Kerry Goldin, Sarah van Tol, Randall C Johnson, Reshma Koolaparambil Mukesh, Kendal G Cooper, Shane Gallogly, Jonathan E Schulz, Jessica Prado-Smith, Craig Martens, Greg Saturday, Kwe Claude Yinda, Vincent J Munster, Emmie de Wit, Neeltje van Doremalen
{"title":"牛H5N1在C57BL/6J小鼠中的嗜神经性增强与越南H5N1分离株的比较","authors":"Kerry Goldin, Sarah van Tol, Randall C Johnson, Reshma Koolaparambil Mukesh, Kendal G Cooper, Shane Gallogly, Jonathan E Schulz, Jessica Prado-Smith, Craig Martens, Greg Saturday, Kwe Claude Yinda, Vincent J Munster, Emmie de Wit, Neeltje van Doremalen","doi":"10.1038/s44298-025-00121-0","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, we investigated differences in tissue tropism of two HPAI H5N1 strains, the isolate A/Vietnam/1203/2004 (VN1203) isolated from a fatal human case in 2004 and the bovine isolate A/Bovine/Ohio/B24osu-342/2024 (Bov342) isolated in 2024, in C57BL/6J mice. Infection via aerosols was uniformly lethal in mice. However, tissue tropism differed significantly: while VN1203 replication was largely restricted to the respiratory tract, Bov342 successfully replicated in the respiratory tract as well as various regions of the brain. Correspondingly, cytokine profiles in the brain differed significantly between the isolates. Notably, in addition to abundant evidence of CNS infection in Bov342-challenged mice via immunohistochemistry, sporadic intranuclear and intracytoplasmic immunoreactivity was observed in other tissues in the head, including the choroid plexus, retina, and inner ear. This study demonstrates that while both HPAI H5N1 isolates are uniformly lethal in C57BL/6J mice upon aerosol exposure, significant differences exist in tissue tropism.</p>","PeriodicalId":520240,"journal":{"name":"Npj viruses","volume":"3 1","pages":"43"},"PeriodicalIF":0.0000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102188/pdf/","citationCount":"0","resultStr":"{\"title\":\"Enhanced neurotropism of bovine H5N1 compared to the Vietnam H5N1 isolate in C57BL/6J mice.\",\"authors\":\"Kerry Goldin, Sarah van Tol, Randall C Johnson, Reshma Koolaparambil Mukesh, Kendal G Cooper, Shane Gallogly, Jonathan E Schulz, Jessica Prado-Smith, Craig Martens, Greg Saturday, Kwe Claude Yinda, Vincent J Munster, Emmie de Wit, Neeltje van Doremalen\",\"doi\":\"10.1038/s44298-025-00121-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, we investigated differences in tissue tropism of two HPAI H5N1 strains, the isolate A/Vietnam/1203/2004 (VN1203) isolated from a fatal human case in 2004 and the bovine isolate A/Bovine/Ohio/B24osu-342/2024 (Bov342) isolated in 2024, in C57BL/6J mice. Infection via aerosols was uniformly lethal in mice. However, tissue tropism differed significantly: while VN1203 replication was largely restricted to the respiratory tract, Bov342 successfully replicated in the respiratory tract as well as various regions of the brain. Correspondingly, cytokine profiles in the brain differed significantly between the isolates. Notably, in addition to abundant evidence of CNS infection in Bov342-challenged mice via immunohistochemistry, sporadic intranuclear and intracytoplasmic immunoreactivity was observed in other tissues in the head, including the choroid plexus, retina, and inner ear. This study demonstrates that while both HPAI H5N1 isolates are uniformly lethal in C57BL/6J mice upon aerosol exposure, significant differences exist in tissue tropism.</p>\",\"PeriodicalId\":520240,\"journal\":{\"name\":\"Npj viruses\",\"volume\":\"3 1\",\"pages\":\"43\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102188/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Npj viruses\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/s44298-025-00121-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Npj viruses","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s44298-025-00121-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Enhanced neurotropism of bovine H5N1 compared to the Vietnam H5N1 isolate in C57BL/6J mice.
In this study, we investigated differences in tissue tropism of two HPAI H5N1 strains, the isolate A/Vietnam/1203/2004 (VN1203) isolated from a fatal human case in 2004 and the bovine isolate A/Bovine/Ohio/B24osu-342/2024 (Bov342) isolated in 2024, in C57BL/6J mice. Infection via aerosols was uniformly lethal in mice. However, tissue tropism differed significantly: while VN1203 replication was largely restricted to the respiratory tract, Bov342 successfully replicated in the respiratory tract as well as various regions of the brain. Correspondingly, cytokine profiles in the brain differed significantly between the isolates. Notably, in addition to abundant evidence of CNS infection in Bov342-challenged mice via immunohistochemistry, sporadic intranuclear and intracytoplasmic immunoreactivity was observed in other tissues in the head, including the choroid plexus, retina, and inner ear. This study demonstrates that while both HPAI H5N1 isolates are uniformly lethal in C57BL/6J mice upon aerosol exposure, significant differences exist in tissue tropism.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
