The Plant Pathology Journal最新文献

Trunk Injection of Citrus Trees with a Polymeric Nanobactericide Reduces Huanglongbing Severity Caused by Candidatus Liberibacter asiaticus. 向柑橘树树干注射聚合纳米杀菌剂可降低黄龙病菌引起的黄龙病严重程度

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.12.2023.0165

Ramiro Guerrero-Santos, Gabriela Cabrales-Orona, John Paul Délano-Frier, Judith Cabello-Romero, José Román Torres-Lubián, José Humberto Valenzuela-Soto

{"title":"Trunk Injection of Citrus Trees with a Polymeric Nanobactericide Reduces Huanglongbing Severity Caused by Candidatus Liberibacter asiaticus.","authors":"Ramiro Guerrero-Santos, Gabriela Cabrales-Orona, John Paul Délano-Frier, Judith Cabello-Romero, José Román Torres-Lubián, José Humberto Valenzuela-Soto","doi":"10.5423/ppj.oa.12.2023.0165","DOIUrl":"https://doi.org/10.5423/ppj.oa.12.2023.0165","url":null,"abstract":"Huanglongbing (HLB) is a disease caused by the phloem- limited Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry worldwide. To date, only indirect strategies have been implemented to eradicate HLB. Included among these is the population control of the psyllid vector (Diaphorina citri), which usually provides inconsistent results. Even though strategies for direct CLas suppression seem a priori more promising, only a handful of reports have been focused on a confrontation of the pathogen. Recent developments in polymer chemistry have allowed the design of polycationic self-assembled block copolymers with outstanding antibacterial capabilities. Here, we report the use of polymeric nano-sized bactericide particles (PNB) to control CLas directly in the phloem vasculature. The field experiments were performed in Rioverde, San Luis Potosí, and is one of the most important citrusproducing regions in Mexico. An average 52% reduction in the bacterial population was produced when PNB was injected directly into the trunk of 20 infected trees, although, in some cases, reduction levels reached 97%. These results position PNB as a novel and promising nanotechnological tool for citrus crop protection against CLas and other related pathogens.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Revision of the Phylogeny of Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae) as Inferred from Ribosomal and Mitochondrial DNA. 从核糖体和线粒体 DNA 推断的 Helicotylenchus Steiner, 1945（天牛科：Hoplolaimidae）系统发育的修订。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0013

Abraham Okki Mwamula, Oh-Gyeong Kwon, Chanki Kwon, Yi Seul Kim, Young Ho Kim, Dong Woon Lee

{"title":"A Revision of the Phylogeny of Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae) as Inferred from Ribosomal and Mitochondrial DNA.","authors":"Abraham Okki Mwamula, Oh-Gyeong Kwon, Chanki Kwon, Yi Seul Kim, Young Ho Kim, Dong Woon Lee","doi":"10.5423/ppj.oa.01.2024.0013","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0013","url":null,"abstract":"Identification of Helicotylenchus species is very challenging due to phenotypic plasticity and existence of cryptic species complexes. Recently, the use of rDNA barcodes has proven to be useful for identification of Helicotylenchus. Molecular markers are a quick diagnostic tool and are crucial for discriminating related species and resolving cryptic species complexes within this speciose genus. However, DNA barcoding is not an error-free approach. The public databases appear to be marred by incorrect sequences, arising from sequencing errors, mislabeling, and misidentifications. Herein, we provide a comprehensive analysis of the newly obtained, and published DNA sequences of Helicotylenchus, revealing the potential faults in the available DNA barcodes. A total of 97 sequences (25 nearly full-length 18S-rRNA, 12 partial 28S-rRNA, 16 partial internal transcribed spacer [ITS]-rRNA, and 44 partial cytochrome c oxidase subunit I [COI] gene sequences) were newly obtained in the present study. Phylogenetic relationships between species are given as inferred from the analyses of 103 sequences of 18S-rRNA, 469 sequences of 28S-rRNA, 183 sequences of ITS-rRNA, and 63 sequences of COI. Remarks on suggested corrections of published accessions in GenBank database are given. Additionally, COI gene sequences of H. dihystera, H. asiaticus and the contentious H. microlobus are provided herein for the first time. Similar to rDNA gene analyses, the COI sequences support the genetic distinctness and validity of H. microlobus. DNA barcodes from type material are needed for resolving the taxonomic status of the unresolved taxonomic groups within the genus.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Resistance of Radish Cultivars against Bacterial Soft Rot Caused by Pectobacterium carotovorum subsp. carotovorum. 萝卜品种对由 Pectobacterium carotovorum subsp.

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0011

Soo Min Lee, Jin Ju Lee, Hun Kim, Gyung Ja Choi

{"title":"Differential Resistance of Radish Cultivars against Bacterial Soft Rot Caused by Pectobacterium carotovorum subsp. carotovorum.","authors":"Soo Min Lee, Jin Ju Lee, Hun Kim, Gyung Ja Choi","doi":"10.5423/ppj.oa.01.2024.0011","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0011","url":null,"abstract":"Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) is one of the most severe diseases in radish cultivation. To control this plant disease, the most effective method has been known to cultivate resistant cultivars. Previously, we developed an efficient bioassay method for investigating resistance levels with 21 resistant and moderately resistant cultivars of radish against a strain Pcc KACC 10421. In this study, our research expanded to investigate the resistance of radish cultivars against six Pcc strains, KACC 10225, KACC 10421, ATCC 12312, ATCC 15713, LY34, and ECC 301365. To this end, the virulence of the six Pcc strains was determined based on the development of bacterial soft rot in seedlings of four susceptible radish cultivars. The results showed that the Pcc strains exhibited different virulence in the susceptible cultivars. To explore the race differentiation of Pcc strains corresponding to the resistance in radish cultivars, we investigated the occurrence of bacterial soft rot caused by the six Pcc strains on the 21 resistant and moderate resistant cultivars. Our results showed that the average values of the area under the disease progress curve were positively correlated with the virulence of the strains and the number of resistant cultivars decreased as the virulence of Pcc strains increased. Taken together, our results suggest that the resistance to Pcc of the radish cultivars commercialized in Korea is more likely affected by the virulence of Pcc strains rather than by race differentiation of Pcc.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the Photosynthesis to Hormone Biosynthesis in Plants. 从植物的光合作用到激素的生物合成。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.rw.01.2024.0006

Hyong Woo Choi

引用次数: 0

Genetic Variation of Monilinia fructicola Population in Korea. 韩国果孢子菌种群的遗传变异。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0017

Su In Lee, Hwa-Jung Lee, Youn-Sig Kwak

{"title":"Genetic Variation of Monilinia fructicola Population in Korea.","authors":"Su In Lee, Hwa-Jung Lee, Youn-Sig Kwak","doi":"10.5423/ppj.oa.01.2024.0017","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0017","url":null,"abstract":"Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicol isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"271 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea. 韩国柑橘黄脉清病毒的发生和多重 PCR 检测。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.09.2023.0136

Taemin Jin, Ji-Kwang Kim, Hee-Seong Byun, Hong-Soo Choi, Byeongjin Cha, Hae-Ryun Kwak, Mikyeong Kim

{"title":"Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea.","authors":"Taemin Jin, Ji-Kwang Kim, Hee-Seong Byun, Hong-Soo Choi, Byeongjin Cha, Hae-Ryun Kwak, Mikyeong Kim","doi":"10.5423/ppj.oa.09.2023.0136","DOIUrl":"https://doi.org/10.5423/ppj.oa.09.2023.0136","url":null,"abstract":"Citrus yellow vein clearing virus (CYVCV) is a member of the Alphaflexiviridae family that causes yellow vein clearing symptoms on citrus leaves. A total of 118 leaf samples from nine regions of six provinces in Korea were collected from various citrus species in 2020 and 2021. Viral diagnosis using next-generation sequencing and reverse transcription polymerase chain reaction (RT-PCR) identified four viruses: citrus tristeza virus, citrus leaf blotch virus, citrus vein enation virus, and CYVCV. A CYVCV incidence of 9.3% was observed in six host plants, including calamansi, kumquat, Persian lime, and Eureka lemon. Among the citrus infected by CYVCV, only three samples showed a single infection; the other showed a mixed infection with other viruses. Eureka lemon and Persian lime exhibited yellow vein clearing, leaf distortion, and water-soak symptom underside of the leaves, while the other hosts showed only yellowing symptoms on the leaves. The complete genome sequences were obtained from five CYVCV isolates. Comparison of the isolates reported from the different geographical regions and hosts revealed the high sequence identity (95.2% to 98.8%). Phylogenetic analysis indicated that all the five isolates from Korea were clustered into same clade but were not distinctly apart from isolates from China, Pakistan, India, and Türkiye. To develop an efficient diagnosis system for the four viruses, a simultaneous detection method was constructed using multiplex RT-PCR. Sensitivity evaluation, simplex RT-PCR, and stability testing were conducted to verify the multiplex RT-PCR system developed in this study. This information will be useful for developing effective disease management strategies for citrus growers in Korea.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"271 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal Activity-Guided Analysis of Actinostemma lobatum Extracts through Serial Sub-fractions. 通过序列子馏分对放线菌提取物进行抗真菌活性指导分析

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.nt.11.2023.0152

Seonwoo Choi, Song Hee Lee, Byeong Su Hwang, Young Taek Oh, Junhyun Jeon

引用次数: 0

Chemical Composition and Biocontrol Activity of Different Essential Oils against Soil-Borne Fungal Pathogens. 不同精油对土传真菌病原体的化学成分和生物控制活性

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0002

Yusuf Akdeniz, Tuba Genç Kesimci

{"title":"Chemical Composition and Biocontrol Activity of Different Essential Oils against Soil-Borne Fungal Pathogens.","authors":"Yusuf Akdeniz, Tuba Genç Kesimci","doi":"10.5423/ppj.oa.01.2024.0002","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0002","url":null,"abstract":"In this study, the efficacy of the essential oil of Mentha longifolia, Achillea arabica and Artemisia absinthium plants were evaluated against important soil-borne fungal pathogens as Verticillium dahliae, Rhizoctonia solani, and Fusarium oxysporum. Essential oils were obtained from plants by hydrodistillation method and the chemical components of essential oils were determined by analyzing by gas chromatography-mass spectrometry. The main components found as piperitone oxide (13.61%), piperitenone oxide (15.55%), pulegone (12.47%), 1-menthone (5.75%), and camphor (5.75%) in M. longifolia, á-selinene 13.38%, camphor 13.34%, L-4-terpineneol 8.40%, (-)-á-Elemene 7.01%, 1,8-cineole 4.71%, and (-)-spathulenol 3.84% in A. arabica, and á-thujone (34.64%), 1,8-cineole (19.54%), pulegone (7.86%), camphene (5.31%), sabinene (4.86%), and germacrene-d (3.67%) in A. absinthium. The antifungal activities of the oils were investigated 0.05, 0.1, 0.25, 0.5, 1.00, and 2.00 μl/ml concentrations with the contact effect method. M. longifolia oil (1.00 and 2.00 μl/ml) has displayed remarkable antifungal effect and provided 100% inhibition on mycelial growth of V. dahliae, R. solani and F. oxysporum. The results obtained from this study may contribute to the development of new alternative and safe methods against soil-borne fungal pathogens.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea. 通过病毒元转录组学分析揭示韩国萨摩蜜柑（Citrus unshiu）病毒感染的多样性。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0009

Hae-Jun Kim, Se-Ryung Choi, In-Sook Cho, Rae-Dong Jeong

{"title":"Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea.","authors":"Hae-Jun Kim, Se-Ryung Choi, In-Sook Cho, Rae-Dong Jeong","doi":"10.5423/ppj.oa.01.2024.0009","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0009","url":null,"abstract":"Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"270 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Analysis Revealed Acholeplasma as a Possible Factor Influencing the Susceptibility to Bacterial Leaf Blight Disease of Two Domestic Rice Cultivars in Vietnam. 微生物组分析发现Acholeplasma可能是影响越南两个国内水稻品种对细菌性叶枯病敏感性的一个因素

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.nt.12.2023.0167

Thu Thi Hieu Nguyen, Cristina Bez, Iris Bertani, Minh Hong Nguyen, Thao Kim Nu Nguyen, Vittorio Venturi, Hang Thuy Dinh

{"title":"Microbiome Analysis Revealed Acholeplasma as a Possible Factor Influencing the Susceptibility to Bacterial Leaf Blight Disease of Two Domestic Rice Cultivars in Vietnam.","authors":"Thu Thi Hieu Nguyen, Cristina Bez, Iris Bertani, Minh Hong Nguyen, Thao Kim Nu Nguyen, Vittorio Venturi, Hang Thuy Dinh","doi":"10.5423/ppj.nt.12.2023.0167","DOIUrl":"https://doi.org/10.5423/ppj.nt.12.2023.0167","url":null,"abstract":"The microbiomes of two important rice cultivars in Vietnam which differ by their susceptibility to the bacterial leaf blight (BLB) disease were analyzed through 16S rRNA amplicon technology. A higher number of operational taxonomic units and alpha-diversity indices were shown in the BLB-resistant LA cultivar than in the BLB-susceptible TB cultivar. The BLB pathogen Xanthomonas was scantly found (0.003%) in the LA cultivar, whereas was in a significantly higher ratio in the TB cultivar (1.82%), reflecting the susceptibility to BLB of these cultivars. Of special interest was the genus Acholeplasma presented in the BLB-resistant LA cultivar at a high relative abundance (22.32%), however, was minor in the BLB-sensitive TB cultivar (0.09%), raising a question about its roles in controlling the Xanthomonas low in the LA cultivar. It is proposed that Acholeplasma once entered the host plant would hamper other phytopathogens, i.e. Xanthomonas, by yet unknown mechanisms, of which the triggering of the host plants to produce secondary metabolites against pathogens could be a testable hypothesis.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0