Npj viruses最新文献_第4页

Pathogenesis of aquatic bird bornavirus 1 in turkeys of different age. 水禽博纳病毒1型在不同年龄火鸡中的发病机制。

Npj viruses Pub Date : 2025-02-24 DOI: 10.1038/s44298-025-00095-z

Lisa Gordon, Alexander Leacy, Phuc H Pham, Jaime Tuling, Sunoh Che, Antonius El-Khoury, Jeff L Caswell, Brandon N Lillie, Leonardo Susta

{"title":"Pathogenesis of aquatic bird bornavirus 1 in turkeys of different age.","authors":"Lisa Gordon, Alexander Leacy, Phuc H Pham, Jaime Tuling, Sunoh Che, Antonius El-Khoury, Jeff L Caswell, Brandon N Lillie, Leonardo Susta","doi":"10.1038/s44298-025-00095-z","DOIUrl":"https://doi.org/10.1038/s44298-025-00095-z","url":null,"abstract":"Aquatic bird bornavirus 1 (ABBV1), an orthobornavirus in the family Bornaviridae, displays a broad host range among avian species, including poultry. The pathogenesis of orthobornaviruses, at least in mammals and psittacines, appears to be mediated by the host immune response against the infected nervous tissue, with younger animals showing a milder disease due to immune tolerance. Here, we tested the ability of ABBV1 to infect domestic turkeys (Meleagris gallopavo), with a focus on evaluating the impact of age at infection. Cohorts of 6-week-old (old) and day-old (young) male turkeys were divided into virus-inoculated and control groups, and kept for up to 12 weeks. Results showed that turkeys of both ages were susceptible to ABBV1 infection by intramuscular administration, following a centripetal and limited centrifugal spread, although infection appeared delayed in old compared to young birds. Notably, only young turkeys developed clinical signs and more frequent inflammation of the central nervous system, indicating that infection at a very early age is unlikely to induce tolerance to ABBV1 infection.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the hidden diversity of Chlorella heliozoae-infecting giant viruses. 揭示感染小球藻的巨型病毒的隐藏多样性。

Npj viruses Pub Date : 2025-02-22 DOI: 10.1038/s44298-025-00088-y

Lethícia R Henriques, Bruna B F Botelho, Roger M Carlson, João Victor R P Carvalho, Ellen G Oliveira, Irina V Agarkova, James L Van Etten, David D Dunigan, Rodrigo A L Rodrigues

{"title":"Revealing the hidden diversity of Chlorella heliozoae-infecting giant viruses.","authors":"Lethícia R Henriques, Bruna B F Botelho, Roger M Carlson, João Victor R P Carvalho, Ellen G Oliveira, Irina V Agarkova, James L Van Etten, David D Dunigan, Rodrigo A L Rodrigues","doi":"10.1038/s44298-025-00088-y","DOIUrl":"https://doi.org/10.1038/s44298-025-00088-y","url":null,"abstract":"A new level of viral complexity has emerged from the isolation of green algae-infecting chloroviruses from diverse aquatic environments around the world over the past few decades. This study focuses on describing and comparing the genomic features of gammachloroviruses, previously referred to as SAG-viruses. We present 24 novel isolates capable of forming plaques on lawns of Chlorella heliozoae SAG 3.83, including the first giant virus isolated from Greenland. Together with 13 previous isolates, these new viruses form a robust dataset that we used to investigate the genomic landscape and to test whether environmental conditions influence the species diversity of gammachloroviruses. Genome sizes range from 283 kbp to 385 kbp, with one new isolate having the smallest genome found in the genus Chlorovirus. Based on phylogenomics and global genome identity analysis, we defined 10 species of \"Gammachlorovirus\", half of which are represented by a single isolate. We observed a high level of genome synteny, and the tRNA islets maintain a distinct interspecific pattern, although some notable variations are evident. Our analysis reveals an open pan-genome composed of 681 COGs, more than 30% of which consist of uncharacterized genes, highlighting significant innovative genetic potential for these viruses. Our results suggest that the subgenus \"Gammachlorovirus\" exhibits the greatest genetic diversity among chloroviruses, with variability that is independent of geographic location. Overall, these findings underscore the considerable diversity within these ten newly defined species and the importance of isolating and characterizing chloroviruses from new locations worldwide to enhance our understanding of the ecology and evolution of this group of giant algal viruses.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recruitment of apolipoprotein E facilitates Herpes simplex virus 1 attachment and release. 载脂蛋白E的募集促进单纯疱疹病毒1的附着和释放。

Npj viruses Pub Date : 2025-02-22 DOI: 10.1038/s44298-025-00099-9

Lifeng Liu, Fouzia Bano, Dario Valter Conca, Konrad Thorsteinsson, Sanduni Wasana Jayaweera, Damien Avinens, Hudson Pace, Hugo Lövheim, Anders Olofsson, Marta Bally

{"title":"Recruitment of apolipoprotein E facilitates Herpes simplex virus 1 attachment and release.","authors":"Lifeng Liu, Fouzia Bano, Dario Valter Conca, Konrad Thorsteinsson, Sanduni Wasana Jayaweera, Damien Avinens, Hudson Pace, Hugo Lövheim, Anders Olofsson, Marta Bally","doi":"10.1038/s44298-025-00099-9","DOIUrl":"https://doi.org/10.1038/s44298-025-00099-9","url":null,"abstract":"Human apolipoprotein E (ApoE) has been shown to play important roles during primary infection and pathogenesis of several viruses. Furthermore, epidemiological studies suggest that interactions between ApoE 4 and herpes simplex virus type-1 (HSV1) could associate with higher risk of Alzheimer's disease. Nevertheless, little is known about the ApoE-HSV1 interactions at molecular levels. Here, we investigate the effects of ApoE on HSV1 infection in vitro. Our results show that ApoE promotes HSV1 growth, which is attributed to the incorporation of ApoE into HSV1 particles. Using both biological and biophysical approaches, we conclude that ApoE-coated HSV1 demonstrates a more efficient attachment to and faster release from the cell surface. Mechanistic studies reveal that ApoE modifies HSV1 interactions with heparan sulfate, thereby modulating interactions between HSV1 and the cell surface. Overall, our results provide new insights into the roles of ApoE during HSV1 infections which may inspire future studies on Alzheimer's disease etiology.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Mpox in Central Africa: complex epidemiology requires a constellation approach. 作者更正：中非的麻疹：复杂的流行病学需要采取联合方法。

Npj viruses Pub Date : 2025-02-21 DOI: 10.1038/s44298-025-00097-x

Megan Halbrook, Jean Claude Makangara-Cigolo, Sydney Merritt, Nicole A Hoff, Laurens Liesenborghs, Lisa E Hensley, Koen Vercauteren, Placide Mbala-Kingebeni, Anne W Rimoin, Jason Kindrachuk

引用次数: 0

20 years of research on giant viruses. 20年来对巨型病毒的研究。

Npj viruses Pub Date : 2025-02-11 DOI: 10.1038/s44298-025-00093-1

Tressy Bosmon, Chantal Abergel, Jean-Michel Claverie

引用次数: 0

Cytochrome b5 occurrence in giant and other viruses belonging to the phylum Nucleocytoviricota. 细胞色素b5存在于巨病毒和其他核细胞病毒门的病毒中。

Npj viruses Pub Date : 2025-02-11 DOI: 10.1038/s44298-025-00091-3

David C Lamb, Jared V Goldstone, Djamal Brahim Belhaouari, Julien Andréani, Ayesha Farooqi, Michael J Allen, Steven L Kelly, Bernard La Scola, John J Stegeman

{"title":"Cytochrome b5 occurrence in giant and other viruses belonging to the phylum Nucleocytoviricota.","authors":"David C Lamb, Jared V Goldstone, Djamal Brahim Belhaouari, Julien Andréani, Ayesha Farooqi, Michael J Allen, Steven L Kelly, Bernard La Scola, John J Stegeman","doi":"10.1038/s44298-025-00091-3","DOIUrl":"https://doi.org/10.1038/s44298-025-00091-3","url":null,"abstract":"Cytochrome b5 is an electron transport protein found in eukaryotes and bacteria, and plays roles in energy production, lipid biosynthesis and cytochrome P450 biochemistry. Here we report that genes for cytochrome b5 occur broadly among viruses in the class Megaviricetes isolated from the deep ocean, freshwater and terrestrial sources, and human patients. Transcriptional analysis showed that Mimivirus bradfordmassiliense cytochrome b5 is expressed in the host and has characteristic spectral properties. Viral cytochrome b5s have either a unique N-terminal transmembrane anchor or are predicted to be soluble proteins. Virus cytochrome b5 proteins share 45-95% sequence identity with one another but no more than 25% identity with that in Acanthamoeba castellanii, a host for many giant viruses. Thus, the origin of cytochrome b5 genes in giant viruses remains unknown. Our findings raise questions regarding the evolution and diversity of cytochrome b5, and about the origin of viral haemoproteins in general.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coinfection with chikungunya and Zika results in mild disease and distinct inflammatory response. 同时感染基孔肯雅热和寨卡病毒会导致轻微的疾病和明显的炎症反应。

Npj viruses Pub Date : 2025-02-11 DOI: 10.1038/s44298-025-00098-w

Juliana Cardoso Alves, Lucas Sousa Magalhães, Priscila Lima Dos Santos, Regina Adalva de Lucena Couto Ócea, Alejandra Debbo, Jaira Vanessa de Carvalho, Mauro Martins Teixeira, Suresh Mahalingam, Amelia Ribeiro de Jesus, Angela Maria da Silva, Roque Pacheco de Almeida, Camilla Natália Oliveira Santos

{"title":"Coinfection with chikungunya and Zika results in mild disease and distinct inflammatory response.","authors":"Juliana Cardoso Alves, Lucas Sousa Magalhães, Priscila Lima Dos Santos, Regina Adalva de Lucena Couto Ócea, Alejandra Debbo, Jaira Vanessa de Carvalho, Mauro Martins Teixeira, Suresh Mahalingam, Amelia Ribeiro de Jesus, Angela Maria da Silva, Roque Pacheco de Almeida, Camilla Natália Oliveira Santos","doi":"10.1038/s44298-025-00098-w","DOIUrl":"https://doi.org/10.1038/s44298-025-00098-w","url":null,"abstract":"Chikungunya (CHIKV) and Zika (ZIKV) viruses, both mosquito-borne, often circulate simultaneously, raising concerns about the effects of coinfection. This study evaluated cytokines, chemokines, and growth factors in 12 patients with concurrent CHIKV and ZIKV infections confirmed by RT-qPCR. Clinical data and 45 immune mediators were analyzed. Coinfected and monoinfected patients exhibited similar symptoms, although ZIKV-infected individuals experienced fewer instances of fever. No patients had persistent symptoms or required hospitalization. Chemokines CCL5, CXCL1, and CXCL10 were elevated across all groups. CHIKV-infected patients showed higher levels of CCL2, CCL4, EGF, CXCL12, and IFN-α compared to controls, while IL-1RA, IL-8, and IFN-γ were elevated in both CHIKV and coinfected groups. SCF was elevated only in the ZIKV group. Overall, CHIKV and ZIKV coinfection presented mild clinical symptoms similar to monoinfections and demonstrated a moderate inflammatory response.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An XBB.1.5-based inactivated SARS-CoV-2 vaccine partially protects against XBB.1.5 and JN.1 strains in hamsters. 基于XBB.1.5的灭活SARS-CoV-2疫苗对仓鼠XBB.1.5和JN.1株有部分保护作用。

Npj viruses Pub Date : 2025-02-03 DOI: 10.1038/s44298-025-00096-y

Ryuta Uraki, Mutsumi Ito, Maki Kiso, Kiyoko Iwatsuki-Horimoto, Masafumi Endo, Seiya Yamayoshi, Yoshihiro Kawaoka

引用次数: 0

Dose and strain dependent lethality of Usutu virus in an Ifnar^-/- mouse model. Usutu病毒在Ifnar-/-小鼠模型中的剂量和毒株依赖性致死性。

Npj viruses Pub Date : 2025-01-28 DOI: 10.1038/s44298-025-00089-x

Johanna M Duyvestyn, Eleanor M Marshall, Peter J Bredenbeek, Barry Rockx, Martijn J van Hemert, Marjolein Kikkert

{"title":"Dose and strain dependent lethality of Usutu virus in an Ifnar-/- mouse model.","authors":"Johanna M Duyvestyn, Eleanor M Marshall, Peter J Bredenbeek, Barry Rockx, Martijn J van Hemert, Marjolein Kikkert","doi":"10.1038/s44298-025-00089-x","DOIUrl":"https://doi.org/10.1038/s44298-025-00089-x","url":null,"abstract":"Usutu virus (USUV) is a mosquito-borne zoonotic flavivirus with a geographic range that has expanded over recent years. Maintained in a transmission cycle between mosquito vectors and avian reservoirs the virus can cause large seasonal outbreaks in bird populations, but spillover into mammalian hosts has also been reported. While usually mild or asymptomatic in humans, neurological disorders are increasingly observed, which has boosted interest and the need for better understanding of the pathogenesis of various USUV lineages. In this study we inoculated interferon α/β receptor knockout (Ifnar-/-) mice with decreasing doses of USUV, monitoring symptoms and survival to determine a less lethal dose, and we directly compared isolates from three different viral lineages. We found that a Dutch isolate of USUV Africa-3 lineage is lethal at a dose of 20 pfu per mouse, which is considerably lower than what was anticipated based upon the literature. A Europe-2 strain showed an even higher virulence in this mouse model, compared to strains from Africa-3 and Europe-3 lineages-though this was not reflected in in vitro studies. These results enhance our understanding of the pathogenicity of different USUV strains and provide guidance for the use of low doses for inoculation in an Ifnar-/- animal model.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotoxic consequences of viral infections. 病毒感染的基因毒性后果。

Npj viruses Pub Date : 2025-01-27 DOI: 10.1038/s44298-024-00087-5

Olga Szewczyk-Roszczenko, Piotr Roszczenko, Yegor Vassetzky, Nikolajs Sjakste

引用次数: 0