Cell Preservation Technology最新文献_第4页

Protein Expression within the Human Renal Cortex and Renal Cell Carcinoma: The Implication of Cold Ischemia 人肾皮质蛋白表达与肾细胞癌:冷缺血的意义

Cell Preservation Technology Pub Date : 2007-08-01 DOI: 10.1089/CPT.2007.0001

H. Gudmundsdóttir, Fjola Haraldsdottir, Ásdís Baldursdóttir, R. Barkardottir, S. Hardarson, V. Gudnason, T. Gudbjartsson, G. Einarsson, E. Jonsson, V. Pétursdóttir

{"title":"Protein Expression within the Human Renal Cortex and Renal Cell Carcinoma: The Implication of Cold Ischemia","authors":"H. Gudmundsdóttir, Fjola Haraldsdottir, Ásdís Baldursdóttir, R. Barkardottir, S. Hardarson, V. Gudnason, T. Gudbjartsson, G. Einarsson, E. Jonsson, V. Pétursdóttir","doi":"10.1089/CPT.2007.0001","DOIUrl":"https://doi.org/10.1089/CPT.2007.0001","url":null,"abstract":"Cold ischemia of tissue during tissue treatment may influence protein expression, but has not been well studied. Better understanding of this is fundamental prior to using stored fresh-frozen tissue where the time from organ harvest until tissue collection and storage is most often not documented. We collected samples from normal renal cortex and cancerous tissues at serial time points for up to 60 min from three nephrectomized individuals with newly diagnosed clear cell renal cell carcinoma (RCC). Samples were processed onto protein chips and identified using surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI). The number and size of proteins expressed at separate sites within homogenous tissue sections were comparable. Cold ischemia time neither affected the number nor the size of proteins expressed. While the quantity of most proteins was similar between separate sites and unaffected by cold ischemia time, we noted variation in the quantity of some proteins compared to...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"5 1","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2007.0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60914739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Water Sorption and Glass Transition Behavior of Polyalditol (PD30), a New Nonreactive Malto-Oligosaccharide Cryo- and Drying-Protectant 新型非反应性麦芽寡糖冷冻干燥保护剂聚醛醇(PD30)的吸水和玻璃化转变行为

Cell Preservation Technology Pub Date : 2007-08-01 DOI: 10.1089/CPT.2007.0503

Wendell Q. Sun

引用次数: 1

Genomic Diversity Research and the Role of Biorepositories 基因组多样性研究和生物库的作用

Cell Preservation Technology Pub Date : 2007-08-01 DOI: 10.1089/CPT.2007.9993

Robert H. Hanner, T. R. Gregory

引用次数: 29

The Continuing Crisis in Biobanking 生物银行的持续危机

Cell Preservation Technology Pub Date : 2007-04-23 DOI: 10.1089/CPT.2007.9992

J. Baust

引用次数: 0

Comparison of a Selection of Rapid Automated DNA and RNA Extraction Technologies for Detection of Somatic or Constitutional Gene Abnormalities in Cancer Diagnosis 快速自动DNA和RNA提取技术在癌症诊断中检测体细胞或体质基因异常的比较

Cell Preservation Technology Pub Date : 2007-04-23 DOI: 10.1089/CPT.2006.9989

F. Lassailly, M. Mozziconacci, T. Noguchi, Didier Bechlian, J. Adélaı̈de, F. Fina, P. Martin, H. Sobol, L. Xerri, D. Birnbaum, C. Chabannon

{"title":"Comparison of a Selection of Rapid Automated DNA and RNA Extraction Technologies for Detection of Somatic or Constitutional Gene Abnormalities in Cancer Diagnosis","authors":"F. Lassailly, M. Mozziconacci, T. Noguchi, Didier Bechlian, J. Adélaı̈de, F. Fina, P. Martin, H. Sobol, L. Xerri, D. Birnbaum, C. Chabannon","doi":"10.1089/CPT.2006.9989","DOIUrl":"https://doi.org/10.1089/CPT.2006.9989","url":null,"abstract":"Molecular analyses of large numbers of patient samples are increasingly used for diagnostic applications as well as for understanding cancer biology. Their accuracy depends on the quality and quantity of nucleic acids extracted from human cells or tissues. To optimize these preanalytical steps, we evaluated several automated technologies for nucleic acid purification from clinical samples. Three automated platforms were compared. DNA was extracted from peripheral blood leukocytes from five normal individuals, and its quality was assessed by D-HPLC and sequencing after PCR. Clinical samples from acute leukemia patients were used for automated RNA extractions; results were compared to our standard manual technique. RNA qualification was done using capillary gel electrophoresis and analysis of the Abelson gene transcript by real-time PCR. One robot produced higher total output for both DNA and RNA. While the quality of DNAs obtained from the three workstations allowed implementation of their analysis for det...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"5 1","pages":"2-15"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.9989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60914367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Slow Freezing Versus Vitrification on the Recovery of Mouse Embryonic Stem Cells 慢速冷冻与玻璃化冷冻对小鼠胚胎干细胞恢复的影响

Cell Preservation Technology Pub Date : 2007-04-23 DOI: 10.1089/CPT.2006.9990

H. Miszta-Lane, P. Gill, M. Mirbolooki, J. Lakey

引用次数: 7

Progression of Cell and Tissue Vitrification 细胞和组织玻璃化的进展

Cell Preservation Technology Pub Date : 2007-04-23 DOI: 10.1089/CPT.2006.9988

J. Baust

引用次数: 0

A Modified Differential Scanning Calorimetry Method for Determining Water Transport Properties in Biological Cells During the Freezing Process 一种改进的差示扫描量热法测定生物细胞在冷冻过程中的水运输特性

Cell Preservation Technology Pub Date : 2007-04-23 DOI: 10.1089/CPT.2006.9991

Xu Han, D. Luo, X. Cui, S. Heimfeld, D. Gao

引用次数: 6

The Effect of Temperature Gradients on Stress Development During Cryopreservation via Vitrification. 温度梯度对玻璃化冷冻过程中应力发展的影响。

Cell Preservation Technology Pub Date : 2007-01-01 DOI: 10.1089/cpt.2007.9994

Paul S Steif, Matthew C Palastro, Yoed Rabin

{"title":"The Effect of Temperature Gradients on Stress Development During Cryopreservation via Vitrification.","authors":"Paul S Steif, Matthew C Palastro, Yoed Rabin","doi":"10.1089/cpt.2007.9994","DOIUrl":"https://doi.org/10.1089/cpt.2007.9994","url":null,"abstract":"<p><p>This study addresses the problem of thermal stress development in bulky specimens during cryopreservation via vitrification (vitreous means glassy in Latin). While this study is a part of an ongoing effort to associate the developing mechanical stress with the relevant physical properties of the cryopreserved media and to its the thermal history, the current paper focuses exclusively on the role of temperature gradients. Temperature gradients arise due to the high cooling rates necessary to facilitate vitrification; the resulting non-uniform temperature distribution leads to differential thermal strain, possibly resulting in cracking. The cooling rate is assumed constant on the outer surface in this study, and the material properties are assumed constant. It is demonstrated that under these assumptions, mechanical stress develops only when the temperature distribution in the specimen approaches thermal equilibrium at a cryogenic storage temperature. It is shown that the maximum possible stresses for a given cooling rate can be computed with a simple thermo-elastic analysis; these stresses are associated with cooling to sufficiently low temperatures and are independent of the variation of viscosity with temperature. Analytic estimates for these stresses are obtained for several idealized shapes, while finite element analysis is used to determine stresses for geometries used in cryopreservation practice. Stresses that develop under a wider range of storage temperatures are also studied with finite element analysis, and the results are summarized with suitable normalizations. It is found that no stresses arise if cooling ceases above the set-temperature, which defines the transition from viscous-dominated to elastic-dominated behavior; the set-temperature is determined principally by the dependency of viscosity upon temperature. Strategies for rapidly reaching low temperatures and avoiding high stresses are inferred from the results.</p>","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"5 2","pages":"104-115"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/cpt.2007.9994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27215112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

International Exchange of Cord Blood: Networks And Logistics 脐带血国际交流:网络和物流

Cell Preservation Technology Pub Date : 2006-12-01 DOI: 10.1089/CPT.2006.9995

J. Garcia, M. Torrabadella

引用次数: 1