Horticulture Journal最新文献_第3页

Factors Explaining Variations in Soluble Solids Content of Apples during Ripening and Storage 解释苹果在成熟和贮藏过程中可溶性固形物含量变化的因素

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-03-16 DOI: 10.2503/hortj.qh-105

Hiroshi Iwanami, Yuki Moriya-Tanaka, Toshio Hanada, Takashi Baba, Daisuke Sakamoto

{"title":"Factors Explaining Variations in Soluble Solids Content of Apples during Ripening and Storage","authors":"Hiroshi Iwanami, Yuki Moriya-Tanaka, Toshio Hanada, Takashi Baba, Daisuke Sakamoto","doi":"10.2503/hortj.qh-105","DOIUrl":"https://doi.org/10.2503/hortj.qh-105","url":null,"abstract":"Sweetness is one of the most important drivers of consumer preference in apples (Malus × domestica Borkh.). The increase in sugar during the ripening period of fruit is mainly brought about by the hydrolysis of starch accumulated before the ripening period. However, sugars are also continuously translocated into the fruit during the ripening period, as seen in watercored fruits. The objective of this study was to estimate the contribution of translocated sugars that accumulated in the apoplast to the increase in soluble solids content (SSC) of fruit during ripening. The amount of apoplastic solution (AS) tended to be high in the fruit of trees on vigorous rootstocks, such as ‘JM2’ and ‘Marubakaido’. On the other hand, fruit with more AS had lower SSC. Therefore, although AS increased during ripening, the contribution of AS to the increase in SSC was small. After fruit matured and during storage, dehydration increased the SSC of the fruit. On the other hand, the SSC decreased simultaneously due to a reduction in organic acids and any soluble solids, which was expressed as a decrease in titratable acidity (TA). Under standard refrigerated conditions, the increase in SSC due to dehydration and the decrease in SSC due to respiration were likely to be quantitatively comparable. The contribution of translocated sugars to the increase in SSC during ripening was small, suggesting that managing trees and fruit to increase starch accumulation before fruit ripening is crucial for the production of fruits with high sugar content.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Floral Scent Components of Roses Exhibiting an Unusual Odor: Identification of Stink Bug Pheromone Compounds in Roses 分析具有异常气味的玫瑰的花香成分：玫瑰中臭虫信息素化合物的鉴定

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-03-16 DOI: 10.2503/hortj.qh-130

Naomi Oyama-Okubo, Yuki Mikanagi

{"title":"Analysis of Floral Scent Components of Roses Exhibiting an Unusual Odor: Identification of Stink Bug Pheromone Compounds in Roses","authors":"Naomi Oyama-Okubo, Yuki Mikanagi","doi":"10.2503/hortj.qh-130","DOIUrl":"https://doi.org/10.2503/hortj.qh-130","url":null,"abstract":"Flowers of the early Polyantha roses, ‘Pâquerette’, ‘Mignonette’ and ‘Anne-Marie de Montravel’, have an unusual odor not found in other roses. Their ancestor is Rosa multiflora, a wild species native to Japan. In particular, ‘Anne-Marie de Montravel’ has a citrus scent with an old wooden closet-like odor. Gas chromatography (GC)-mass spectrometry analysis of the scent components of these roses revealed the presence of several terpenoids, benzenoids, and hydrocarbons, as well as the fatty acid derivatives methyl (E, Z)-2,4-decadienoate (MDD) and methyl (E, E, Z)-2,4,6-decatrienoate (EEZ-MDT). GC-olfactometry analysis of scent components of ‘Anne-Marie de Montravel’, identified EEZ-MDT and its isomers as the source of the unpleasant odor. MDD and EEZ-MDT are known as stink bug pheromones, but they were first detected in the scent of plants. We also analyzed the floral scent of several roses related to R. multiflora, namely: two plants of R. multiflora, two Hybrid Multiflora roses (‘Crimson Rambler’ and ‘Rose-Marie Viaud’), eight Polyantha roses (‘Cécile Brunner’, ‘Clothilde Soupert’, ‘Gloire des Polyantha’, ‘Madame Norbert Levavasseur’, ‘Marie Daly’, ‘Marie Pavié’, R. multiflora ‘Nana’, ‘Yvan Misson’), and one Miniature rose (‘Gourmet Popcorn’). EEZ-MDT and/or its isomers were detected not only in ‘Marie Daly’, ‘Marie Pavié’, R. multiflora ‘Nana’, and ‘Gourmet Popcorn’, which had an old wooden closet-like odor, but also in R. multiflora, ‘Rose-Marie Viaud’ and ‘Yvan Misson’, which do not exhibit that unpleasant odor. To produce the unusual odor in the rose fragrance, the EEZ-MDT and its isomers content must be above a certain percentage of the scent components.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140156452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a Gene Conferring Moderate Susceptibility to Alternaria Blotch (Alternaria alternata apple pathotype) in ‘Golden Delicious’ Apples (Malus × domestica Borkh.) 鉴定 "金美味 "苹果（Malus × domestica Borkh.）中对交替孢属斑点病（交替孢属苹果病原型）具有中度易感性的基因

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-03-12 DOI: 10.2503/hortj.qh-134

Shigeki Moriya, Kazuyuki Abe, Kazuma Okada, Taku Shimizu, Miyuki Kunihisa, Ayato Hori, Yutaka Sawamura

{"title":"Identification of a Gene Conferring Moderate Susceptibility to Alternaria Blotch (Alternaria alternata apple pathotype) in ‘Golden Delicious’ Apples (Malus × domestica Borkh.)","authors":"Shigeki Moriya, Kazuyuki Abe, Kazuma Okada, Taku Shimizu, Miyuki Kunihisa, Ayato Hori, Yutaka Sawamura","doi":"10.2503/hortj.qh-134","DOIUrl":"https://doi.org/10.2503/hortj.qh-134","url":null,"abstract":"Alternaria blotch, a major apple (Malus × domestica Borkh.) fungal disease in Japan, is caused by the Alternaria alternata apple pathotype that produces a host-selective toxin called AM-toxin. Although control of Alternaria blotch currently relies on spring-to-summer fungicide use, there is a growing need for sustainable agriculture practices that reduce chemical inputs in orchards. Therefore, breeding cultivars for resistance to Alternaria blotch is of particular interest. Given that ‘Golden Delicious’ (GD) and several of its offspring cultivars are moderately susceptible to the disease, a genetic analysis of their susceptibility was performed. Quantitative trait locus (QTL) analysis of the ‘Fuji’ × ‘GD’ population identified a single QTL on chromosome 11 in ‘GD’, which explained 48.7% of the phenotypic variation. This QTL was located in the same region as the previously identified susceptibility gene Alt derived from ‘Starking Delicious’. Therefore, we named it QTL Alt2, a putative allele of Alt (later renamed Alt1). Interaction analysis revealed that Alt1 was dominant over Alt2. A DNA marker set that simultaneously detects Alt1 and Alt2 was developed for breeding use. This marker shed light on the inheritance of the Alt locus in modern Japanese cultivars and selections. Moreover, Alt2 was less common than Alt1 in heirloom cultivars. These findings offer new insights into apple breeding for Alternaria blotch resistance and the interaction mechanism between apple and host-selective toxin-producing A. alternata.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140107085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lisianthus Flowers Emitted Volatile Components Including Iridoids and Actinidine Which Attract Cats 桔梗花释放出的挥发性成分，包括吸引猫的类铱和放线菌素

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-03-12 DOI: 10.2503/hortj.qh-112

Naomi Oyama-Okubo, Naoko Fukuta

{"title":"Lisianthus Flowers Emitted Volatile Components Including Iridoids and Actinidine Which Attract Cats","authors":"Naomi Oyama-Okubo, Naoko Fukuta","doi":"10.2503/hortj.qh-112","DOIUrl":"https://doi.org/10.2503/hortj.qh-112","url":null,"abstract":"Lisianthus (Eustoma grandiflorum [Raf.] Shinners) is one of the world’s major cut flowers, characterized by its wide variety of flower colors, flower shapes, long stem, and long vase life. Lisianthus is said to be scentless, but there are cultivars that have a weak or faint scent. Cats exhibit a characteristic response to lisianthus flowers similar to their response to Actinidia polygama leaves, which have a very weak scent for humans. These observations suggested that the scent of lisianthus flowers may have a component that attracts cats. The volatile components of Eustoma ‘New Lination White’ flowers, which has a weakly sweet scent, and 12 lisianthus cultivars, which have a very faint scent, were analyzed. Thirty-six kinds of volatile components were detected in the flowers of ‘New Lination White’, including four iridoid compounds (nepetalactone, isodihydronepetalactone, iridomyrmecin, and isoiridomyrmecin) and actinidine, which have been recognized as attracting cats. The major volatile components are sesquiterpenes, and phenypropanoids such as eugenol were identified as components with a sweet scent. Iridoid compounds and actinidine were detected only in flowers, but not in leaves or stems. In addition, iridoid compounds were detected in all 12 cultivars analyzed. Lisianthus flowers were thought to be scentless but we identified many volatile components, including iridoid compounds and actinidine, that attracts cats. This research is the first report on the scent of lisianthus flowers.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140107082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Gibberellin and an Ethylene Inhibitor on Twining of Vine Cuttings in Japanese Morning Glory (Ipomoea nil (L.) Roth) 赤霉素和乙烯抑制剂对日本牵牛花（Ipomoea nil (L.) Roth）扦插藤条缠绕的影响

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-02-07 DOI: 10.2503/hortj.qh-108

Tomoe Yofune, Nanami Matsumoto, Miyuki Funamoto, Tsuyoshi Kaneta

{"title":"The Effect of Gibberellin and an Ethylene Inhibitor on Twining of Vine Cuttings in Japanese Morning Glory (Ipomoea nil (L.) Roth)","authors":"Tomoe Yofune, Nanami Matsumoto, Miyuki Funamoto, Tsuyoshi Kaneta","doi":"10.2503/hortj.qh-108","DOIUrl":"https://doi.org/10.2503/hortj.qh-108","url":null,"abstract":"“Twining” along other objects is an important morphogenetic survival response of vine plants. The twining response, induced by the stimulus of touching a supporting object, is a form of “thigmomorphogenesis”. Ethylene is thought to play an important role in thigmomorphogenesis in higher plants, so it is likely to be involved in vine twining. However, the relationship between ethylene and vine twining is not well understood. We used vine cuttings excised from morning glory (Ipomoea nil (L.) Roth ‘Violet’) plants in order to investigate the effect of an ethylene inhibitor on elongation and twining. The vine cuttings required gibberellin for elongation and twining. In the vine cuttings with elongation and twining induced by gibberellin treatment, treatment with 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, significantly decreased the angle of rotation and the spiral pitch of the twining. These results suggest that ethylene may be involved in either sensing a pole via touch and the morphological changes during vine twining or both. We also selected one of the genes for 1-aminocyclopropane-1-carboxylic acid synthase (ACS), which is a key enzyme in the ethylene biosynthetic pathway, with higher expression in tissues on the side touching the pole in twining vines as one of the candidate genes thought to be involved in vine twining in morning glory.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Malformed Incurved Flowers in the Cut Rose Cultivar ‘Yves Piaget’ by Methyl Jasmonate Spray Treatment of Flower Buds before Harvest 收获前对花蕾进行茉莉酸甲酯喷雾处理对切花玫瑰品种 "伊夫-伯爵 "畸形内弯花的抑制作用

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-02-07 DOI: 10.2503/hortj.qh-119

Rei Kaneeda, Yuri Kanno, Mitsunori Seo, Keith Hardie, Takashi Handa

{"title":"Inhibition of Malformed Incurved Flowers in the Cut Rose Cultivar ‘Yves Piaget’ by Methyl Jasmonate Spray Treatment of Flower Buds before Harvest","authors":"Rei Kaneeda, Yuri Kanno, Mitsunori Seo, Keith Hardie, Takashi Handa","doi":"10.2503/hortj.qh-119","DOIUrl":"https://doi.org/10.2503/hortj.qh-119","url":null,"abstract":"In the most popular fragrant rose cultivar in Japan, ‘Yves Piaget’, the petal edges are frequently malformed, curving toward the adaxial side. These malformed petals prevent normal flowering and weaken the flower fragrance, which significantly decreases the quality of this cultivar and increases financial losses of cut flowers. We refer to such malformed flowers as ‘incurved flowers’. It has been reported that jasmonic acid (JA) affects petal growth. Therefore, we attempted to control the number of incurved flowers by applying exogenous JA, methyl jasmonate (MeJA), during flower development before harvest. Two types of spray treatment were applied to the flower buds before flower opening; (1) 100 μM MeJA or (2) deionized water as a control. The 100 μM MeJA spray treatment before harvest reduced the incurved flower rate, with fewer incurved petals, and resulted in a significantly larger maximum flower diameter and longer stamen length. In addition, the 100 μM MeJA spray treatment before harvest tended to increase the number of days from the commercial harvest stage to full bloom and also significantly increased the maximum flower diameter of fully-bloomed flowers. We also analyzed the endogenous phytohormone content in the petals of normal and incurved flowers at each flower developmental stage. The results showed that at the beginning of the flower opening stage the petals of incurved flowers had higher indoleacetic acid (IAA) content and lower JA/jasmonoyl isoleucine (JA-Ile) content than those of normal flowers. In particular, the JA and JA-Ile contents in incurved petals were approximately one quarter of those in normal flowers. These results suggest that IAA, JA, and JA-Ile may be involved in the development of incurved flowers.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Vase Life of Cut Gerbera Flowers is Extended by Combined Treatment with Gibberellin A3 and Calcium Chloride 赤霉素 A3 和氯化钙联合处理可延长非洲菊切花的花瓶寿命

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-01-31 DOI: 10.2503/hortj.qh-109

Makoto Tonooka, Akari Iriya, Kazuo Ichimura

{"title":"The Vase Life of Cut Gerbera Flowers is Extended by Combined Treatment with Gibberellin A3 and Calcium Chloride","authors":"Makoto Tonooka, Akari Iriya, Kazuo Ichimura","doi":"10.2503/hortj.qh-109","DOIUrl":"https://doi.org/10.2503/hortj.qh-109","url":null,"abstract":"Treatment with calcium chloride (CaCl2) is known to suppress the occurrence of flower stem bending and extend the vase life of cut gerbera. To clarify whether vase life extension by CaCl2 is improved by combined treatment with gibberellin A3 (GA3), the effect of treatment with 50 mg·L−1 GA3, 5 g·L−1 CaCl2 or in combination on the vase life of the cut gerbera ʻMinouʼ was investigated. To inhibit bacterial proliferation, which is known to shorten vase life, an isothiazoline antimicrobial compound was included in the vase solution. Treatment with GA3 alone delayed the opening of tubular florets and increased the area of unopen florets, but stem elongation which led to stem bending shortened vase life. Treatment with GA3 in combination with CaCl2 suppressed the occurrence of stem bending. Combined treatment with GA3 and CaCl2 extended the vase life of cut gerbera more than treatment with CaCl2 alone. To clarify whether GA3 delays petal senescence, the effect of GA3 at 10 and 50 mg·L−1 on petal senescence was investigated using shortened stems. GA3 at both concentrations significantly delayed petal senescence. Combined treatment with GA3 and CaCl2 also significantly extended the vase life of the cut gerbera ʻKimseyʼ and ʻSandyʼ. It was concluded that combined treatment with GA3 and CaCl2 is a suitable treatment for extending the vase life of cut gerbera.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139584984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthocyanins and Flavonols from the Flowers of Ranunculus Cultivars (Ranunculaceae) and Their Color Expression 毛茛科植物毛茛花的花青素和黄酮醇及其色彩表现

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-01-23 DOI: 10.2503/hortj.qh-098

Qi Qin, Fumi Tatsuzawa, Takahisa Nakane, Takashi Kaidzuka, Tsukasa Iwashina, Takayuki Mizuno

{"title":"Anthocyanins and Flavonols from the Flowers of Ranunculus Cultivars (Ranunculaceae) and Their Color Expression","authors":"Qi Qin, Fumi Tatsuzawa, Takahisa Nakane, Takashi Kaidzuka, Tsukasa Iwashina, Takayuki Mizuno","doi":"10.2503/hortj.qh-098","DOIUrl":"https://doi.org/10.2503/hortj.qh-098","url":null,"abstract":"Isolation, purification and identification of anthocyanins and flavonols were carried out on flowers of Ranunculus cultivars. Three anthocyanins and 11 flavonols were characterized by chemical and spectroscopic techniques. The anthocyanins were identified as cyanidin 3-O-sambubioside, cyanidin 3-O-(6''-malonylsambubioside) and delphinidin 3-O-(6''-malonylsambubioside). The flavonols were identified as 3-O-glucosides and 3-O-sophorosides of kaempferol and quercetin, and their acylated compounds with malonic acid. Flower colors were divided into six groups, Red-Purple, White, Yellow-Orange, Orange, Red, and Violet groups using the Royal Horticultural Society Colour Chart. The absorption maxima of buffer solutions containing anthocyanins and flavonols isolated in this study were measured to understand the effect of intermolecular copigmentation between these compounds on flower color. The results showed that by addition of 3-O-(6''-malonylglucoside) of kaempferol or quercetin, the absorption maximum of cyanidin 3-O-(6''-malonylsambubioside) or delphinidin 3-O-(6''-malonylsambubioside) shifted bathochromically and exhibited a closer absorption maximum to fresh flower petals than anthocyanin alone. This indicates that the intermolecular copigment effect between anthocyanins and flavonols is responsible for the flower color expression in Ranunculus cultivars.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139557705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An 18.5-kbp Deletion in the Genomic Region, Including the Pun1, is Responsible for Non-pungency Traits in the Japanese Sweet Pepper  ‘Sampo Oamanaga’ (Capsicum annuum) 日本甜椒 "Sampo Oamanaga"（Capsicum annuum）中包括 Pun1 在内的基因组中 18.5-kbp 的缺失是导致其无辛辣性状的原因

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2024-01-16 DOI: 10.2503/hortj.qh-100

Moe Yamaguchi, Kondo Fumiya, Kazuhiro Nemoto, Kenichi Matsushima

{"title":"An 18.5-kbp Deletion in the Genomic Region, Including the Pun1, is Responsible for Non-pungency Traits in the Japanese Sweet Pepper \u2028‘Sampo Oamanaga’ (Capsicum annuum)","authors":"Moe Yamaguchi, Kondo Fumiya, Kazuhiro Nemoto, Kenichi Matsushima","doi":"10.2503/hortj.qh-100","DOIUrl":"https://doi.org/10.2503/hortj.qh-100","url":null,"abstract":"Capsaicinoids are compounds that generate the characteristic pungent taste of chili peppers, the presence or absence of which determines the utilization of the chili peppers as spices or vegetables. Loss of pungency is a qualitative trait resulting from dysfunction in any of four capsaicinoid biosynthesis genes (Pun1, pAMT, CaKR1, and CaMYB31). However, the lack of pungency in sweet peppers cannot be explained by known mutation alleles in these four genes. Herein, we report a novel dysfunctional allele of Pun1, which encodes acyltransferase 3 (capsaicin synthase), in a Japanese sweet pepper, ‘Sampo Oamanaga’. Firstly, PCR genotyping of ‘Sampo Oamanaga’ Pun1 showed that it was not a known mutant allele. We also performed whole-genome resequencing and found a large genomic deletion around the ‘Sampo Oamanaga’ Pun1 (XM_016704778.1). We then examined the precise size and breakpoint of the Pun1-deletion region via de novo assembly and Sanger sequencing analysis. We found an 18.5-kbp deletion, including the Pun1, on chromosome 2, and we designated this novel allele pun15. The genotypic effects of pun15 were investigated using F2 progeny derived from a ‘Sampo Oamanaga’ (pun15/pun15) × pungent cultivar ‘Takanotsume’ (Pun1/Pun1) cross. Only pun15-homozygous F2 plants showed non-pungency; co-segregation between Pun1 genotypes and pungency traits was observed. These results demonstrated that the deficiency of pungency in ‘Sampo Oamanaga’ is associated with the pun15 allele. The present study is the first to discover a large genomic deletion, including a gene among dysfunctional pun1 alleles, and provides new insights into the regulation mechanism of pungency in chili peppers.\u0000","PeriodicalId":51317,"journal":{"name":"Horticulture Journal","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139482789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthocyanin Composition of the Purple Sweet Potato Cultivar ‘Churakanasa’ 紫甘薯栽培品种 "Churakanasa "的花青素成分

IF 1.2 4区农林科学

Horticulture Journal Pub Date : 2023-12-28 DOI: 10.2503/hortj.qh-103

Rie Kurata, Yoshihiro Okada, Takeo Sakaigaichi, Yumi Kai, Akira Kobayashi, Keisuke Suematsu

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