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Bacteriophage Cocktail Comprising Fifi044 and Fifi318 for Biocontrol of Erwinia amylovora. 由 Fifi044 和 Fifi318 组成的噬菌体鸡尾酒用于对 Erwinia amylovora 进行生物控制。

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.01.2024.0005

Byeori Kim, Seung Yeup Lee, Jungkum Park, Sujin Song, Kwang-Pyo Kim, Eunjung Roh

{"title":"Bacteriophage Cocktail Comprising Fifi044 and Fifi318 for Biocontrol of Erwinia amylovora.","authors":"Byeori Kim, Seung Yeup Lee, Jungkum Park, Sujin Song, Kwang-Pyo Kim, Eunjung Roh","doi":"10.5423/ppj.oa.01.2024.0005","DOIUrl":"https://doi.org/10.5423/ppj.oa.01.2024.0005","url":null,"abstract":"Erwinia amylovora is a plant pathogen that causes fire blight on apples and pears. Bacteriophages, which are viruses that selectively infect specific species of bacteria and are harmless to animal cells, have been considered as biological control agents for the prevention of bacterial pathogens. In this study, we aimed to use bacteriophages that infect E. amylovora as biocontrol agents against fire blight. We isolated bacteriophages Fifi044 and Fifi318 infecting E. amylovora, and characterized their morphology, plaque form, and genetic diversity to use as cocktails for disease control. The stabilities of the two phages were investigated at various temperatures and pH values and under sunlight, and long-term storage experiment was conducted for a year. To evaluate whether the two phages were suitable for use in cocktail form, growth curves of E. amylovora were prepared after treating the bacterial cells with single phages and a phage cocktail. In addition, a disease control test was conducted using immature apples and in vitro cultured apple plantlets to determine the biocontrol effects of the phage cocktail. The two phages were morphologically and genetically different, and highly stable up to 50°C and pH value from 4 to 10. The phages showed synergistic effect when used as a cocktail in the inhibition of host bacterial growth and the disease control. This study demonstrated that the potential of the phage cocktail as a biocontrol agent for commercial use.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Selective Medium for Surveillance of Fusarium Head Blight Disease. 开发用于监测镰刀菌头疫病的选择性培养基

The Plant Pathology Journal Pub Date : 2024-04-01 DOI: 10.5423/ppj.oa.12.2023.0173

Hosung Jeon, Jung Wook Yang, Donghwan Shin, Donggyu Min, Byung Joo Kim, Kyunghun Min, Hokyoung Son

{"title":"Development of a Selective Medium for Surveillance of Fusarium Head Blight Disease.","authors":"Hosung Jeon, Jung Wook Yang, Donghwan Shin, Donggyu Min, Byung Joo Kim, Kyunghun Min, Hokyoung Son","doi":"10.5423/ppj.oa.12.2023.0173","DOIUrl":"https://doi.org/10.5423/ppj.oa.12.2023.0173","url":null,"abstract":"Fusarium head blight (FHB), predominantly caused by Fusarium graminearum and F. asiaticum, is a significant fungal disease impacting small-grain cereals. The absence of highly resistant cultivars underscores the need for vigilant FHB surveillance to mitigate its detrimental effects. In 2023, a notable FHB outbreak occurred in the southern region of Korea. We assessed FHB disease severity by quantifying infected spikelets and grains. Isolating fungal pathogens from infected samples often encounters interference from various microorganisms. We developed a cost-effective, selective medium, named BGT (Burkholderia glumae Toxoflavin) medium, utilizing B. glumae, which is primarily known for causing bacterial panicle blight in rice. This medium exhibited selective growth properties, predominantly supporting Fusarium spp., while substantially inhibiting the growth of other fungi. Using the BGT medium, we isolated F. graminearum and F. asiaticum from infected wheat and barley samples across Korea. To further streamline the process, we used a direct PCR approach to amplify the translation elongation factor 1-α (TEF-1α) region without a separate genomic DNA extraction step. Phylogenetic analysis of the TEF-1α region revealed that the majority of the isolates were identified as F. asiaticum. Our results demonstrate that BGT medium is an effective tool for FHB diagnosis and Fusarium strain isolation.","PeriodicalId":519231,"journal":{"name":"The Plant Pathology Journal","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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