Problems of Particularly Dangerous Infections最新文献

{"title":"Population and Species Composition of Rodents and Insectivores in Settlements of the West Kazakhstan Region Located in the Plague Enzootic Territory","authors":"V. A. Tanitovsky, A. A. Gabbasov, V. V. Surov, V. G. Meka-Mechenko, Z. Z. Sayakova, N. S. Maikanov","doi":"10.21055/0370-1069-2023-4-135-140","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-135-140","url":null,"abstract":"The aim of the study was to analyze the abundance, species composition, dissemination of mouse-like rodents and other small mammals in the settlements of the West Kazakhstan Region over the past 6 years and the factors contributing to the change in the numbers in the long-term aspect.Materials and methods. We utilized the data obtained by employees of the Uralsk Plague Control Station, affiliated branch of the Republican State Enterprise “National Scientific Center of Particularly Dangerous Infections named after Masgut Aikimbaev” during a scheduled epizootiological survey of plague foci located in the West Kazakhstan Region.Results and discussion. The average annual number of rodents and insectivores in the settlements on the plague-enzootic territory of the West Kazakhstan Region was 5.1 in spring season, 8.1 in autumn, with 30.0 % occupancy of the facilities. In outbuildings, the number of rodents was three times higher than in residential buildings and amounted to 11.5 (residential buildings – 3.7). The general species composition of small mammals caught in the settlements was represented by seven species, among which the house mouse was a predominant one (97.5 %). The second place was occupied by the small shrew (1.7 %). There is a downward trend in the number of rodents in the steppe foci of plague (over 18 years – an average of 22.0 %). A slight increase in the number of animals by 2.0 % has been registered in the sandy focus.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"90 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of SARS‑CoV‑2 S Gene Mutations Using PCR during Seasons of Increased Incidence of Coronavirus Infection in the Chuvash Republic","authors":"N. P. Prishchepa, N. Y. Dobrovol’skaya, V. I. Nikiforova, T. S. Tarasova, E. V. Preobrazhenskaya","doi":"10.21055/0370-1069-2023-4-156-159","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-156-159","url":null,"abstract":"Mutations in the SARS‑CoV‑2 genome make it possible to effectively escape defense mechanisms of the host, which explains the spread of infection among vaccinated or previously affected by the virus individuals.The aim of the study was to investigate the dynamics of mutations in the SARS‑CoV‑2 virus genome during the rise of the seasonal incidence in the Chuvash Republic.Materials and methods. Under conditions of the clinical diagnostic laboratory of the Federal Center for Traumatology, Orthopedics and Endoprosthetics of the Ministry of Health of Russia (Cheboksary), samples, containing SARS‑CoV‑2 RNA, taken in January-February and July-October, 2022 were tested using reverse transcription PCR. The “MBS-Test SARS‑CoV‑2 RNA” (Technical Specifications 21.20.23-068-26329720-2021, Russia) and “AmpliTest SARS‑CoV‑2 VOC v.3” (Series V017, Certificate of Registration No. 2022/16307, Russia) were utilized in compliance with the manufacturer’s instructions.Results and discussion. Variations in the sets of SARS‑CoV‑2 S gene mutations have been revealed in the studied samples obtained during different periods of the spread of SARS‑CoV‑2 coronavirus. Timely detection of various mutations in the virus genome at the beginning of the epidemiological season and the alleged rise in the incidence of coronavirus infection is valuable information for forecasting the rate of virus transmission. It can also be used to create vaccines (taking into account changes in the virus genome) and to choose the adequate tactics for treating coronavirus infection.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Marker Detection of Natural-Focal Infectious Disease Pathogens in Samples of Ixodidae Ticks, Collected on the Territory of the Republic of Guinea","authors":"E. V. Naidenova, K. S. Zakharov, M. Kartashov, D. A. Agafonov, A. M. Senichkina, A. D. Katyshev, M. Diallo, M. B. Bah, S. Boumbaly, V. V. Kutyrev","doi":"10.21055/0370-1069-2023-4-115-124","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-115-124","url":null,"abstract":"The circulation of a rather wide range of pathogens of natural-focal infectious diseases transmitted by ticks was detected in West Africa at different points of time: Borrelia, Rickettsia, Coxiella, Crimean-Congo hemorrhagic fever (CCHF), Bhanja, and bluetongue viruses, etc. Current epidemiological and epizootiological situation on natural-focal infectious diseases on the territory of the Republic of Guinea is not entirely clarified.The aim of this work was to identify genetic markers (RNA/DNA) of natural-focal infectious disease agents in samples of Ixodidae ticks collected in the Republic of Guinea, and to determine the spectrum of pathogens circulating in various landscape-geographical zones of the country.Materials and methods. To conduct research on the territory of all landscape-geographical zones of the Republic of Guinea, 4695 specimens of Ixodidae ticks of 11 species were collected. Taking into account the species appurtenance, gender, phase of development, as well as the site of collection, a panel of 1645 samples was compiled. Genetic markers of Crimean-Congo hemorrhagic fever and tick-borne encephalitis viruses, as well as Borrelia burgdorferi s.l., Ehrlichia chaffeensis, Ehrlichia muris, Anaplasma phagocytophilum, Coxiella burnetii, Rickettsia of the tick-borne spotted fever (TBSF) group, and Francisella tularensis were detected using polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR).Results and discussion. The following markers of natural-focal disease agents were found in the Ixodidae tick suspensions: DNA of Rickettsia of the TBSF group (25.6 % of all samples studied), DNA of C. burnetii (6.2 %), cDNA of B. burgdorferi s.l. (9.1 %), and RNA of the CCHF virus (2.5 %). The listed spectrum of pathogens has been registered in all landscape-geographical zones of Guinea. Genetic markers of tularemia, anaplasmosis, ehrlichiosis and tick-borne encephalitis pathogens have not been identified in this study. The results obtained made it possible to clarify the probable spectrum of tick-borne diseases in the territory of the Republic of Guinea, determined the need for further study of the circulation of natural-focal infectious disease agents in West Africa and conducting regular epizootiological monitoring.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"37 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibility of Using Disposable Polymeric Containers in the Production Technology of Live Plague Vaccine","authors":"D. A. Sharov, I. V. Darmov, I. V. Kosenkov, A. A. Leshchenko, S. V. Bagin, D. A. Mokhov, A. G. Lazykin, E. A. Kovalenko","doi":"10.21055/0370-1069-2023-4-149-155","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-149-155","url":null,"abstract":"The aim of the work was to assess the possibility of using disposable polymeric containers in the production technology of the live plague vaccine.Materials and methods. We deployed the vaccine strain Yersinia pestis ЕV NIIEG for the work. A standard disposable 10 L Flexboy type polymeric container manufactured by Sartorius Stedim Biotech, equipped with Sartopore 2 filter capsules, was used for submerged cultivation of plague microbe inoculum. This method was compared to the regulated technology for obtaining seed cultures using glass bottles with a volume of 20 liters. The control of the produced seed cultures of the vaccine strain EV was performed in accordance with FS.3.3.1.0022.15. The cultivation of the seed culture in a disposable polymeric container was carried out in a liquid nutrient medium at a temperature of 26 to 28°C with continuous barbotage and mechanical agitation with a platform oscillation frequency of 80 to 90 per minute. For aeration, compressed air with a pressure of 0.3 to 0.4 kgf/cm2 was used. The volumetric flow rate of sterile air supplied for aeration ranged from 0.9 to 1.0 l/min.Results and discussion. The use of disposable polymeric containers made it possible to reduce the duration of the technological stage of obtaining a seed culture by 1.7 times and increase the yield of live microbes per unit volume of the nutrient medium by 2.8 times, as compared to the regulated production technology. Thus, the possibility and prospects of using disposable polymeric containers in the production technology of live plague vaccine at the stage of preparation of sowing cultures is evidenced.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"48 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Sequencing and Search for Determinants of Resistance to Benzalkonium Chloride in Burkholderia pseudomallei","authors":"D. N. Luchinin, D. Ustinov, I. Shpak, E. Molchanova","doi":"10.21055/0370-1069-2023-4-91-95","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-91-95","url":null,"abstract":"The aim of the study was to carry out whole-genome sequencing and comparative analysis of the original and benzalkonium chloride-resistant strains of Burkholderia pseudomallei.Materials and methods. We used the strain B. pseudomallei 134 and resistant to benzalkonium chloride B. pseudomallei 134K. Whole-genome sequencing was conducted on the MiSeq Reagent Kit v3 platform (600-cucle).Genome assembly for both strains was performed with the help of SPAdes v3.11.1. In order to compare genome sequences of the studied strains, Snippy v4.6.0 software was applied. MEGA X program was used to align the nucleotide and amino acid sequences.Results and discussion. The search and analysis of determinants responsible for the emergence of resistance to biocides in B. pseudomallei 134K have revealed two genes: the TetR transcriptional repressor gene and the AmrAB-OprA efflux pump gene. A single nucleotide polymorphism has been found in the TetR regulator, which led to the replacement of serine by proline in the mutant protein, and, as a result, a change in its secondary structure. It is believed that this mutation causes the loss of regulatory protein functionality, resulting in an increased expression of the efflux pump genes (AcrB/AcrD/AcrF) regulated by it. This follows by both, decrease in the level of sensitivity to benzalkonium chloride and the emergence of resistance to ceftazidime. In the AmrAB-OprA efflux pump gene, an insertion of 16 nucleotides has been detected at the position 544 of the amrA operon, which led to an increase in the length of the cistron, a shift in the reading frame, a change in the amino acid composition, and, as a result, a change in the secondary structure of the encoded protein. It is most likely that this mutation contributes to the loss of AmrAB-OprA operon function and the failure of normal outflow of xenobiotics from the cytoplasm of the microorganism. This assumption is evidenced by the loss of resistance to gentamicin in the mutant strain.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"24 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxotrophy of Yersinia pestis Strains of Antiqua Biovar and Its Genetic Basis","authors":"M. A. Makashova, L. M. Kukleva, N. Chervyakova, E. A. Naryshkina, A. V. Kovrizhnikov, G. A. Eroshenko, I. G. Shvidenko, V. V. Kutyrev","doi":"10.21055/0370-1069-2023-4-96-105","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-96-105","url":null,"abstract":"The aim of the work was to study the growth dependence on amino acids in Yersinia pestis strains of different phylogenetic branches of the antiqua biovar and to determine the genetic basis of auxotrophy of these strains.Materials and methods. We used 38 strains of Y. pestis, the main subspecies of the antiqua biovar, isolated in various foci of the world in the period of 1928–2020. The nutritional requirements of the strains were determined by incubation on Difco minimal agar with different sets of amino acids. Phylogenetic analysis of the strains was carried out using the Wombac 2.0 and SeaView 5.0.5 programs. Comparative analysis of the nucleotide sequences of the genes was performed using the BLAST algorithm and the Mega 7.0 program.Results and discussion. The phylogenetic appurtenance of the investigated Y. pestis strains of antiqua biovar to the phylogenetic branches 0.ANT3, 0.ANT5, 1.ANT, 2.ANT3, 3.ANT2, 4.ANT has been determined. It is established that all studied strains have a common dependence of growth on three amino acids – phenylalanine, threonine, and methionine. In the majority of the strains of antiqua biovar of all phylogenetic branches, growth is also dependent on the presence of cysteine in the medium, except for a part of the strains of the phylogenetic branch 4.ANT. In 14 genes of sulfur and cysteine metabolism, 19 mutations have been identified. Each phylogenetic group of the antique biovar has a distinct profile of mutations in genes involved in cysteine biosynthesis. The leucine requirement of the strains belonging to phylogenetic branch 0.ANT5 has been established, possibly caused by a frame shift in the leuA gene. Strains of the 1.ANT branch isolated in the Democratic Republic of the Congo demonstrate an additional proline-dependent growth. The defined nutritional requirements in the strains and the genetic causes of auxotrophy complement the phenotypic and genetic characteristics of the phylogenetic branches of the antiqua biovar and can be used as genetic markers for the differentiation of these strains.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Monitoring of Pandemic Influenza Virus Markers in the Russian Federation in 2021–2023","authors":"T. N. Ilyicheva, A. A. Moiseeva, K. Ivanova, M. S. Azaev, V. Marchenko","doi":"10.21055/0370-1069-2023-4-77-83","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-77-83","url":null,"abstract":"State Scientific Center of Virology and Biotechnology “Vector” has been monitoring highly pathogenic influenza since 2005.The aim of this work was to track the markers of highly pathogenic influenza in the blood sera of people who had a contact with infected and/or deceased birds, as well as of residents from regions where emergence of new variants of influenza A virus is most likely to occur.Materials and methods. Sera were studied using hemagglutination inhibition test (HI test). HI-positive sera were subjected to virus neutralization reaction.Results and discussion. In 2021, 2076 blood serum samples from 19 regions of Russia were collected. Only 7 samples demonstrated significant titers in HI test with A/H5N8 viruses. In 2022, 1620 blood serum samples from 23 regions were obtained; 25 of them were positive for influenza А/H5N8 and А/H5N1 viruses. In 2023 (January-August), 3335 serum samples from 31 regions of the Russian Federation were collected. 28 samples were positive for influenza А/H5N8 and А/H5N1 viruses. Furthermore, we monitored blood sera for low-pathogenic A/H9N2 virus. The number of positive samples in 2021 was lower than 1 % (13 out of 2076); in 2022, it reached 5 % (81 out of 1620); in 2023, the share was lower than 1 % (31 out of 3335). The data obtained suggest indirectly that currently there is no stable circulation of zoonotic influenza A/H5N8 and A/H5N1 viruses in Russia. Influenza viruses A/H9N2 have widely spread in many countries of the world and actively participate in evolution of highly pathogenic influenza A/H5Nx viruses. The Russian Federation demonstrates a gradual increase in the number of blood serum samples with antibodies to A/H9N2 virus.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"57 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern Features of Pathogenic Leptospira Isolation and Identification in Siberia and the Far East","authors":"N. V. Breneva, E. Y. Kiseleva, M. B. Sharakshanov, S. Borisov, S. E. Budaeva, S. Balakhonov","doi":"10.21055/0370-1069-2023-4-62-67","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-62-67","url":null,"abstract":"Recently, pathogenic Leptospira culture isolation is an extremely rare phenomenon in Russia.The aim of our work was to synthesize the lessons learned at the Irkutsk Anti-Plague Institute from Leptospira culture isolation and identification since 2011.Materials and methods. Material from eight individuals with suspected leptospirosis and from 942 small mammals (SM) was examined using PCR and microscopic agglutination test (MAT), from humans and 260 SM, applying bacteriological method. Bacteriological temperature test, MAT, PCR, MLST and MALDI-TOF mass spectrometry with the original Leptospira protein profiles base were used to identify cultures. Six complete genomes were generated at the Central Research Institute of Epidemiology of the Rospotrebnadzor.Results and discussion. Leptospira have not been isolated from humans against the background of taking antibiotics, despite the positive PCR and MAT results. Four cultures of Leptospira borgpetersenii of the Javanica serogroup and three L. kirschneri (Grippotyphosa) have been isolated from SM. The results of identification using MLST scheme No. 1 and MALDI-TOF MS are identical. MLST in silico has shown the uniformity of two Grippotyphosa serogroup strains from Primorie and Khabarovsk with a sequence-type (ST) profile 110:100:94. ST146 is determined in four Javanica serogroup strains according to scheme No. 1, and unique single nucleotide polymorphisms are detected according to schemes No. 2–3. Thus, in Siberia and the Far East, between 2012 and 2016, seven pathogenic Leptospira cultures were isolated from carriers in natural foci; carrier infection rate being12.0–48.9 %. Javanica serogroup strains differ in the MLST profile characteristics and adapt to nutrient media for a longer time than Grippotyphosa serogroup strains.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MLVA25 and CRISPR Genotypes of Yersinia pestis Strains from the Caspian Sandy Plague Focus","authors":"P. A. Goryunova, G. A. Eroshenko, A. N. Balykova, A. V. Kovrizhnikov, K. S. Shevchenko, L. M. Kukleva, E. A. Naryshkina, N. Chervyakova, V. V. Kutyrev","doi":"10.21055/0370-1069-2023-4-68-76","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-68-76","url":null,"abstract":"The aim of the work was to study the genetic diversity and spatial-temporal structure of Yersinia pestis in the Caspian sandy plague focus using MLVA25 and CRISPR typing methods.Materials and methods. 98 Y. pestis strains isolated in the territory of the Caspian sandy plague focus in 1925–2015 were used in the study. Whole genome sequencing was performed on Ion GeneStudio S5 System (ThermoFisher Scientific) and MGI (DNBSEQ-G50RS) platforms. Fragment sequencing was conducted using “ABI PRISM 3500XL”. The search for VNTR and CRISPR loci with subsequent alignment of nucleotide sequences was carried out in the MEGA X program. The obtained sequences were entered into the created database in the Bionumerics v7.6 (Applied Maths) software. The phylogenetic tree was constructed using the UPGMA method.Results and discussion. According to the results of MLVA25 and CRISPR analysis, 98 Y. pestis strains are divided into 60 genotypes (CS1 – CS60). Variability by 23 VNTR loci has been discovered. 7 new CRISPR spacers are described: 5 in YPa and 2 in YPb (size 31–33 bp, GC composition 34–58 %). The described spacers are designated as a108, a109, a110, a111, a112, b53, b54. The interrelation of changes in the copy number of VNTR loci depending on the place and time of isolation of strains has been revealed. The data obtained can be used to carry out molecular-genetic certification of the territory of the Caspian sandy plague focus and to study the routes and patterns of evolution and spatiotemporal circulation of Y. pestis populations in the Caspian plague foci.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Resistance Patterns in Shiga Toxin-Producing Escherichia coli (STEC), Salmonella, Shigella and Staphylococcus aureus Isolated at Two Communal Kitchens Located in Hanoi City, Vietnam","authors":"Thi Van Anh Le, Thi Loan Ta, Thu Minh Dinh, Thi Thuong Vu, Thi Ha Giang Pham, Thi Thanh Nga Bui, Viet Hung Pham, Ngoc Tan Nguyen, Phi Long Trieu, Thi Van Anh Le, Dang Hieu Hoang","doi":"10.21055/0370-1069-2023-4-84-90","DOIUrl":"https://doi.org/10.21055/0370-1069-2023-4-84-90","url":null,"abstract":"The aim of our study was to investigate the antibiotic resistance profiles of Shiga toxin-producing Escherichia coli (STEC), Salmonella spp., Shigella spp., and Staphylococcus aureus strains.Materials and methods. 660 samples were collected at two communal kitchens in Hanoi, Vietnam between 2021 and 2022. They included foodstuffs, environmental (food processing tools) and biological ones (swabs from the hands of personnel). The VITEK® 2 Compact system in combination with DNA sequencing was used to identify bacterial species. The antibiotic susceptibility test (AST) was performed according to Kirby-Bauer Disk Diffusion Susceptibility protocol following Clinical & Laboratory Standards Institute (CLSI) method (M100-Ed32).Results and discussion. In total, 53 pathogenic bacterial strains have been detected, including 11 STEC, 24 Salmonella enterica, 9 Shigella sonnei, Shigella flexneri, and 8 S. aureus. AST of STEC has showed the highest resistance rates to tetracycline and chloramphenicol (90.9 %); trimethoprim+sulfamethoxazole (81.8 %); ampicillin, gentamycin and piperacillin (63.6 %). The STEC isolates were susceptible to carbapenem group. Among the Salmonella strains, 50 % demonstrated resistance to ampicillin, followed by tetracycline and piperacillin (45.8 %). Additionally, 25 % were resistant to ticarcillin+clavulanic acid, 20.8 % – to trimethoprim+sulfamethoxazole, and 16.7 % – to chloramphenicol. All Salmonella strains exhibited susceptibility to gentamicin, cefoxitin, imipenem, meropenem, and ceftazidime. AST of Shigella strains revealed the highest resistance rate for tetracycline (30 %), followed by cefazolin and ceftazidime (20 %). However, all Shigella strains were susceptible to cefoxitin, carbapenem groups, and chloramphenicol. Among the S. aureus strains, 50 % exhibited resistance to erythromycin, azithromycin, clindamycin, penicillin, telithromycin, and gentamicin, followed by ciprofloxacin, moxifloxacin, levofloxacin, and chloramphenicol (25 %). All S. aureus strains were still susceptible to trimethoprim+sulfamethoxazole, daptomycin, linezolid, doxycycline, minocycline, and vancomycin. Our findings reflect the current situation on antibiotic resistance among pathogenic bacteria strains circulating at the study sites during food processing. They are an evidence of potential risk of food poisoning. There is a need to undertake the proper containment measures on the part of authorities or policy makers.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"63 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}