Comparative Immunology Reports最新文献

{"title":"Mandarin fish tankyrase 1-like protein regulates the Wnt/β-catenin pathway and facilitates the infectious spleen and kidney necrosis virus replication","authors":"Zhimin Li, Yi Chang Wu, Jian He, Kun Wu, Shaoping Weng, Changjun Guo, Jianguo He","doi":"10.1016/j.cirep.2025.200196","DOIUrl":"https://doi.org/10.1016/j.cirep.2025.200196","url":null,"abstract":"• Mandarin fish TNKS1-like evokes Wnt/β-catenin signal pathway. • Wnt/β-catenin signal pathway promotes ISKNV replication. • TNKS1-like positively regulates ISKNV replication. Tankyrase (TNKS), a poly (ADP-ribose) polymerase (PARP) protein, mediates the PARylation of substrates, ultimately guiding them towards proteasome-dependent degradation or altering their localization. Recent studies suggest that TNKS may play a crucial role in both innate immunity and viral infection, yet its function in teleost fish remains unexplored. This study aimed to investigate the roles of mandarin fish ( Siniperca chuatsi ) TNKS1-like ( sc TNKS1-like) in the Wnt/β-catenin signaling pathway and its impact on infectious spleen and kidney necrosis virus (ISKNV) infection. Bioinformation analysis results showed that sc TNKS1-like contained ANK, SAM, and PARP domains, with its sequence highly conserved across different species. Expression pattern analysis showed that sc TNKS1-like mRNA is ubiquitously distributed in all tissues and was significantly upregulated in response to immune stimuli and ISKNV infection. Dual-luciferase reporter assays indicated that sc TNKS1-like could evoke and positively regulate the Wnt/β-catenin signaling pathway. Protein degradation assays and co–immunoprecipitation experiment further showed that sc TNKS1-like interacted with and regulated axis inhibitory protein 1 to activate the Wnt/β-catenin signaling pathway. Moreover, it was found that sc β-catenin activates the Wnt/β-catenin signaling pathway, which in turn enhances ISKNV replication. Furthermore, sc TNKS1-like was observed to significantly upregulate ISKNV replication. These findings demonstrated the role of sc TNKS1-like in teleost fish and contribute to our understanding of its function in virus infection.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"8 1","pages":"200196-200196"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a cell line from the gill of turbot (Scophthalmus maximus) and its application in the study of Edwardsiella piscida infection","authors":"Yuping Liu, Yanmin Wang, Yiping Liu, Xuefeng Ge, Chao Li, Ting Xue","doi":"10.1016/j.cirep.2024.200192","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200192","url":null,"abstract":"• A new cell line from the gill of Scophthalmus maximus was established. • The fundamental characteristics of the cell line were systematically analyzed. • The cell line is an ideal model for studying the interaction of host and E. piscicida . In this study, we established and characterized a continuous cell line derived from the gill tissue of turbot ( Scophthalmus maximus ) and assessed its applicability in studying the gill's function as a mucosal immune barrier. This cell line, designated SMG, has been successfully subcultured for up to 70 passages. The SMG cells exhibited robust growth in DMEM medium supplemented with 20 % FBS at 24 °C. Karyotype analysis revealed that the modal chromosome number of SMG cells was 44. Amplification of the 18S rRNA confirmed the turbot origin of the SMG cells. The cell line was transfected with a pEGFP-N1 plasmid and FAM labeled siRNA, resulting in high transfection efficiency and successful expression of the transfection reporter gene and FAM fluorescence signals 48 h post-transfection. Edwardsiella piscicida replicated in SMG cells at low concentrations without altering the morphology of the cells in the early stages of infection. These findings indicate the potential utilization of SMG cells in studying gene function and antimicrobial responses. This research provides a foundation for future studies on the immune barrier of gill mucosa in marine species.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"8 1","pages":"200192-200192"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological process of T cells and IgM+ B cells in head kidney and peripheral blood in early vertebrate Nile tilapia","authors":"Yuying Zheng, Qian Zhang, Yating Zhu, Jiansong Zhang, Ming Geng, Yishan Lu, Kang Li, Xiumei Wei","doi":"10.1016/j.cirep.2024.200189","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200189","url":null,"abstract":"• T and B cells in HKL and PBL involve in anti-bacterial response of tilapia. • T and B cells in HKL and PBL involve in the secondary immune response of tilapia. • T cells in HKL and PBL perform effector function in tilapia. T and B lymphocytes are the primary effectors of the immune system's specific anti-infective functions, abundantly concentrated in immune-related tissues and organs. The two cell types work in different mechanisms to combat pathogens through precision guidance and jointly accomplish the immune response. Previous studies have elucidated the critical role of splenic T cells in antimicrobial infections in Nile tilapia ( Oreochromis niloticus ). On this basis, this study aimed to further reveal the distribution and effector functions of T and B lymphocytes in the head kidney and peripheral blood of teleosts. Here, the distribution and effector functions of T cells and IgM + B cells were examined using a model of Edwardsiella piscicida infection in Nile tilapia. The results verified that T and B cells were distributed in the peripheral blood and spleen of Nile tilapia, with primary and secondary infection induced remarkable increases of T cells and IgM + B cells in the head kidney and peripheral blood. Notably, T cells and IgM + B cells proliferated rapidly in secondary infection than initial immune response, implying that immune memory as a hallmark feature of adaptive immunity was already present in early vertebrates. More importantly, the inhibition of T cells during bacterial infection impaired the expression of perforin A and granzyme B . Thus, this study indicated the distribution and effector functions of T cells and IgM + B cells during specific immunity in Nile tilapia, and provided theoretical support for understanding the evolutionary basis of the adaptive immune system.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"8 1","pages":"200189-200189"},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characteristics of IL-8 and its potential receptor CXCR1/2 in Siberian hybrid sturgeon","authors":"Shiyong Yang, Rong Wei, Xinrui Lyu, Yujie Lv, Yang Xiao, Jiayun Wu, Xiaogang Du, Yunkun Li","doi":"10.1016/j.cirep.2024.200188","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200188","url":null,"abstract":"• Four CXCR1 and CXCR2 were cloned and identified from Siberian hybrid sturgeon, named as CXCR1-1, CXCR1-2, CXCR2-1 and CXCR2-2. • An unique IL-8 was cloned and identified from Siberian hybrid sturgeon, which belongs to fish CXCL8-L2 subfamily. • IL-8 and CXCR1/2 genes wildly express in different tissues and respond to Aeromonas hydrophila and Staphylococcus aureus challenges. • Recombinant IL-8 exhibits concentration-dependent chemotactic activity for blood granulocytes. IL-8, also known as CXCL8, was the first chemokine identified in mammals, playing a crucial role in regulating inflammatory responses and immune cell chemotaxis. In fish, IL-8 exhibits a more diverse and complex function that makes it being perplexing. This research focused on cloning the coding sequences (CDS) of IL-8 gene and its potential receptor genes (named CXCR1-1, CXCR1-2, CXCR2-1 , and CXCR2-2 ) from Siberian hybrid sturgeon ( Acipenser baerii ♀× Acipenser schrenckii ♂). Their molecular features, tissue distribution and immune function were analyzed. The CDS length of IL-8 and the receptor genes was 288 bp, 1,135 bp, 1,135 bp, 1,061 bp, and 1,078 bp, respectively. Multiple sequence alignment and phylogenetic analysis showed the relative conservation of their amino acids compared to other fish species. IL-8 and the receptors were broadly expressed in various tissues. Upon infection with Aeromonas hydrophila ( A. hydrophila ) or Staphylococcus aureus ( S. aureus ), the expression levels of IL-8 and CXCR2-1/2 were increased in the spleen and head kidney, with a strong expression correlation observed between IL-8 and CXCR2-1/CXCR2-2 in the spleen. Furthermore, recombinant IL-8 demonstrated concentration-dependent chemotactic activity for granulocytes. This study offers valuable insights into the potential signaling pathways and functions of IL-8, thereby contributing to the future in-depth investigation of IL-8-mediated signaling activation in cartilaginous fishes.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"8 1","pages":"200188-200188"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A subunit vaccine of largemouth bass (Micropterus salmoides) with baculovirus-expressed largemouth bass ranavirus (LMBRaV) major capsid protein induces protective immunity against LMBRaV infection","authors":"Yiqun Li, Yanlin Dai, Mingyang Xue, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Chen Xu, Zhenyu Huang, Yangyang Xing, Jinyu Shen, Yuding Fan","doi":"10.1016/j.cirep.2024.200179","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200179","url":null,"abstract":"• The MCP of LMBRaV was successfully expressed through Bac-to-Bac baculovirus expression system. • AcMNPV-MCP enhanced the length and weight of largemouth bass. • AcMNPV-MCP can significantly reduce the LMBRaV viral load in the target tissues. • AcMNPV-MCP activated the anti-viral immune responses in largemouth bass. Largemouth Bass Ranavirus (LMBRaV) is associated with large mortalities of farmed Largemouth Bass ( Micropterus salmoides ), which has caused huge economic loss to its aquaculture in China. The major capsid protein (MCP), an effective antigen to induce a specific immune response in iridovirus, has been widely used as immunogen in vaccination experiments. In this study, we constructed the recombinant baculovirus autographa californica multiple nucleopolyhedrovirus-MCP (AcMNPV-MCP) through Bac-to-Bac baculovirus expression system and expressed MCP as a subunit vaccine in insect cells. Recombinant baculovirus was verified using PCR and the virus titer was calculated as 50 % tissue culture infectious dose (TCID 50 /mL) using the Reed-Muench method. After confirmed by western blot, the recombinant MCP was purified from baculovirus-infected cell lysates and injected intraperitoneally (12 µg/fish) into M. salmoides . Liver, spleen and head kidney were collected at indicated times to analyze the expression of anti-viral genes. The result showed that the mRNA expression levels of immune-related genes like interferon related factors and interleukins were significantly up-regulated in AcMNPV-MCP immunized group. Meanwhile, the number of leukocytes in immunized group were significantly higher than that in control group. After immunized for 35 days, M. salmoides were challenged with LMBRaV injection. After infection with LMBRaV, Non-vaccinated groups exhibited a mortality of 100 %, while the survival rate of vaccinated group was up to 63.3 %. qPCR found that the virus load of vaccinated group was significantly lower than that of Non-vaccinated groups. This finding will potentially allow for the development of more target subunit vaccine development against largemouth bass ranavirus.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"7 1","pages":"200179-200179"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemopexin regulates acute-phase inflammatory response against pathogenetic infections in black rockfish (Sebastes schlegelii)","authors":"Chengbin Gao, Peng Sun, Xin Cai, Mengyu Tian, Chao Li","doi":"10.1016/j.cirep.2024.200159","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200159","url":null,"abstract":"","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"15 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141709559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the connection between pathogen water eDNA and disease progression in zebrafish infected with Vibrio anguillarum","authors":"Cyril Hénard, Manuel Blonç, Hanxi Li, Yajiao Duan, Louise von Gersdorff Jørgensen","doi":"10.1016/j.cirep.2024.200160","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200160","url":null,"abstract":"","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"71 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141714765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immune system of marine invertebrates: Earliest adaptation of animals","authors":"R. B. Rathinam, Arpit Acharya, A. J. Robina, Husne Banu, G. Tripathi","doi":"10.1016/j.cirep.2024.200163","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200163","url":null,"abstract":"","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta‐analysis of the effects of black soldier fly meal on fish immune response and antioxidant capacity","authors":"Yizhi Xiao, Lezhao Zhu, Renhao Liang, Jiequan Su, Junqi Yang, Xinyi Cao, Yao Lu, Yiyi Yu, Junjie Hu","doi":"10.1016/j.cirep.2024.200162","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200162","url":null,"abstract":"","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"16 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141709494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese inhibits SVCV infection by promoting the expression of STING and the subsequent activation of IFN","authors":"Ke-Jia Han, Bao-Jie Cui, Xiao Xu, Chu-Jing Zhou, Can Zhang, Zhuo-Cong Li, Xueli Wang, Xiaoli Yang, Dandan Chen, Jing-Shuai Li, Long-Feng Lu, Shun Li","doi":"10.1016/j.cirep.2024.200158","DOIUrl":"https://doi.org/10.1016/j.cirep.2024.200158","url":null,"abstract":"Manganese (Mn) is necessary for various biological processes, yet its function in innate immunity has been less studied, especially in fish. Stimulator of interferon (IFN) genes (STING) is an essential factor for IFN induction in virus infection. Our study demonstrated that Mn2+ increases the ability of zebrafish to resist spring viraemia of carp virus (SVCV) infection by enhancing the expression of STING. Further mechanistic studies indicated that Mn2+ promotes the expression of STING by increasing its ubiquitination. Furthermore, Mn2+treatment promoted STING-mediated activation of IFN and reversed the decrease in STING protein induced by SVCV. Collectively, our findings showed the vital involvement and necessity of Mn2+ in protecting fish against SVCV infection.","PeriodicalId":516937,"journal":{"name":"Comparative Immunology Reports","volume":"7 1","pages":"200158-200158"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}