Npj viruses最新文献_第2页

Experimental Usutu virus infection in Eurasian blackbirds (Turdus merula). 欧亚黑鸟（Turdus merula）中Usutu病毒的实验感染。

Npj viruses Pub Date : 2025-06-20 DOI: 10.1038/s44298-025-00133-w

Gianfilippo Agliani, Imke Visser, Eleanor M Marshall, Giuseppe Giglia, Erwin de Bruin, Ruben Verstappen, Tjomme van Mastrigt, Felicity Chandler, Reina Sikkema, Henk van der Jeugd, Marion P G Koopmans, Andrea Gröne, Barry Rockx, Judith M A van den Brand

{"title":"Experimental Usutu virus infection in Eurasian blackbirds (Turdus merula).","authors":"Gianfilippo Agliani, Imke Visser, Eleanor M Marshall, Giuseppe Giglia, Erwin de Bruin, Ruben Verstappen, Tjomme van Mastrigt, Felicity Chandler, Reina Sikkema, Henk van der Jeugd, Marion P G Koopmans, Andrea Gröne, Barry Rockx, Judith M A van den Brand","doi":"10.1038/s44298-025-00133-w","DOIUrl":"10.1038/s44298-025-00133-w","url":null,"abstract":"Usutu virus (USUV) is a zoonotic arbovirus causing disease in wild birds and humans. Eurasian blackbirds (Turdus merula) are highly susceptible to infection, developing severe lesions with high mortality rates during outbreaks. The pathogenesis and clinical course of the acute disease in birds are not known. Therefore, six blackbirds were infected with two USUV lineages (Africa 3 and Europe 3). The blackbirds were monitored for clinical signs of disease and activity levels and were sampled for virus detection. All infected blackbirds showed severe disease after 4 to 7 days, with no significant differences between the lineages. Reaching a humane endpoint, the blackbirds were euthanized, and tissue samples were collected for histopathology and virology. At histopathology, lesions were seen in the main target organs with the associated presence of infectious virus and viral antigen. In conclusion, blackbirds can be infected experimentally with USUV and show similar disease as seen in natural infection.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"51"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential patterns of cross-protection against antigenically distinct variants in small animal models of SARS-CoV-2 infection. 在SARS-CoV-2感染的小动物模型中，针对不同抗原变体的交叉保护的不同模式

Npj viruses Pub Date : 2025-06-04 DOI: 10.1038/s44298-025-00125-w

Prabhuanand Selvaraj, Charles B Stauft, Shufeng Liu, Kotou Sangare, Tony T Wang

引用次数: 0

Ultrastructural and transcriptional changes during a giant virus infection of a green alga. 巨型病毒感染绿藻时超微结构和转录的变化。

Npj viruses Pub Date : 2025-05-31 DOI: 10.1038/s44298-025-00128-7

Andrian P Gajigan, Christopher R Schvarcz, Cecilia Conaco, Kyle F Edwards, Grieg F Steward

引用次数: 0

A deep dive into giant viruses. 深入研究巨型病毒。

Npj viruses Pub Date : 2025-05-31 DOI: 10.1038/s44298-025-00131-y

Jônatas Santos Abrahão

引用次数: 0

Influenza A virus NS1 suppresses nuclear speckles promoted gene expression by inhibition of transcription. 甲型流感病毒NS1通过抑制转录抑制核斑点促进基因表达。

Npj viruses Pub Date : 2025-05-30 DOI: 10.1038/s44298-025-00124-x

Wolfgang Nacken, Juliane Mayr, André Schreiber, Stephan Ludwig

引用次数: 0

Nonviral protein cages as tools to decipher and combat viral threats. 非病毒蛋白笼作为破译和对抗病毒威胁的工具。

Npj viruses Pub Date : 2025-05-26 DOI: 10.1038/s44298-025-00127-8

Mikail D Levasseur

引用次数: 0

Enhanced neurotropism of bovine H5N1 compared to the Vietnam H5N1 isolate in C57BL/6J mice. 牛H5N1在C57BL/6J小鼠中的嗜神经性增强与越南H5N1分离株的比较

Npj viruses Pub Date : 2025-05-23 DOI: 10.1038/s44298-025-00121-0

Kerry Goldin, Sarah van Tol, Randall C Johnson, Reshma Koolaparambil Mukesh, Kendal G Cooper, Shane Gallogly, Jonathan E Schulz, Jessica Prado-Smith, Craig Martens, Greg Saturday, Kwe Claude Yinda, Vincent J Munster, Emmie de Wit, Neeltje van Doremalen

{"title":"Enhanced neurotropism of bovine H5N1 compared to the Vietnam H5N1 isolate in C57BL/6J mice.","authors":"Kerry Goldin, Sarah van Tol, Randall C Johnson, Reshma Koolaparambil Mukesh, Kendal G Cooper, Shane Gallogly, Jonathan E Schulz, Jessica Prado-Smith, Craig Martens, Greg Saturday, Kwe Claude Yinda, Vincent J Munster, Emmie de Wit, Neeltje van Doremalen","doi":"10.1038/s44298-025-00121-0","DOIUrl":"10.1038/s44298-025-00121-0","url":null,"abstract":"In this study, we investigated differences in tissue tropism of two HPAI H5N1 strains, the isolate A/Vietnam/1203/2004 (VN1203) isolated from a fatal human case in 2004 and the bovine isolate A/Bovine/Ohio/B24osu-342/2024 (Bov342) isolated in 2024, in C57BL/6J mice. Infection via aerosols was uniformly lethal in mice. However, tissue tropism differed significantly: while VN1203 replication was largely restricted to the respiratory tract, Bov342 successfully replicated in the respiratory tract as well as various regions of the brain. Correspondingly, cytokine profiles in the brain differed significantly between the isolates. Notably, in addition to abundant evidence of CNS infection in Bov342-challenged mice via immunohistochemistry, sporadic intranuclear and intracytoplasmic immunoreactivity was observed in other tissues in the head, including the choroid plexus, retina, and inner ear. This study demonstrates that while both HPAI H5N1 isolates are uniformly lethal in C57BL/6J mice upon aerosol exposure, significant differences exist in tissue tropism.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Langat virus infectious clones as a platform for live-attenuated tick-borne encephalitis vaccine. Langat病毒感染性克隆的研制及其作为蜱传脑炎减毒活疫苗的平台。

Npj viruses Pub Date : 2025-05-23 DOI: 10.1038/s44298-025-00129-6

Naveed Asghar, Rita Jaafar, Anna Valko, Olivia Merinder, Karl Ljungberg, Carl Mårten Lindqvist, Magnus Johansson

引用次数: 0

Author Correction: Transmission dynamics of avian influenza viruses in Egyptian poultry markets. 作者更正：禽流感病毒在埃及家禽市场的传播动力学。

Npj viruses Pub Date : 2025-05-22 DOI: 10.1038/s44298-025-00130-z

Sara H Mahmoud, Mokhtar Gomaa, Ahmed El Taweel, Yassmin Moatasim, Mina Nabil Kamel, Mohamed El Sayes, Noura M Abo Shama, Rebecca Badra, Mona Mahmoud, Pamela P McKenzie, Richard J Webby, Ahmed Kandeil, Mohamed Ahmed Ali, Rabeh El-Shesheny, Ghazi Kayali

引用次数: 0

Characterization of A(H1N1)pdm09 influenza viruses isolated between 2016 and 2019. 2016年至2019年分离的甲型H1N1 pdm09流感病毒的特征

Npj viruses Pub Date : 2025-05-22 DOI: 10.1038/s44298-025-00126-9

Luthfi Muawan, Kosuke Takada, Sara Yoshimoto, Yurie Kida, Shinji Watanabe, Tokiko Watanabe

{"title":"Characterization of A(H1N1)pdm09 influenza viruses isolated between 2016 and 2019.","authors":"Luthfi Muawan, Kosuke Takada, Sara Yoshimoto, Yurie Kida, Shinji Watanabe, Tokiko Watanabe","doi":"10.1038/s44298-025-00126-9","DOIUrl":"10.1038/s44298-025-00126-9","url":null,"abstract":"The A(H1N1)pdm09 virus, which caused the 2009 influenza pandemic, has continued to circulate in humans for over a decade. Understanding its biological properties is crucial for effective surveillance, prevention, and control. Here, we characterized recently circulating A(H1N1)pdm09 viruses, focusing on strains isolated between 2016 and 2019. HA gene-based phylogenetic tree analysis revealed that post-pandemic A(H1N1)pdm09 virus strains circulating between 2016 and 2019 form two clusters: subclade 6B.1 and subclade 6B.1 A.5a. Growth kinetics of nine selected representative strains from these clusters showed that subclade 6B.1 viruses replicated well in human lung cells, whereas some subclade 6B.1 A.5a viruses replicated poorly. In vivo, all viruses from both subclades caused significantly less weight loss in infected mice compared to the prototypic pandemic strain A/California/04/2009 (Cal04/2009). Additionally, virus titers in the lungs of mice infected with most viruses from subclade 6B.1 or 6B.1 A.5a were significantly lower than those in mice infected with Cal04/2009. Furthermore, evolutionary analysis suggested multiple transitions to a less pathogenic phenotype, indicating an evolutionary trend towards attenuation. These results demonstrate that A(H1N1)pdm09 viruses isolated between 2016 and 2019 are attenuated in mice, although the mutations responsible for this attenuation require further investigation. Our findings emphasize the need for continued monitoring of A(H1N1)pdm09 viruses to understand their evolutionary dynamics and potential impact on public health.","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"42"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0