Zhonghua shao shang yu chuang mian xiu fu za zhi最新文献

{"title":"[Influence and mechanism of PBNP combined with mouse ADSCs on full-thickness skin defect wounds in diabetic mice].","authors":"X W Zhang, S Y Xu, Y J Han, X M Li, G Xu","doi":"10.3760/cma.j.cn501225-20250106-00009","DOIUrl":"10.3760/cma.j.cn501225-20250106-00009","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the influence and mechanism of Prussian blue nanoparticles (PBNPs) combined with mouse adipose-derived mesenchymal stem cells (ADSCs) on full-thickness skin defect wounds in diabetic mice. <b>Methods:</b> This study was an experimental study using group design and repeated-measures design. PBNPs were prepared by hydrothermal synthesis, and their morphology was characterized by transmission electron microscopy. ADSCs were isolated from five male 6-8-week-old Institute of Cancer Research (ICR) mice, USA via collagenase digestion. The cells were divided into control group cultured under routine conditions, high-glucose group cultured with glucose in a final molarity of 30.0 mmol/L, and low-PBNP group and high-PBNP group pretreated with 10 or 20 μg/mL PBNP for 12 h, respectively, followed by the same treatment as in high-glucose group. After 24 h of culture, cell viability was assessed using the cell counting kit-8, the proportion of senescent cells in the cells was detected by β-galactosidase staining, and the protein expression levels of senescence-associated proteins p16 and p21 were determined by Western blotting. Twenty-four male 6-8-week-old ICR mice were used to establish the diabetic model. A full-thickness skin defect wound was then created on the back of each mouse. The injured mice were divided into four groups (with 6 mice in each group) according to the random number table method, including control group with wounds treated with normal saline, ADSC group with wounds treated with normal saline containing 5×10<sup>6</sup> ADSC (the same number of cells used below), low-PBNP group and high-PBNP group with wounds treated with normal saline containing ADSC pretreated with 10 and 20 μg/mL PBNP for 12 h, respectively. Wound healing was observed at post-injury day (PID) 0 (immediately), 3, 7, 10, and 14. The percentage of remaining wound area was calculated at PID 3, 7, 10, and 14. At PID 7, the protein expression levels of the cell proliferation markers Ki67 and vascular endothelial growth factor (VEGF) in wound tissue were detected by immunofluorescence method. At PID 14, the expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10 in wound tissue were determined by enzyme-linked immunosorbent assay. <b>Results:</b> PBNPs exhibited a dispersed regular cubic structure. After 24 h of culture, compared with those in control group, cells in high-glucose group showed a significantly lower cell viability (<i>P</i><0.05), a significantly higher proportion of senescent cells (<i>P</i><0.05), and significantly higher protein expression levels of p16 and p21 (<i>P</i><0.05). Compared with those in high-glucose group, cells in low-PBNP and high-PBNP groups showed significantly higher cell viability (<i>P</i><0.05), significantly lower proportions of senescent cells (<i>P</i><0.05), and significantly lower protein expression levels of p16 and p21 (<i>P</i><0.05). From PID 0 to 14,","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 7","pages":"659-668"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13416971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evolution of DFU treatment strategies: from simple coverage with traditional dressings to active modulation with smart materials].","authors":"X L Chen","doi":"10.3760/cma.j.cn501225-20260409-00146","DOIUrl":"10.3760/cma.j.cn501225-20260409-00146","url":null,"abstract":"<p><p>Diabetic foot ulcers (DFUs) fall into a vicious cycle of difficulty healing due to hyperglycemia-induced persistent inflammation, accumulation of reactive oxygen species (ROS), tissue hypoxia, and susceptibility to infection. This paper reviews the pathological microenvironmental characteristics of DFUs, the design strategies of smart materials, and their integration with artificial intelligence (AI). Given the complex pathological microenvironment of DFUs, traditional dressings that provide simple coverage are no longer adequate, whereas responsive smart materials that are capable of dynamically sensing and actively modulating the wound microenvironment have demonstrated great potential. Furthermore, the paper highlights four key design strategies for responsive smart materials: using glucose-responsive smart materials to improve local hyperglycemia at the wound site; using ROS-scavenging smart materials to eliminate ROS and restore redox homeostasis; applying oxygen-generating materials to relieve hypoxia and to promote angiogenesis; and utilizing smart antibacterial materials to combat microbial biofilms and achieve potent bactericidal effects. Currently, the development trend of smart materials has shifted from single functionality towards integrated multifunctional synergistic systems. The paper further discusses the potential of integrating AI into material design, preparation optimization, and wound monitoring and treatment decision-making. Although most smart materials are still at the experimental stage and face challenges related to cost and manufacturing processes, next-generation smart materials are expected to achieve dynamic monitoring and autonomous treatment through interdisciplinary innovation, thereby fundamentally improving DFU healing rates and reducing the risk of amputation in patients.</p>","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 7","pages":"603-610"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13416972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical efficacy of pedicled double-perforator flap of the inferior epigastric artery in reconstructing the soft tissue defects following resection of cutaneous malignant tumors].","authors":"S P Zhou, D Zhang, H L Wei, S M Li, J J Chen, S Li, P C Shi, X F Xing, C N Chang, Y G Shi, L W Zheng, H P Wang, Y N Gai","doi":"10.3760/cma.j.cn501225-20250227-00096","DOIUrl":"10.3760/cma.j.cn501225-20250227-00096","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the clinical efficacy of pedicled double-perforator flap of the inferior epigastric artery in reconstructing the soft tissue defects following resection of cutaneous malignant tumors adjacent to major vessels and/or nerves in the groin or thigh. &lt;b&gt;Methods:&lt;/b&gt; This study was a retrospective case series study. From June 2016 to June 2023, 12 patients (8 males and 4 females, aged 58 to 76 years) with cutaneous malignant tumors in the groin or thigh who met the inclusion criteria were admitted to the Department of Burn Plastic Surgery and Wound Healing of the 988&lt;sup&gt;th&lt;/sup&gt; Hospital of the Joint Logistics Support Force of PLA. In all patients, the depth of tumor invasion extended close to the major vessels and/or nerves. Radical extended resection of the tumors was performed, resulting in postoperative soft tissue defects ranging from 16 cm×13 cm to 37 cm×17 cm in size. A pedicled double-perforator flap of the inferior epigastric artery based on the paraumbilical and thoracic paraumbilical perforators was designed and harvested, with flap dimensions ranging from 15 cm×14 cm to 38 cm×18 cm. The flaps were transferred to the recipient site wound through a subcutaneous tunnel and sutured in place. Primary closure of the donor site wound was achieved in 8 patients; in the remaining 4 patients, the donor site wound was first approximated with sutures to reduce its size, then covered with negative-pressure sealing drainage dressing, with secondary closure performed at a later stage. Postoperative observations included flap blood supply, flap survival, occurrence of vascular crisis, and wound healing of donor and recipient sites. Operative duration and length of hospital stay of patients were recorded. During follow-up after surgery, tumor recurrence, flap contour, and donor site scar were observed, and whether abdominal wall function was affected by the scar was assessed. &lt;b&gt;Results:&lt;/b&gt; All 12 patients achieved good flap blood supply and uneventful survival, with no vascular crisis. Wounds in both donor and recipient sites healed successfully. Operative duration of patients ranged from 2.0 to 3.0 hours, and length of hospital stay ranged from 15 to 24 days, with a mean of 18.6 days. During a follow-up period of 1 to 6 years after surgery, no local tumor recurrence was observed. Some flaps appeared slightly bulky, but their elasticity and texture showed no significant differences compared with the surrounding tissue. The donor site only exhibited a linear scar, with no significant impact on abdominal wall function. &lt;b&gt;Conclusions:&lt;/b&gt; For soft tissue defects following resection of cutaneous malignant tumors adjacent to major vessels and/or nerves in the groin or thigh, the pedicled double-perforator flap of the inferior epigastric artery is a viable reconstructive option. This flap provides a large surface area for effective reconstruction while minimizing the impact on aesthetics and function of the donor site.&lt;/","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 7","pages":"641-648"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13416982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of factors influencing tension blister formation on the skin at the dressing edges in patients undergoing negative pressure wound therapy].","authors":"H H Guo, T Ning, M M Ma, S W Mei, X J Ruan","doi":"10.3760/cma.j.cn501225-20240911-00334","DOIUrl":"10.3760/cma.j.cn501225-20240911-00334","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the factors that influence the formation of tension blisters on the skin at the dressing edges in patients undergoing negative pressure wound therapy (NPWT). &lt;b&gt;Methods:&lt;/b&gt; This study was a retrospective case-control study. From September 2020 to August 2023, 187 patients undergoing NPWT who met the inclusion criteria were admitted to the Department of Orthopedics of Fuyang People's Hospital Affiliated to Anhui Medical University, including 112 males and 75 females, aged (52±17) years. Based on the presence or absence of tension blisters on the skin at the edge of NPWT filling dressings (hereinafter referred to as dressings) after 3 days of NPWT, the patients were divided into no blister group (97 cases) and blister group (90 cases). The clinical characteristics (gender, age, systolic blood pressure on admission, diastolic blood pressure on admission, body mass index, wound area, duration of wound debridement, wound infection status, concomitant fractures, wound irrigation, dressing application site, and dressing management method) as well as biochemical indicators on day 1 after admission were compared between the two groups. The independent influencing factors for tension blister formation at the dressing edges in 187 patients after 3 days of NPWT were screened. &lt;b&gt;Results:&lt;/b&gt; There were statistically significant differences between the two groups of patients in terms of age, dressing application site, and dressing management method (&lt;i&gt;t&lt;/i&gt;=2.057, with &lt;i&gt;χ&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; values of 5.589 and 27.686, respectively, &lt;i&gt;P&lt;/i&gt;&lt;0.05); there were no statistically significant differences in the other clinical characteristics or biochemical indicators on day 1 after admission (&lt;i&gt;P&lt;/i&gt;&gt;0.05). Univariate logistic regression analysis results showed that age, dressings treated with petrolatum jelly gauze and dressing trimming were protective factors against the formation of tension blisters on the skin at the dressing edges in patients after 3 days of NPWT (with &lt;i&gt;OR&lt;/i&gt;s of 0.983, 0.130, and 0.381, respectively, 95% &lt;i&gt;CIs&lt;/i&gt; of 0.966-0.999, 0.058-0.287, and 0.178-0.816, respectively, &lt;i&gt;P&lt;&lt;/i&gt;0.05); trunk was the risk factor facilitating the formation of tension blisters on the skin at the dressing edges in patients after 3 days of NPWT (&lt;i&gt;OR&lt;/i&gt;=2.028, with 95% &lt;i&gt;CI&lt;/i&gt; of 1.125-3.655, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Multivariate logistic regression analysis results showed that trunk was the independent risk factor facilitating the formation of tension blisters on the skin at the dressing edges in patients after 3 days of NPWT (&lt;i&gt;OR&lt;/i&gt;=2.566, with 95% &lt;i&gt;CI&lt;/i&gt; of 1.306-5.043, &lt;i&gt;P&lt;/i&gt;&lt;0.05); dressings treated with petrolatum jelly gauze and dressing trimming were independent protective factors against the formation of tension blisters on the skin at the dressing edges in patients after 3 days of NPWT (with &lt;i&gt;OR&lt;/i&gt;s of 0.111 and 0.363, respectively, 95% &lt;i&gt;CIs&lt;/i&gt; of 0.048-0.257 and 0.165-0.797, respectively, &lt;i&gt;P&lt;/i&gt;","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"572-578"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Culture and identification of human induced pluripotent stem cell-derived skin organoids].","authors":"J Z Xia, Z X Wang, D Sun, Z D Zhang, Y N Ding, B Chen, M X Xia, Z R Liu","doi":"10.3760/cma.j.cn501225-20260205-00074","DOIUrl":"10.3760/cma.j.cn501225-20260205-00074","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To establish and identify a culture system for skin organoids based on human induced pluripotent stem cells (hiPSCs). &lt;b&gt;Methods:&lt;/b&gt; This study was a basic research with a self-controlled pre-post design. Human primary skin fibroblasts (Fbs) were obtained from discarded hair follicle tissue of a 45-year-old male patient undergoing hair transplant surgery at Tongji Medical College of Huazhong University of Science and Technology. Primary skin Fbs were induced into hiPSCs, which were then cultured to form three-dimensional aggregates. Then the induced differentiation was performed as followings. On differentiation day 0 (the day of differentiation initiation), the three-dimensional aggregates were transferred to ultra-low attachment culture plate and cultured in E6 medium supplemented with Matrigel, transforming growth factor-β type Ⅰ receptor inhibitor of SB431542, basic fibroblast growth factor (bFGF), and bone morphogenetic protein-4 to induce non-neural ectoderm formation. On differentiation day 3, one-quarter volume of E6 medium supplemented with bone morphogenetic protein signaling pathway inhibitor of LDN193189 and bFGF was added to the original medium to continue culturing and to induce the formation of cranial neural crest cells. On differentiation day 6, culture continued with approximately 3/5 volume of plain E6 medium added to the original medium. Half-medium changes were performed on differentiation days 8 and 10 (once each), followed by continued culture. On differentiation day 12, cell clumps were seeded into new ultra-low attachment culture plate to continue culturing, and epidermal self-assembly was induced using organoid maturation medium supplemented with Matrigel. On differentiation day 15, a half-medium change was performed, and culture was continued. On differentiation day 18, the original medium was replaced with organoid maturation medium supplemented with α-melanocyte-stimulating hormone to continue culturing. Starting from differentiation day 21, half-medium changes were performed every 3 days, and culture continued. After differentiation initiation, the stage-specific morphological characteristics of hiPSC differentiation into skin organoids were observed daily. Immunofluorescence staining was performed to assess skin organoids on different days of differentiation: on day 12 for the presence of mesenchymal cells, on day 20 for epidermal terminal differentiation, on day 35 for mesenchymal and epithelial structure formation as well as hair follicle stem cell and basal layer keratinocyte related characteristics, on day 55 for dermal papilla formation, on day 75 for hair germ-like structure formation, and on day 90 for organoid proliferative activity. Fluorescence probe staining was performed on differentiation day 110 to detect hair follicle formation, as well as lipid deposition and sebaceous gland-like structure formation. &lt;b&gt;Results:&lt;/b&gt; On differentiation day 0, hiPSCs formed three-dimensional ag","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"542-551"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Influence of human skin organoid-derived extracellular vesicles composite hydrogels on wound healing of full-thickness skin defects in mice].","authors":"R Y Li, Q R Zhou, C R He, G C Wang, X Chen, J C Su","doi":"10.3760/cma.j.cn501225-20260107-00013","DOIUrl":"10.3760/cma.j.cn501225-20260107-00013","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the influence of human skin organoid-derived extracellular vesicles (SOEVs) composite hydrogels on wound healing of full-thickness skin defects in mice. &lt;b&gt;Methods:&lt;/b&gt; This study was an experimental study using a group design and a repeated-measures design. HaCaT cells, human skin fibroblasts, and human umbilical vein endothelial cells (HUVECs) were mixed in a ratio of 2∶1∶1 and cultured three-dimensionally in 6-well ultra-low attachment plates to generate skin organoids. The formation of skin organoids was observed on days 1, 3, 7, and 14 of culture. On day 7 of culture, the expressions of epidermal marker cytokeratin 14 (CK14), vascular marker CD31, and dermal marker vimentin in skin organoids were detected by immunofluorescence method. On day 7 of culture, SOEVs were isolated from the culture supernatant of skin organoids by sequential differential ultracentrifugation. The ultrastructure of SOEVs was observed using transmission electron microscopy, and the particle size of SOEVs was measured using a nanoparticle tracking analyzer. HaCaT cells and HUVECs were collected, and both cell types were divided into a SOEV group cultured with 30 μg/mL SOEV and a control group cultured under conventional condition. A scratch test was performed, and cell migration rate was calculated at 24 h after scratching (&lt;i&gt;n&lt;/i&gt;=6). Gelatin methacrylate anhydride (GelMA) hydrogel and GelMA hydrogel containing 30 μg/mL SOEV (i.e. SOEV composite hydrogel) were prepared. Eighteen 6-week-old male C57BL/6J mice were divided into a control group, a GelMA group, and a SOEV@GelMA group using a random number table method, with 6 mice in each group. A full-thickness skin defect wound was created on the back of each mouse. Immediately after injury (day 0), phosphate-buffered saline, GelMA hydrogel, and SOEV composite hydrogel were applied to the wounds of mice in control group, GelMA group, and SOEV@GelMA group, respectively. Wound healing was observed at post-injury day (PID) 0, 3, 7, 10, and 14, and the wound healing rate was calculated at PID 3, 7, 10, and 14. At PID 14, wound tissue was collected. Hematoxylin-eosin staining was performed to observe the degree of wound epithelial regeneration; Masson staining was performed to observe collagen fiber deposition, and the proportion of collagen fiber-positive area was calculated. &lt;b&gt;Results:&lt;/b&gt; After 1 to 14 days of culture, the skin organoids gradually matured, with the structures becoming increasingly compact and dense, and the spheroids exhibiting clear boundaries. On day 7 of culture, the skin organoids expressed CK14, CD31, and vimentin. SOEVs extracted from the culture supernatant of skin organoids on day 7 of culture exhibited a typical disc-shaped vesicular structure, with an average particle size of 70.1 nm. At 24 h post-scratching, the migration rates of HaCaT cells and HUVECs in SOEV group were significantly higher than those in corresponding control group (with &lt;i&gt;t&lt;/i&gt; value","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"532-541"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of the characteristics and changing trends of burn disease burden in the Chinese population based on the GBD 2021 database].","authors":"J Y Liang, M M Xi, N Tang, W Tian, J Zhang, J Y Peng, Y Z Xin, Rebecca Ivers, P P Ye, M Y Tian","doi":"10.3760/cma.j.cn501225-20251208-00512","DOIUrl":"10.3760/cma.j.cn501225-20251208-00512","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To analyze the characteristics and changing trends of burn disease burden in the Chinese population based on the Global Burden of Disease (GBD) 2021 database. &lt;b&gt;Methods:&lt;/b&gt; This study was a secondary study based on public databases. Data related to burns caused by \"fire, heat, and hot substances\" in 22 provinces, 5 autonomous regions, 4 municipalities, Hong Kong Special Administrative Region, and Macao Special Administrative Region of China except for the Taiwan region of China from 1990 to 2021 were extracted from the GBD 2021 database. Stratified analyses were conducted by gender, age bracket, and provincial-level administrative region. Disability-adjusted life year (DALY) rate, years of life lost (YLL) rate, years lived with disability (YLD) rate, and their age-standardized rates were used to evaluate the burn disease burden. The changing trends of burn disease burden among populations across different genders, age brackets and provincial-level administrative regions in China from 1990 to 2021 were compared. The burn disease burden among populations across different genders, age brackets and provincial-level administrative regions in China in 2021 was evaluated. &lt;b&gt;Results:&lt;/b&gt; From 1990 to 2021, the burn disease burden in the Chinese population decreased overall. The overall age-standardized DALY rate (95% &lt;i&gt;UI&lt;/i&gt;) of burns in the Chinese population decreased from 135.6 (109.8-158.4)×10&lt;sup&gt;-5&lt;/sup&gt; in 1990 to 33.6 (27.0-41.8)×10&lt;sup&gt;-5&lt;/sup&gt; in 2021, with a decline of 75.2%. The age-standardized YLL rate and age-standardized YLD rate of burns also decreased, with declines of 81.4% and 54.5%, respectively. Compared with that in 1990, the age-standardized DALY rates of burns in all provincial-level administrative regions decreased in 2021, but the magnitudes of decline varied. In 25 provincial-level administrative regions, the decline in age-standardized DALY rate of burns was more pronounced in women than in men, whereas the opposite pattern was observed in 8 provincial-level administrative regions. The burn DALY rate decreased relatively rapidly in young children aged 1 to &lt;2 years, while it decreased relatively slowly in the oldest-old population aged ≥80 years. In 2021, differences in burn disease burden were observed among populations across different genders, age brackets and provincial-level administrative regions in China. In all age brackets, burn DALY rates in men were higher than those in women. Among age brackets, the burn DALY rate (95% &lt;i&gt;UI&lt;/i&gt;) was the highest in the ≥80 years age bracket, at 147.3 (114.5-176.3)×10&lt;sup&gt;-5&lt;/sup&gt;. In 2021, the top 3 provincial-level administrative regions with relatively high age-standardized DALY rates of burns, in descending order, were Yunnan Province, Guizhou Province, and Shanghai, with values of 45.4 (37.2-58.7)×10&lt;sup&gt;-5&lt;/sup&gt;, 43.8 (34.3-55.4)×10&lt;sup&gt;-5&lt;/sup&gt;, and 43.3 (29.6-62.2)×10&lt;sup&gt;-5&lt;/sup&gt;, respectively. &lt;b&gt;Conclusions:&lt;/b&gt; From 1990 to 2021, the burn di","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"589-596"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and validation of a predictive model for the risk of ARDS in severely burned patients].","authors":"Y Yao, W Liu, S S Wang, T Z Hua, G L Zhang, H R Jia, S J Ma, Z A Shen","doi":"10.3760/cma.j.cn501225-20260204-00070","DOIUrl":"10.3760/cma.j.cn501225-20260204-00070","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To construct and validate a nomogram model for predicting the risk of acute respiratory distress syndrome (ARDS) in severely burned patients. &lt;b&gt;Methods:&lt;/b&gt; This study was a retrospective cohort study. From January 2015 to August 2025, 372 severely burned patients who met the inclusion criteria were admitted to the Fourth Medical Center of Chinese PLA General Hospital, including 279 males and 93 females, aged 18 to 78 years. According to whether ARDS occurred within 7 days post-injury, patients were divided into ARDS group and non-ARDS group. Stratified random sampling was used to allocate patients into training set and validation set in a ratio of 7∶3. In training set, there were 35 patients in ARDS group and 227 patients in non-ARDS group; in validation set, there were 14 patients in ARDS group and 96 patients in non-ARDS group. General clinical data of the patients were collected, including total burn area, modified Baux score, burn index, partial-thickness burn area, full-thickness burn area, severity of inhalation injury, whether mechanical ventilation or high-flow oxygen therapy performed upon admission, number of days after tracheostomy, length of hospital stay, duration in critical illness, and mortality rate, as well as data related to post-burn ARDS development within 24 hours of admission, including white blood cell count, neutrophil count, thrombin time, and levels of hemoglobin, creatinine, chloride, procalcitonin, albumin, and fibrinogen (FIB). Variables with statistically significant differences between two groups in training set were screened, and characteristic variables were further selected using least absolute shrinkage and selection operator (LASSO) regression combined with a weight adjustment strategy. Multivariate logistic regression analysis was performed to identify independent predictors, based on which a nomogram model was constructed. The performance of the model was validated in both training set and validation set. &lt;b&gt;Results:&lt;/b&gt; In the general clinical data of training set, patients in ARDS group had significantly larger total burn area and full-thickness burn area (&lt;i&gt;P&lt;/i&gt;&lt;0.05), significantly higher modified Baux score, burn index, proportion of patients receiving mechanical ventilation or high-flow oxygen therapy upon admission, and mortality rate (&lt;i&gt;P&lt;/i&gt;&lt;0.05), significantly more severe degree of inhalation injury (&lt;i&gt;P&lt;/i&gt;&lt;0.05), significantly greater number of days after tracheostomy, length of hospital stay, and duration in critical illness (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and significantly smaller partial-thickness burn area (&lt;i&gt;P&lt;/i&gt;&lt;0.05) than those in non-ARDS group. With the data related to post-burn ARDS development, patients in ARDS group had significantly higher white blood cell count, neutrophil count, and levels of hemoglobin, creatinine, chloride, and procalcitonin (&lt;i&gt;P&lt;/i&gt;&lt;0.05), significantly longer thrombin time (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and significantly lower levels of albumin and FIB (&lt;i","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"552-561"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Organoid-based strategies and challenges in tissue regeneration].","authors":"G C Wang, J C Su","doi":"10.3760/cma.j.cn501225-20260119-00033","DOIUrl":"10.3760/cma.j.cn501225-20260119-00033","url":null,"abstract":"<p><p>Tissue regenerative medicine aims to repair or replace tissue and organs that have lost function due to disease, trauma, or aging, and the emergence of organoid technology has provided a new avenue for this field. Organoids are self-organized structures formed under three-dimensional culture conditions. They can recapitulate the structure and function of native organs, demonstrating unique advantages in disease modeling, drug screening, personalized medicine, and regenerative application. This article systematically summarizes the core strategies of organoids in tissue regeneration, including <i>in vitro</i> modeling and drug screening, <i>in situ</i> transplantation and functional repair, biohybrid organ construction, and organoid chip platform. It further analyzes the major challenges such as insufficient technological maturity, limited functional fidelity, restricted large-scale production, and ethical regulations for clinical application. In addition, potential solutions are proposed to overcome current bottlenecks and challenges through technological integration, standardization and automation, and the promotion of clinical translation. Overall, organoids, as a key strategy in regenerative medicine, are reshaping the landscape of tissue regeneration research and offering new hope for future clinical therapies.</p>","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"514-521"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research advances on the application of skin appendage organoids in wound repair].","authors":"M M Wang, Y T He, Q L Xie, W Zhou, X Xiang, M X Lei","doi":"10.3760/cma.j.cn501225-20260228-00092","DOIUrl":"10.3760/cma.j.cn501225-20260228-00092","url":null,"abstract":"<p><p>Skin wounds are often accompanied by structural damage and functional loss of appendages such as hair follicles and sweat glands, which cannot be fully restored by conventional repair methods. The development of skin organoid technology provides a new avenue to overcome this bottleneck. This article reviews the developmental history of skin organoids and systematically elaborates on the strategies and applications of skin appendage organoids in promoting functional skin regeneration. Furthermore, it analyzes the critical role of vascular and neural integration in improving the quality of wound repair. Finally, the article summarizes the current challenges associated with technologies such as stem cell induction, three-dimensional bioprinting, microfluidics, organ-on-a-chip, and air-liquid interface culture, and provides perspectives on the clinical translation of skin appendage organoids.</p>","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"42 6","pages":"597-602"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}