Cell Preservation Technology最新文献_第5页

Mammalian Cell Desiccation: Facing The Challenges 哺乳动物细胞干燥:面临挑战

Cell Preservation Technology Pub Date : 2006-12-01 DOI: 10.1089/CPT.2006.9996

Tamir Kanias, J. Acker

引用次数: 44

Embryonic Stem Cell Processing in Obtaining Insulin-Producing Cells: A Technical Review 胚胎干细胞加工获得胰岛素生成细胞:技术综述

Cell Preservation Technology Pub Date : 2006-12-01 DOI: 10.1089/CPT.2006.9997

R. Enseñat‐Waser, A. Santana, Beatriz Paredes, M. Zenke, J. Reig, E. Roche

引用次数: 5

In vitro culture of cryopreserved caprine ovarian tissue pieces and isolated follicles 超低温保存的山羊卵巢组织块和离体卵泡的体外培养

Cell Preservation Technology Pub Date : 2006-12-01 DOI: 10.1089/CPT.2006.9998

A. Rodrigues, S. Costa, Rita Santos, C. Amorim, C. M. Lucci, S. Báo, J. Nunes, D. Rondina, J. R. Figueiredo

{"title":"In vitro culture of cryopreserved caprine ovarian tissue pieces and isolated follicles","authors":"A. Rodrigues, S. Costa, Rita Santos, C. Amorim, C. M. Lucci, S. Báo, J. Nunes, D. Rondina, J. R. Figueiredo","doi":"10.1089/CPT.2006.9998","DOIUrl":"https://doi.org/10.1089/CPT.2006.9998","url":null,"abstract":"The objective of the present study was to evaluate the effects of cryopreservation of goat preantral follicles, in situ and isolated, using dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Follicles were cryopreserved and cultured in vitro, and follicle viability was assessed by Trypan Blue staining before and after culture. The viability of follicles cryopreserved in situ using 1.5 M DMSO (64% ± 5.3 and 60% ± 7.4) or 3.0 M EG (58% ± 3.7 and 45% ± 4.4) after days 1 and 5, respectively, of culture was lower (p 0.05). For isolated follicles, the percentage of viable fresh follicles was higher than follicles cryopreserved in EG on days 1 and 5 of culture, and in DMSO on day 1 of culture. Follicle diameter was not altered during culture, both for fresh and cryopreserved follicles and when follicles were cultured in situ or isolated. In conclusion, caprine preantral follicles were successfully cryopreserved, especially in situ, and follicle viability after isolation was similar between fresh and cryopreserved follicles after 1 day of culture, when DMSO was used. © Mary Ann Liebert, Inc.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"290-298"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.9998","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Effect of Quercetin on Capacitation Status and Lipid Peroxidation of Stallion Spermatozoa 槲皮素对种马精子获能状态及脂质过氧化的影响

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.169

M. Mcniven, G. Richardson

引用次数: 22

The Field of Cellular Cryopreservation 细胞冷冻保存领域

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.147

J. Baust

引用次数: 0

Effects of Osmotic Stress and Cryoprotectant Toxicity on Mouse Oocyte Fertilization and Subsequent Embryonic Development In Vitro 渗透应激和冷冻保护剂毒性对小鼠卵母细胞体外受精和胚胎发育的影响

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.149

J. Huang, Haixiao Chen, Seang Tan, R. Chian

引用次数: 29

Thermally Induced Introduction of Trehalose into Primary Rat Hepatocytes 热诱导海藻糖导入原代大鼠肝细胞

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.178

Xiaoming He, Arthi A. Amin, A. Fowler, M. Toner

引用次数: 22

Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation 用双参数Kedem-Katchalsky公式计算绵羊原始卵泡的渗透性

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.188

R. Devireddy, C. Amorim, S. Leibo

{"title":"Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation","authors":"R. Devireddy, C. Amorim, S. Leibo","doi":"10.1089/CPT.2006.4.188","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.188","url":null,"abstract":"The osmotic responses of isolated ovine primordial follicles when suspended in various concentrations of several cryoprotective additives (CPAs) have recently been published. We have used the Kedem-Katchalsky formulation, written with two parameters as variables, to calculate permeability of the follicle membrane to water, Lp, and permeability to CPAs, ω. By fitting the model to the osmotic responses of follicles determined by Amorim and colleagues, we were able to calculate the permeability coefficients in the presence of 0.5, 1.0, 1.5, 2.0, or 2.5 M solutions of each of the following CPAs: dimethylsulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH), and glycerol (GLY). In the presence of DMSO, the best-fit values of L p ranged from 0.15 to 0.29 × 10-14 m 3/N-sec, and ω ranged from 0.30 to 0.50 × 10 -11 mol/N-sec. In the presence of EG, the corresponding values of Lp ranged from 0.25 to 0.47 × 10-14 m 3/N-sec, and ω ranged from 0.20 to 0.65 × 10 -11 mol/N-sec. In the presence of PROH, Lp ranged from 0.10 to 0.62 × 10-14 m3/N-sec, and ω ranged from 0.10 to 0.40 × 10-11 mol/N-sec. And finally, in the presence of GLY, the best-fit values of Lp ranged from 0.19 to 0.32 × 10-14 m3/N-sec, and ω ranged from 0.05 to 0.32 × 10-11 mol/N-sec. The cross correlation or the reflection coefficient, σ, defined as σ = 1 - ωυCPA/ Lp, where υCPA is the partial mole volume of the CPA, ranged from 0.763 to 0.926 in the presence of DMSO, from 0.870 to 0.977 in the presence of EG, from 0.813 to 0.983 in the presence of PROH, and from 0.918 to 0.988 in the presence of GLY. These values of Lp and ω (and σ) can be used to predict the volumetric response of ovine follicles under arbitrary CPA loading and unloading conditions and consequently, the presumptive optimal conditions. © Mary Ann Liebert, Inc.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"188-198"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60913703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Therapeutic Use of Cord Blood 脐带血的治疗用途

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.161

W. Arcese, A. Picardi, R. Cerretti, L. Cudillo, G. Angelis, L. Franceschini, L. Felice, M. Postorino

引用次数: 5

A New Protection Solution for Freezing Small Numbers of Sperm Inside Empty Zona Pellucida: Osmangazi-Turk Solution 空透明带内冷冻少量精子的新保护液:Osmangazi-Turk溶液

Cell Preservation Technology Pub Date : 2006-11-17 DOI: 10.1089/CPT.2006.4.199

H. Hassa, Firdevs Gurer Dr., A. Yildirim, Cavit Can, V. Sahinturk, B. Tekin

引用次数: 5