified as normal weight (18.5 - &lt;25 kg/m(2)), overweight (25 - &lt;30 kg/m(2)) and obesity (≥30 kg/m(2)). Clinical examination of the lower limbs assessed presence and severity of venous signs as included in the CEAP classification. BMI was calculated. Anatomical extent of CVD was described as the number of segments of the superficial and perforating veins with documented reflux by duplex imaging.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Median age increased the number of insufficient venous segments (1 insufficient venous segment - median age 41.0 years, 5 insufficient venous segments - median age 51.0 years). The frequency of reflux in the superficial and perforating veins significantly increased with age (p&lt;0.05). A statistically significant correlation was also found between age and the CEAP classification (p&lt;0.01). This was more significant than the correlation between age and number of insufficient venous segments. In the whole group and in women the Spearman's correlation analysis revealed only a weak positive correlation between BMI and reflux in the superficial veins (r=0.145 respectively r=0.264) (p&lt;0.05). No correlation was found in men (r=0.091). Weak positive correlation between BMI and stage of venous insufficiency (CEAP classification) was demonstrated for the whole group of patients (r=0.229, p&lt;0.01), for women (r=0.293, p&lt;0.05) and for men (r=0.245, p&lt;0.01). Multiple linear regression showed age (p&lt;0.0001) and BMI (p=0.049) as significant predictors of clinical grade according to the CEAP classification and the CEAP clinical class (p&lt;0.0001) as a significant predictor of extent of the epifascial venous reflux.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The study confirmed the relationship between age, clinical (CEAP clinical class) and pathophysiological (extent of the venous reflux) severity of CVD Older age means an increased number of insufficient venous segments and increased risk of the clinical progression of CVD from varicose veins to chronic venous insufficiency (C(3)-C(6), trophic skin changes and venous ulcers). Our results support the BMI, in term of frequency of venous reflux, as a risk factor in the whole group of patients but only in women but not in men. Multiple linear regression showed BMI together with age as significant predictors of clinical grade of CVD (p&lt;0.05) according to the CEAP classification. As regards the influence of BMI on clinical severity/grade of CVD (CEAP), the results of our stu","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"367-71"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30461774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute interstitial pneumonia (Hamman-Rich syndrome) in idiopathic pulmonary fibrosis and bronchoalveolar carcinoma: a case report.","authors":"Jiri Plasek,&nbsp;Jana Dvorackova,&nbsp;Jan Jahoda,&nbsp;Kristina Trulikova,&nbsp;Radka Mokosova,&nbsp;Tomas Danek,&nbsp;Vladimir Hrabovsky,&nbsp;Arnost Martinek","doi":"10.5507/bp.2011.039","DOIUrl":"https://doi.org/10.5507/bp.2011.039","url":null,"abstract":"<p><strong>Aim: </strong>Acute interstitial pneumonia is characterized by rapid progressive dyspnoea degenerating into respiratory failure requiring mechanical ventilation. Acute interstitial pneumonia (AIP) and idiopathic pulmonary fibrosis (IPF) are separate clinic/pathological entities although overlap may be present. It is well-known that patients with IPF have increased risk of lung carcinoma; Adenocarcinoma in connection with IPF is less common. Moreover the subtype of adenocarcinoma, diffuse bronchoalveolar carcinoma has not yet been described.</p><p><strong>Case report: </strong>We report the case of 45 yr old former hockey player with increased bilateral reticular shadowing on chest radiograph, dyspnoea, velcro-like crackles, restrictive respiratory disease and mixed high-resolution computed tomography finding. During brief in-patient treatment the patient developed acute respiratory failure accompanied by multiorgan failure and disseminated coagulopathy. Deterioration of the microcirculation was followed by loss of peripheral vascular resistance, which was irreversible even with normalization of the blood gases achieved by extracorporeal membrane oxygenation. At autopsy, bronchoalveolar carcinoma in usual interstitial pneumonia (UIP) combined with areas of alveolar damage with hyaline membranes was found.</p><p><strong>Conclusion: </strong>This case alerts clinicians to unusual idiopathic pulmonary fibrosis manifestations and its complications. Close collaboration between clinicians, pathologists and laboratory physicians is highly recommended for early diagnosis and appropriate treatment.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"403-7"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30461728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystic adventitial disease of the popliteal artery: report on three cases and review of the literature.","authors":"Petr Drac,&nbsp;Martin Köcher,&nbsp;Petr Utikal,&nbsp;Marie Cerna,&nbsp;Jiri Kozak,&nbsp;Petr Bachleda","doi":"10.5507/bp.2011.065","DOIUrl":"https://doi.org/10.5507/bp.2011.065","url":null,"abstract":"<p><strong>Background: </strong>During the past nine years three cases of cystic adventitial disease of the popliteal artery have been diagnosed and treated in our medical institution. Different approaches were used in the treatment in all these cases. The purpose of this report is to summarise the current knowledge of the etiology, presentation, diagnostics and treatment of this condition, with the addition of new cases.</p><p><strong>Methods: </strong>Information about three new cases is presented and discussed together with that from the relevant publications obtained from the Pubmed database. Results. In the first case resection with synthetic graft interposition was used. Nine years after the surgery the patient is without any signs of recurrence, but he experienced local thrombolysis of the occluded graft and repeated PTA of hemodynamically significant anastomotic stenoses. The second case treated with US-guided aspiration has demanded repeated reinterventions due to recurrence; nevertheless, the result is satisfactory. In the last instance, the cyst was evacuated and excised. Six months after the surgery the patient is symptom-free and without signs of recurrence.</p><p><strong>Conclusions: </strong>CAD of the popliteal artery is a rare vascular condition. However, it must be considered in the differential diagnosis, especially in middle-aged male patients without evidence of atherosclerotic disease in whom intermittent claudication has developed suddenly with a rapid progression or with fluctuation in severity. Duplex ultrasound and MRA are the the best diagnostic methods. Based on the existing knowledge, the treatment of choice is surgery (either evacuation with the removal of the cystic wall or resection and grafting).</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"309-21"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30460765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ceruloplasmin plasma levels in patients with severe forms of herpes infection.","authors":"Irina Novikova,&nbsp;Maria Zlotnikova","doi":"10.5507/bp.2011.051","DOIUrl":"https://doi.org/10.5507/bp.2011.051","url":null,"abstract":"<p><strong>Background: </strong>Research interest in ceruloplasmin (CP) has significantly increased in recent years owing to new discoveries of its properties including anti-inflammatory and anti-oxidant effects. Data on CP blood plasma level in patients with herpetic infection caused by herpes simplex virus are scarce and often contradictory. Most point to a reduction of CP in the blood plasma of patients during the exacerbation of the disease with gradually return to normal values after treatment. There is evidence of an increase in CP levels during the acute period of CRHI (chronic recurrent herpes infection) and decrease in remission.</p><p><strong>Materials and methods: </strong>The content of ceruloplasmin in blood plasma was determined by immunoturbidimetry using the test-systems \"Spinreact\" (Spain) and biochemical analyzer \"Architect C8000\".</p><p><strong>Results: </strong>We found that in patients with severe forms of chronic recurrent herpes infection in the exacerbation period, CP levels were increased by approximately 35% relative to the control values. However in the remission period in 80% of patients, CP concentrations remained elevated and in some patients the CP level was even increased in comparison with values in the exacerbation. Such dynamics of CP were not caused by the exacerbation of concomitant diseases.</p><p><strong>Conclusion: </strong>The increased CP levels in the remission period in patients with severe forms of chronic recurrent herpes infection may have been caused by its effect as an endogenous antioxidant. No correlations of CP levels with other laboratory signs of acute inflammation were found but identified was a relation to free radical activity.</p>","PeriodicalId":520569,"journal":{"name":"Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia","volume":" ","pages":"361-6"},"PeriodicalIF":0.9,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30461773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}