Anupam Datta, A. Baruah, A. Bora, D. Dutta, Jitendra Goswami, Deepak Bhuyan, Sukanta Das
{"title":"Exploring the impact of exogenous Kisspeptin-10 on ovarian follicular development and estrus behaviour in Lakhimi cows of Assam","authors":"Anupam Datta, A. Baruah, A. Bora, D. Dutta, Jitendra Goswami, Deepak Bhuyan, Sukanta Das","doi":"10.56093/ijans.v94i7.147401","DOIUrl":"https://doi.org/10.56093/ijans.v94i7.147401","url":null,"abstract":"The present study was carried out to assess the influence of exogenous intravenous kisspeptin-10 on the follicular dynamics, hormonal profile, and expression pattern of KiSS1 and KiSS1R of Lakhimi cows of Assam during the estrus period. Cows were examined every alternate day during the estrous cycle using a linear array ultrasound scanner and profiled in an ovarian map. The follicular wave patterns were mostly (66.7%) 2-wave cycle and 33.3% with 3-wave cycle in both treated and control group of cows. Kisspeptin-10 induced an early emergence of follicular waves with more follicular count and accelerated growth of dominant follicle in both 2 and 3 waves in treated group compared to the control. Significant augmentation of serum concentration of progesterone (10th day), LH (day 1) and estradiol (on the day before estrus) in treated cows was observed, however the hormonal dynamics were similar to other mammals Administration of Kisspeptin-10 has elicited a significant rise in the serum kisspeptin level with gradual increase of the transcripts encoding Kiss1 and Kiss1R genes (highest on 3rd day of estrous cycle) and declined gradually till day 11. Both the transcripts encoding Kiss1 and Kiss1R genes were gradually increased from day 17 and were higher two days before the beginning of the next estrous cycle. Intravenous administration of exogenous Kisspeptin-10 can be promising approach to augment the ovarian follicular dynamics in regards to early onset of wave, follicular numbers and diameter, to explore the optimum fertility in indigenous cows, Lakhimi.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"76 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141655466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Imane Mahari, Sara Ghaichat, Hasnae Zekhnini, F. El Mellouli, Hamid Lakhiari
{"title":"Comprehensive study of Capripoxviruses in Moroccan small ruminants: A RT-PCR analysis","authors":"Imane Mahari, Sara Ghaichat, Hasnae Zekhnini, F. El Mellouli, Hamid Lakhiari","doi":"10.56093/ijans.v94i7.147792","DOIUrl":"https://doi.org/10.56093/ijans.v94i7.147792","url":null,"abstract":"This study aimed to investigate and identify circulating Capripoxviruses in different regions of Morocco, using RT-PCR technique on various sample types. The results from the analysis of 980 samples lend considerable perceptions highlighting the diversity in outcomes across the tested samples. The analysis of Ct values revealed distinct patterns dependent on the nature of the sampled materials. Notably, scab samples exhibited lower Ct values indicating a higher concentration of viral load. Swab samples, in contrast, presented average Ct values signifying a moderate presence of viral DNA. Blood samples demonstrated Ct values within the range suggesting a lower viral load in the bloodstream. Furthermore, the study also revealed endemic Capripoxvirus outbreaks in Morocco, particularly in regions with a strong sheep breeding industry.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"134 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141656282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karam Chand, S. Subramaniam, S. Biswas, Siddharth Gautam, N. S. Kharayat, Gaurav Kumar Sharma
{"title":"Genetic and antigenic characterization of bluetongue virus serotype-1 isolated from goat in India","authors":"Karam Chand, S. Subramaniam, S. Biswas, Siddharth Gautam, N. S. Kharayat, Gaurav Kumar Sharma","doi":"10.56093/ijans.v94i6.144563","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.144563","url":null,"abstract":"Bluetongue virus (BTV) affects domestic and wild ruminants and is transmitted by Culicoides spp. In the present study, genetic and antigenic characterization of the BTV-1 serotype (MKD20/08/Ind isolate) isolated from goat was carried out to understand the phylogenetic relationship and molecular epidemiology of bluetongue. Full genome sequencing of BTV-1 isolate was carried out using next-generation sequencing (NGS). The sequence data was analyzed and the phylogenetic relationship of the goat isolate with other BTV-1 was established. Phylogenetic analysis grouped the MKD-20/08/Ind isolate within BTV serotype 1, with a high level of nucleotide sequence identity (>99%) to the previous BTV-1, confirming its serotype. The phylogenetic tree constructed from the outer capsid protein genes, seg-2 and seg-6, segregated MKD-20/08/Ind with other Indian BTV-1 isolates reported earlier. All BTV genome segments were found to be evolving under strong purifying (negative) selection. The mean substitution rate of different segments of BTV-1 isolates varied from 1.663×10-2 (seg-8) to 3.949×10-3 (seg-1) substitutions per site per year. The time to the most recent common ancestor (tMRCA) indicates all the genome segments of BTV-1 might have an older ancestor. The phenotypic antigenic relationship (r) of the MKD20/08/Ind isolate with other BTV-1 isolate-specific hyperimmune serum (HIS) determined by serum neutralization test (SNT) was 0.44 to 0.80. The neutralization behaviours of the MKD20/08/Ind with other BTV-1 isolate-specific HIS suggest probable minor subtype antigenic variation. Full-genome sequencing and phylogenetic analysis of BTV-1/MKD-20/08/Ind isolate indicate ancestral relationships amongst the Indian and Mediterranean BTV-1 isolates.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"49 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expression of recombinant classical swine fever virus E2 glycoprotein instable High Five cells","authors":"Ekta Bhardwaj, D. Deka, Ramneek Verma","doi":"10.56093/ijans.v94i6.145934","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.145934","url":null,"abstract":"Classical swine fever is one of the most important viral diseases of domestic pigs and wild boar. It is a notifiable disease to the World Organization for Animal Health. In the present study, codon optimized CSFV E2 gene was cloned into a laboratory-modified insect cell expression vector for producing the recombinant protein in secretory form. Endotoxin-free recombinant plasmid prepared from the positive clone was transfected into the High Five insect cell line and then selected against zeocin antibiotic for the development of a stable cell line. The stable High Five cell line successfully secreted E2 recombinant protein with a molecular weight of ~65 kDa as revealed by SDS-PAGE and Western blot. The recombinant E2 protein showed efficient reactivity with known CSFV anti-serum and monoclonal antibodies (MAbs against E2) in dot blot assay indicating its potential as a diagnostic antigen.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"38 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141346057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Chopade, Shrinivas Jahageerdar, B. Katkade, R. S. Deshmukh, M. Sawane
{"title":"Study of association of type traits with lifetime milk traits in Frieswal cattle","authors":"M. Chopade, Shrinivas Jahageerdar, B. Katkade, R. S. Deshmukh, M. Sawane","doi":"10.56093/ijans.v94i6.148640","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.148640","url":null,"abstract":"Most of the type traits have positive correlated response with lifetime traits. Therefore, these traits are important for selection of animals for high milk yield. Moreover, the udder depth can be used as a good indicator for predicting lifetime milk yield and length of productive life. Lastly, the selection may be supported with the detected QTLs, significant SNPs and candidate genes which are the potential tools to improve the lifetime traits.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"49 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Manoj, Sarin Kamboj, Rajesh Chhabra, Mahavir Singh
{"title":"Occurrence and characterization of multidrug-resistant extended spectrum β-lactamase producing Escherichia coli from bovine mastitis of Haryana","authors":"J. Manoj, Sarin Kamboj, Rajesh Chhabra, Mahavir Singh","doi":"10.56093/ijans.v94i6.150486","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.150486","url":null,"abstract":"A study was carried out to analyze 500 bovine milk samples collected from Haryana, India and 37% samples were found to be associated with E. coli as a causative agent for mastitis. Out of it, 53 (10.6%) isolates were found to be positive for ESBL production by Double-Disk Synergy test and Epsilon test. However, the ESBL-encoding gene blaCTX-M was detected in the 51 (10.2%) isolates. The antibiotic susceptibility test revealed 13.72% of the isolates were multidrug resistant, with MAR index varying from 0.06 to 0.46. The maximum sensitivity was observed towards gentamicin (90.19%) followed by chloramphenicol (78.4%). Fluoroquinolones antibiotics were found to be resistant between 84.31% to 76.47%. The resistance profile underscores the importance of antibiotic stewardship and the need for ongoing surveillance using one health approach to guide therapy and manage the spread of resistant bacteria.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"9 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141347560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kanika Tiwari, N. S. Jadon, Priyanka Pandey, M. Kandpal
{"title":"Clinico-physiological studies of atropine-tiletamine-zolazepam-sevofluraneanaesthesia with or without dexmedetomidine premedication in dogs","authors":"Kanika Tiwari, N. S. Jadon, Priyanka Pandey, M. Kandpal","doi":"10.56093/ijans.v94i6.135986","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.135986","url":null,"abstract":"The present study was conducted on twelve dogs randomly divided into two groups, viz. group A and B irrespective of age, breed and sex. Animals of group A were premedicated with atropine sulphate at the dose rate of 0.04 mg/kg body weight subcutaneously while in group B, atropine sulphate at the dose rate of 0.04 mg/kg body weight subcutaneously and dexmedetomidine at the dose rate of 5µg/kg body weight intravenously were administered at 5 min interval. Tiletamine-zolazepam was administered intravenously as induction agent and maintenance with sevoflurane in both the groups of animals. Clinico-physiological, haemodynamic and biochemical parameters were evaluated at various time intervals. Induction time was significantly lower whereas duration of anaesthesia, recovery time and complete recovery time were significantly higher in animals of group B as compared to group A. Excellent muscle relaxation and good analgesia was observed in the animals of group B. Abolition of pedal reflex and palpebral reflex was better in the animals of group B as compared to group A. Physiological parameters fluctuated within the normal limits. Significant decrease in PR interval and significant increase in QRS interval was recorded in the animals of group A. Non-significant changes were observed in the biochemical parameters except significant increase in the serum glucose level was observed at 30 min to 1 h in both the groups of animals. Thus, it was concluded that the anaesthetic combination of atropine-dexmedetomidine-tiletamine-zolazepam-sevoflurane produces better sedation, muscle relaxation and analgesia as compared to atropine-tiletamine-zolazepam-sevoflurane anaesthesia.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"52 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vikram Ramesh, L. S. Devi, Vivek Joshi, Sapunii Stephen Hanah, Kobu Khate, Meraj Haider Khan
{"title":"Cervical mucus changes can predict ovulation time in Mithun","authors":"Vikram Ramesh, L. S. Devi, Vivek Joshi, Sapunii Stephen Hanah, Kobu Khate, Meraj Haider Khan","doi":"10.56093/ijans.v94i6.134470","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.134470","url":null,"abstract":"The study aimed to assess the possibility of cervical mucus changes to predict ovulation time in Mithun. Experimental Mithuns (n=6) were estrus synchronized and cervical mucus was collected in different estrus phases from three consecutive estrous cycles (n=18). Cervical mucus colour, consistency, arborization pattern and cytology were evaluated. Plasma estradiol-17β (E2) and progesterone (P4) levels were determined during different phases of estrous cycles. Ovulation time and subsequent growth of the corpus luteum were determined using transrectal ultrasonography. Results indicated thick and moderate cloudy mucus with a typical arborization pattern in midestrus. Sticky caseous or white-coloured thick consistency cervical mucus was observed during metestrus in 66.66% (n=14) estrous cycles along with ovulation or ovulation occurring within the range of -3 to + 4.5 - +7.5 h after the appearance of white mucus. Cervical mucus cytology revealed percentages of polymorphonuclear neutrophils were significantly higher in metestrus. E2 concentrations reached the peak on the day of estrus and P4 concentrations were lower than 0.5 ng/mL. It was concluded that cervical mucus shows a typical arborization pattern in midestrus and the appearance of characteristic sticky caseous cervical mucus during metestrus can be used as a tool to predict ovulation time.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"71 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141348176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Suardana, Gusti Ayu Putu, Aristha Dewi, Iwan Harjono Utama
{"title":"Haematological changes to assess stress arising from different methods of slaughtering in pigs","authors":"I. Suardana, Gusti Ayu Putu, Aristha Dewi, Iwan Harjono Utama","doi":"10.56093/ijans.v94i6.147959","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.147959","url":null,"abstract":"This study aimed to investigate the blood profile and neutrophil and lymphocyte ratio (NLR) in pigs subjected to stunning and non-stunning. Forty pigs, aged between 6-8 months and weighing 80-100 kg, were used to assess haematological changes associated with different slaughter techniques and to evaluate stress levels. The samples were divided into two groups, the first group was subjected to stunning (with a captive bolt), and the other served as a non-stunning (control). Blood samples were collected from each pig using EDTA anticoagulant tubes, with 3 mL of blood collected per sample. These samples were then analyzed using an auto-haematology analyzer to measure various parameters including RBC, HB, PCV, MCV, MCH, WBC, neutrophils, lymphocytes, monocytes, and eosinophil, as well as the ratio of neutrophils and lymphocytes. The results showed that stunning pigs with a captive bolt exhibited significantly higher levels of RBC, PCV, and lymphocytes with a value of 8.144±1.66 (×106/µL); 41.5±13.62(%) and 41.805±7.82(%), compared to non-stunning pigs with a value of 7.209 ± 1.22 (×106/µL); 36.65±6.12(%); and 22.2±12.24 (%), respectively. However, stunning pigs exhibited lower WBC and neutrophils, measuring 13.825 ± 8.56 × 103/µL and 41.75±16.64(%) with captive bold, compared to non-stunning pigs with a value of 20.375±9.83 (×103/µL) and 63.30±19.04 (%) respectively. The NLR index was higher in non-stunning pigs, with a value of 2.851, compared to stunning pigs subjected to a captive bolt, with an index of 1.006. In conclusion, stunning with a captive bolt was found to reduce the stress level of pigs before slaughter, potentially leading to improvements in meat quality.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"52 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141347798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. W. Anizoba, N. Ikeh, C. Ezenwosu, F. Udeh, N. Machebe
{"title":"Effect of dietary organic selenium on growth performance, antioxidant status and carcass characteristics of broiler chicken reared in the tropics","authors":"N. W. Anizoba, N. Ikeh, C. Ezenwosu, F. Udeh, N. Machebe","doi":"10.56093/ijans.v94i6.144852","DOIUrl":"https://doi.org/10.56093/ijans.v94i6.144852","url":null,"abstract":"An experiment was conducted to determine the effect of dietary organic selenium inclusion on growth performance, antioxidant capacity, selenium retention and carcass characteristics in broiler chicken. Day-old broiler birds (160) were distributed randomly into four equal groups (T1, T2, T3 and T4) comprising of four replicated pens with ten birds in each. Different treatment groups of birds were designated as T1 (Basal diet without selenium-enriched yeast), T2 (Basal diet with 0.50 mg/kg selenium enriched yeast), T3 (Basal diet with 1.00 mg/kg selenium enriched yeast) and T4 (Basal diet with 1.50 mg/kg selenium enriched yeast) for 56 days. The results revealed that final body weight, weight gain and feed conversion ratio were significantly enhanced for birds in T2 at both stages than other groups. T4 group showed highest glutathione peroxidase, catalase, superoxide dismutase and selenium concentration with lower malondialdehyde concentration compared to birds receiving other treatments. Carcass traits such as live weight and dressed weight significantly improved in T2 whereas relative weights and lengths of organs increased as level of organic selenium increased. Therefore, it can be concluded that adding 1.50 mg/kg selenium yeast enhanced growth, oxidative status, selenium deposition, carcass and organ traits without having a negative impact on the birds’ physiological state.","PeriodicalId":512390,"journal":{"name":"The Indian Journal of Animal Sciences","volume":"21 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141346476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}