Forestry research最新文献_第4页

Haplotype-resolved genome assembly of poplar line NL895 provides a valuable tree genomic resource. 杨树品系 NL895 的单倍型解析基因组组装提供了宝贵的树木基因组资源。

Forestry research Pub Date : 2024-04-23 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0013

Jie Luo, Yan Wang, Zihui Li, Ziwei Wang, Xu Cao, Nian Wang

{"title":"Haplotype-resolved genome assembly of poplar line NL895 provides a valuable tree genomic resource.","authors":"Jie Luo, Yan Wang, Zihui Li, Ziwei Wang, Xu Cao, Nian Wang","doi":"10.48130/forres-0024-0013","DOIUrl":"https://doi.org/10.48130/forres-0024-0013","url":null,"abstract":"Poplar line NL895 can potentially become a model plant for poplar study as it is a widely cultivated elite line. However, the lack of genome resources hindered the use of NL895 as the major plant material in poplar. In this study, we provided a high-quality genome assembly for poplar line NL895 with PacBio single molecule real-time (SMRT) sequencing and High-throughput chromosome conformation capture (Hi-C) technology. The raw assembly of NL895 for the diploid genome included 606 contigs with a total size of ~815 Mb, and the monoploid genome included 246 contigs with a total size of ~412 Mb. The haplotype-resolved chromosomes in the diploid genomes were also generated. All the monoploid, diploid, and haplotype-resolved genomes showed more than 97% completeness and they can largely improve the mapping efficiency in RNA-Seq analysis. By comprehensively comparing the two haplotype genomes we found the heterozygosity of NL895 is much higher than other poplar lines. We also found that NL895 harbors more genomic variants and more gene diversity. The haplotype-specific genes showed higher variable gene expression patterns. These characters would be attributed to the high heterosis of poplar line NL895. The allele-specific expression (ASE) was also investigated and lots of alleles showed biased expressions in different tissues or environmental conditions. Taken together, the genome sequence for NL895 is a valuable tree genomic resource and it would greatly facilitate studies in poplar.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e015"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomics reveal the involvement of reactive oxygen species production and sequestration during stigma development and pollination in Fraxinus mandshurica. 转录组学揭示了Fraxinus mandshurica柱头发育和授粉过程中活性氧的产生和螯合。

Forestry research Pub Date : 2024-04-23 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0011

Shuqi Wang, Shun Yang, Bello Hassan Jakada, Hongtao Qin, Yaguang Zhan, Xingguo Lan

{"title":"Transcriptomics reveal the involvement of reactive oxygen species production and sequestration during stigma development and pollination in Fraxinus mandshurica.","authors":"Shuqi Wang, Shun Yang, Bello Hassan Jakada, Hongtao Qin, Yaguang Zhan, Xingguo Lan","doi":"10.48130/forres-0024-0011","DOIUrl":"https://doi.org/10.48130/forres-0024-0011","url":null,"abstract":"Stigma development and successful pollination are essential for the continuous existence of flowering plants. However, the specific mechanisms regulating these important processes are not well understood. In this study, we investigated the development of the stigma in Fraxinus mandshurica, dividing it into three stages: S1, S2, and S3. Transcriptome data were used to analyze the gene expression patterns across these developmental stages, and 6,402 genes were observed to exhibit variable expression levels. Our analysis revealed a significant enrichment of pathways related to reactive oxygen species (ROS) and flavonoids, as indicated by the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the differentially expressed genes. Further examination by cluster analysis and quantitative polymerase chain reaction revealed that 58 genes were associated with ROS synthesis and seven genes were linked to flavonoid synthesis during the S2 and S3 stages. ROS accumulated during stigma development, which decreased rapidly upon pollen germination and pollen tube elongation, as confirmed by H2DCFDA staining. Moreover, ROS levels in mature stigmas were reduced by treatment with ROS scavengers, such as copper (II) chloride, sodium salicylate, and diphenyleneiodonium, an inhibitor of NADPH oxidases, which enhanced pollen adhesion and germination. These findings suggest that the balance between ROS production and sequestration plays a critical role in regulating stigma development and pollen germination in Fraxinus mandshurica.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e014"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining spatial units for modeling regional nonnative invasive plant species spread in the southern US forestlands: using the state of Alabama as an example. 确定美国南部林地区域非外来入侵植物物种传播模型的空间单位：以阿拉巴马州为例。

Forestry research Pub Date : 2024-04-11 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0010

Sunil Nepal, Martin A Spetich, Zhaofei Fan

{"title":"Determining spatial units for modeling regional nonnative invasive plant species spread in the southern US forestlands: using the state of Alabama as an example.","authors":"Sunil Nepal, Martin A Spetich, Zhaofei Fan","doi":"10.48130/forres-0024-0010","DOIUrl":"https://doi.org/10.48130/forres-0024-0010","url":null,"abstract":"Nonnative invasive plant species (NNIPS) cause significant damage to the native forest ecosystems in the southern United States forestlands, such as habitat degradation, ecological instability, and biodiversity loss. Taking the state of Alabama as an example, we used more than 5,000 permanent United States Department of Agriculture-Forest Service's Forest Inventory and Analysis (FIA) plots measured between 2001 and 2019 over three measurement cycles to test the suitable modeling unit for quantifying invasion patterns and associated factors for regional NNIPS monitoring and management. NNIPS heavily infest Alabama's forestlands, and forestlands plagued with at least one NNIPS have increased over time: 41.1%, 50.8%, and 54.8% during the past three measurements. Lonicera japonica (Thunb.) was the most abundant NNIPS in Alabama, with at least 26% of its forested lands infested. The FIA data were aggregated with multiple spatial units: five levels of hydrological units, three levels of ecological units, and a county level. Invasion indices were calculated for all spatial units based on NNIPS' presence/absence and average cover in each plot. The best modeling unit was identified based on Moran's test, with the county-level modeling unit providing the best Moran's I value over all measurement periods. Influencing factors of invasion were evaluated based on spatial lag models. Our models show that the invasion index decreased with increases in public forest areas in a county. In contrast, the human population density of neighboring counties positively influenced the invasion index.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e013"},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance. 人类 TTP 基因在杨树中的异位表达可促进木质部分化并赋予植物耐旱性。

Forestry research Pub Date : 2024-04-09 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0008

Yamei Zhuang, Yang Chen, Qiao Wang, Yan Chen, Liping Yan, Shengjun Li, Gongke Zhou, Guohua Chai

引用次数: 0

Selective sweep and GWAS provide insights into adaptive variation of Populus cathayana leaves. 选择性扫描和基因组测序为了解杨树叶片的适应性变异提供了线索。

Forestry research Pub Date : 2024-04-09 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0009

Xinglu Zhou, Xiaodong Xiang, Demei Cao, Lei Zhang, Jianjun Hu

{"title":"Selective sweep and GWAS provide insights into adaptive variation of Populus cathayana leaves.","authors":"Xinglu Zhou, Xiaodong Xiang, Demei Cao, Lei Zhang, Jianjun Hu","doi":"10.48130/forres-0024-0009","DOIUrl":"https://doi.org/10.48130/forres-0024-0009","url":null,"abstract":"Leaf morphology plays a crucial role in predicting the productivity and environmental adaptability of forest trees, making it essential to understand the genetic mechanisms behind leaf variation. In natural populations of Populus cathayana, leaf morphology exhibits rich intraspecific variation due to long-term selection. However, there have been no studies that systematically reveal the genetic mechanisms of leaf variation in P. cathayana. To fill this gap and enhance our understanding of leaf variation in P. cathayana, we collected nine leaf traits from the P. cathayana natural population, consisting of 416 accessions, and conducted the preliminary classification of leaf types with four categories. Subsequently, we conducted an analysis of selective sweep and genome-wide association studies (GWAS) to uncover the genetic basis of leaf traits variation. Most of the leaf traits displayed significant correlations, with broad-sense trait heritability ranging from 0.38 to 0.74. In total, three selective sweep methods ultimately identified 278 positively selected candidate regions and 493 genes associated with leaf size. Single-trait and multi-trait GWAS methods detected 13 and 59 genes, respectively. By integrating the results of selective sweep and GWAS, we further identified a total of nine overlapping genes. These genes may play a role in the leaf development process and are closely associated with leaf size. In particular, the gene CBSCBSPB3 (Pca07G009100) located on chromosome 7, was associated with the response to light stimulation. This study will deepen our understanding of the genetic mechanism of leaf adaptive variation in P. cathayana and provide valuable gene resources.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e012"},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic transformation in conifers: current status and future prospects. 针叶树的遗传转化：现状与前景。

Forestry research Pub Date : 2024-03-21 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0007

Huanhuan Zhao, Jinfeng Zhang, Jian Zhao, Shihui Niu

{"title":"Genetic transformation in conifers: current status and future prospects.","authors":"Huanhuan Zhao, Jinfeng Zhang, Jian Zhao, Shihui Niu","doi":"10.48130/forres-0024-0007","DOIUrl":"https://doi.org/10.48130/forres-0024-0007","url":null,"abstract":"Genetic transformation has been a cornerstone in plant molecular biology research and molecular design breeding, facilitating innovative approaches for the genetic improvement of trees with long breeding cycles. Despite the profound ecological and economic significance of conifers in global forestry, the application of genetic transformation in this group has been fraught with challenges. Nevertheless, genetic transformation has achieved notable advances in certain conifer species, while these advances are confined to specific genotypes, they offer valuable insights for technological breakthroughs in other species. This review offers an in-depth examination of the progress achieved in the genetic transformation of conifers. This discussion encompasses various factors, including expression vector construction, gene-delivery methods, and regeneration systems. Additionally, the hurdles encountered in the pursuit of a universal model for conifer transformation are discussed, along with the proposal of potential strategies for future developments. This comprehensive overview seeks to stimulate further research and innovation in this crucial field of forest biotechnology.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e010"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of nitrogen water interaction on leaf functional traits of dominant species in warm temperate forest. 氮水相互作用对暖温带森林优势物种叶片功能特征的影响

Forestry research Pub Date : 2024-03-15 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0006

Wen Li, Mingyang Liu, Mengke Li, Ruomin Sun, Tenglong Zhou, Yaqi He, Jianing Mao, Chang Liu, Lei Ma, Shenglei Fu

{"title":"Influence of nitrogen water interaction on leaf functional traits of dominant species in warm temperate forest.","authors":"Wen Li, Mingyang Liu, Mengke Li, Ruomin Sun, Tenglong Zhou, Yaqi He, Jianing Mao, Chang Liu, Lei Ma, Shenglei Fu","doi":"10.48130/forres-0024-0006","DOIUrl":"https://doi.org/10.48130/forres-0024-0006","url":null,"abstract":"Plant functional traits are indicative of plant responses to environmental changes, influencing ecosystem functions. Leaves, as a key focus in studying plant functional traits, present an area where the impact of nitrogen deposition and altered rainfall patterns on functional diversity remains ambiguous. To elucidate plant response mechanisms to environmental factors, we employed a canopy-based platform to add nitrogen, water, and their combination. We assessed the functional traits and community-weighted mean of the leaves of three dominant trees and three dominant shrubs. The results showed that nitrogen addition to the canopy significantly increased the leaf dry matter content of the Celtis sinensis Pers, but markedly decreased the specific leaf area of the Liquidambar formosana Hance. The nitrogen-water interaction did not notably affect the specific leaf area and equivalent water thickness of leaves. Canopy addition of nitrogen, water, and their combined interaction substantially lowered leaf nitrogen content and markedly increased leaf C/N. The structural equation model demonstrated a significant negative correlation between leaf dry matter content, equivalent water thickness, and leaf nitrogen content, as well as between equivalent water thickness and leaf phosphorus content. Our results provide evidence for the adaptation of plants to the environment and different strategies for resource and energy utilization.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e009"},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeography of Pterocarya hupehensis reveals the evolutionary patterns of a Cenozoic relict tree around the Sichuan Basin. 紫檀的系统地理学揭示了四川盆地一带新生代孑遗树种的演化模式。

Forestry research Pub Date : 2024-03-12 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0005

Zi-Jia Lu, Tian-Rui Wang, Si-Si Zheng, Hong-Hu Meng, Jian-Guo Cao, Yi-Gang Song, Gregor Kozlowski

{"title":"Phylogeography of Pterocarya hupehensis reveals the evolutionary patterns of a Cenozoic relict tree around the Sichuan Basin.","authors":"Zi-Jia Lu, Tian-Rui Wang, Si-Si Zheng, Hong-Hu Meng, Jian-Guo Cao, Yi-Gang Song, Gregor Kozlowski","doi":"10.48130/forres-0024-0005","DOIUrl":"https://doi.org/10.48130/forres-0024-0005","url":null,"abstract":"Environmental factors such as mountain tectonic movements and monsoons can enhance genetic differentiation by hindering inter- and intra-specific gene flow. However, the phylogeographic breaks detected within species may differ depending on the different molecular markers used, and biological traits may be a major confounding factor. Pterocarya hupehensis is a vulnerable relict species distributed throughout the Sichuan Basin. Here, we investigated the phylogeographic patterns and evolutionary history of P. hupehensis using chloroplast DNA and restriction site-associated DNA sequencing data from 18 populations around the Sichuan Basin. The 24 chloroplast haplotypes separated into western and eastern lineages at approximately 16.7 Mya, largely coincident with a strengthening of the East Asian monsoon system during the early to middle Miocene. Both cpDNA and nuclear DNA datasets consistently identified distinct western and eastern lineages whose phylogeographic break conformed to the boundary of the Sino-Himalayan and Sino-Japanese forest sub-kingdoms. However, in contrast to the nuclear gene data, the cpDNA data revealed further divergence of the eastern lineage into northern and southern groups along the Yangtze River, a result that likely reflects differences in the extent of pollen vs seed dispersal. During the temperature decline in the penultimate (Riss) glacial period of the Pleistocene epoch, P. hupehensis experienced a genetic bottleneck event, and ecological niche modeling suggests that a subsequent population expansion occurred during the last interglacial period. Our findings not only establish a basis for conservation of this species, but also serve as a case study for the effects of geography and climate change on the evolutionary history of wind-pollinated relict plants.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e008"},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in Liriodendron hybrids. 建立糖皮质激素诱导系统，调节 Liriodendron 杂交种的体细胞胚胎发生。

Forestry research Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0003

Xinying Chen, Ye Liu, Lu Lu, Siqin Liu, Yuhao Weng, Jisen Shi, Zhaodong Hao, Jinhui Chen

{"title":"Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in Liriodendron hybrids.","authors":"Xinying Chen, Ye Liu, Lu Lu, Siqin Liu, Yuhao Weng, Jisen Shi, Zhaodong Hao, Jinhui Chen","doi":"10.48130/forres-0024-0003","DOIUrl":"https://doi.org/10.48130/forres-0024-0003","url":null,"abstract":"The precise expression of transcription factors (TFs) is crucial for plant growth and development, especially during somatic embryogenesis. However, conventional overexpression approaches, commonly used for functional genetics, can lead to deleterious effects. Therefore, it is imperative to ensure that TFs are expressed in a controlled and timely manner when aiming to enhance the efficiency of somatic embryogenesis. In this study, a dexamethasone/glucocorticoid receptor (DEX/GR) inducible expression system was employed to modulate the protein expression levels of target TFs within the nucleus during somatic embryogenesis in Liriodendron hybrids. We selected the WUSCHEL (WUS) gene, a well-established functional TF known for its vital role in somatic embryogenesis, as a model to assess the effectiveness of this system. Through DEX treatment, we induced the translocation of LhWUS-GR/LhWUS-GFP-GR fusion proteins from the cytoplasm to the nucleus, consequently leading to WUS-driven somatic embryogenesis. As the DEX concentration increased, there was a corresponding increase in the migration of the LhWUS-GFP-GR fusion protein into the nucleus. Additionally, we observed a higher proliferation ratio of callus expressing LhWUS-GR when exposed to varying DEX concentrations. Notably, the efficiency of somatic embryogenesis exhibited significant improvement under optimal DEX concentration. In conclusion, our study successfully utilizes the DEX/GR inducible system in Liriodendron hybrids, offering a valuable tool for the precise control and utilization of TFs at the desired levels. This innovative approach holds promise for advancing our understanding of TF function and enhancing plant development through the regulated manipulation of TF expression.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e006"},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis reveals genes associated with the bitter-sweet trait of apricot kernels. 转录组分析揭示了与杏核苦甜性状相关的基因。

Forestry research Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0004

Yu Zhang, Wenquan Bao, Ta-Na Wuyun, Mengzhen Huang, Chen Chen, Dun Ao, Rong Yang, Haiguang Huang, Lin Wang

{"title":"Transcriptome analysis reveals genes associated with the bitter-sweet trait of apricot kernels.","authors":"Yu Zhang, Wenquan Bao, Ta-Na Wuyun, Mengzhen Huang, Chen Chen, Dun Ao, Rong Yang, Haiguang Huang, Lin Wang","doi":"10.48130/forres-0024-0004","DOIUrl":"https://doi.org/10.48130/forres-0024-0004","url":null,"abstract":"Prunasin and amygdalin are important factors that influence the kernel taste of apricots, however, the regulatory mechanisms underlying this are unclear. In this study, we analyzed the phenotype and transcriptome of kernels during development in Prunus sibirica (bitter kernels) and Prunus armeniaca × Prunus sibirica (kernel consumption apricot, sweet kernels). Prunasin and amygdalin content was significantly higher in bitter kernels compared with that in sweet kernels. Prunasin content exhibited a decreasing trend in both bitter and sweet kernels. The fastest decline was observed in bitter and sweet kernels during S3-S4 (82.21%) and S2-S3 (59.65%), respectively. The amygdalin content in the bitter kernels exhibited the fastest increase between 45-60 d after flowering, and reached a peak at 6.22% on 60 d after flowering. In contrast, the peak in sweet kernels occurred at 60 d after flowering, with a much lower content of 0.18%. Transcriptome analysis revealed 6,942 differentially expressed genes (DEGs), with a subset of 38 DEGs specifically enriched in the cyanoamino acid metabolic pathway. Among these, the ten candidate genes, including CYP79, CYP71, UGT1, AH, and PH, were identified as crucial in regulating prunasin and amygdalin metabolism. Furthermore, a weighted gene co-expression network analysis (WGCNA) unveiled two modules that exhibited significant correlation with prunasin and amygdalin content. Five DEGs were located at the center of the co-expression network, and were identified as hub genes, with four positively regulating prunasin content and one negatively regulating amygdalin content. Our results provide novel insights into the molecular-level regulation of the apricot kernel taste.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e007"},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0