Glycine Protects Bile Physiology and Biliary-Specific Liver Cell Metabolism from Ischemia-Reperfusion Injury: A 1H NMR Study

Cell Preservation Technology Pub Date : 2008-11-12 DOI:10.1089/CPT.2008.0006

T. Hafez, H. Sheth, G. Glantzounis, H. Parkes, A. Seifalian, B. Fuller, B. Davidson

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引用次数: 3

Abstract

Liver ischemia-reperfusion (I/R) injury is a major complication of liver resection and transplantation. Cytokine release by activated Kupffer cells (KCs) play a central role in the inflammatory cascade of I/R injury. There is evidence that glycine may protect against liver I/R injury by inhibition of KC activity. However, its effect on bile flow, an established marker of hepatic function, and bile composition is not known. A rabbit model of hepatic lobar warm I/R was used. Under general anesthesia, the sham group (n = 6) underwent laparotomy alone for 7 h. The I/R group (n = 6) underwent 60 min of left and median lobe inflow occlusion and 6 h of reperfusion. The glycine + I/R group (n = 6) underwent a same procedure to controls after receiving glycine 5 mg/kg intravenous infusion for over 15 min. Bile flow was collected, measured, and analyzed by proton magnetic resonance spectroscopy. Glycine prevented the significant reduction in bile flow seen in I/R at 6 h reperfusion (145.0 ± 11.4 vs. 108.3 ± 28.2 μL...

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甘氨酸保护胆汁生理和胆道特异性肝细胞代谢免受缺血再灌注损伤:一项1H NMR研究

肝缺血再灌注(I/R)损伤是肝切除和移植的主要并发症。激活的库普弗细胞(KCs)释放细胞因子在I/R损伤的炎症级联反应中起核心作用。有证据表明甘氨酸可能通过抑制KC活性来保护肝脏I/R损伤。然而，其对胆汁流量(一种已确立的肝功能指标)和胆汁成分的影响尚不清楚。采用兔肝大叶热性I/R模型。假手术组(n = 6)在全麻下单独开腹7 h, I/R组(n = 6)左、中叶流闭塞60 min，再灌注6 h。甘氨酸+ I/R组(n = 6)在甘氨酸5 mg/kg静脉输注超过15分钟后，与对照组进行相同的程序。通过质子磁共振波谱法收集、测量和分析胆汁流量。甘氨酸阻止了再灌注6 h时I/R胆流量的显著减少(145.0±11.4比108.3±28.2 μL)。

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Cell Preservation Technology

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