Altex-Alternatives To Animal Experimentation最新文献_第6页

Trends in the use of animals and non-animal methods over the last 20 years. 过去 20 年中使用动物和非动物方法的趋势。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2410111

Katy Taylor

{"title":"Trends in the use of animals and non-animal methods over the last 20 years.","authors":"Katy Taylor","doi":"10.14573/altex.2410111","DOIUrl":"https://doi.org/10.14573/altex.2410111","url":null,"abstract":"Despite the importance of the animal testing issue, there has been little presentation in the scientific literature of the trends in animal use. This is crucial to resolve, particularly if we are to measure the impact of initiatives to reduce and replace animal experiments that were recently announced in Europe and the USA. For the first time, the number of animals used between 2002 and 2022 are presented for the EU, key animal-using countries in Europe (the UK, France and Germany), and North America (the USA and Canada). Animal testing is on a slow decrease in the EU, 11% in the last 20 years, but animal use in the UK, France and Germany is at similar levels as it was in 2002. Notably there has been a decrease in the production of genetically altered animals in the UK and a decrease in regulatory testing in the EU. Animal use in Canada has been steadily growing, and figures for the USA are still incomplete as laboratory-bred rodents and some other species are not counted. However, globally, the use of non-animal methods in biomedical research is increasing exponentially; this accelerated in the mid-2010s. The UK appears to be the leader in this field. The technological, regulatory, political and economic factors that might explain these trends are discussed.","PeriodicalId":51231,"journal":{"name":"Altex-Alternatives To Animal Experimentation","volume":"41 4","pages":"503-524"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive cell-based assay for testing potency of botulinum neurotoxin type A 测试 A 型肉毒杆菌神经毒素效力的灵敏细胞检测法。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 Epub Date: 2024-07-22 DOI: 10.14573/altex.2312071

Ceyda Caliskan, Deniz Simsek, Charlotte Leese, Ciara Doran, Elizabeth Seward, Andrew A Peden, Bazbek Davletov

{"title":"A sensitive cell-based assay for testing potency of botulinum neurotoxin type A","authors":"Ceyda Caliskan, Deniz Simsek, Charlotte Leese, Ciara Doran, Elizabeth Seward, Andrew A Peden, Bazbek Davletov","doi":"10.14573/altex.2312071","DOIUrl":"10.14573/altex.2312071","url":null,"abstract":"Botulinum neurotoxin type A (BoNT/A) is a biopharmaceutic widely used for the treatment of neurological diseases and in aesthetic medicine to achieve months-long paralysis of target muscles and glands. Large numbers of mice are used in the mouse bioassay (MBA) for various botulinum-related applications including batch release potency testing, antitoxin testing, countermeasure development, and basic research. BoNT/A intoxication causes severe suffering to the mice used for testing, and application-specific, non-animal alternatives are urgently needed. Cell-based assays (CBA) need to replicate all the physiological steps of botulinum intoxication, comprising neuronal binding, internalization, endosomal escape, and cleavage of synaptosomal-associated protein of 25 kDa (SNAP25). However, the CBA currently in use have limitations. In this study we show that LAN5 cells, a human neuroblastoma-derived cell line, are sensitive to BoNT/A and can be engineered to express a recombinant NanoLuciferase (NanoLuc)-tagged SNAP25 reporter molecule. On exposure, the reporter molecule is cleaved and releases a NanoLuc-SNAP25 fragment that can be captured on a 96-well plate for quantitative luminometry using a cleavage-specific SNAP25 antibody. We demonstrate, using purified BoNT/A and a commercial BoNT/A product, that the sensitivity of this new cell-based assay is in the fM range, comparable to that of the MBA. The new assay could replace the MBA in research and commercial testing of BoNT/A, benefiting both scientific progress and animal welfare.","PeriodicalId":51231,"journal":{"name":"Altex-Alternatives To Animal Experimentation","volume":" ","pages":"605-616"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No more monkey business: Advancing personalized medicine with animal-free research - Animalfree Research Forum 2023. 不再有猴子通过无动物研究推进个性化医疗 - 2023 年无动物研究论坛。

IF 5.6 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2311171

Miriam A Zemanova, Silvia Frey

引用次数: 0

Summer school for systematic reviews of animal studies: Fostering evidence-based and rigorous animal research. 动物研究系统综述暑期班：促进循证和严格的动物研究。

IF 5.6 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2310251

Marianna Rosso, Simona E Doneva, David W Howells, Cathalijn Hc Leenaars, Benjamin V Ineichen

引用次数: 0

Heads on! Designing a Qualification Framework for Organ-on-Chip. 迎头赶上！设计芯片上器官的资格认证框架。

IF 5.6 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 Epub Date: 2024-01-23 DOI: 10.14573/altex.2401231

Monica Piergiovanni, Milena Mennecozzi, Stavroula Sampani, Maurice Whelan

引用次数: 0

Non-animal models: Complexity for interactions…Connecting science. 非动物模型：相互作用的复杂性......连接科学。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2407181

Francesca Caloni, Alessandra Cazzaniga, Arno C Gutleb, Thomas Hartung, Helena Kandarova, Giulia Ranaldi, Hassan Rashidi, Doris Wilflingseder, Saliha Moutaharrik

引用次数: 0

Role of standards and funding in accelerating the development and use of microphysiological systems. 标准和资金在加速开发和使用微观生理系统方面的作用。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2407061

Surat Parvatam, Harshita Mittal, Francesca Pistollato, Kasturi Mahadik, Lorna Ewart, Abhijit Majumder, Y K Gupta, Sonja Beken, Rajeshwari Adheseshan, Glyn Stacey, Vishal Gandhi, Dhiraj Kumar, Helder Constantino, Goutami Nayak, Tejaswini Ghurde, Mousumi Gupta, Alokparna Sengupta, Troy Seidle

引用次数: 0

Corrigendum to Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment. 将新方法纳入监管性非临床药物安全性评估的更正。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2408291

Jan Turner, Pandora Pound, Carla Owen, Isobel Hutchinson, Marina Hop, David Y S Chau, Lady V Barrios Silva, Mike Coleman, Audrey Dubourg, Lorna W Harries, Victoria Hutter, J Gerry Kenna, Volker M Lauschke, Winfried Neuhaus, Clive Roper, Paul B Watkins, Jonathan Welch, Laura Rego Avarez, Katy Taylor

引用次数: 0

3 Days for 3Rs 2023: Refinement, reduction, replacement. 2023 年 3R 的 3 天：改进、减少、替代。

IF 5.6 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 DOI: 10.14573/altex.2402121

Augusto Vitale, Maurilio Calleri, Francesca Caloni, Cristina M Failla, Paola Granata, Laura Gribaldo, Urte Jaeh, Michela Kuan, Stefano Lorenzetti, Francesco Nevelli, Giulia Ranaldi, Irene Ruspantini, Orsola R Salva, Lena Smirnova, Julia Steitz, Luigia Trabace, Zoe Windsor, Isabella De Angelis

引用次数: 0

Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review. 污染物暴露和心肌损伤:毒理学系统制图审查的方案和进展报告。

IF 4.5 2区医学

Altex-Alternatives To Animal Experimentation Pub Date : 2024-01-01 Epub Date: 2023-11-20 DOI: 10.14573/altex.2304111

Tom Roos, Cathalijn Leenaars, Alexandra Schaffert, Martin Paparella, Sivakumar Murugadoss, Birgit Mertens, Nunzia Linzalone, Gabriele Donzelli, Merel Ritskes-Hoitinga, Ronette Gehring

{"title":"Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review.","authors":"Tom Roos, Cathalijn Leenaars, Alexandra Schaffert, Martin Paparella, Sivakumar Murugadoss, Birgit Mertens, Nunzia Linzalone, Gabriele Donzelli, Merel Ritskes-Hoitinga, Ronette Gehring","doi":"10.14573/altex.2304111","DOIUrl":"10.14573/altex.2304111","url":null,"abstract":"An increasing body of evidence identifies pollutant exposure as a risk factor for cardiovascular disease (CVD), while CVD incidence is rising steadily with the aging population. Although numerous experimental studies are now available, the mechanisms through which lifetime exposure to environmental pollutants can result in CVD are not fully understood. To comprehensively describe and understand the pathways through which pollutant exposure leads to cardiotoxicity, a systematic mapping review of the available toxicological evidence is needed. This protocol outlines a step-by-step framework for conducting this review. Using the National Toxicology Program (NTP) Health Assessment and Translation (HAT) approach for conducting toxicological systematic reviews, we selected 362 out of 8110 in vitro (17%), in vivo (67%), and combined (15%) studies for 129 potential cardiotoxic environmental pollutants, including heavy metals (29%), air pollutants (16%), pesticides (27%), and other chemicals (28%). The internal validity of included studies is being assessed with HAT and SYRCLE risk of bias tools. Tabular templates are being used to extract key study elements regarding study setup, methodology, techniques, and (qualitative and quantitative) outcomes. Subsequent synthesis will consist of an explorative meta-analysis of possible pollutant-related cardiotoxicity. Evidence maps and interactive knowledge graphs will illustrate evidence streams, cardiotoxic effects, and associated quality of evidence, helping researchers and regulators to efficiently identify pollutants of interest. The evidence will be integrated in novel adverse outcome pathways to facilitate regulatory acceptance of non-animal methods for cardiotoxicity testing. The current article describes the progress of the steps made in the systematic mapping review process.","PeriodicalId":51231,"journal":{"name":"Altex-Alternatives To Animal Experimentation","volume":" ","pages":"248-259"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0