Pediatric discovery最新文献

{"title":"Human Umbilical Cord Mesenchymal Stem Cell-Derived Microvesicles Alleviate Bronchopulmonary Dysplasia in Preterm Rats by Inhibiting Neutrophil Extracellular Trap Formation.","authors":"Yi Lu, Wen Tan, Xiao Yang, Tingting Luo, Chenghao Mei, Huaqin Bu, Jinhua Ma, Chao Gong, Li Zhong, Ou Zhou, Daiyin Tian","doi":"10.1002/pdi3.70065","DOIUrl":"10.1002/pdi3.70065","url":null,"abstract":"<p><p>Among preterm infants, bronchopulmonary dysplasia (BPD) is the most prevalent chronic pulmonary disorder. Its pathogenesis involves a complex interplay between prenatal inflammatory exposure and abnormal immune activation. Neutrophil extracellular traps (NETs) are mediators of tissue damage, but whether they bridge intrauterine inflammation and the subsequent development of BPD is not yet clear. We used a prenatal lipopolysaccharide (LPS)-exposed rat model to ask two questions: first, whether excessive NETs drive BPD pathogenesis, and second, whether human umbilical cord mesenchymal stem cell-derived microvesicles (hUCMSC-MVs) can alleviate lung injury by inhibiting this process. We established a BPD model in preterm rats via prenatal LPS exposure and assessed lung morphometry, neutrophil infiltration, and NETs markers. We then compared the therapeutic effects of hUCMSC-MVs with NET extrusion (NETosis) inhibitors in vivo and also examined direct neutrophil effects in vitro. Prenatal LPS exposure led to significantly elevated NET markers in both lung tissue and circulation, which correlated with severe alveolar simplification. Neutrophils in this model exhibited a \"primed\" phenotype with enhanced potential for NETs release. In vivo, hUCMSC-MVs suppressed NET formation, attenuated neutrophilic inflammation, and restored alveolar structure, matching the efficacy of conventional NETosis inhibitors. In vitro, they were internalized by neutrophils and abrogated LPS-induced NETosis. These findings point to excessive NETosis as a key mechanistic link between prenatal inflammation and postnatal lung impairment. hUCMSC-MVs directly inhibit this pathological process and alleviate BPD-like lung injury. This work advances the mechanistic understanding of BPD and supports hUCMSC-MVs as a promising cell-free therapeutic candidate.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":" ","pages":"e70065"},"PeriodicalIF":0.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148687426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucocorticoids Impair the Airway Epithelial Barrier via Autophagy-Associated Apoptosis in Asthma: A Preliminary Study Both In Vitro and In Vivo.","authors":"Hao You, Wenjing Zou, Tangqiaochu Gan, Yu Zhao, Jie Hu, Wen Tan, Ting Wang, Luo Ren, Zhengxiu Luo, Zhou Fu, Chao Niu","doi":"10.1002/pdi3.70060","DOIUrl":"10.1002/pdi3.70060","url":null,"abstract":"<p><p>Glucocorticoid insensitivity affects a subset of asthma patients, yet its mechanism is unclear. Given the key role of airway epithelium in asthma, this study hypothesized that glucocorticoids may impair this barrier. We examined whether glucocorticoid-induced autophagy-associated apoptosis contributes to reduced treatment efficacy. Using an ovalbumin-induced asthma model in BALB/c mice and 16HBE human bronchial epithelial cells treated with dexamethasone and/or autophagy inhibitor EACC (C₁₃H₁₁N₃O₆S₂), we assessed cell viability and apoptosis via CCK-8 and TUNEL assays, respectively. Apoptosis and autophagy markers were analyzed by quantitative polymerase chain reaction (PCR) and western blot. Dexamethasone alleviated airway inflammation but worsened epithelial integrity in asthmatic mice. It failed to downregulate pro-apoptotic factors while reducing anti-apoptotic factors both in vivo and in vitro. Dexamethasone decreased 16HBE cell viability and increased apoptosis. Mechanistically, dexamethasone upregulated autophagy markers and suppressed anti-autophagy factors in mouse lungs and cells, concurrently reducing the phosphorylation of negative regulators of autophagy. Importantly, the autophagy inhibitor EACC enhanced cell viability and attenuated dexamethasone-induced apoptotic signaling in 16HBE cells. These findings suggest glucocorticoids may compromise the airway epithelial barrier via autophagy-associated apoptosis in vitro and in vivo, indicating autophagy inhibition as a potential hypothesis for future therapeutic exploration for glucocorticoid-insensitive asthma.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":" ","pages":"e70060"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-130a-3p Targets the Androgen-Related Transcription Factor <i>MAFB</i>: Effects on Proliferation, Migration, Apoptosis, and Cell Cycle in Hypospadias.","authors":"Jiaxin Zhou, Yixuan Wang, Zhicheng Zhang, Qiang Zhang, Zhenmin Liu, Hongsong Chen, Xingguo Luo, Chunlan Long, Lianju Shen, Xing Liu, Guanghui Wei","doi":"10.1002/pdi3.70062","DOIUrl":"10.1002/pdi3.70062","url":null,"abstract":"<p><p>Hypospadias is among the most frequent congenital malformations of the male genitalia. The expression of <i>V-maf musculoaponeurotic fibrosarcoma oncogene homolog B</i> (<i>MAFB</i>), a transcription factor within the androgen signaling pathway, is closely correlated with the etiology of this condition. From an epigenetic perspective, this study investigated the role of microRNA-130a-3p (miR-130a-3p) in regulating <i>MAFB</i> during hypospadias pathogenesis. Clinical foreskin samples from hypospadias children and the dioctyl phthalate (DEHP)-induced mouse genital tubercle tissues were analyzed. The expression levels of miR-130a-3p and <i>MAFB</i> were detected by qRT-PCR and western blot. In vitro, miR-130a-3p was modulated in HS68 cells; CCK-8, scratch assay, western blot, flow cytometry, and dual-luciferase assay were used to assess its effects and targeting of <i>MAFB</i>. Our study demonstrated that miR-130a-3p targeted and suppressed <i>MAFB</i> expression. This suppression inhibited cellular proliferation and migration and reduced <i>Vimentin</i> mRNA expression. Cell cycle distribution was disrupted, marked by Gap 1 phase increase and Synthesis phase reduction, and the cell apoptosis rate increased. Cyclin-dependent kinase 2 (CDK2), Cyclin E1, and Proliferating Cell Nuclear Antigen (PCNA) were downregulated. miR-130a-3p targets and inhibits <i>MAFB</i> expression, disrupting the normal processes of cell proliferation, migration, cell apoptosis, and cell cycle progression, ultimately leading to the development of hypospadias.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":" ","pages":"e70062"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148586575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HucMSC-Derived Exosomes Preserve Mafb-Dependent Tubular Epithelial Identity and Suppress Dedifferentiation in Obstructive Nephropathy.","authors":"Zhuocheng Shi, Chenxi Jia, Meiling Chen, Yihang Yu, Deying Zhang, Guanghui Wei","doi":"10.1002/pdi3.70059","DOIUrl":"10.1002/pdi3.70059","url":null,"abstract":"<p><p>Tubular epithelial dedifferentiation and loss of polarity are early pathological events in obstructive nephropathy and critically contribute to progressive renal fibrosis. Human umbilical cord mesenchymal stem cell-derived exosomes (HucMSC-Exos) have shown therapeutic potential for kidney injury, yet the mechanisms by which they preserve epithelial identity remain unclear. Mafb, a transcription factor essential for renal epithelial differentiation, has been implicated in epithelial homeostasis, but its role in adult obstructive injury and its regulation by exosomes are unknown. Here, a unilateral ureteral obstruction (UUO) mouse model was used to evaluate early tubular injury and the effects of HucMSC-Exos. UUO induced marked epithelial damage characterized by decreased E-cadherin, increased vimentin and α-smooth muscle actin (α-SMA) expression, and disruption of epithelial polarity, whereas HucMSC-Exo administration significantly ameliorated these alterations. Mafb expression was profoundly downregulated in UUO kidneys but was effectively restored following exosome treatment. In vitro, <i>Mafb</i> knockdown in Human renal proximal tubular epithelial (HK-2) cells markedly aggravated TGF-β1-induced dedifferentiation, confirming its protective role in epithelial integrity. Analysis of <i>Mafb</i> ^-/- embryonic kidneys demonstrated severe developmental defects and loss of polarity markers, highlighting the essential function of <i>Mafb</i> in renal epithelium. Importantly, HucMSC-Exos rescued epithelial marker expression even in <i>Mafb</i>-silenced cells, suggesting both <i>Mafb</i>-dependent and independent mechanisms. These findings identify <i>Mafb</i> as a key regulator of tubular epithelial homeostasis and reveal that HucMSC-Exos attenuate early renal injury partly through Mafb restoration.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":" ","pages":"e70059"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148586599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Attenuate Renal Fibrosis by Suppressing Fibroblast Activation via the INHBA/PI3K/AKT Pathway.","authors":"Meiling Chen, Chenxi Jia, Zhuocheng Shi, Bianan Zhu, Yihang Yu, Xing Liu, Feng Liu, Jianwei Chen, Tao Xu, Yuanyuan Zhang, Deying Zhang, Guanghui Wei","doi":"10.1002/pdi3.70061","DOIUrl":"10.1002/pdi3.70061","url":null,"abstract":"<p><p>Renal fibrosis is a common pathological feature and key driver of progression to end-stage renal disease in various chronic kidney diseases, with effective treatments remaining scarce. Exosomes derived from mesenchymal stem cells (MSC-Exos) have demonstrated tremendous potential in tissue and organ repair and antifibrosis treatment. This study investigated the therapeutic effects and mechanisms of MSC-Exo derived from human umbilical cord (HucMSC-Exo) on renal fibrosis. HucMSC-Exo was applied to intervene in a mouse model of renal fibrosis induced by unilateral ureteral obstruction (UUO) or cocultured with TGF-β-stimulated rat renal fibroblasts (NRK-49F). Results showed that HucMSC-Exo conspicuously alleviated pathological damage, inflammatory response, fibroblast proliferation/activation, and extracellular matrix deposition in UUO kidneys. Single-cell sequencing revealed a prominent upregulation of <i>Inhba</i> gene in UUO kidneys, whereas HucMSC-Exo treatment effectively inhibited its expression. Further mechanistic studies showed that knocking down <i>Inhba</i> mimicked the antifibrotic effects of HucMSC-Exo, whereas exogenously adding INHBA weakened its protective effects. Transcriptome sequencing results revealed that the downstream effects of <i>Inhba</i> involve regulating the PI3K/AKT signaling pathway, and HucMSC-Exo treatment markedly inhibited the activation of this pathway. Collectively, HucMSC‑Exo exerts antifibrotic effects by regulating the INHBA/PI3K/AKT signaling axis to inhibit renal fibroblast activation.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":" ","pages":"e70061"},"PeriodicalIF":0.0,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148586622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mild Malnutrition in Children Aged 6-24 Months and Related Influencing Factors: Research From an Outpatient Clinic of Pediatric Health Care.","authors":"Menghua Yin, Qian Li, Rumeng Yu, Mengxuan Zhou, Wenxiu Wu, Li Song","doi":"10.1002/pdi3.70058","DOIUrl":"10.1002/pdi3.70058","url":null,"abstract":"<p><p>Mild malnutrition in infants and young children is an early stage of nutritional deficiency characterized by a slight reduction in nutritional reserves and mild impairment of physiological functions but no obvious clinical symptoms or severe growth retardation. Parents often overlook this condition because typical signs are absent. This cross-sectional study aimed to investigate the current status of mild malnutrition in infants and young children aged 6-24 months attending a pediatric health clinic, analyze its influencing factors, and provide a basis for nutritional health guidance. The study included 879 infants and young children aged 6-24 months who underwent physical examinations at the Outpatient Clinic of Pediatric Health Care, The Third Affiliated Hospital of Zhengzhou University, China, from December 2024 to August 2025. The survey results showed a detection rate of mild malnutrition of 13.88% (122/879). After including statistically significant factors from univariable analysis in a multivariable logistic regression, the following risk factors were identified: food allergies (odds ratio (OR) = 3.117, 95% confidence interval (CI): 1.902-5.108), low maternal education level (OR = 1.969, 95% CI: 1.166-3.325), low birth weight (OR = 1.759, 95% CI: 1.041-2.973), maternal prepregnancy BMI < 18.5 (OR = 2.197, 95% CI: 1.252-3.855), frequent nighttime awakenings (OR = 2.427, 95% CI: 1.481-3.975), and insufficient complementary feeding frequency (OR = 1.550, 95% CI: 1.030-2.332). The detection rate of mild malnutrition in infants and young children aged 6-24 months at pediatric clinics is relatively high, and clinical work should give sufficient attention to this issue. Proactive guidance should be provided to these children to promote healthy growth.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 2","pages":"e70058"},"PeriodicalIF":0.0,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Single-Cell Transcriptomic Profiling Reveals Dynamic Immune Cell Alterations During Burosumab Therapy in X-Linked Hypophosphatemia.","authors":"Yue Xie, Li Li, Rong Li, Yihong Sun, Ting Zhou, Yupeng Cun, Gaohui Zhu","doi":"10.1002/pdi3.70053","DOIUrl":"10.1002/pdi3.70053","url":null,"abstract":"<p><p>X-linked hypophosphatemia (XLH) is a rare hereditary disorder characterized by <i>PHEX</i> gene mutations, elevated <i>FGF23</i> levels, and impaired bone mineralization. Burosumab, a monoclonal antibody targeting <i>FGF23</i>, has demonstrated clinical efficacy; however, the immunological dynamics during treatment remain unexplored. This study employed longitudinal single-cell RNA sequencing (scRNA-seq) to characterize peripheral blood immune cell alterations across multiple treatment stages in pediatric XLH. We performed scRNA-seq on peripheral blood mononuclear cells from pediatric patients with XLH at five time points spanning pretreatment and burosumab therapy phases, along with healthy pediatric controls. A total of 93,112 cells were analyzed using comprehensive bioinformatic pipelines, including unsupervised clustering, pseudotime trajectory analysis, temporal gene expression profiling, and cell-cell communication inference. Eleven major immune cell populations were identified, with notable dynamic alterations in T cells and natural killer (NK) cell subtypes across treatment stages. The cellular proportion of T helper 2 (Th2) cells and regulatory T (Treg) cells were elevated before treatment and normalized during therapy, whereas T helper 17 (Th17) cells exhibited reciprocal patterns. Genes upregulated in Treg cells during early treatment were enriched in osteoclast differentiation pathway. Natural killer subtype 2 cells showed enrichment in osteoclast differentiation and interleukin-12 response pathways. Cell-cell communication analysis identified dynamic interactions among Th2 cells, Th17 cells, Treg cells, and NK cell subtypes mediated by KLRB1-CLEC2D and SELL-SELPLG ligand-receptor pairs. This longitudinal transcriptomic study provides the first comprehensive characterization of peripheral immune dynamics during burosumab therapy in XLH, offering new insights into the immunological mechanisms underlying treatment response.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 2","pages":"e70053"},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediatric Cardiac Function Staging and Management Recommendations.","authors":"Bo Pan, Ling Han, Jianxin Zhuang, Xing Shen, Chenggang Mao, Yao Lin, Jindou An, Huili Zhang, Chunhong Xie, Yanmin Zhang, Li Zhang, Ying Guo, Yanyan Xiao, Shiwei Yang, Lei Zhang, Yue Yuan, Ling Sun, Zhi Chen, Wenhong Ding, Yifei Li, Zipu Li, Jie Tian","doi":"10.1002/pdi3.70063","DOIUrl":"10.1002/pdi3.70063","url":null,"abstract":"<p><p>The global prevalence of heart failure in children continues to increase, accompanied by a growing disease burden. To advance early prevention and intervention by shifting the focus upstream in the disease process, the Cardiovascular Group of the Pediatrics Society of the Chinese Medical Association has introduced the concept of pediatric cardiac function staging applicable to all children. This framework categorizes pediatric cardiac function into three distinct stages: the stage of cardiac function sufficiency (Stage A), the stage of cardiac dysfunction (Stage B), and the stage of cardiac function failure (Stage C). Based on this staging system, the diagnostic criteria and management strategies for each stage are delineated, with the aim of establishing a comprehensive management pathway rooted in cardiac function staging. This approach seeks to further reduce the incidence and burden of pediatric heart failure, providing a theoretical foundation and practical guidance for achieving the goal of making pediatric heart failure preventable, manageable, and treatable.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 2","pages":"e70063"},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Salivary Micro Ribonucleic Acid Levels With the Severity of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Children.","authors":"Steven D Hicks, Dongxiao Zhu, Nirupama Kannikeswaran, Kathleen Meert, Wei Chen, Rhea Sullivan, Srinivasan Suresh, Usha Sethuraman","doi":"10.1002/pdi3.70050","DOIUrl":"10.1002/pdi3.70050","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may impact immune-related micro ribonucleic acids (miRNAs). The role of salivary miRNAs as predictors of severe infection in children is unknown. This study sought to examine the relationship between SARS-CoV-2 severity and salivary miRNA levels in children. A convenience sample of 400 children with SARS-CoV-2 infection was prospectively evaluated between March 2021 and February 2022 at two U.S. children's hospitals. Saliva swabs were obtained. Levels of 3 miRNAs previously implicated in SARS-CoV-2 pathophysiology were measured with quantitative polymerase chain reaction (miR-1273c, miR-296-5p, and miR-4495). The primary outcome measure was the occurrence of severe illness, including respiratory failure, shock, or death from a related cause within 1 month of evaluation. Disease resolution was determined through chart review and caretaker phone calls 4 weeks after the initial visit. Saliva samples from 395 (severe = 105) participants (mean age: 7.4 ± 5.9 years, 51.7% female, 54.7% African American) were analyzed. Children with severe outcomes had lower levels of miR-296-5p (fold difference: 0.361, <i>d</i> = 0.111, <i>p</i> = 0.046). Multivariable adjusted logistic regression analysis demonstrated an inverse association between severe outcomes and levels of miR-296-5p (adjusted odds ratio = 0.89; 95% confidence interval [0.81, 0.98], <i>p</i> = 0.021) while controlling for age, sex, race, weight, insurance, diabetes, asthma, fever, and symptom duration. The model had an area under curve of 0.744 (sensitivity = 0.71, specificity = 0.66). Measurement of saliva miRNA in conjunction with demographic and clinical characteristics may aid in the prediction of severe illness. Larger, longitudinal studies to assess the utility of salivary miRNAs in SARS-CoV-2 and other viral infections are needed.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 2","pages":"e70050"},"PeriodicalIF":0.0,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neonatal Advanced Practice Nursing: Lessons From American Academy of Pediatrics Clinical Report to the Global South.","authors":"Aditya Hemendra Bhatt, Somashekhar Marutirao Nimbalkar","doi":"10.1002/pdi3.70057","DOIUrl":"10.1002/pdi3.70057","url":null,"abstract":"","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 2","pages":"e70057"},"PeriodicalIF":0.0,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}