The Indian Journal of Animal Sciences最新文献_第4页

Estimation of genetic parameters as well as trends for growth traits in Harnali sheep 哈纳里绵羊生长特征遗传参数和趋势的估算

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.131099

Tarun Sehgal, S. Dahiya, D. Dalal, C. S. Patil, Poonam RATWAN1, Y. Bangar

{"title":"Estimation of genetic parameters as well as trends for growth traits in Harnali sheep","authors":"Tarun Sehgal, S. Dahiya, D. Dalal, C. S. Patil, Poonam RATWAN1, Y. Bangar","doi":"10.56093/ijans.v94i5.131099","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.131099","url":null,"abstract":"The current study focussed on the growth traits pertaining to Harnali sheep maintained at Sheep Farm, LUVAS, Hisar and data were recorded from pedigree sheets of 1278 animals. Growth traits considered were birth weight (BW), weaning weight (WW), six-month weight (6MW), yearling weight (YW), and pre-weaning average daily gain (PRWDG) and least-squares means for corresponding traits found to be 3.28±0.02 kg, 12.89±0.07 kg, 17.06±0.06 kg, 24.36±0.06 kg, and 106.74±0.61 g, respectively. Effect of different factors like period of birth, sex of animal and dams’ weight at lambing was studied on growth traits. Except YW, significant effect of period of birth was observed on all the growth traits. Sex had significant influence on BW only however, dams’ weight at lambing affected significantly all the considered growth traits except YW. By leveraging the sire component of variance and covariance from mixed model analysis, the paternal half-sib correlation method was used to estimate genetic as well as phenotypic parameters. Estimates of heritability for BW, WW, 6MW, YW and PRWDG were 0.28±0.07, 0.33±0.07, 0.31±0.07, 0.12±0.04 and 0.27±0.06, respectively. Genetic as well as phenotypic trends for WW and PRWDG were found to be positive. Moderate range of genetic variability at weaning weight and its positive genetic and phenotypic correlations with growth traits specially six month’s weight indicated that genetic improvement could be possible through early selection at weaning weight in Harnali sheep.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"77 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro antibacterial activity of Zingiber officinale (ginger) against bacteria isolated from reproductive tract of clinical endometritic Murrah buffaloes 姜对从临床子宫内膜炎穆拉水牛生殖道分离的细菌的体外抗菌活性

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.117256

Pushkar Sharma, Sushant Srivastava, R. Kant, Bhoopendra Singh, Saurabh

{"title":"In-vitro antibacterial activity of Zingiber officinale (ginger) against bacteria isolated from reproductive tract of clinical endometritic Murrah buffaloes","authors":"Pushkar Sharma, Sushant Srivastava, R. Kant, Bhoopendra Singh, Saurabh","doi":"10.56093/ijans.v94i5.117256","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.117256","url":null,"abstract":"Endometritis, an inflammation of the innermost uterine layer, is caused by a variety of bacteria, including Gram- positive and Gram-negative aerobes and anaerobes. This study aimed to identify the active compounds in Zingiber officinale (ginger) and assess its effectiveness against common bacterial pathogens found in Murrah buffaloes suffering from endometritis. The infrared spectrum of ginger powder displayed a range of wavelengths from 3361.89/cm to 664.26/cm, indicating the presence of eight functional groups. In the hydro-ethanolic ginger extract, the spectrum ranged from 3402.8/cm to 765.35/cm, revealing 12 functional groups. A total of 42 vaginal mucus samples were collected, with E. coli being the most prevalent in 23 samples, followed by S. aureus in 21 samples, Pseudomonas spp. in 12 samples, and Campylobacter spp. in 11 samples. To evaluate the in vitro antibacterial activity of ginger extracts, antimicrobial susceptibility tests were conducted at concentrations of 2.5, 5, 10, 20, 30, 40, and 50 mg/ml. Staphylococcus aureus exhibited the highest sensitivity to the ginger extract, while E. coli showed substantial resistance. In conclusion, ginger demonstrates potent antibacterial activity against multidrug-resistant uterine pathogens and could serve as an alternative therapy to mitigate the risk of drug resistance in treating uterine infections.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"31 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141029966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine circovirus 2d associated with stillborn mummified foetus of pig 与猪木乃伊死胎有关的猪圆环病毒 2d

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.144702

V. H B, T. Das, G. Saikumar

引用次数: 0

Surgical management of supracondylar femur fracture using rush pinning technique in dogs 在狗身上使用急针技术对股髁上骨折进行手术治疗

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.132420

Akshay Kumar, Amit Kumar, Yudhvir Rana, Sara Kaushal, Rohit Kumar, S. Tyagi, Adarsh Kumar, Ahan Nagpal

引用次数: 0

Development of one-step reverse transcription PCR assay for detection of porcine epidemic diarrhoea virus in pigs 开发用于检测猪流行性腹泻病毒的一步逆转录 PCR 分析法

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.131797

F. Singh, K. Rajukumar, D. Senthilkumar, G. Venkatesh, S. B. Sudhakar, Vijendra Pal Singh, Aniket Sanyal

{"title":"Development of one-step reverse transcription PCR assay for detection of porcine epidemic diarrhoea virus in pigs","authors":"F. Singh, K. Rajukumar, D. Senthilkumar, G. Venkatesh, S. B. Sudhakar, Vijendra Pal Singh, Aniket Sanyal","doi":"10.56093/ijans.v94i5.131797","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.131797","url":null,"abstract":"The present study aimed to develop an in-house one-step reverse transcription (RT) PCR assay as a diagnostic preparedness for the detection of porcine epidemic diarrhoea virus (PEDV) in pigs. Primers and gene construct targeting the nucleoprotein gene of PEDV were designed and synthesised. In vitro transcribed (IVT) RNA synthesised from linearised plasmid DNA containing the gene of interest was used as the positive control for the development of the RT-PCR assay. The RT-PCR protocol was optimised using different concentrations of molecular reagents, the gradient of annealing temperatures and other thermal cycling conditions. Analytical sensitivity of the RT-PCR assay was determined using 10-fold serial dilutions of the IVT-RNA directly and of the RNA extracted from swine faeces spiked with the IVT- RNA. The developed RT-PCR assay had analytical sensitivity of 939 and 2682 RNA copies at 10-7 and 10-6 dilutions in IVT-RNA directly and RNA extracted from spiked faeces, respectively. The RT-PCR assay was found to be specific for PEDV, without any amplification for classical swine fever virus, swine influenza virus, porcine reproductive and respiratory syndrome virus and transmissible gastroenteritis virus. All the known negative field faecal samples (n=126) of pigs tested negative by the developed RT-PCR. The one-step RT-PCR assay developed in the present study will be highly useful in specific diagnosis of the disease in the event of its future ingression, and will also aid in monitoring of PED in Indian swine population.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"6 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141054577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of feeding Moringa oleifera based complete pellet feed on milk yield, composition, fatty acids, somatic cell count and cell mediated immunityin lactating goats 饲喂以油辣木为基础的全价颗粒饲料对哺乳山羊的产奶量、成分、脂肪酸、体细胞数和细胞介导的免疫力的影响

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.147894

Ravindra Kumar, Shalini Vaswani, M. Arif

{"title":"Effect of feeding Moringa oleifera based complete pellet feed on milk yield, composition, fatty acids, somatic cell count and cell mediated immunityin lactating goats","authors":"Ravindra Kumar, Shalini Vaswani, M. Arif","doi":"10.56093/ijans.v94i5.147894","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.147894","url":null,"abstract":"Lactation cum feeding trial was conducted on 12 female Barbari goats (Age approx. 2-3 years and mean body weight 34.72 ±1.28 kg) for 120 days after approximately one month of kidding and effect on milk production, milk constituents, somatic cell count, milk fatty acid profile and cell mediated immunity was studied. Animals were divided into two groups (Gr G and Gr M) of six each as per completely randomized design. Two types of complete pellet (Moringa oleifera and Cicer arietinum) based was formulated containing sundried Moringa biomass and Gram straw along with conventional feed ingredients. Lactating goats of Gr G was fed with gram straw based complete pellet feed while goats of Gr M was fed with Moringa based complete pellet feed. Both the complete pellet feeds were iso nitrogenous. No significant difference in body weight changes was recorded between groups. The milk production (g/day) was higher (Avg. 14.54%) in Gr M as compared to Gr G at different fortnight. No significant difference was reported in the milk composition. Milk fat, SNF, Protein and lactose was similar in both the groups. Milk Somatic cell count (×10 3/ml) was significantly (P<0.05) lower in lactating goats fed with Moringa based feed. Cell-mediated immune response assessed through delayed-type hypersensitivity (DTH) reaction against phyto haemagglutinin (PHA-P) was more pronounced in Gr M at 72 and 96 hrs.Higher concentration of unsaturated fatty acids (about 16.19%) and lower saturated fatty acids (about 5.49%) was reported in Gr M. Milk fatty acid profile indicators like Index of atherogenicity (IA) was lower while hypo/hyper cholesteremic ratio and health promoting index was higher in milk of moringa based pellet fed goats. Present study concluded that Barbari goats fed with Moringa based complete pellet feed produced higher milk yield with healthier fatty acid profile with low somatic cell counts.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"273 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolactin gene (C576A) polymorphism is associated with milk production performance in crossbred Anglo-Nubian dairy goats 催乳素基因（C576A）多态性与杂交盎格鲁-努比亚奶山羊的产奶性能有关

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.139677

C. S. Moneva, P. Sangel, A. Angeles, M. Mendioro, Renato S A Vega

{"title":"Prolactin gene (C576A) polymorphism is associated with milk production performance in crossbred Anglo-Nubian dairy goats","authors":"C. S. Moneva, P. Sangel, A. Angeles, M. Mendioro, Renato S A Vega","doi":"10.56093/ijans.v94i5.139677","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.139677","url":null,"abstract":"Crossbred Anglo-Nubian goats (Anglo-Nubian × native goat breeds), are valued for exceptional milk production and adaptability. In-depth research on these goats is imperative to propel advancements in dairy goat breeds and enhance milk production efficiency within the region. The present study aims to assess polymorphism in prolactin (C576A) gene and determine the influence of different prolactin (PRL) genotypes to milk yield performance in crossbred Anglo-Nubian dairy goats raised from Awang, Opol, Misamis Oriental and Talay, Dumaguete City, Negros Oriental. Genomic DNA was extracted from hair follicles using commercial DNA extraction kit and PCR-RFLP was performed for the genotyping of the C576A polymorphism located in exon 5 of goats’ prolactin gene using Eco241 restriction enzyme. Genotypic frequencies of 0.56 for AA, 0.44 for AB, while allelic frequencies of 0.78 for A, and 0.22 for B were recorded. All populations followed the Hardy–Weinberg principle, except for dairy goats from Farm A located in Opol, Misamis Oriental. A two-way factorial (2 × 4) in a Randomized Complete Block Design was used to evaluate the relationship between genotypes and milk yield performance. AB genotype goats produced significantly higher milk yield traits (average daily milk yield and total milk production) than AA genotype, an indication that the polymorphism in the caprine PRL (C576A) gene influenced milk yield performance in the population of studied crossbred Anglo-Nubian goats. These results have to be validated in other dairy goat breeds.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"51 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141040946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR amplification, sequencing, and in silico analysis of holandric genes TSPYand SRY in Nili Ravi buffalo 尼利-拉维水牛全缘基因 TSPY 和 SRY 的 PCR 扩增、测序和硅学分析

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.123881

Sandeep Singh, Ranbir Singh, C. Mukhopadhyay

{"title":"PCR amplification, sequencing, and in silico analysis of holandric genes TSPYand SRY in Nili Ravi buffalo","authors":"Sandeep Singh, Ranbir Singh, C. Mukhopadhyay","doi":"10.56093/ijans.v94i5.123881","DOIUrl":"https://doi.org/10.56093/ijans.v94i5.123881","url":null,"abstract":"The present work has been designed to study the evolutionary perspectives of two important holandric genes, namely, testis-specific protein, Y-encoded (TSPY), and sex determining region, Y-encoded (SRY) in Nili Ravi buffalo. These Y-chromosomal genes are responsible for male sex development. The evolutionary aspects of these genes have not been studied in the Nili Ravi buffaloes from India. A study was carried out on the peripheral blood samples of three adult Nili Ravi bulls belonging to university farm. DNA was extracted using the phenol-chloroform isoamyl alcohol (PCI) technique and reported primer sequences were used to amplify the genes (partially) using a thermocycler. Three biological samples were analyzed using Sanger sequencing and were aligned using Clustal Omega. Separate phylogenetic trees for the genes were constructed using MEGA7 software. The selection pressure analysis and evolutionary divergence were individually studied for each of the genes. The current study reports the partial TSPY and SRY gene sequences of Indian Nili Ravi buffalo. Besides, the molecular evolution analysis indicates that the genes have experienced some purifying selection during the evolutionary process. Molecular characterization of these holandric genes (TSPY and SRY) in Nili Ravi will find applications in sex determination in Nili Ravi and other buffaloes.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"30 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141044176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jackfruit waste and peels: Potential as livestock feed 菠萝废料和果皮作为牲畜饲料的潜力

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.136138

S. Prusty, M. Gendley, A. K. Singh, M. Dubey, P. V. Patil, V. K. Sharma

引用次数: 0

Comparative evaluation of conventional and ultrasound assisted tube cystotomy for obstructive urolithiasis in cattle 传统和超声辅助管式膀胱切开术治疗牛梗阻性尿路结石的比较评估

The Indian Journal of Animal Sciences Pub Date : 2024-05-01 DOI: 10.56093/ijans.v94i5.135922

P. P. Sudrik, R. Suryawanshi, A. Ulemale, V. D. Aher, S. M. Agivale, V. S. Dhaigude

引用次数: 0