Forestry research最新文献_第4页

IF 5

Forestry research Pub Date : 2025-11-19 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0028

Zha-Long Ye, Xiang-Yi Li, Man-Li Nong, Xiao-Mei Sun, Wanfeng Li

{"title":"The age-related expression patterns of Larix kaempferi AP2 subfamily genes and functional dissection of LkTOE1-2 in seed formation and germination.","authors":"Zha-Long Ye, Xiang-Yi Li, Man-Li Nong, Xiao-Mei Sun, Wanfeng Li","doi":"10.48130/forres-0025-0028","DOIUrl":"https://doi.org/10.48130/forres-0025-0028","url":null,"abstract":"Conifers pose challenges for breeding programs due to their extended vegetative phases. Despite the critical role of APETALA2 (AP2) in regulating phase transitions, the AP2/ERF superfamily remains largely unexplored in gymnosperms. Here, the first genome-wide analysis of the AP2/ERF superfamily in a conifer, Larix kaempferi (Japanese larch) is presented, and 374 members were identified. Among all eight paralogs, four euAP2 lineage genes, TARGET OF EATs (TOEs), exhibit age-decreased expression patterns. Functional characterization of LkTOE1-2 demonstrates its involvement in somatic embryogenesis and seed germination. Importantly, the RUBY reporter system confirmed an enhanced promoter activity in somatic embryo maturation. Over-expression of LkTOE1-2 in Arabidopsis accelerates seed germination by 23.77%, bolting by 6.93%, and flowering by 5.92%. This study provides not only an expanded genomic resource for gymnosperms but also a candidate gene (LkTOE1-2) to shorten the juvenile phase of larch via molecular breeding.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e027"},"PeriodicalIF":5.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12648160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic vulnerability assessment reveals the potential benefits of adaptive introgression by mitigating the maladaptive risk of admixed populations. 基因组脆弱性评估揭示了适应性基因渗入的潜在益处，可以减轻混合种群的不适应风险。

IF 5

Forestry research Pub Date : 2025-11-19 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0026

Wen-Hao Li, Han-Yang Lin, Chen Chen, Chen-Feng Lin, Xing-Xing Shen, Yun-Peng Zhao

{"title":"Genomic vulnerability assessment reveals the potential benefits of adaptive introgression by mitigating the maladaptive risk of admixed populations.","authors":"Wen-Hao Li, Han-Yang Lin, Chen Chen, Chen-Feng Lin, Xing-Xing Shen, Yun-Peng Zhao","doi":"10.48130/forres-0025-0026","DOIUrl":"https://doi.org/10.48130/forres-0025-0026","url":null,"abstract":"As climate change accelerates, plant species largely rely on genetic variation to adapt and survive when they fail to track their ecological niches through range shifts. Predicted genomic vulnerability is able to identify populations lacking the necessary genetic variation for climate change adaptation. However, the role of introgression in genomic vulnerability remains poorly explored. Here, we used the dove tree (Davidia involucrata), a relict species native to southwestern China, to test whether introgression may reduce genomic vulnerability. By integrating population genomics and environmental data collected from 196 individuals of 18 populations, we identified 747 strictly climate-associated loci across the distribution range of D. involucrata, 138 of which were recovered from the genetically admixed populations. We estimated the genomic vulnerability for three genetic lineages and two admixed groups using the gradient forest approach, and found that eastern populations are likely to be at higher risk. The eastern admixed populations exhibited a significant reduction, with introgression from the southern lineage. Cumulative importance analysis showed moderate importance for introgressive loci along environmental gradients. This indicates that the introduction of novel alleles through introgression provides only a partial and insufficient counterbalance to the maladaptation observed in D. involucrata under climate change. Our study highlights the role of intraspecific introgression in response to climate change and emphasizes the importance of genomic vulnerability studies in informing conservation practices for relict and endangered species.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e026"},"PeriodicalIF":5.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12648016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the RNA m5C methyltransferase genes in Populus alba × Populus glandulosa and the role of PagTRM4B in wood formation. 白杨×叶杨RNA m5C甲基转移酶基因的鉴定及PagTRM4B在木材形成中的作用

IF 5

Forestry research Pub Date : 2025-11-07 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0025

Jiahuan Chen, Guoning Qi, Minyi Zhong, Zhenghao Geng, Jingran Chen, Jie Zhao, Mingjia Chen, Xiaojing Yan

{"title":"Identification of the RNA m5C methyltransferase genes in Populus alba × Populus glandulosa and the role of PagTRM4B in wood formation.","authors":"Jiahuan Chen, Guoning Qi, Minyi Zhong, Zhenghao Geng, Jingran Chen, Jie Zhao, Mingjia Chen, Xiaojing Yan","doi":"10.48130/forres-0025-0025","DOIUrl":"https://doi.org/10.48130/forres-0025-0025","url":null,"abstract":"N5-methylcytosine (m5C) RNA methylation plays an essential role in gene regulation, yet its functions in woody plant development remain poorly understood. This study systematically identified 12 putative tRNA-specific methyltransferase 4 (TRM4) homologs (PagTRM4s) within the P. alba × P. glandulosa genome. Phylogenetic and synteny analyses revealed high evolutionary conservation across Arabidopsis and rice. Structural and domain analyses suggested functional conservation among allelic pairs. Expression profiling showed that PagTRM4B-a maintained stable expression in xylem at different developmental stages. PagTRM4B-a overexpression in poplar resulted in decreased plant height and stem diameter, modified wood composition, and increased RNA m5C levels. Histological analysis showed that overexpression enhanced xylem cell expansion and secondary xylem formation without affecting cambial activity. Moreover, the expression levels of secondary cell wall (SCW) biosynthesis genes were significantly down-regulated in PagTRM4B-a-OE transgenic plants. This study offers new insights into the epigenetic control of secondary growth in trees and highlights PagTRM4B-a as a potential target for influencing wood formation in woody plants.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e025"},"PeriodicalIF":5.0,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12648020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth strategies and phenotypic plasticity of improved Chinese fir families across soil types. 改良杉木科不同土壤类型的生长策略和表型可塑性。

IF 5

Forestry research Pub Date : 2025-10-30 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0022

Zhen Zhang, Wenyue Wang, Huimin Niu, Haobo Zhao, Jingyong Ji, Guiping He, Zhichun Zhou

{"title":"Growth strategies and phenotypic plasticity of improved Chinese fir families across soil types.","authors":"Zhen Zhang, Wenyue Wang, Huimin Niu, Haobo Zhao, Jingyong Ji, Guiping He, Zhichun Zhou","doi":"10.48130/forres-0025-0022","DOIUrl":"https://doi.org/10.48130/forres-0025-0022","url":null,"abstract":"To enhance phenotypic plasticity, it is vital to maximize the genetic growth potential of trees and understand their adaptive responses to environmental conditions. Tree species adapt to dynamic environmental conditions by leveraging the interactions among the environment, genotype, and genotype-by-environment. A total of 25 improved varieties of Chinese fir were transplanted and developed through multi-generational breeding into four types of artificial forest soils. Through a quantitative analysis of genotypic, soil environmental conditions, and genotype-by-environment interaction effects on variations in growth, biomass, and root functional traits, the key drivers of phenotypic plasticity were identified. The results indicate that soil environmental conditions and genotype-by-environment interactions are the primary factors influencing trait variation, explaining 55.89% to 93.94% of the observed variation, while the family effect is relatively minor. Notably, pronounced phenotypic plasticity drives divergent selection in both aboveground and belowground growth strategies. Critical traits influencing root dry weight include root average diameter, total root volume, and root-to-shoot ratio. Although root dry weight does not directly affect plant height, it has a substantial impact on aboveground dry weight. These findings highlight that the changes in the aboveground and belowground growth strategies of Chinese fir during the seedling stage are closely linked to the plasticity of root functional traits. For multi-generational genetically improved varieties, this study examined how the integration of genetic effects, soil environmental conditions, and genotype-by-environment interactions in the selection of aboveground growth and root functional traits influences the mechanisms driving biomass accumulation. The results provide actionable insights for selecting soil-specific families in subtropical plantations.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e024"},"PeriodicalIF":5.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12647935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coniferous-broadleaf mixed plantations reshape phosphorus-solubilizing bacterial communities and enhance soil phosphorus bioavailability in subtropical forests. 针叶阔叶混交林重塑了亚热带森林溶磷细菌群落，提高了土壤磷的生物有效性。

IF 5

Forestry research Pub Date : 2025-10-29 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0023

Yuting Zhang, Qiyan Liu, Kai Ding, Qinglin Sun, Meng Lu, Yifan Zhou, Qi Yang, Zaikang Tong, Junhong Zhang

{"title":"Coniferous-broadleaf mixed plantations reshape phosphorus-solubilizing bacterial communities and enhance soil phosphorus bioavailability in subtropical forests.","authors":"Yuting Zhang, Qiyan Liu, Kai Ding, Qinglin Sun, Meng Lu, Yifan Zhou, Qi Yang, Zaikang Tong, Junhong Zhang","doi":"10.48130/forres-0025-0023","DOIUrl":"https://doi.org/10.48130/forres-0025-0023","url":null,"abstract":"Mixed-species plantations are proven to enhance phosphorus (P) availability in subtropical forest ecosystems. However, the effect of coniferous-broadleaf mixed plantations on soil P cycling dynamics remains poorly understood. Through a long-term field experiment, the study investigated how mixed plantations influence soil P fractions, phoD and pqqC genes, and associated bacterial communities in bulk and rhizosphere soils. Results showed that compared to monocultures, the introduction of broad-leaved trees significantly increased labile P pools, particularly in the rhizosphere. Amplicon-based community profiling of phoD/pqqC genes demonstrated distinct compositional shifts in P-solubilizing bacterial communities across forest types and soil compartments. The pqqC-harboring bacterial communities were more closely related to the P fractions. More importantly, plant properties were important in explaining bulk soil labile P responses, while in rhizosphere soil, labile P was more strongly associated with soil properties, positively affecting labile P. These findings elucidate the complex interplay between tree diversity, microbial functional traits, and soil P transformations. This study underscores the critical role of mixed plantations in promoting microbial-mediated P mobilization and provides valuable insights for designing sustainable forest management strategies to optimize P utilization in subtropical ecosystems.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e022"},"PeriodicalIF":5.0,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12648022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing forest structure for sustainability: a review of structure-based management effects on stand quality. 优化森林结构促进可持续性：结构管理对林分质量的影响

IF 5

Forestry research Pub Date : 2025-10-29 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0024

Qiming Liao, Quan Qiu, Jie Gao, Qiang Liu, Qin Su, Yue Yang, Peilin Xie, Yutian Xin, Xiaolong Zhao, Pan Wan

{"title":"Optimizing forest structure for sustainability: a review of structure-based management effects on stand quality.","authors":"Qiming Liao, Quan Qiu, Jie Gao, Qiang Liu, Qin Su, Yue Yang, Peilin Xie, Yutian Xin, Xiaolong Zhao, Pan Wan","doi":"10.48130/forres-0025-0024","DOIUrl":"https://doi.org/10.48130/forres-0025-0024","url":null,"abstract":"Structure-based forest management (SBFM) has emerged as an innovative silvicultural approach that optimizes the spatial arrangement of trees to emulate natural forest structures and promote sustainability. Despite increasing applications of SBFM, a comprehensive synthesis of its impacts on stand quality and the underlying mechanisms remains lacking. This review synthesizes 126 peer-reviewed studies (2007-2025) to evaluate the multidimensional effects of SBFM on forest stand growth, structure, soil properties, and stability. Evidence indicates that SBFM enhances tree growth by reducing competitive pressure, improves diameter distribution and species mingling, thereby approximating natural stand patterns, and enriches soil carbon and nutrient pools through increased litter input and microbial activity. These structural adjustments collectively foster a positive feedback loop that integrates aboveground productivity, belowground processes, and overall ecosystem resilience. Future research should prioritize cross-regional ecological monitoring, mechanistic experiments that link structural optimization to biodiversity and carbon sequestration, the integration of artificial intelligence (AI) and remote sensing for precision management, and improvements in forest stand quality evaluation systems. Overall, SBFM markedly improves stand quality and constitutes a promising strategy for sustainable forest management that enhances ecological resilience.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e023"},"PeriodicalIF":5.0,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12648026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PtABI3 represses the age biomarker gene PtDAL1 during male cone development in conifer. PtABI3在针叶树雄性球果发育过程中抑制年龄标记基因PtDAL1。

IF 5

Forestry research Pub Date : 2025-09-29 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0021

Yi-Tong Song, Feng-Yi Li, Xi Chen, Huan-Huan Zhao, Dong-Yue Wang, Jing-Jing Li, Xiao-Han Zhang, Jun-He Yang, Fang-Xu Han, Pei-Yi Wang, Quan Zuo, Qian-Ya Zhu, Hui Zhang, Biao Zhou, Shi-Hui Niu

{"title":"PtABI3 represses the age biomarker gene PtDAL1 during male cone development in conifer.","authors":"Yi-Tong Song, Feng-Yi Li, Xi Chen, Huan-Huan Zhao, Dong-Yue Wang, Jing-Jing Li, Xiao-Han Zhang, Jun-He Yang, Fang-Xu Han, Pei-Yi Wang, Quan Zuo, Qian-Ya Zhu, Hui Zhang, Biao Zhou, Shi-Hui Niu","doi":"10.48130/forres-0025-0021","DOIUrl":"10.48130/forres-0025-0021","url":null,"abstract":"The age biomarker gene PtDAL1 in conifers undergoes expression resetting during pollen maturation, but its regulatory mechanisms remain unclear. This study identifies PtABI3, a B3 transcription factor (TF) in Pinus tabuliformis, as a key repressor of PtDAL1. PtABI3 exhibits strict spatiotemporal complementarity with PtDAL1. Phylogenetic and structural studies revealed PtABI3 as a gymnosperm homolog of angiosperm ABI3, retaining conserved B3 domains critical for DNA binding and transcriptional repression. Ectopic expression of PtABI3 in Arabidopsis recapitulated the classic ABI3 late-flowering phenotype. Mechanistically, PtABI3 directly binds the RY motif (CATGCA) within the PtDAL1 promoter via its B3 domain, as demonstrated by dual-luciferase assays, yeast one-hybrid assays, and electrophoretic mobility shift assays (EMSA). These results revealed that PtABI3 is an evolutionary conserved transcriptional repressor that silences PtDAL1 during pollen maturation, providing the first evidence of B3 TFs mediating age timer gene resetting in conifers. This work bridges gymnosperm and angiosperm regulatory paradigms, highlighting ancient mechanisms for gene expression resetting in plants.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e021"},"PeriodicalIF":5.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12569429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145411308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative transcriptome and metabolome evaluation of melanin biosynthesis in Phyllostachys nigra during low-temperature growth. 黑毛竹低温生长过程中黑色素生物合成的综合转录组和代谢组评估。

IF 5

Forestry research Pub Date : 2025-09-23 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0020

Haojie Wang, Yadan Cao, Shawn D Mansfield, Pengwei Zhang, Xinchun Lin, Dan Hou

{"title":"Integrative transcriptome and metabolome evaluation of melanin biosynthesis in Phyllostachys nigra during low-temperature growth.","authors":"Haojie Wang, Yadan Cao, Shawn D Mansfield, Pengwei Zhang, Xinchun Lin, Dan Hou","doi":"10.48130/forres-0025-0020","DOIUrl":"10.48130/forres-0025-0020","url":null,"abstract":"Plant melanin is an organic molecule commonly used in medicine, food, and chemical industries. However, the molecular underpinnings of plant melanin's biosynthesis and its regulation are still unclear. Phyllostachys nigra is well known for its ornamental value because of its black culms. The black pigments enriched in the epidermis and cortex of P. nigra were identified to be melanin by analyses of its physical and chemical properties. Moreover, the biosynthesis of melanin was examined using comprehensive transcriptomic and metabolomic analyses in P. nigra when grown at low temperatures. Nontargeted metabolite profiling revealed that some indoles, including serotonin, 3-indoleacetic acid, and 1H-indole-3-acetamide, were significantly enriched in P. nigra when grown at low temperatures. Parallel transcriptomic analysis showed that a set of structural genes involved in serotonin biosynthesis was significantly upregulated by low temperatures. By integrating the transcriptome data and weighted gene co-expression network analysis, the essential transcription factors that putatively regulate the biosynthesis of serotonins were revealed. Among those, PnWRKY19-3 was functionally tested and shown to increase the serotonin content in transgenic rice by upregulating OsT5H under low temperature conditions. These findings suggest that PnWRKY19-3 may play a positive role in promoting melanin formation in the culms of P. nigra. According to the two functional genomic platforms, it appears that low temperature stimulates melanin formation in P. nigra by inducing the biosynthesis of indoles. Our research provides new insights into melanin biosynthesis in bamboo, which may be vital to other plant species.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e020"},"PeriodicalIF":5.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12464485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase of Tree Species Mingling enhances the resistance of Pinus armandii forests to Dendroctonus armandi infestation. 树种混交的增加增强了胡松林对胡松林侵染的抵抗力。

IF 5

Forestry research Pub Date : 2025-09-16 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0019

Zhaolong Li, Xiaozi Zhou, Ziyan Zhang, Jie Fan, Yuanyong Dian

{"title":"Increase of Tree Species Mingling enhances the resistance of Pinus armandii forests to Dendroctonus armandi infestation.","authors":"Zhaolong Li, Xiaozi Zhou, Ziyan Zhang, Jie Fan, Yuanyong Dian","doi":"10.48130/forres-0025-0019","DOIUrl":"10.48130/forres-0025-0019","url":null,"abstract":"Pinus armandii Franch., a key native conifer in China, faces severe Dendroctonus armandi infestation, threatening forest ecosystems. Reducing infestation probability and enhancing resistance are essential for transforming Pinus armandii forests. This study investigates the correlation between stand structure, topographic factors, and the probability of Pinus armandii infestation by Dendroctonus armandi. Based on these correlations, it selects suitable mixed-species combinations of native tree species with low infestation probabilities that are adapted to regional characteristics. A random survey was conducted in 58 plots (6,021 trees) in Shennongjia. Logistic regression and analysis of variance (ANOVA) revealed: (1) Infestation rate increased with elevation, peaking at 84.38% above 2,000 m; between 1,500-2,100 m, probability rose 4.3% per 100 m elevation gain; (2) Steeper slopes (> 25°) reduced infestation (46.03%), with risk decreasing 1.9% per 1° slope increase (0°-40°); (3) Larger DBH (> 30 cm) trees had higher infestation (82.93%), increasing 4.5% per 1 cm DBH; (4) Higher species mingling (four neighboring non-Pinus trees) lowered infestation to 63.39%, reducing risk by 54.3% per mingling unit; (5) Healthy Pinus armandii were frequently neighbored by Litsea pungens, Carpinus cordata, Phellodendron chinense, and Betula platyphylla. Prioritizing slopes > 25° and elevations < 2,000 m for afforestation, mixed with Litsea pungens, Carpinus cordata, or Betula platyphylla, can mitigate infestation. These findings provide actionable strategies to enhance Pinus armandii forest resilience against Dendroctonus armandi threats.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e019"},"PeriodicalIF":5.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PagARF3.1 promotes adventitious root formation by repressing IPT-mediated cytokinin biosynthesis. PagARF3.1通过抑制ipt介导的细胞分裂素生物合成促进不定根的形成。

IF 5

Forestry research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.48130/forres-0025-0018

Ying-Li Liu, Xue-Qin Song, Hui He, Meng-Xuan Hu, Meng-Zhu Lu, Shu-Tang Zhao

{"title":"PagARF3.1 promotes adventitious root formation by repressing IPT-mediated cytokinin biosynthesis.","authors":"Ying-Li Liu, Xue-Qin Song, Hui He, Meng-Xuan Hu, Meng-Zhu Lu, Shu-Tang Zhao","doi":"10.48130/forres-0025-0018","DOIUrl":"10.48130/forres-0025-0018","url":null,"abstract":"Adventitious root formation is a crucial developmental process in the clonal propagation of economically significant horticultural and woody species. This process is antagonistically regulated by auxin and cytokinin, yet the underlying molecular regulatory mechanisms remain poorly understood. In this study, we investigated the role of PagARF3.1, a homolog of Arabidopsis auxin response factor 3, in hybrid poplar (Populus alba × Populus glandulosa clone cv. '84K'), focusing on its involvement in adventitious root formation. GUS staining analysis revealed that PagARF3.1 was expressed in adventitious root tips, pericycle cells, early root primordia, and outgrowing roots. Knockdown of PagARF3.1 delayed adventitious root formation and reduced root biomass in transgenic plants, whereas overexpression of PagARF3.1 promoted earlier rooting and increased the number of adventitious roots. Real-time quantitative polymerase chain reaction analysis indicated that the expression levels of PagIPT5a and PagIPT5b were significantly elevated in PagARF3.1 RNAi lines and reduced in overexpression lines. Yeast one-hybrid assays and ChIP-PCR analysis demonstrated that PagARF3.1 directly binds to the promoter regions of PagIPT5a and PagIPT5b, thereby regulating their expression. These findings collectively demonstrate that PagARF3.1 acts as a positive regulator of adventitious root formation by repressing IPT-mediated cytokinin biosynthesis.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"5 ","pages":"e018"},"PeriodicalIF":5.0,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0