Jarosław P Chmielewski, Barbara Gworek, Halina Król, Roman Starz, Sławomir Józef Krzebietke, Magdalena Florek-Łuszczki
{"title":"Health and environmental risk assessment of berry fruits contaminated with pesticide residues, including soil ecosystem exposure.","authors":"Jarosław P Chmielewski, Barbara Gworek, Halina Król, Roman Starz, Sławomir Józef Krzebietke, Magdalena Florek-Łuszczki","doi":"10.26444/aaem/214156","DOIUrl":"10.26444/aaem/214156","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Pesticides, also known as plant protection products, are used to control pests, plant diseases, and weeds during crop cultivation and post-harvest handling. The aim of the study is evaluation of the health and environmental risks associated with pesticide residues in berry fruits (strawberries, raspberries, and blueberries) available in Poland in 2022.</p><p><strong>Material and methods: </strong>The study is based on the results of officially controlled, laboratory analyses of pesticide residues in berry fruits available on the Polish market in 2022, data on fruit consumption, and literature data concerning acceptable daily intake values (ADI). Estimated daily intake (EDI) and hazard quotients (HQ) were calculated to assess the chronic dietary risk. In addition, a screening-level environmental risk assessment was carried out for the same active substances, focusing on the soil compartment as the main sink of pesticide residues.</p><p><strong>Results: </strong>The health risk assessment showed that all calculated EDI, HQ, and HI values for pesticide residues in strawberries, raspberries, and blueberries consumed in Poland in 2022 were several orders of magnitude below the threshold of concern (HQ < 1). The environmental risk assessment indicated that most fungicides (boscalid, cyprodinil, fludioxonil, fluopyram) posed low risk to soil organisms (RQ < 1), even under conservative scenarios. However, acetamiprid exceeded the risk threshold at higher assumed soil concentrations (MEC = 0.10 mg/kg), suggesting potential ecological concern for sensitive soil invertebrates, such as collembolans.</p><p><strong>Conclusions: </strong>The study showed that pesticide residues in strawberries, raspberries, and blueberries available in Poland in 2022 did not pose a chronic health risk to consumers. Environmental risk assessment indicated low risk for most fungicides; however, acetamiprid exceeded the safety threshold under higher soil contamination scenarios, suggesting potential concern for soil invertebrates.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"98-104"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Małgorzata Staszczyk, Małgorzata Jamka-Kasprzyk, Beata Stepańczak-Cienkosz, Iwona Szymiec, Jerzy Kuliński, Anna Jurczak, Iwona Gregorczyk-Maga
{"title":"Effects of a <i>Lactobacillus salivarius</i> probiotic short-term intervention on <i>S. mutans</i>, <i>Lactobacillus</i> spp. and <i>C. albicans</i> - a randomized pilot study with pre-school children.","authors":"Małgorzata Staszczyk, Małgorzata Jamka-Kasprzyk, Beata Stepańczak-Cienkosz, Iwona Szymiec, Jerzy Kuliński, Anna Jurczak, Iwona Gregorczyk-Maga","doi":"10.26444/aaem/211598","DOIUrl":"10.26444/aaem/211598","url":null,"abstract":"<p><strong>Introduction and objective: </strong>The aim of the study is to evaluate the effect of short-term, oral supplementation of non-viable <i>Lactobacillus salivarius</i> (HM6 Paradens) on the number of caries-related microorganisms in the supragingival plaque biofilm of pre-school children.</p><p><strong>Material and methods: </strong>The study is prospective, randomized and observational in design performed on 2 parallel groups (test and control) of 72 childrenaged between 3 - 6 years, with or without early childhood caries (ECC). The primary outcomes measured are changes in <i>S. mutans</i> counts (number of colony-forming units - CFU) and the percent of the total viable counts) in dental plaque biofilm within the groups between baseline and 2-weeks follow-up, while secondary outcomes are changes in <i>Lactobacilli</i> spp. and <i>C. albicans</i> counts.</p><p><strong>Results: </strong>For analysis performed only on the children with detectable bacterial counts at baseline, the reduction in the level of <i>S. mutans</i> increased in the control group, and decreased in the probiotic group, <i>Lactobacillus</i> spp. increased in the probiotic group and decreased in the control group, and for <i>C. albicans</i>, the level decreased in both the control and probiotic groups after 2 weeks.</p><p><strong>Conclusions: </strong>The presence of <i>L.salivarius</i> (HM6 Paradens) may modify the interactions between <i>S. mutans</i> and <i>Lactobacillus</i> spp., which could support microbiological homeostasis and have a beneficial impact on ECC prevention.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"31-38"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of high-intensity interval exercise on attentional network function in youth athletes.","authors":"Lian Wang, Mariusz Lipowski","doi":"10.26444/aaem/207244","DOIUrl":"10.26444/aaem/207244","url":null,"abstract":"<p><strong>Introduction and objective: </strong>The aim of the study is to explore the impact of high-intensity interval exercise (HIIE) on the attentional network function in youth athletes.</p><p><strong>Material and methods: </strong>A total of 44 youth athletes (mean age: 15.2 ± 1.1 years) were randomly assigned to either an experimental group (n = 22) or a control group (n = 22). The experimental group engaged in a 30-minute HIIE session, while the control group watched a 30-minute documentary. Attentional functions -including alerting, orienting, and executive control - were assessed using the Attention Network Test (ANT) immediately before and after the interventions.</p><p><strong>Results: </strong>Post-intervention, the experimental group exhibited a significant improvement in executive control, with reaction times decreasing from 102 ± 15 ms to 89 ± 12 ms (p < 0.01). In contrast, the control group's reaction times remained relatively unchanged (pre-test: 101 ± 14 ms; post-test: 100 ± 13 ms; p = 0.45). No significant differences were observed between groups in the alerting effect (experimental: pre-test 35 ± 8 ms, post-test 34 ± 7 ms; control: pre-test 36 ± 9 ms, post-test 35 ± 8 ms), or the orienting effect (experimental: pre-test 42 ± 10 ms, post-test 41 ± 9 ms; control: pre-test 43 ± 11 ms, post-test 42 ± 10 ms).</p><p><strong>Conclusions: </strong>HIIE significantly improves executive control network function in youth athletes, with limited effects on alerting and orienting networks. These findings suggest that HIIE can enhance cognitive functions, particularly executive control, which can inform the development of optimized training programmes for youth athletes.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"118-124"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Piotr Sajdak, Oskar Kwiatkowski, Hanna Czajka, Nikola Król, Aleksandra Łoś, Aleksandra Krużel, Oktawia Wojtuń, Seweryn Ziajor, Klaudia Jakubowska, Piotr Dreher
{"title":"Vaccination status and attitudes of Ukrainians in Poland towards mandatory vaccinations.","authors":"Piotr Sajdak, Oskar Kwiatkowski, Hanna Czajka, Nikola Król, Aleksandra Łoś, Aleksandra Krużel, Oktawia Wojtuń, Seweryn Ziajor, Klaudia Jakubowska, Piotr Dreher","doi":"10.26444/aaem/207246","DOIUrl":"10.26444/aaem/207246","url":null,"abstract":"<p><strong>Introduction and objective: </strong>The study examines the vaccination status and attitudes of Ukrainian refugees in Poland, focusing on factors influencing decisions regarding mandatory vaccinations. Given the ongoing migration and geopolitical instability, understanding these determinants is crucial for improving immunization coverage and mitigating public health risks.</p><p><strong>Material and methods: </strong>Data were collected from 102 Ukrainian refugee participants in the Rzeszów area using a structured survey. The questionnaire included demographic questions and inquiries about vaccination practices, reasons for incomplete immunization, and attitudes toward vaccination. Statistical analysis was conducted to identify significant correlations.</p><p><strong>Results: </strong>The study found that 55.88% of participants reported full vaccination of their children, while 42.16% had partial vaccination, and less than 2% were unvaccinated. The most common barriers to vaccination were limited access to vaccines (40%) and mistrust in vaccine formulations (37.78%). Additionally, statistical analysis revealed a significant correlation between the COVID-19 pandemic and vaccination uptake (p < 0.05).</p><p><strong>Conclusions: </strong>The findings emphasize the need for targeted interventions to address vaccine hesitancy and logistical challenges among Ukrainian refugees. Strengthening cooperation between Ukraine and Poland is crucial for enhancing immunization coverage. Despite broader vaccine availability in Poland, hesitancy remains an issue, necessitating educational initiatives and policy adjustments.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"125-129"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effectiveness of ChatGPT in simplifying information for Polish patients on cervical cancer screening using advanced prompting.","authors":"Joanna Gotlib-Małkowska, Magdalena Dębska","doi":"10.26444/aaem/204249","DOIUrl":"10.26444/aaem/204249","url":null,"abstract":"<p><strong>Introduction and objective: </strong>ChatGPT can generate reliable medical information in gynaecology and obstetrics,but the content is often difficult to understand for patients with lower educational levels. The aim of the study is to evaluate the impact of Audience Persona Prompting on the simplification and readability of ChatGPT-generated medical information on cervical cancer screening (MICC_GPT) in Polish.</p><p><strong>Material and methods: </strong>392 MICC_GPT were analyzed, with 196 generated using Zero-Shot Prompting (STANDARD) and 196 generated using Audience Persona Prompting (EASY). The Audience Persona prompts included instructions to simplify the content: 'Explain as if to an average Polish woman with only primary education' (8 years of formal schooling). Readability was assessed using 24 objective linguistic indicators available at Jasnopis.pl. Statistica 13 (StatSoft, Poliand), the Brunner-Munzel test, p < 0.05.</p><p><strong>Results: </strong>The average difficulty level of STANDARD output was 5.32 (at least 15 years of formal education), while EASY output averaged 4.15 (12 years of formal education). Of the 24 indicators, 21 showed statistically significant improvements in the simplification of EASY output (p < 0.05). While ChatGPT significantly simplified MICC_GPT, the readability levels remained too high for patients with only primary education.</p><p><strong>Conclusions: </strong>ChatGPT shows promise in tailoring medical information on cervical cancer (CC) screening for the needs of Polish patients with varying educational backgrounds, with the use of advanced prompt engineering techniques. However, further research is required to refine prompt engineering methods and develop effective strategies for generating information on cervical cancer screening that is accessible to individuals with only primary education.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"46-52"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guoping Qian, Piotr Aschenbrenner, Jinwei Zhao, Tong Han, Yu Wu, Hongli Yu, Zbigniew Ossowski
{"title":"Non-pharmacological interventions for gait rehabilitation in children with neurodisability - A bibliometric and visualization analysis.","authors":"Guoping Qian, Piotr Aschenbrenner, Jinwei Zhao, Tong Han, Yu Wu, Hongli Yu, Zbigniew Ossowski","doi":"10.26444/aaem/212385","DOIUrl":"10.26444/aaem/212385","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Gait impairments significantly impact the daily active participation and quality of life in children with neurodisabilities. Non-pharmacological interventions (NPIs) have demonstrated positive outcomes in gait rehabilitation, although comprehensive analyses that map the global research landscape in this domain are limited. The aim of the bibliometric review is to systematically explore and visualize global research trends, identify hotspots, and highlight emerging themes related to NPIs for gait rehabilitation in children with neurodisability. The literature coverage spans 1993-2024.</p><p><strong>Review methods: </strong>Literature was collected from the Web of Science Core Collection using the keywords \"cerebral palsy,\" \"rehabilitation,\" and \"gait,\" and was systematically analyzed.</p><p><strong>Brief description of the state of knowledge: </strong>A total of 455 relevant documents were included. The number of studies has steadily increased from 1993 to 2024 in this field. The United States leads in terms of publication volume and cumulative citations, with Developmental Medicine & Child Neurology published the highest number of papers in this area. Emerging technologies such as exoskeletons, virtual reality and robot-assisted gait training have become research hotspots.</p><p><strong>Results: </strong>The findings of the study provide researchers and clinicians with a clear, objective understanding of the current state and future directions in this important area of research.</p><p><strong>Summary: </strong>The study visually represented research trends on the NPIs in gait rehabilitation for children with neurodisability, revealed emerging patterns of research and areas of focus.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"12-23"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleksandra Marta Spychał, Barbara Oczko-Grzesik, Katarzyna Pancer, Barbara Sobala-Szczygieł, Maciej Piasecki, Karol Jerzy Żmudka, Jerzy Jaroszewicz
{"title":"First documented case of Zika virus infection in a Polish patient - diagnostic traps. Case report.","authors":"Aleksandra Marta Spychał, Barbara Oczko-Grzesik, Katarzyna Pancer, Barbara Sobala-Szczygieł, Maciej Piasecki, Karol Jerzy Żmudka, Jerzy Jaroszewicz","doi":"10.26444/aaem/205174","DOIUrl":"10.26444/aaem/205174","url":null,"abstract":"<p><p>Zika virus (ZIKV), a mosquito-borne virus of the Flaviviridae family, has been reported in 89 countries. While ZIKV is not endemic in Poland, travel to regions with Aedes mosquitoes prevalence, presents a risk. This case report describes Poland's first ZIKV case in a patient with past Dengue virus (DENV) infection, following travel with his wife in 2016 to the Dominican Republic. Symptoms included fever, maculopapular rash, muscle pain, enlarged cervical lymph nodes, and lumbar pain. Hospital tests confirmed ZIKV, as well as possible simultaneous DENV infection. Further RT-PCR and serology tests were performed to exclude cross-reactivity. After 12 weeks, ZIKV RNA was detected in seminal fluid. Elevated IgG levels indicated prior DENV infection, while Dengue virus' antigen of non-structural protein 1 (NS1) antigen tests ruled out acute DENV. These findings impacted the couple's reproductive plans due to potential ZIKV transmission and patient's risk of secondary infection.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 1","pages":"143-147"},"PeriodicalIF":1.2,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147577559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karol Paradowski, Małgorzata Goździewska, Mirosław J Jarosz, Małgorzata Polz-Dacewicz
{"title":"Salivary EBV DNA in periodontitis - analysis of clinicopathological and serological parameters.","authors":"Karol Paradowski, Małgorzata Goździewska, Mirosław J Jarosz, Małgorzata Polz-Dacewicz","doi":"10.26444/aaem/208171","DOIUrl":"https://doi.org/10.26444/aaem/208171","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Oral diseases affect about 3.5 billion people worldwide, with periodontitis being a major concern due to its potential to cause tooth loss. The Epstein-Barr virus (EBV), which infects most of the global population, is linked to several cancers and may contribute to periodontal disease. Growing evidence suggests a significant role of the EBV in the etiopathogenesis of periodontitis. The aim of the study was to assess the EBV load in saliva and crevice-gingival fluid (CGF) in patients with periodontitis. The study also took into account the clinical stage of periodontitis and the OHI.</p><p><strong>Material and methods: </strong>The study included a total of 60 patients (36 women and 24 men) treated for periodontitis at the Dental Centre in Lublin. The research used crevice-gingival fluid (CGF), saliva and sera. EBV DNA was detected by PCR in the CGF and saliva, and anti-EBV antibodies were detected in the serum (ELISA test).</p><p><strong>Results: </strong>EBV DNA was detected in 39.5% of patients vs. 16.7% in healthy people. Both anti-EBNA and anti-EBVCA antibodies level was statistically significantly higher among patients with periodontitis compared to the control group. The obtained results encourage further in-depth research in this area.</p><p><strong>Conclusions: </strong>In more advanced stages of periodontitis, higher levels of viral load and higher levels of anti-EBV antibodies were observed. Moreover, the higher the level of antibodies in the serum, the higher the level of EBV viremia was detected. Saliva is a good clinical diagnostic material for EBV in oral diseases.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"32 4","pages":"492-496"},"PeriodicalIF":1.2,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Elucidating environmental reservoir of antimicrobial resistance - a phenotypic characterization of gut microbiota from aquatic coleoptera in a low-anthropogenic impact zone.","authors":"Figen Orhan, Mehmet Bektaş, Özlem Bariş","doi":"10.26444/aaem/210421","DOIUrl":"https://doi.org/10.26444/aaem/210421","url":null,"abstract":"<p><strong>Introduction and objective: </strong>This study investigated the antibiotic resistance of bacterial isolates obtained from the gut microbiota of certain insects (Coleoptera: Hydrophilidae and Helophoridae), which were collected from aquatic areas in Erzurum Province, Türkiye. This area is characterised by a low level of human impact, thereby providing a unique opportunity to investigate the baseline microbial diversity and ecological roles within relatively pristine aquatic environments.</p><p><strong>Material and methods: </strong>The antimicrobial susceptibility of the isolates was assessed using disc diffusion and minimum inhibitory concentration (MIC) methods. The analysis encompassed 30 Gram-negative bacteria belonging to the genera <i>Aeromonas, Acinetobacter, Vibrio, Pseudomonas, Escherichia</i> and <i>Yersinia</i>.</p><p><strong>Results: </strong>The results indicated that the most resistant bacteria were <i>Aeromonas, Pseudomonas</i> and <i>Acinetobacter</i>, while enteric bacteria demonstrated greater sensitivity. It is noteworthy that nitrofurantoin, a commonly used antibiotic for treating urinary tract infections, exhibited the highest level of resistance among the antibiotics tested by disc diffusion, followed by cephalosporins and penicillins.</p><p><strong>Conclusions: </strong>The MIC testing with DKGM and NF kits demonstrated high resistance to cephalosporins, sulfonamides, polymyxins and monobactams. Furthermore, two multidrug-resistant (MDR) isolates exhibited resistance to at least two antibiotic classes. These findings underscore the necessity for expanded antimicrobial resistance surveillance beyond clinical settings, extending into environmental samples, and contributing to ongoing research on resistance mechanisms.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"32 4","pages":"504-510"},"PeriodicalIF":1.2,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Depression, traumatic cognition, and death anxiety in pre-hospital and emergency staff depending on prior COVID-19 infection - a Turkish example.","authors":"Ali Doğan, Gülseren Keskin","doi":"10.26444/aaem/210348","DOIUrl":"https://doi.org/10.26444/aaem/210348","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Pre-hospital emergency health staff (PHEHS) and emergency service staff (ESS) who were directly involved in the fight against COVID-19, have been the most affected group among health service units. The aim of the study is to evaluate the traumatic cognition, depression and death anxiety according to having had the COVID-19 disease.</p><p><strong>Material and methods: </strong>A cross-sectional study was conducted between 15 December 2021-1 April 2022 in Gümüşhane, Turkey, with the participation of PHEHS and ESS (N=304. The Post-Traumatic Cognition Scale (PTCI), Beck Depression Scale (BDI) and Turkish Death Anxiety Inventory (TDAI) were used.</p><p><strong>Results: </strong>Based on the scoring ranges of the instruments used, the study found that participants exhibited moderate levels of depression (BDI scores between 17-29), high levels of death anxiety (TDAI scores approaching the upper limit of 80), and elevated trauma-related cognitions (PTCI scores within the higher range of 36-252, indicating increased negative cognitions related to the traumatic event). The mean scores of the PTCI and BDI were significantly higher among employees diagnosed with COVID-19 compared to those who were not (p < 0.05). Conversely, the mean scores of the TDAI were significantly higher among participants who had not been diagnosed with COVID-19 (p < 0.05). A gender-based analysis revealed that female participants scored significantly higher on the PTCI than male participants (t = -8.634, p < 0.05). Furthermore, a strong positive correlation was observed between BDI and PTCI scores (r = 0.822), indicating that increased depressive symptoms were associated with intensified trauma-related cognitions.</p><p><strong>Conclusions: </strong>Participants had moderate depression, moderate traumatic findings and moderate death anxiety; whereas participants diagnosed with COVID-19 had higher average of trauma and depression findings, lower death anxiety. It is important to take psycho-social measures for PHEHS and ESS providing health services, to take special precautions especially for women and employees diagnosed with COVID-19 who are more affected by the process, to supply and inspect equipment such as personal protective equipment.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"32 4","pages":"511-517"},"PeriodicalIF":1.2,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}