Cell Preservation Technology最新文献

Effect of Liposome Charge and Composition on the Delivery of Trehalose into Red Blood Cells 脂质体电荷和组成对海藻糖进入红细胞的影响

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0008

J. Holovati, M. Gyongyossy-Issa, J. Acker

引用次数: 17

Artificial Collapse of Blastocysts Before Vitrification: Mechanical vs. Laser Technique and Effect on Survival, Cell Number, and Cell Death in Early and Expanded Blastocysts 玻璃化前囊胚的人工塌陷:机械与激光技术及其对早期囊胚存活、细胞数量和细胞死亡的影响

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0007

N. Desai, J. Szeptycki, M. Scott, F. Abdelhafez, J. Goldfarb

{"title":"Artificial Collapse of Blastocysts Before Vitrification: Mechanical vs. Laser Technique and Effect on Survival, Cell Number, and Cell Death in Early and Expanded Blastocysts","authors":"N. Desai, J. Szeptycki, M. Scott, F. Abdelhafez, J. Goldfarb","doi":"10.1089/CPT.2008.0007","DOIUrl":"https://doi.org/10.1089/CPT.2008.0007","url":null,"abstract":"The purpose of this study was to systematically examine blastocoelic fluid reduction prior to vitrification and its potential benefits. In addition, we compared artificial collapse (AC) by laser pulse to a mechanical method. Mouse and dicarded human blastocysts were used in this study. Blastocysts were collapsed using either a 10 ms pulse with a laser (LAC) or else mechanical puncture with a microneedle (MAC). Blastocysts were vitrified on cryoloops using a two-step ethylene glycol/dimethyl sulfoxide protocol. We examined the effects of AC on specific outcome parameters such as overall survival, reexpansion, cell proliferation, and DNA damage. Unlike others, we report overall high survival rates with expanded blastocysts even without fluid reduction. We did detect a significant increase in blastomeres showing signs of DNA damage in the control group (13%) in comparison to blastocysts AC prior to vitrification (LAC 3%, MAC 5%; p < 0.001). Control blastocysts exhibited a lower rate of reexpansion. Within 3 ...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 1","pages":"181-190"},"PeriodicalIF":0.0,"publicationDate":"2008-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2008.0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Tumor Grade Predicts Tumor Proportion in Fresh Frozen Tissue Samples for Breast Cancer Research 肿瘤分级预测乳腺癌研究中新鲜冷冻组织样本中的肿瘤比例

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0010

R. Gandour-Edwards, Sandra L. Taylor, L. Beckett

引用次数: 1

Glycine Protects Bile Physiology and Biliary-Specific Liver Cell Metabolism from Ischemia-Reperfusion Injury: A 1H NMR Study 甘氨酸保护胆汁生理和胆道特异性肝细胞代谢免受缺血再灌注损伤:一项1H NMR研究

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0006

T. Hafez, H. Sheth, G. Glantzounis, H. Parkes, A. Seifalian, B. Fuller, B. Davidson

{"title":"Glycine Protects Bile Physiology and Biliary-Specific Liver Cell Metabolism from Ischemia-Reperfusion Injury: A 1H NMR Study","authors":"T. Hafez, H. Sheth, G. Glantzounis, H. Parkes, A. Seifalian, B. Fuller, B. Davidson","doi":"10.1089/CPT.2008.0006","DOIUrl":"https://doi.org/10.1089/CPT.2008.0006","url":null,"abstract":"Liver ischemia-reperfusion (I/R) injury is a major complication of liver resection and transplantation. Cytokine release by activated Kupffer cells (KCs) play a central role in the inflammatory cascade of I/R injury. There is evidence that glycine may protect against liver I/R injury by inhibition of KC activity. However, its effect on bile flow, an established marker of hepatic function, and bile composition is not known. A rabbit model of hepatic lobar warm I/R was used. Under general anesthesia, the sham group (n = 6) underwent laparotomy alone for 7 h. The I/R group (n = 6) underwent 60 min of left and median lobe inflow occlusion and 6 h of reperfusion. The glycine + I/R group (n = 6) underwent a same procedure to controls after receiving glycine 5 mg/kg intravenous infusion for over 15 min. Bile flow was collected, measured, and analyzed by proton magnetic resonance spectroscopy. Glycine prevented the significant reduction in bile flow seen in I/R at 6 h reperfusion (145.0 ± 11.4 vs. 108.3 ± 28.2 μL...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 1","pages":"173-180"},"PeriodicalIF":0.0,"publicationDate":"2008-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2008.0006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Room Temperature Storage of Cultured Human Articular Chondrocytes 培养的人关节软骨细胞的室温贮藏

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0009

V. García, Eva García-Pérez, E. Belyakova, S. Llames, M. Pevida, F. Tévar, J. Otero, Á. Meana

引用次数: 2

Long-Term Preservation at Room Temperature of Freeze-Dried Human Tumor Samples Dedicated to Nucleic Acids Analyses 用于核酸分析的冻干人肿瘤样品的室温长期保存

Cell Preservation Technology Pub Date : 2008-11-12 DOI: 10.1089/CPT.2008.0003

C. Leboeuf, P. Ratajczak, Weili Zhao, L. Plassa, Magali Court, H. Pisonero, H. Murata, J. Cayuela, J. Ameisen, J. Garin, A. Janin

{"title":"Long-Term Preservation at Room Temperature of Freeze-Dried Human Tumor Samples Dedicated to Nucleic Acids Analyses","authors":"C. Leboeuf, P. Ratajczak, Weili Zhao, L. Plassa, Magali Court, H. Pisonero, H. Murata, J. Cayuela, J. Ameisen, J. Garin, A. Janin","doi":"10.1089/CPT.2008.0003","DOIUrl":"https://doi.org/10.1089/CPT.2008.0003","url":null,"abstract":"Tumor banks are the only means for researchers to study in situ human lesions in a reproducible manner. Large amounts of money are spent at national and international levels for proper collection, in respect of both quality control and ethics, most importantly for shared use of the human samples. Preservation as whole tissue, and not simply as extracted DNA or RNA, allows additional scientific use of the samples. However, economic issues influence long-term development of biobanks since low-temperature cryopreservation with long-term storage of numerous samples and transfers avoiding RNA degradation are particularly costly. We optimized freeze-drying protocols for tumor samples dedicated to molecular analyses in our biobank using directly snap-frozen samples as references. We then compared different long-term storage conditions for preservation of nucleic acids in tumor samples to corresponding normal tissues and human cell lines. When stored for 1 year at room temperature with dessicant and no light, cel...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 1","pages":"191-198"},"PeriodicalIF":0.0,"publicationDate":"2008-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2008.0003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Evaluation of Freeze Thaw Cycles on stored plasma in the Biobank of the Norwegian Mother and Child Cohort Study. 冻融循环对挪威母婴队列研究生物库中储存血浆的评价。

Cell Preservation Technology Pub Date : 2008-09-01 DOI: 10.1089/cpt.2008.0012

Liv Paltiel, Kjersti S Rønningen, Helle M Meltzer, Susan V Baker, Jane A Hoppin

{"title":"Evaluation of Freeze Thaw Cycles on stored plasma in the Biobank of the Norwegian Mother and Child Cohort Study.","authors":"Liv Paltiel, Kjersti S Rønningen, Helle M Meltzer, Susan V Baker, Jane A Hoppin","doi":"10.1089/cpt.2008.0012","DOIUrl":"https://doi.org/10.1089/cpt.2008.0012","url":null,"abstract":"BACKGROUND: In many cohort studies, biological specimens are being stored without specific plans for analyses. In the Norwegian Mother and Child Cohort Study biological specimens (DNA, plasma, and whole blood) are stored on 96-well plates and as a result may undergo multiple freeze-thaw cycles. METHODS: To explore the impact of multiple freeze-thaw cycles on chemical constituents, we conducted a quality control study using pooled EDTA-plasma. Over a two-year period, samples stored at -80 degrees C were subjected up to 100 freeze-thaw cycles. Specimens were analyzed in triplicate for sodium, cholesterol, triglycerides, vitamin E, aspartate aminotransferase (AST), and free fatty acids. We assessed the percent change of analyte concentration from the values for the first freeze-thaw cycle, because this is the baseline for all stored specimens. RESULTS: With the exception of free fatty acids, there was little change over the first 10 freeze-thaw cycles. A majority of analytes showed no significant changes until 30 freeze-thaw cycles. After 30 freeze-thaw cycles, the largest percent change was observed for free fatty acids (+32%), AST (+21%), and triglycerides (-19%). CONCLUSIONS: Human plasma can go through several freeze-thaw cycles before analysis without influencing sample integrity for the selected analytes.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 3","pages":"223-230"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/cpt.2008.0012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28951614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Quality Assurance in Tissue Resources Supporting Biomedical Research. 支持生物医学研究的组织资源质量保证。

Cell Preservation Technology Pub Date : 2008-07-30 DOI: 10.1089/cpt.2008.9993.

William E Grizzle, Katherine C Sexton, Walter C Bell

{"title":"Quality Assurance in Tissue Resources Supporting Biomedical Research.","authors":"William E Grizzle, Katherine C Sexton, Walter C Bell","doi":"10.1089/cpt.2008.9993.","DOIUrl":"https://doi.org/10.1089/cpt.2008.9993.","url":null,"abstract":"Modern biomedical research requires access to high quality specimens of human tissue with or without extensive clinical annotation. Multiple types of organizations have developed to supply human tissues to support biomedical research. These organizations follow different models including the specific models of 1) prospective collection, 2) tissue banking, and 3) tissue collection associated with clinical trials as well as the model of 4) a tissue resource that incorporates features of the other models. These types of organizations devoted to supplying tissues for research have chosen different goals to meet the different tissue and informational needs of the investigators to whom they supply tissue. In order to provide high quality tissues to support research, all models should rely on a strong quality assurance program with extensive quality control of the tissues being provided to support research. In addition to facilities which collect, process, store and provide tissues, the need for a rigorous QA program applies to all resources and infrastructures used to support biomedical research. The UAB Tissue Collection and Banking Facility which provides human tissue to support biomedical research has been functioning and developing since 1979. To our knowledge, similar programs in providing tissues from animals are less developed, but could easily follow the models which UAB and other institutions providing human tissues have established, including the approaches of UAB and others to QA and QC. This manuscript reviews the current concepts of QA and QC in use in organizations supplying tissue to support biomedical research as well as new approaches in QA and QC that have been proposed.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2008-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094015/pdf/nihms-116023.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29883228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Interactions of Trehalose-Containing Liposomes with Human Red Blood Cells 研究含海藻糖脂质体与人红细胞的相互作用

Cell Preservation Technology Pub Date : 2008-07-30 DOI: 10.1089/CPT.2008.0004

J. Holovati, M. Gyongyossy-Issa, J. Acker

引用次数: 11

Loading Trehalose into Red Blood Cells by Improved Hypotonic Method 改进低渗法将海藻糖装入红细胞

Cell Preservation Technology Pub Date : 2008-07-30 DOI: 10.1089/CPT.2008.0001

Xinli Zhou, Hui He, Baolin Liu, T. Hua

引用次数: 8