Forestry research最新文献_第4页

Determining spatial units for modeling regional nonnative invasive plant species spread in the southern US forestlands: using the state of Alabama as an example. 确定美国南部林地区域非外来入侵植物物种传播模型的空间单位：以阿拉巴马州为例。

Forestry research Pub Date : 2024-04-11 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0010

Sunil Nepal, Martin A Spetich, Zhaofei Fan

{"title":"Determining spatial units for modeling regional nonnative invasive plant species spread in the southern US forestlands: using the state of Alabama as an example.","authors":"Sunil Nepal, Martin A Spetich, Zhaofei Fan","doi":"10.48130/forres-0024-0010","DOIUrl":"https://doi.org/10.48130/forres-0024-0010","url":null,"abstract":"Nonnative invasive plant species (NNIPS) cause significant damage to the native forest ecosystems in the southern United States forestlands, such as habitat degradation, ecological instability, and biodiversity loss. Taking the state of Alabama as an example, we used more than 5,000 permanent United States Department of Agriculture-Forest Service's Forest Inventory and Analysis (FIA) plots measured between 2001 and 2019 over three measurement cycles to test the suitable modeling unit for quantifying invasion patterns and associated factors for regional NNIPS monitoring and management. NNIPS heavily infest Alabama's forestlands, and forestlands plagued with at least one NNIPS have increased over time: 41.1%, 50.8%, and 54.8% during the past three measurements. Lonicera japonica (Thunb.) was the most abundant NNIPS in Alabama, with at least 26% of its forested lands infested. The FIA data were aggregated with multiple spatial units: five levels of hydrological units, three levels of ecological units, and a county level. Invasion indices were calculated for all spatial units based on NNIPS' presence/absence and average cover in each plot. The best modeling unit was identified based on Moran's test, with the county-level modeling unit providing the best Moran's I value over all measurement periods. Influencing factors of invasion were evaluated based on spatial lag models. Our models show that the invasion index decreased with increases in public forest areas in a county. In contrast, the human population density of neighboring counties positively influenced the invasion index.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e013"},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance. 人类 TTP 基因在杨树中的异位表达可促进木质部分化并赋予植物耐旱性。

Forestry research Pub Date : 2024-04-09 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0008

Yamei Zhuang, Yang Chen, Qiao Wang, Yan Chen, Liping Yan, Shengjun Li, Gongke Zhou, Guohua Chai

引用次数: 0

Selective sweep and GWAS provide insights into adaptive variation of Populus cathayana leaves. 选择性扫描和基因组测序为了解杨树叶片的适应性变异提供了线索。

Forestry research Pub Date : 2024-04-09 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0009

Xinglu Zhou, Xiaodong Xiang, Demei Cao, Lei Zhang, Jianjun Hu

{"title":"Selective sweep and GWAS provide insights into adaptive variation of Populus cathayana leaves.","authors":"Xinglu Zhou, Xiaodong Xiang, Demei Cao, Lei Zhang, Jianjun Hu","doi":"10.48130/forres-0024-0009","DOIUrl":"https://doi.org/10.48130/forres-0024-0009","url":null,"abstract":"Leaf morphology plays a crucial role in predicting the productivity and environmental adaptability of forest trees, making it essential to understand the genetic mechanisms behind leaf variation. In natural populations of Populus cathayana, leaf morphology exhibits rich intraspecific variation due to long-term selection. However, there have been no studies that systematically reveal the genetic mechanisms of leaf variation in P. cathayana. To fill this gap and enhance our understanding of leaf variation in P. cathayana, we collected nine leaf traits from the P. cathayana natural population, consisting of 416 accessions, and conducted the preliminary classification of leaf types with four categories. Subsequently, we conducted an analysis of selective sweep and genome-wide association studies (GWAS) to uncover the genetic basis of leaf traits variation. Most of the leaf traits displayed significant correlations, with broad-sense trait heritability ranging from 0.38 to 0.74. In total, three selective sweep methods ultimately identified 278 positively selected candidate regions and 493 genes associated with leaf size. Single-trait and multi-trait GWAS methods detected 13 and 59 genes, respectively. By integrating the results of selective sweep and GWAS, we further identified a total of nine overlapping genes. These genes may play a role in the leaf development process and are closely associated with leaf size. In particular, the gene CBSCBSPB3 (Pca07G009100) located on chromosome 7, was associated with the response to light stimulation. This study will deepen our understanding of the genetic mechanism of leaf adaptive variation in P. cathayana and provide valuable gene resources.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e012"},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic transformation in conifers: current status and future prospects. 针叶树的遗传转化：现状与前景。

Forestry research Pub Date : 2024-03-21 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0007

Huanhuan Zhao, Jinfeng Zhang, Jian Zhao, Shihui Niu

{"title":"Genetic transformation in conifers: current status and future prospects.","authors":"Huanhuan Zhao, Jinfeng Zhang, Jian Zhao, Shihui Niu","doi":"10.48130/forres-0024-0007","DOIUrl":"https://doi.org/10.48130/forres-0024-0007","url":null,"abstract":"Genetic transformation has been a cornerstone in plant molecular biology research and molecular design breeding, facilitating innovative approaches for the genetic improvement of trees with long breeding cycles. Despite the profound ecological and economic significance of conifers in global forestry, the application of genetic transformation in this group has been fraught with challenges. Nevertheless, genetic transformation has achieved notable advances in certain conifer species, while these advances are confined to specific genotypes, they offer valuable insights for technological breakthroughs in other species. This review offers an in-depth examination of the progress achieved in the genetic transformation of conifers. This discussion encompasses various factors, including expression vector construction, gene-delivery methods, and regeneration systems. Additionally, the hurdles encountered in the pursuit of a universal model for conifer transformation are discussed, along with the proposal of potential strategies for future developments. This comprehensive overview seeks to stimulate further research and innovation in this crucial field of forest biotechnology.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e010"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of nitrogen water interaction on leaf functional traits of dominant species in warm temperate forest. 氮水相互作用对暖温带森林优势物种叶片功能特征的影响

Forestry research Pub Date : 2024-03-15 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0006

Wen Li, Mingyang Liu, Mengke Li, Ruomin Sun, Tenglong Zhou, Yaqi He, Jianing Mao, Chang Liu, Lei Ma, Shenglei Fu

{"title":"Influence of nitrogen water interaction on leaf functional traits of dominant species in warm temperate forest.","authors":"Wen Li, Mingyang Liu, Mengke Li, Ruomin Sun, Tenglong Zhou, Yaqi He, Jianing Mao, Chang Liu, Lei Ma, Shenglei Fu","doi":"10.48130/forres-0024-0006","DOIUrl":"https://doi.org/10.48130/forres-0024-0006","url":null,"abstract":"Plant functional traits are indicative of plant responses to environmental changes, influencing ecosystem functions. Leaves, as a key focus in studying plant functional traits, present an area where the impact of nitrogen deposition and altered rainfall patterns on functional diversity remains ambiguous. To elucidate plant response mechanisms to environmental factors, we employed a canopy-based platform to add nitrogen, water, and their combination. We assessed the functional traits and community-weighted mean of the leaves of three dominant trees and three dominant shrubs. The results showed that nitrogen addition to the canopy significantly increased the leaf dry matter content of the Celtis sinensis Pers, but markedly decreased the specific leaf area of the Liquidambar formosana Hance. The nitrogen-water interaction did not notably affect the specific leaf area and equivalent water thickness of leaves. Canopy addition of nitrogen, water, and their combined interaction substantially lowered leaf nitrogen content and markedly increased leaf C/N. The structural equation model demonstrated a significant negative correlation between leaf dry matter content, equivalent water thickness, and leaf nitrogen content, as well as between equivalent water thickness and leaf phosphorus content. Our results provide evidence for the adaptation of plants to the environment and different strategies for resource and energy utilization.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e009"},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeography of Pterocarya hupehensis reveals the evolutionary patterns of a Cenozoic relict tree around the Sichuan Basin. 紫檀的系统地理学揭示了四川盆地一带新生代孑遗树种的演化模式。

Forestry research Pub Date : 2024-03-12 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0005

Zi-Jia Lu, Tian-Rui Wang, Si-Si Zheng, Hong-Hu Meng, Jian-Guo Cao, Yi-Gang Song, Gregor Kozlowski

{"title":"Phylogeography of Pterocarya hupehensis reveals the evolutionary patterns of a Cenozoic relict tree around the Sichuan Basin.","authors":"Zi-Jia Lu, Tian-Rui Wang, Si-Si Zheng, Hong-Hu Meng, Jian-Guo Cao, Yi-Gang Song, Gregor Kozlowski","doi":"10.48130/forres-0024-0005","DOIUrl":"https://doi.org/10.48130/forres-0024-0005","url":null,"abstract":"Environmental factors such as mountain tectonic movements and monsoons can enhance genetic differentiation by hindering inter- and intra-specific gene flow. However, the phylogeographic breaks detected within species may differ depending on the different molecular markers used, and biological traits may be a major confounding factor. Pterocarya hupehensis is a vulnerable relict species distributed throughout the Sichuan Basin. Here, we investigated the phylogeographic patterns and evolutionary history of P. hupehensis using chloroplast DNA and restriction site-associated DNA sequencing data from 18 populations around the Sichuan Basin. The 24 chloroplast haplotypes separated into western and eastern lineages at approximately 16.7 Mya, largely coincident with a strengthening of the East Asian monsoon system during the early to middle Miocene. Both cpDNA and nuclear DNA datasets consistently identified distinct western and eastern lineages whose phylogeographic break conformed to the boundary of the Sino-Himalayan and Sino-Japanese forest sub-kingdoms. However, in contrast to the nuclear gene data, the cpDNA data revealed further divergence of the eastern lineage into northern and southern groups along the Yangtze River, a result that likely reflects differences in the extent of pollen vs seed dispersal. During the temperature decline in the penultimate (Riss) glacial period of the Pleistocene epoch, P. hupehensis experienced a genetic bottleneck event, and ecological niche modeling suggests that a subsequent population expansion occurred during the last interglacial period. Our findings not only establish a basis for conservation of this species, but also serve as a case study for the effects of geography and climate change on the evolutionary history of wind-pollinated relict plants.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e008"},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in Liriodendron hybrids. 建立糖皮质激素诱导系统，调节 Liriodendron 杂交种的体细胞胚胎发生。

Forestry research Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0003

Xinying Chen, Ye Liu, Lu Lu, Siqin Liu, Yuhao Weng, Jisen Shi, Zhaodong Hao, Jinhui Chen

{"title":"Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in Liriodendron hybrids.","authors":"Xinying Chen, Ye Liu, Lu Lu, Siqin Liu, Yuhao Weng, Jisen Shi, Zhaodong Hao, Jinhui Chen","doi":"10.48130/forres-0024-0003","DOIUrl":"https://doi.org/10.48130/forres-0024-0003","url":null,"abstract":"The precise expression of transcription factors (TFs) is crucial for plant growth and development, especially during somatic embryogenesis. However, conventional overexpression approaches, commonly used for functional genetics, can lead to deleterious effects. Therefore, it is imperative to ensure that TFs are expressed in a controlled and timely manner when aiming to enhance the efficiency of somatic embryogenesis. In this study, a dexamethasone/glucocorticoid receptor (DEX/GR) inducible expression system was employed to modulate the protein expression levels of target TFs within the nucleus during somatic embryogenesis in Liriodendron hybrids. We selected the WUSCHEL (WUS) gene, a well-established functional TF known for its vital role in somatic embryogenesis, as a model to assess the effectiveness of this system. Through DEX treatment, we induced the translocation of LhWUS-GR/LhWUS-GFP-GR fusion proteins from the cytoplasm to the nucleus, consequently leading to WUS-driven somatic embryogenesis. As the DEX concentration increased, there was a corresponding increase in the migration of the LhWUS-GFP-GR fusion protein into the nucleus. Additionally, we observed a higher proliferation ratio of callus expressing LhWUS-GR when exposed to varying DEX concentrations. Notably, the efficiency of somatic embryogenesis exhibited significant improvement under optimal DEX concentration. In conclusion, our study successfully utilizes the DEX/GR inducible system in Liriodendron hybrids, offering a valuable tool for the precise control and utilization of TFs at the desired levels. This innovative approach holds promise for advancing our understanding of TF function and enhancing plant development through the regulated manipulation of TF expression.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e006"},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis reveals genes associated with the bitter-sweet trait of apricot kernels. 转录组分析揭示了与杏核苦甜性状相关的基因。

Forestry research Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.48130/forres-0024-0004

Yu Zhang, Wenquan Bao, Ta-Na Wuyun, Mengzhen Huang, Chen Chen, Dun Ao, Rong Yang, Haiguang Huang, Lin Wang

{"title":"Transcriptome analysis reveals genes associated with the bitter-sweet trait of apricot kernels.","authors":"Yu Zhang, Wenquan Bao, Ta-Na Wuyun, Mengzhen Huang, Chen Chen, Dun Ao, Rong Yang, Haiguang Huang, Lin Wang","doi":"10.48130/forres-0024-0004","DOIUrl":"https://doi.org/10.48130/forres-0024-0004","url":null,"abstract":"Prunasin and amygdalin are important factors that influence the kernel taste of apricots, however, the regulatory mechanisms underlying this are unclear. In this study, we analyzed the phenotype and transcriptome of kernels during development in Prunus sibirica (bitter kernels) and Prunus armeniaca × Prunus sibirica (kernel consumption apricot, sweet kernels). Prunasin and amygdalin content was significantly higher in bitter kernels compared with that in sweet kernels. Prunasin content exhibited a decreasing trend in both bitter and sweet kernels. The fastest decline was observed in bitter and sweet kernels during S3-S4 (82.21%) and S2-S3 (59.65%), respectively. The amygdalin content in the bitter kernels exhibited the fastest increase between 45-60 d after flowering, and reached a peak at 6.22% on 60 d after flowering. In contrast, the peak in sweet kernels occurred at 60 d after flowering, with a much lower content of 0.18%. Transcriptome analysis revealed 6,942 differentially expressed genes (DEGs), with a subset of 38 DEGs specifically enriched in the cyanoamino acid metabolic pathway. Among these, the ten candidate genes, including CYP79, CYP71, UGT1, AH, and PH, were identified as crucial in regulating prunasin and amygdalin metabolism. Furthermore, a weighted gene co-expression network analysis (WGCNA) unveiled two modules that exhibited significant correlation with prunasin and amygdalin content. Five DEGs were located at the center of the co-expression network, and were identified as hub genes, with four positively regulating prunasin content and one negatively regulating amygdalin content. Our results provide novel insights into the molecular-level regulation of the apricot kernel taste.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"4 ","pages":"e007"},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of 5mC DNA demethylation via 5-aza-2'-deoxycytidine in regulating gene expression during early somatic embryo development in white spruce (Picea glauca). 通过 5-aza-2'-deoxycytidine 使 5mC DNA 去甲基化参与调节白云杉早期体细胞胚胎发育过程中的基因表达。

Forestry research Pub Date : 2023-12-26 eCollection Date: 2023-01-01 DOI: 10.48130/fr-0023-0030

Ying Gao, Xiaoyi Chen, Chengbi Liu, Huanhuan Zhao, Fengbin Dai, Jian Zhao, Jinfeng Zhang, Lisheng Kong

{"title":"Involvement of 5mC DNA demethylation via 5-aza-2'-deoxycytidine in regulating gene expression during early somatic embryo development in white spruce (Picea glauca).","authors":"Ying Gao, Xiaoyi Chen, Chengbi Liu, Huanhuan Zhao, Fengbin Dai, Jian Zhao, Jinfeng Zhang, Lisheng Kong","doi":"10.48130/fr-0023-0030","DOIUrl":"https://doi.org/10.48130/fr-0023-0030","url":null,"abstract":"DNA methylation plays a crucial role in the development of somatic embryos (SEs) through the regulation of gene expression. To examine the impact of DNA methylation on gene expression during early SE development in Picea glauca, the demethylation reagent 5-aza-dC (5-aza-2'-deoxycytidine) was employed to modify DNA methylation regions and levels during the pre-maturation stage of somatic embryogenesis. The application of 2.0 µM 5-aza-dC did not induce toxicity to SEs in early development. Following treatment, the global DNA methylation level decreased significantly on the 7th day of pre-maturation and the 1st week of maturation. Methylated DNA immunoprecipitation (MeDIP) sequencing revealed that differentially methylated regions, as analyzed through Gene Ontology (GO), were related to plant development and reproduction and that they were hypomethylated on the 3rd day but hypermethylated on the 7th day in 5-aza-dC-treated embryogenic tissues. These findings indicate that 5-aza-dC treatment positively impacts early SE development, which was inhibited following 7 d of treatment. The expression of MSH7, JMJ14, and CalS10 was associated with DNA methylation, epigenetic regulation, and somatic embryogenesis. Further analysis of methylated regions revealed that the expression profiles of MSH7, JMJ14, and CalS10 were correlated with altered DNA methylation, suggesting DNA methylation at 5 mC may play a role in controlling the expression of these genes and regulating the early development of SEs in P. glauca. This study offers new insights into the regulation of somatic embryogenesis in conifers.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"3 ","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide discovery of CBL genes in Nitraria tangutorum Bobr. and functional analysis of NtCBL1-1 under drought and salt stress. Nitraria tangutorum Bobr.中 CBL 基因的全基因组发现以及 NtCBL1-1 在干旱和盐胁迫下的功能分析。

Forestry research Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.48130/FR-2023-0028

Liming Zhu, Jingxiang Wu, Mengjuan Li, Hao Fang, Jingbo Zhang, Yuchang Chen, Jinhui Chen, Tielong Cheng

{"title":"Genome-wide discovery of CBL genes in Nitraria tangutorum Bobr. and functional analysis of NtCBL1-1 under drought and salt stress.","authors":"Liming Zhu, Jingxiang Wu, Mengjuan Li, Hao Fang, Jingbo Zhang, Yuchang Chen, Jinhui Chen, Tielong Cheng","doi":"10.48130/FR-2023-0028","DOIUrl":"https://doi.org/10.48130/FR-2023-0028","url":null,"abstract":"Calcineurin B-like (CBL) proteins are a class of important Ca2+ receptors that play key roles in plant stress response. CBLs have been shown to participate in responses to abiotic stresses such as drought, salt, and cold in many plant species, including Arabidopsis and rice. However, little is known about their potential functions in the desert halophyte Nitraria tangutorum. Here, we have identified 11 CBL genes distributed across six chromosomes of N. tangutorum and categorized them into four groups through phylogenetic analysis. Synteny analysis showed that they have strong collinear relationships and have undergone purifying selection during their evolution. NtCBL promoter regions contain a variety of cis-acting elements related to hormone and environmental stress responses. Real-time quantitative PCR showed that the expression of NtCBLs differed significantly among various tissues and was upregulated by salt and drought stress. We chose NtCBL1-1 for an in-depth functional characterization and observed that transgenic Arabidopsis plants expressing NtCBL1-1 exhibited increased tolerance to both drought and salt stress. Compared to wild-type Arabidopsis, transgenic lines showed higher germination rates, slower chlorophyll degradation, more soluble proteins, and reduced accumulation of the oxidative stress marker malondialdehyde. These findings indicate that NtCBL1-1 plays a significant role in responding to drought and salt stress, laying the foundation for further investigations into the functional mechanisms of NtCBL genes in N. tangutorum.","PeriodicalId":520285,"journal":{"name":"Forestry research","volume":"3 ","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0