N. Rajalakshmi, S. Sankar, Anu Bosewell, M. Mini, B. K. Mani, S. B
{"title":"Molecular characterization of the hypervariable regions of S1 gene of infectious bronchitis virus isolates","authors":"N. Rajalakshmi, S. Sankar, Anu Bosewell, M. Mini, B. K. Mani, S. B","doi":"10.56093/ijans.v94i2.137355","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.137355","url":null,"abstract":"A preliminary study was conducted for the detection of the infectious bronchitis virus circulating among the poultry flocks of Kerala employing a reverse transcriptase polymerase chain reaction, where, a high prevalence of the virus was reported. The present study is the first comprehensive study pertaining to the molecular characterization of hypervariable regions of the S1 gene of IBV in Kerala. The positive samples were sequenced with primers targeting the three hypervariable regions of the S1 subunit of spike gene. It was observed that the isolates of Kerala shared more than 95% similarity with isolates from different parts of India. On phylogenetic analysis of hypervariable regions 1 and 2, all the Kerala isolates clustered with isolates from other parts of the country. Phylogenetic analysis of the hypervariable region 3 revealed that the Kerala isolates branched separately from the isolates belonging to different parts of India. The study advocated the need of an area specific vaccine in Kerala, employing the potential candidate vaccine strain isolated in the study.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139842235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of intravenous xylazine versus dexmedetomidine premedication with ketamine-midazolam-isoflurane anaesthesia for castration in horses","authors":"Kavin Ravikumar, Sivashankar Ramakrishnan, Pushkinraj Haridoss, Kannan Thandavan Arthanari","doi":"10.56093/ijans.v94i2.135343","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.135343","url":null,"abstract":"The objectives of this research was to study the sedation and anaesthetic characteristics of intravenous xylazine and dexmedetomidine combined with ketamine-midazolam-isoflurane and to evaluate the cardiopulmonary effects during the anaesthetic period. Stallions were premedicated with xylazine (1.1 mg/kg IV) in Group 1 and dexmedetomidine (3.5 µg/kg IV) in Group 2. Sedation quality was evaluated and scored. General anaesthesia was achieved with ketamine (2.2 mg/kg IV) and midazolam (0.1 mg/kg IV) in both groups and anaesthesia was maintained with isoflurane inhalant in fresh oxygen gas flow (6-8 L/min). All the stallions underwent an orchiectomy by the half- closed method. Cardiovascular variables and pulmonary variables were recorded using multi gas vital sign monitor. Anaesthetic parameters, reflex status and quality of muscle relaxation were assessed. After the surgical procedure, recovery was monitored and scored on 6 point scale. Sedation score and quality was clinically better in Group 2. The difference in cardiopulmonary variables was statistically not significant. However, statistically significant changes were noticed between two groups with regard to some blood gas values during the intra-operative and at 15 min post anaesthetic period. Quality of muscle relaxation was better in group 2. Recovery was not significant. Overall quality of anaesthesia and recovery was almost similar in both the groups. In conclusion, both drug combinations produced satisfactory results for castration in the horses studied. In this study, dexmedetomidine at 3.5 µg/kg and xylazine at 1.1 mg/kg sedative doses did not result in much significant changes compared to one other.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"4 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139843286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Loya-Olguin, Y. Valdés-García, V. González-Vizcarra, H. Dávila-Ramos, J. D. Urías-Estrada, A. J. HERNÁNDEZ-CERÓN, J. G. RODRÍGUEZ-CARPENA, F. Grageola-Nuñez, A. Plascencia
{"title":"Handmade fish meal as a partial replacement of soybean meal in diets for feedlot lambs: Effects on growth performance, dietary energy and meat quality","authors":"J. Loya-Olguin, Y. Valdés-García, V. González-Vizcarra, H. Dávila-Ramos, J. D. Urías-Estrada, A. J. HERNÁNDEZ-CERÓN, J. G. RODRÍGUEZ-CARPENA, F. Grageola-Nuñez, A. Plascencia","doi":"10.56093/ijans.v94i2.136051","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.136051","url":null,"abstract":"With the aim to evaluate a handmade fishmeal (HFM) as a partial replacement of soybean meal (SBM) in finishing diets, 36 intact male Dorper × Pelibuey lambs (41.43±7.38 kg of initial weight) were used in a completely randomized block design to test the following treatments: 1) Cracked corn-based diet containing 12% SBM, 2) inclusion of 3.5% of HFM partially replacing SBM, and 3) inclusion of 7% of HFM partially replacing SBM. Urea and limestone were utilized to balance diets in CP and calcium content. The feeding trial lasted 30 days. Replacement of SBM with HFM did not modify the effects on average daily gain (ADG) and dry matter intake (DMI), but there were numerical differences in ADG; HFM inclusion linearly improved gain-to-feed ratio; dietary net energy (NE) and observed-to-expected diet NE. Hot carcass weight and dressing percentage were not affected by HFM. Except a linear increase on C22:6, the effect of SBM replacement on fatty acid profile in meat was not significant. The meat pH registered at 24 h post-mortem linearly increased with HFM inclusion, but meat colour and sensorial values were unaffected. It was concluded that inclusion of up to 7% of HFM in diet as partial replacement of soybean meal did not negatively affect DMI and ADG, but can increase feed efficiency and dietary energy utilization. The effects of HFM on carcass and meat quality were inappreciable. Due to variations in handmade processing, it is important to verify its chemical composition before HFM can be incorporated into diets.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139844286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Swayamprabha Naik, S. Dash, Jaspreet Singh Arora, P. P. Dubey, S. Sahoo
{"title":"Association of polymorphisms of IGF1 promoter with growth and fertility performance in PB1 parent line of broiler chicken variety","authors":"Swayamprabha Naik, S. Dash, Jaspreet Singh Arora, P. P. Dubey, S. Sahoo","doi":"10.56093/ijans.v94i2.133317","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.133317","url":null,"abstract":"Blood samples from 180 birds pertaining to a single generation of PB1 parent line were collected for present study. The birds were raised under standard management and different growth variables were recorded up to 20 weeks of age. Age at first egg and egg production till 40 weeks of age was recorded in females. PCR-RFLP analysis was used to screen individuals with polymorphisms in IGF1 promoter region and three genotypes AA, AC and CC were identified at frequencies of 0.79, 0.18 and 0.03, respectively. CC homozygotes were lower with respect to their performance in growth and fertility traits. Sequencing results of both alleles revealed T244G transversion mutation in the C allele. Gene regulation analysis confirmed that such transversion resulted in non-binding of Oct-1 transcription factor at 241 to 250 bp in C allele, causing down regulation of the gene. The mutations in the promoter sequence affected the transcriptional gene regulation affecting growth and fertility performance.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"1 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139782742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Sathyabama, T. A. Manivannan, K. Rajamanickam, A. Kirubakaran
{"title":"Oestrus induction and fertility response in post-partum anoestrus dairy cows treated with homeopathic medicine","authors":"T. Sathyabama, T. A. Manivannan, K. Rajamanickam, A. Kirubakaran","doi":"10.56093/ijans.v94.i2.131203","DOIUrl":"https://doi.org/10.56093/ijans.v94.i2.131203","url":null,"abstract":"The study was carried out to evaluate the effect of two homeopathic medicines to induce oestrus in 36 postpartum anoestrus crossbred Jersey dairy cows. Group 1 cows were fed a mineral mixture, and group 2 and 3 were medicated with Pulsatilla and Sepia drenched at the dose rate of 20 mL twice daily for 8 days, respectively. The mean plasma progesterone level (ng/mL) estimated using a radio immunoassay kit was significantly higher in group 2, followed by group 3 before and after medication on day 28 and 60, post artificial insemination (AI). Out of 12 induced for oestrus, 10 cows (83.33 %) and 9 cows (75.0 %) in group 2 and 3 were confirmed pregnant by rectal palpation on day 60 post AI, respectively. Both the homeopathic medicines showed 100% oestrus induction with a mean interval of 25.17 to 25.50 days, and Pulsatilla medicated cows showed improved fertility response than Sepia. The significantly higher level of progesterone after the withdrawal of homeopathic medication might be due to the release of circulating hormone, which blocks estradiol negative feedback. The subsequent increase reduces uterine PGF2 alpha secretion for a better conception rate.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"52 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139845053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. S N, B. Saikia, B. Konwar, H. Bayan, T. R. Devi, Champak Jyoti Das, H. Zorinpuii
{"title":"Comparative studies on haemato-biochemical changes following pre-emptive analgesia with tramadol, pentazocine lactate and meloxicam inpain management of canine ovariohysterectomy","authors":"C. S N, B. Saikia, B. Konwar, H. Bayan, T. R. Devi, Champak Jyoti Das, H. Zorinpuii","doi":"10.56093/ijans.v94i2.132408","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.132408","url":null,"abstract":"The present study was conducted to evaluate the effects of tramadol, pentazocine lactate and meloxicam as pre- emptive analgesics in dogs premedicated with glycopyrrolate, inducted with propofol and maintained with propofol continuous rate infusion (CRI) for certain haematological and biochemical parameters. The animals were randomly divided into three equal groups,viz. Group-T, Group-P and Group-M comprising six animals in each group and all the animals were premedicated with glycopyrrolate. After 10 min of pre-anaesthetic administration, pre-emptive analgesia was given. Blood was collected from cephalic or saphenous vein at intervals 0 (baseline) min before premedication, thereafter at 10 min, 30 min, 1 h, 2 h and 3 h after pre-emptive analgesic administration and haemato- biochemical parameters were recorded. Hb, PCV and TEC were significantly decreased at 30 min and 1 h interval in all the three groups. TLC and glucose concentration were significantly higher in group-M as compared to group-T and group-P at different time intervals. GGT level increased significantly at 30 min in all the three groups. CRP concentration was significantly higher in group-M as compared to group-T. Total protein was significantly decreased at 1 h interval in group-T and group-P, but in group-M such finding was noticed at 2 h interval. Cortisol was significantly lower in group-T in entire study period. The alterations in physiological and haematological parameters caused by tramadol, pentazocine lactate and meloxicam were found to be minimal and within the physiological limits. Tramadol produced less significant rise in CRP and cortisol concentrations which indicated better pain management. Based on the findings of the present study, it is concluded that tramadol is more effective as compared to pentazocine lactate and meloxicam in the management of post-operative pain due to canine ovariohysterectomy.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"20 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139783236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. R. Jawale, P. K. Bacche, S. Chopde, A. Dhok, S. Kawitkar, R. L. Werulakar, M. Kadam, M. Gole, N. V. Kurkure
{"title":"Phytosome-conjugated carvacrol: A novel approach for improving growth performance, intestinal morphology and economics of production in Broiler Chicken","authors":"M. R. Jawale, P. K. Bacche, S. Chopde, A. Dhok, S. Kawitkar, R. L. Werulakar, M. Kadam, M. Gole, N. V. Kurkure","doi":"10.56093/ijans.v94.i2.141294","DOIUrl":"https://doi.org/10.56093/ijans.v94.i2.141294","url":null,"abstract":"Essential oils are plant-derived aromatic volatile oils, and they contain bioactive compounds that have been shown to improve poultry nutrition. However, considering problems associated with the solubility and bioavailability of polyphenolic compounds, the study was planned to find out the effect of the novel feed-grade delivery system, phytosomes for conjugation of plant-derived polyphenolic compound carvacrol on the growth performance of broiler chickens. The experiment was conducted, on 240 broiler chicks for a period of 6 weeks. The chicks were divided into 4 groups having 4 replicates of 15 birds each. The birds in the control group (T0) offered a standard diet as per BIS (2007) specification. Group T1 received a standard diet supplemented with Bacitracin Methylene Disalicylate (BMD) antibiotic at standard dose and group T2 received a standard diet supplemented with carvacrol essential oil @100 mg/kg feed. Group T3 received a standard diet supplemented with phytosome-conjugated carvacrol essential oil (carvacrol @16.6%) @100 mg/kg feed. The performance of all the treatment groups was assessed with respect to the different performance parameters. The supplementation of phytosome-conjugated carvacrol essential oil (carvacrol @16.6%) @ 100 mg/kg feed was found beneficial in terms of growth performance, feed efficiency, and intestinal morphometry. In terms of economics of broiler production, the results revealed that the addition of phytosome- conjugated carvacrol essential oil and carvacrol essential oil in diets was found beneficial in reducing the cost of broiler production, thereby enhancing the margin of profit in broiler production and fetching higher net profit than the control group.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139782387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sahin Aktar Munshi, Abhijit Das, S. G P, M. C. A. Devi, S. Subash, S. Jeyakumar, M. Sivaram
{"title":"Socio-economic impact of COVID-19 pandemic on dairy farm households in West Bengal state","authors":"Sahin Aktar Munshi, Abhijit Das, S. G P, M. C. A. Devi, S. Subash, S. Jeyakumar, M. Sivaram","doi":"10.56093/ijans.v94i2.130041","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.130041","url":null,"abstract":"India is one of the leading economies that have been stuck hard by the COVID-19 pandemic and the stringent measures were put in place to combat it. Among several sectors, dairy sector is the most affected as dairy products are highly perishable and rely on time-sensitive supply chains. Though studies are available on the impact of COVID-19 pandemic on dairy sector, there are no studies on COVID-infected dairy farm households. The present study was an attempt to assess the socio-economic impact of COVID-19 pandemic on infected and uninfected dairy farm households in West Bengal. The study covered pre-lockdown, lockdown (both 1st and 2nd wave) and post-lockdown phases of COVID-19 pandemic. The primary data was collected from 150 dairy farm households (COVID-19 infected-75 and uninfected-75) in Murshidabad and Nadia districts of West Bengal. Dairy Economic Performance Index consisting of number of milch animals, milk yield, marketed milk, milk procurement price, concentrate price and veterinary cost was developed using principal component analysis. In order to make infected and uninfected groups statistically comparable, propensity score matching technique was employed. The index values were compared between matched infected and uninfected groups over different phases of COVID-19 pandemic. Dairy households incurred significant economic losses during the lockdown and post-lockdown periods due to increase in cost of concentrates, decline in the number of milch animals and drop in milk procurement prices. Dairy households faced constraints in procuring dry fodder, concentrate feed and in accessing veterinary care. COVID-19 infected dairy farm households had a greater socio-economic hurdle than that of uninfected households.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"46 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139784791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. P. Bansod, S. K. Saha, A. Verma, L. C. Chaudhary, T. M
{"title":"Effect of feeding cauliflower leaf meal on blood-biochemical, immunity status and carcass characteristics of growing goat kids","authors":"A. P. Bansod, S. K. Saha, A. Verma, L. C. Chaudhary, T. M","doi":"10.56093/ijans.v94i2.141644","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.141644","url":null,"abstract":"An experiment was conducted to evaluate the effect of feeding cauliflower leaf meal (CLM) on blood biochemical, immunity status and carcass characteristics of growing goat kids. Fifteen male non-descript goat kids of 6-8 months of age were divided equally into three groups (T0, T1 and T2). T0 group was fed with basal diet and T1 and T2 group were fed with 20 and 30% CLM in concentrate mixture for 120 days. The Hb, PCV, DLC, TLC, albumin, globulin, total protein, A:G ratio, Ca, P, Urea N and glucose levels were comparable among all groups of goat kids. However, total cholesterol was significantly lower in T1 and T2 group. The enzymatic profile including ALT, AST and ASP levels were comparable among all groups of kids. The serum antioxidants such as glutathione peroxidase and catalase were significantly higher in T2 group as compared to T1 and control group without affecting superoxide dismutase level. The thyroid hormones were non-significant among all groups of kids. The humoral and cell mediated immunity was improved in CLM fed group of kids. The carcass characteristics and meat composition were comparable among all groups of kids while TBAR value was lower in T2 group. Therefore, it may be concluded from the study that 20-30% CLM in concentrate mixture reduces the total cholesterol level, increases serum catalase and glutathione peroxidase level and improved keeping quality of meat, cell mediated and humoral immunity status of growing goat kids.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"312 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139843025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Rajalakshmi, S. Sankar, Anu Bosewell, M. Mini, B. K. Mani, S. B
{"title":"Molecular characterization of the hypervariable regions of S1 gene of infectious bronchitis virus isolates","authors":"N. Rajalakshmi, S. Sankar, Anu Bosewell, M. Mini, B. K. Mani, S. B","doi":"10.56093/ijans.v94i2.137355","DOIUrl":"https://doi.org/10.56093/ijans.v94i2.137355","url":null,"abstract":"A preliminary study was conducted for the detection of the infectious bronchitis virus circulating among the poultry flocks of Kerala employing a reverse transcriptase polymerase chain reaction, where, a high prevalence of the virus was reported. The present study is the first comprehensive study pertaining to the molecular characterization of hypervariable regions of the S1 gene of IBV in Kerala. The positive samples were sequenced with primers targeting the three hypervariable regions of the S1 subunit of spike gene. It was observed that the isolates of Kerala shared more than 95% similarity with isolates from different parts of India. On phylogenetic analysis of hypervariable regions 1 and 2, all the Kerala isolates clustered with isolates from other parts of the country. Phylogenetic analysis of the hypervariable region 3 revealed that the Kerala isolates branched separately from the isolates belonging to different parts of India. The study advocated the need of an area specific vaccine in Kerala, employing the potential candidate vaccine strain isolated in the study.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"54 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139782352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}