The Natural Products Journal最新文献

{"title":"Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats","authors":"Zurain Radjeni, Mardiana Abdul Aziz, Norasikin Ab Azis, Mohd Saleh Ahmad Kamal, Harbindar Jeet Singh","doi":"10.2174/0122103155281988231211105638","DOIUrl":"https://doi.org/10.2174/0122103155281988231211105638","url":null,"abstract":"\u0000\u0000Laboratory evidence pertaining to the antihypertensive activity of a number\u0000of Ficus deltoidea (FD) varieties remains undetermined, although extracts of some of these varieties\u0000have been shown to have angiotensin-converting enzyme (ACE) inhibitory activity in vitro.\u0000\u0000\u0000\u0000This study, therefore, evaluated the effect of a standardized aqueous-ethanolic extract of\u0000leaves of Ficus deltoidea var. trengganuensis (FDT) on blood pressure in spontaneously hypertensive\u0000rats (SHR).\u0000\u0000\u0000\u0000SHR were given either to 800, 1000 or 1200 mg kg-1 body weight of standardized aqueous-\u0000ethanolic extract of FDT or 10 mg kg-1 body weight of losartan or 0.5 ml of distilled water daily\u0000for four weeks.\u0000\u0000\u0000\u0000SBP decreased significantly in FDT- and losartan-treated rats (p < 0.05). Components of\u0000RAAS and other serum and urinary parameters were not different between the groups except for\u0000Endothelin-1, which was significantly lower than that in the controls. Urinary calcium excretion was\u0000significantly higher in FDT-treated rats (p < 0.05).\u0000\u0000\u0000\u0000It seems that daily oral administration of FDT significantly lowers blood pressure in\u0000SHR through mechanisms that do not involve RAAS but may involve the endothelium or other yetto-\u0000be-determined mechanisms.\u0000","PeriodicalId":515404,"journal":{"name":"The Natural Products Journal","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139628520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Studies of Alstonia scholaris, Chemical Transformation and\u0000Biological Evaluation against a Breast Cancer Cell Line","authors":"Salome Ghansenyuy, Eyong Kenneth Oben, P. Yemback, Nziko Vincent de Paul Nzuwah, Ali Muhammad Shaiq., Folefoc Gabriel Ngosong., Davis Samantha, Tobin Jenna, Haleigh Parker, Taube Joseph","doi":"10.2174/0122103155260546231113052349","DOIUrl":"https://doi.org/10.2174/0122103155260546231113052349","url":null,"abstract":"\u0000\u0000Some Alstonia species are used in traditional medicine to treat diseases such\u0000as cancer, dysentery, diarrhea, jaundice, malaria, gastrointestinal troubles, and snake-bites.\u0000\u0000\u0000\u0000In this study, we aim to evaluate the ethanol leaf extract of Alstonia scholaris for anticancer\u0000constituents and structural modification to introduce a privilege medicinal α,β-unsaturated\u0000scaffold.\u0000\u0000\u0000\u0000The relative viability of the MDA-MB-231 breast cancer cell line exposed to isolated\u0000compounds at different concentrations was assayed. Chemical analysis was carried out by high resolution\u0000mass spectrometry and one and two-dimensional NMR techniques.\u0000\u0000\u0000\u0000Structures of purified compounds were determined as betulin 1, α-amyrin acetate 2, mixture of\u0000β-sitosterol 3 and stigmasterol 4, tetratriacontyl-trans-p-coumarate 5, ursolic acid 6, β-sitosterol glucoside\u00007, picralstonine 8 and scholaricine 9. To introduce privilege medicinal scaffold, compounds 1 and 2\u0000under SeO2 oxidation condition afford new acrylaldehye derivatines. Compound 1 afforded Betulin\u0000acrylaldehyde 10 while compound 2 afforded lupeolacetate acryl aldehyde 11 in an intriguing mechanism\u0000with the conversion of ursane to lupane scafford. Compound 11 equally showed interesting activity\u0000against MDA MB 231 breast cancer cell line with an IC50 of 4.63 ± 0.09 μg/ml.\u0000\u0000\u0000\u0000From these findings, the medicinal α,β-unsaturated scaffold could have pharmacological\u0000effects in treating MDA-MB-231 breast cancer.\u0000","PeriodicalId":515404,"journal":{"name":"The Natural Products Journal","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity and Antitumor Potential of Hedyotis chrysotricha Extract in Human Colorectal Cancer and Breast Cancer Cell Lines","authors":"Jing Zhang, Shannuo Li, Qianqian Lv, Jiahui Lin, Ying Xiao, Jiajie Liang, Tianwan Zhang, Lili Zeng, Peiting Guo, Hong Ji","doi":"10.2174/0122103155275580231124052950","DOIUrl":"https://doi.org/10.2174/0122103155275580231124052950","url":null,"abstract":"\u0000\u0000Cancer is a serious disease severely endangering human health and life in\u0000the world in the world, and conventional anticancer drugs have frequently suffered from severe side\u0000effects and resistance. Therefore, developing novel and effective therapeutic strategies is urgently\u0000needed. As a Chinese herbal medicine with important medical values, Hedyotis chrysotricha has\u0000been suggested to be useful for the treatment of many diseases, especially cancer.\u0000\u0000\u0000\u0000Cancer is one of the leading causes of death in the world, and conventional anticancer drugs have frequently suffered from severe side effects and resistance. Therefore, developing novel and effective therapeutic strategies is urgently needed. As a Chinese herbal medicine with important medical values, Hedyotis chrysotricha was suggested to be useful for the treatment of many diseases, especially cancer.\u0000\u0000\u0000\u0000Our aim was to investigate the antioxidant and antitumor activities of the phytochemical\u0000extracts of H. chrysotricha.\u0000\u0000\u0000\u0000The purpose of the present study was to investigate the antioxidant activity and antitumor properties of the phytochemical extracts of Hedyotis chrysotricha.\u0000\u0000\u0000\u00002,2-diphenyl-1-picrylhydrazyl free radical scavenging assay was applied to determine\u0000antioxidant property. 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay, wound\u0000healing assay, transwell matrix assay, flow cytometry and cell morphology were established to observe\u0000anti-proliferative, anti-migration, anti-invasion and apoptosis induction effects. The intracellular\u0000reactive oxygen species generation was detected by reactive oxygen species detection assay.\u0000\u0000\u0000\u0000In this study, 2,2 diphenyl 1 picrylhydrazyl (DPPH) free radical scavenging assay was used to determine antioxidant activity. MTT, wound healing assay, transwell matrix assay, flow cytometry and cell morphology were established to observe anti-proliferative, anti-migration, anti-invasion and apoptosis induction effects. The intracellular reactive oxygen species (ROS) generation was measured using ROS detection assay.\u0000\u0000\u0000\u0000The ethyl acetate fraction showed promising antioxidant activity with IC50 of 98.67±0.49\u0000μg/ml, and petroleum ether fraction exhibited the most potent antiproliferative effect on various human\u0000cancer cell lines, especially MDA-MB-231 (IC50 = 8.90±1.23 μg/ml) and HCT-116 (IC50 =\u00009.69±3.69 μg/ml) cancer cells. Further investigation revealed that the petroleum ether fraction suppressed\u0000the proliferation, migration and invasion of the cancer cells significantly. Additionally, it\u0000promoted cell apoptosis by increasing reactive oxygen species levels.\u0000\u0000\u0000\u0000It was demonstrated that the ethyl acetate fraction had promising antioxidant activity with IC50 of 98.67±0.49 μg/ml, whereas petroleum ether fraction exhibited the most potent anti-proliferative activity against various human cancer cell lines, especially MDA-MB-231 (IC50 = 8.90±1.23 μg/ml) and HCT-116 (IC50 = 9.69±3.69 μg/ml) cancer cells. Further investigation revealed that the petroleu","PeriodicalId":515404,"journal":{"name":"The Natural Products Journal","volume":"32 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Secondary Metabolites Produced by the Hadal Actinomycete<i>Streptomyces</i> sp. SY1414 Isolated from the Mariana Trench-derived Sediment","authors":"Di Zhang, Yao Feng, Huifang Li, Zhizhen Zhang","doi":"10.2174/0122103155281050231123050658","DOIUrl":"https://doi.org/10.2174/0122103155281050231123050658","url":null,"abstract":"Background:: Accumulated investigations have demonstrated that the Mariana Trench is enriched in microorganisms. However, the diversity of structures and bioactivities of the secondary metabolites produced by the Mariana Trench-associated microorganisms is poorly known, which needs to be intensively investigated. Objective:: This study aimed to investigate the bioactive secondary metabolites produced by a Mariana Trench-derived actinomycete Streptomyces sp. SY1414 was cultured in BY medium, which was chosen from four different media based on the diversity of secondary metabolites. Methods:: A combination of different column chromatographs and HPLC was applied for the separation and purification of the secondary metabolites. The structures of the isolated compounds were determined mainly based on their NMR data, optical rotation values, and the comparison with the reference data, and the Sulforhodamine B (SRB) method was used to evaluate the anti-glioma activity of isolated compounds. Results:: Four different types of compounds were isolated from the large culture of strain Streptomyces sp. SY1414 in BY medium, including a benzoquinoline alkaloid, actinophenanthroline C (1), a benzamide, (2E,4E)-5-(3-hydroxyphenyl)-penta-2,4-dienamide (2), a cyclopeptide, valinomycin (3), and four macrolides of bafilomycin D (4), bafilomycin A2 (5), bafilomycin W (6), and C(19), C(21)- O-methyl-bafilomycin A1 (7). Actinophenanthroline C (1), bafilomycin D (4), bafilomycin A2 (5), and bafilomycin W (6) displayed significant anti-glioma activities with IC50 values ranging from 1.62 to 8.20 μM for U87MG cells and 2.45 to 3.89 μM for U251. The anti-gliomas of actinophenanthroline C (1) was reported for the first time. Conclusion:: The hadal actinomycete Streptomyces sp. SY1414 in BY medium produced four different types of secondary metabolites with significant anti-glioma activity, which enriched the diversity of structures and bioactivities of the Mariana Trench-associated natural products.","PeriodicalId":515404,"journal":{"name":"The Natural Products Journal","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Composition and Antioxidant Activity of Thymus fontanesii Essential Oil from Algeria","authors":"Sid Ali Lamia, B. Moussa, Fauconnier Marie-Laure, Lognay Georges","doi":"10.2174/2210315508666180427162542","DOIUrl":"https://doi.org/10.2174/2210315508666180427162542","url":null,"abstract":"\u0000\u0000Thymus fontanesii is one of the important Algerian plants, used traditionally\u0000to treat the cough and cold. In addition, it may help to protect the people against lipid peroxidation\u0000and oxidative stress and can be used as an antioxidant agent for the preservation of processed\u0000food.\u0000\u0000\u0000\u0000The aim of this study was to determine the chemical composition of Algerian Thymus\u0000fontanesii essential oil and to test its antioxidant activity.\u0000\u0000\u0000\u0000The oil was extracted by electromagnetic induction (EMI) heating assisted extraction and\u0000by hydrodistillation, and was analysed by Gas Chromatography with Flame Ionization Detector\u0000(GC/FID) and Gas Chromatography/Mass Spectrometry (GC/MS). The antioxidant activity was\u0000evaluated by three assays mainly: DPPH assay, reducing power and β-carotene/linoleic acid.\u0000\u0000\u0000\u0000The yield of the essential oil was varied from 2.1 ± 0.3 to 3.1 ± 0.1% (w/w), and from 1.8 ±\u00000.01 to 2.6 ± 0.02% (w/w), for the electromagnetic induction heating assisted extraction and hydrodistillation,\u0000respectively. Twenty seven components were identified representing 95.6 - 99.9% of the\u0000oil. Carvacrol (54.7 ± 1.2 - 63.9 ± 1.9%) was the major compound followed by p-cymene (9.2 ± 1.2\u0000- 17.5 ± 1.2%) and γ-terpinene (8.8 ± 0.9 - 14.9 ± 0.8%). The Thymus fontanesii essential oil was\u0000found as a significant antioxidant with IC50 values ranging from 57.3 ± 1.4 to 236.7 ± 1.4 μg/mL,\u0000which were higher than that of butylated hydroxyl toluene (BHT) choosing as reference (9.1 ± 1.2 to\u000067.8 ± 0.1 μg/mL).\u0000\u0000\u0000\u0000The obtained results encourage the use of Thymus species with bioactive compounds\u0000for further food applications.\u0000","PeriodicalId":515404,"journal":{"name":"The Natural Products Journal","volume":" 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141219992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}