Forestry research最新文献

{"title":"Genetic selection for growth, wood quality and resin traits of potential Slash pine for multiple industrial uses.","authors":"Xianyin Ding, Yini Zhang, Jiaming Sun, Zifeng Tan, Qinyun Huang, Shu Diao, Yadi Wu, Qifu Luan, Jingmin Jiang","doi":"10.48130/forres-0024-0020","DOIUrl":"https://doi.org/10.48130/forres-0024-0020","url":null,"abstract":"<p><p>This study aims to understand the genetic basis of key industrial traits in Slash pine (<i>Pinus elliottii</i> Engelm. var. <i>elliottii</i>) to enhance improvement efficiency. Detailed analyses were conducted on inter-family differences, genetic parameters, correlations, and breeding values (BVs) for growth, wood properties, and resin traits of Slash pine planted in Changle Forest Farm of Hangzhou, leading to the identification of elite families. It indicates that growth traits are primarily influenced by environmental effects, while wood properties exhibit a significant impact of genetic effects. The variation in resin traits arises from both genetic and environmental effects. Notably, Beta-pinene exhibits the highest variability and genetic gains among the traits analyzed. The family heritability ranges for growth, wood properties, and resin traits are 0.543-0.794, 0.870-0.885, and 0.285-0.695, respectively. Significant positive correlations are evident between growth and resin traits, while a negative correlation is observed between growth and wood properties. Elite families identified through single-trait and multi-trait combined selection are 8-126 for growth traits, 2-325 and 0-373 for wood properties, and 8-131 for resin traits. The average genetic gains for these elite families are 7.44%, 7.17%, and 8.84%, respectively. These findings provide valuable insights for high-generation breeding of Slash pine and lay a genetic foundation for formulating effective breeding strategies for conifers.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e023"},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A birch ELONGATED HYPOCOTYL 5 gene enhances UV-B and drought tolerance.","authors":"Shangzhu Gao, Xiaohui Chen, Meihan Lin, Yibo Yin, Xiaoyi Li, Yaguang Zhan, Ying Xin, Fansuo Zeng","doi":"10.48130/forres-0024-0019","DOIUrl":"https://doi.org/10.48130/forres-0024-0019","url":null,"abstract":"<p><p>UV-B radiation and drought majorly restrict plant growth, particularly in summer. ELONGATED HYPOCOTYL 5 (HY5), a bZIP transcription factor (TF), has a beneficial impact on photomorphogenesis. However, the sequence of <i>HY5</i> from <i>Betula platyphylla</i> (<i>BpHY5</i>) has not been identified and the gene functions remain unclarified. We cloned the sequence of BpHY5, which was targeted to the nucleus. The hypocotyl phenotypes of heterologous expression in <i>Arabidopsis thaliana</i> and reverse mutation showed that <i>BpHY5</i> is homologous to <i>AtHY5</i>. The expression of <i>BpHY5</i> was increased in response to UV-B radiation, drought conditions, and the presence of abscisic acid (ABA). The overexpression of <i>BpHY5</i> resulted in increased tolerance to UV-B radiation and drought and decreased ABA sensitivity with higher germination and greening rate, more developmental root system, stronger reactive oxygen species scavenging ability, and lower damage degree. The lignin content under UV-B condition of <i>BpHY5</i>/Col was higher than that of Col. Furthermore, overexpressing <i>BpHY5</i> up-regulated the expression of genes related to tolerance (<i>NCED3/9</i>, <i>ABI5</i>, <i>DREB2A</i>, <i>RD20</i>, <i>ERF4</i>, <i>NDB2,</i> and <i>APX2</i>). In brief, the study suggests that <i>BpHY5</i> from birch serves as a beneficial modulator of plant responses to UV-B radiation and drought stress.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e022"},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.","authors":"Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi","doi":"10.48130/forres-0024-0017","DOIUrl":"https://doi.org/10.48130/forres-0024-0017","url":null,"abstract":"<p><p>Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) plays a crucial role in relative gene expression analysis, and accurate normalization relies on suitable reference genes (RGs). In this study, a pipeline for identifying candidate RGs from publicly available stem-related RNA-Seq data of different <i>Populus</i> species under various developmental and abiotic stress conditions is presented. DESeq2's median of ratios yielded the smallest coefficient of variance (CV) values in a total of 292 RNA-Seq samples and was therefore chosen as the method for sample normalization. A total of 541 stably expressed genes were retrieved based on the CV values with a cutoff of 0.3. Universal gene-specific primer pairs were designed based on the consensus sequences of the orthologous genes of each <i>Populus</i> RG candidate. The expression levels of 12 candidate RGs and six reported RGs in stems under different abiotic stress conditions or in different <i>Populus</i> species were assessed by RT-qPCR. The expression stability of selected genes was further evaluated using ΔCt, geNorm, NormFinder, and BestKeeper. All candidate RGs were stably expressed in different experiments and conditions in <i>Populus</i>. A test dataset containing 117 RNA-Seq samples was then used to confirm the expression stability, six candidate RGs and three reported RGs met the requirement of CV ≤ 0.3. In summary, this study was to propose a systematic and optimized protocol for the identification of constitutively and stably expressed genes based on RNA-Seq data, and Potri.001G349400 (<i>CNOT2</i>) was identified as the best candidate RG suitable for gene expression studies in poplar stems.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e021"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From haploid to reference: a new milestone in poplar genomics.","authors":"Shihui Niu, Quanzi Li","doi":"10.48130/forres-0024-0018","DOIUrl":"https://doi.org/10.48130/forres-0024-0018","url":null,"abstract":"","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e020"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nearly gapless, highly contiguous reference genome for a doubled haploid line of <i>Populus ussuriensis</i>, enabling advanced genomic studies.","authors":"Wenxuan Liu, Caixia Liu, Song Chen, Meng Wang, Xinyu Wang, Yue Yu, Ronald R Sederoff, Hairong Wei, Xiangling You, Guanzheng Qu, Su Chen","doi":"10.48130/forres-0024-0016","DOIUrl":"https://doi.org/10.48130/forres-0024-0016","url":null,"abstract":"<p><p><i>Populus</i> species, particularly <i>P. trichocarpa</i>, have long served as model trees for genomics research, owing to fully sequenced genomes. However, the high heterozygosity, and the presence of repetitive regions, including centromeres and ribosomal RNA gene clusters, have left 59 unresolved gaps, accounting for approximately 3.32% of the <i>P. trichocarpa</i> genome. In this study, the callus induction method was improved to derive a doubled haploid (DH) callus line from <i>P. ussuriensis</i> anthers. Leveraging long-read sequencing, we successfully assembled a nearly gap-free, telomere-to-telomere (T2T) <i>P. ussuriensis</i> genome spanning 412.13 Mb. This genome assembly contains only seven gaps and has a contig N50 length of 19.50 Mb. Annotation revealed 34,953 protein-coding genes in this genome, which is 465 more than that of <i>P. trichocarpa</i>. Notably, centromeric regions are characterized by higher-order repeats, we identified and annotated centromere regions in all DH genome chromosomes, a first for poplars. The derived DH genome exhibits high collinearity with <i>P. trichocarpa</i> and significantly fills gaps present in the latter's genome. This T2T <i>P. ussuriensis</i> reference genome will not only enhance our understanding of genome structure, and functions within the poplar genus but also provides valuable resources for poplar genomic and evolutionary studies.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e019"},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forest point cloud registration: a review.","authors":"Jincheng Liu, Yijun Guo, Juntao Yang, Ningning Zhu, Wenxia Dai, Qiang Yu","doi":"10.48130/forres-0024-0015","DOIUrl":"https://doi.org/10.48130/forres-0024-0015","url":null,"abstract":"<p><p>Point cloud registration is a necessary prerequisite for conducting precise, large-scale forest surveys and management. This paper focuses on providing a systematic overview and summary of the work on forest point cloud registration over the past 20 years. The developmental process of forest point cloud registration methods, spanning from the early reliance on manual markers to the subsequent evolution towards automatic registration based on feature matching, and then to the advanced technology based on deep learning were reviewed. Furthermore, the paper offered detailed discussions on the registration between different point cloud platforms: ground platforms, between ground platforms and aerial platforms, and between aerial platforms. Additionally, the paper delved into mainstream datasets and evaluation metrics in the domain of forest point cloud registration. Finally, the paper summarized the current state of research in this area, highlighted challenges, and provided future research outlooks. This review aims to provide researchers with a comprehensive understanding of forest point cloud registration, and to promote the advancement of point cloud technology, hopefully inspiring further applications in the field.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e018"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Walnut phosphatase 2A proteins interact with basic leucine zipper protein JrVIP1 to regulate osmotic stress response <i>via</i> calcium signaling.","authors":"Yi He, Shuwen Chen, Chenhao Li, Shen Yang, Zhongyu Sun, Siyu Hou, Zhenggang Xu, Guiyan Yang","doi":"10.48130/forres-0024-0012","DOIUrl":"https://doi.org/10.48130/forres-0024-0012","url":null,"abstract":"<p><p>Walnut is an important economic tree species that is susceptible to osmotic stress. Scientific cultivation management is an important way to improve the yield and quality of walnuts, which requires understanding the regulatory mechanisms in response to osmotic stress. Therefore, in this study, 15 protein phosphatase 2A (<i>PP2A</i>) genes were identified from the walnut transcriptome (named <i>JrPP2A01</i>~<i>15</i>) and their potential function responses to osmotic stress were elucidated. The open reading frame (ORF) of <i>JrPP2A01~15</i> ranges from 651 to 1,764 bp in length, the molecular weight of the encoded proteins are 24.15-65.61 kDa, and the theoretical isoelectric points are 4.80-8.37. These <i>JrPP2As</i> were unevenly distributed on 10 chromosomes and divided into five groups based on the composition of conserved domains, motifs, and exon/intron organizations. The five groups are <i>JrPP2AAs</i>, <i>JrPP2AB's,</i> <i>JrPP2AB''s</i>, <i>JrPP2AB55s</i>, and <i>JrPP2ACs</i>, including 1, 5, 2, 3, and 4 members, accordingly. The <i>cis</i>-elements in <i>JrPP2As</i>' promoters were involved in responses to hormone and abiotic stress. Most <i>JrPP2A</i> genes, excluding <i>JrPP2A01</i>, <i>JrPP2A02</i>, <i>JrPP2A05</i>, <i>JrPP2A06</i>, and <i>JrPP2A13</i>, could be induced significantly by PEG₆₀₀₀, NaCl, CaCl₂ and ABA. JrPP2A02, JrPP2A05, JrPP2A07, JrPP2A09, and JrPP2A14, could independently interact with a bZIP transcription factor JrVIP1. Moreover, overexpression of <i>JrPP2A07, JrPP2A09</i>, and <i>JrPP2A14</i> could significantly decrease ROS accumulation while increasing calcium (Ca) uptake exposed to PEG₆₀₀₀ and NaCl stresses, which was mediated by exogenous CaCl₂ and ABA. These results suggested that <i>JrPP2A</i> genes play potential key roles in walnut response to drought and salt-inducing osmotic stress involving Ca- and ABA-dependent signaling pathways.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e016"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus uptake, transport, and signaling in woody and model plants.","authors":"Xingyan Fang, Deming Yang, Lichuan Deng, Yaxin Zhang, Zhiyong Lin, Jingjing Zhou, Zhichang Chen, Xiangqing Ma, Meina Guo, Zhaohua Lu, Liuyin Ma","doi":"10.48130/forres-0024-0014","DOIUrl":"https://doi.org/10.48130/forres-0024-0014","url":null,"abstract":"<p><p>Phosphorus (P), a critical macronutrient for plant growth and reproduction, is primarily acquired and translocated in the form of inorganic phosphate (Pi) by roots. Pi deficiency is widespread in many natural ecosystems, including forest plantations, due to its slow movement and easy fixation in soils. Plants have evolved complex and delicate regulation mechanisms on molecular and physiological levels to cope with Pi deficiency. Over the past two decades, extensive research has been performed to decipher the underlying molecular mechanisms that regulate the Pi starvation responses (PSR) in plants. This review highlights the prospects of Pi uptake, transport, and signaling in woody plants based on the backbone of model and crop plants. In addition, this review also highlights the interactions between phosphorus and other mineral nutrients such as Nitrogen (N) and Iron (Fe). Finally, this review discusses the challenges and potential future directions of Pi research in woody plants, including characterizing the woody-specific regulatory mechanisms of Pi signaling and evaluating the regulatory roles of Pi on woody-specific traits such as wood formation and ultimately generating high Phosphorus Use Efficiency (PUE) woody plants.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e017"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype-resolved genome assembly of poplar line NL895 provides a valuable tree genomic resource.","authors":"Jie Luo, Yan Wang, Zihui Li, Ziwei Wang, Xu Cao, Nian Wang","doi":"10.48130/forres-0024-0013","DOIUrl":"https://doi.org/10.48130/forres-0024-0013","url":null,"abstract":"<p><p>Poplar line NL895 can potentially become a model plant for poplar study as it is a widely cultivated elite line. However, the lack of genome resources hindered the use of NL895 as the major plant material in poplar. In this study, we provided a high-quality genome assembly for poplar line NL895 with PacBio single molecule real-time (SMRT) sequencing and High-throughput chromosome conformation capture (Hi-C) technology. The raw assembly of NL895 for the diploid genome included 606 contigs with a total size of ~815 Mb, and the monoploid genome included 246 contigs with a total size of ~412 Mb. The haplotype-resolved chromosomes in the diploid genomes were also generated. All the monoploid, diploid, and haplotype-resolved genomes showed more than 97% completeness and they can largely improve the mapping efficiency in RNA-Seq analysis. By comprehensively comparing the two haplotype genomes we found the heterozygosity of NL895 is much higher than other poplar lines. We also found that NL895 harbors more genomic variants and more gene diversity. The haplotype-specific genes showed higher variable gene expression patterns. These characters would be attributed to the high heterosis of poplar line NL895. The allele-specific expression (ASE) was also investigated and lots of alleles showed biased expressions in different tissues or environmental conditions. Taken together, the genome sequence for NL895 is a valuable tree genomic resource and it would greatly facilitate studies in poplar.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e015"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics reveal the involvement of reactive oxygen species production and sequestration during stigma development and pollination in <i>Fraxinus mandshurica</i>.","authors":"Shuqi Wang, Shun Yang, Bello Hassan Jakada, Hongtao Qin, Yaguang Zhan, Xingguo Lan","doi":"10.48130/forres-0024-0011","DOIUrl":"https://doi.org/10.48130/forres-0024-0011","url":null,"abstract":"<p><p>Stigma development and successful pollination are essential for the continuous existence of flowering plants. However, the specific mechanisms regulating these important processes are not well understood. In this study, we investigated the development of the stigma in <i>Fraxinus mandshurica</i>, dividing it into three stages: S1, S2, and S3. Transcriptome data were used to analyze the gene expression patterns across these developmental stages, and 6,402 genes were observed to exhibit variable expression levels. Our analysis revealed a significant enrichment of pathways related to reactive oxygen species (ROS) and flavonoids, as indicated by the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the differentially expressed genes. Further examination by cluster analysis and quantitative polymerase chain reaction revealed that 58 genes were associated with ROS synthesis and seven genes were linked to flavonoid synthesis during the S2 and S3 stages. ROS accumulated during stigma development, which decreased rapidly upon pollen germination and pollen tube elongation, as confirmed by H₂DCFDA staining. Moreover, ROS levels in mature stigmas were reduced by treatment with ROS scavengers, such as copper (II) chloride, sodium salicylate, and diphenyleneiodonium, an inhibitor of NADPH oxidases, which enhanced pollen adhesion and germination. These findings suggest that the balance between ROS production and sequestration plays a critical role in regulating stigma development and pollen germination in <i>Fraxinus mandshurica</i>.</p>","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e014"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}