Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)最新文献

{"title":"Translocator Protein TSPO (Peripheral Benzodiazepine Receptor): The Modern Story of the Ancient Preserved Protein with Ambiguous Functions","authors":"Yassir Mustafa Kamal Al Mulla Hummadi","doi":"10.31351/vol33iss1pp1-10","DOIUrl":"https://doi.org/10.31351/vol33iss1pp1-10","url":null,"abstract":"In several tissues, including the brain, heart, blood, intestines, adrenal glands, and liver, the 18 kDa translocator protein (TSPO) was shown to be the peripheral benzodiazepine receptor. There is strong evidence that TSPO is expressed in microglial cells in the central nervous system. Five transmembrane regions are seen at the cellular level in TSPO at the contact points between the outer and inner layers of mitochondria. The cytosolic region of the complex of amino acids that binds cholesterol is where cholesterol is taken up. TSPO is found as a monomer of 18 kDa and homomultimers and homodimers. Different factors, such as cholesterol concentration and reactive oxygen species, change the multimeric structure. As a result, TSPO gains responsibility for transferring cholesterol to the mitochondrial intermembrane space, transforming it into a steroid. Additionally, TSPO appears to collaborate with other mitochondrial membrane proteins to play a part in regulating the activity of the MPTP (mitochondrial permeability transition pore) and, therefore, in the elements of apoptosis. In vivo imaging of TSPO addresses a significant test in examining brain pathology like neuroinflammatory, Alzheimer’s, and schizophrenia. Additionally, TSPO’s use as a biomarker may have important implications for developing more viable diagnostic and therapeutic approaches. The current work surveys the TSPO cellular origin and attempts to comprehend its role in various physiological and pathological conditions.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":"101 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140379745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of FTIR Spectroscopy method for The Quantification of Ascorbic Acid in Bulk Materials and Pharmaceutical Formulation","authors":"Rihab Alhajjeh, Haifaa Al-Ali","doi":"10.31351/vol32iss3pp186-194","DOIUrl":"https://doi.org/10.31351/vol32iss3pp186-194","url":null,"abstract":"The present study illustrates the potential using of Fourier Transform Infrared Spectroscopy (FT-IR) in pharmaceutical analysis. A simple, rapid, non-destructive, and green (FT-IR) spectroscopy method for quality control evaluation of Ascorbic acid was developed, using potassium bromide (KBr) as a matrix to quantify the drug in bulk pharmaceutical materials and AUC its dosage forms. The sample preparation was done by mixing and grinding for disk composition and without using organic solvents. Absorbance obtained for the carbon-carbon band (C=C) located at the range (1600-1700) cm-1 was used to develop a calibration curve based on the AUC measurements. Method had been validated according to the International Conference on Harmonization (ICH) guidelines. Linearity, accuracy, precision, robustness, LOD and LOQ were evaluated and showed acceptable results for method validation in the concentration range of (2 - 12.5 mg). The validated method was able to apply for the quality control analysis of Ascorbic Acid raw materials and pharmaceutical dosage forms.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":"2 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of bioactive compounds from Juniperus oxycedrus L. plant grown in Iraq","authors":"Estabraq H. Naser, Sarmed H. Kathem","doi":"10.31351/vol32iss3pp172-185","DOIUrl":"https://doi.org/10.31351/vol32iss3pp172-185","url":null,"abstract":"Plant extracts are differ in quality and quantity of crud extract according to the extraction solvent, extraction time, and temperature. Herbal medicine used during the history for prevention and treatment of illnesses and promotion of health also used for enhancing the quality and span of our life. Juniperus oxycedrus banches and leaves are extracted with methanol using soxhlet apparatus to extract the polar compounds according to like dissolve like rule. The flavonoids components of type flavonol, as rutin, quercetin, and catechin, as well as flavone, as apigenin, were present in the crude extract that was extracted by preparative HPLC. The isolated compounds are examined using the UV spectrum, FT IR spectrum, mass spectrum by comparing the base peak mass m/z, and HPLC by comparing the retention times of the isolated and matching compounds.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":" 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139393571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cranberry on Outpatients with Mild to Moderate COVID-19 Infection","authors":"MR Atheer, Al-juhaishi, Munaf Aal-Aaboda, Amal U. Mosa","doi":"10.31351/vol32iss3pp165-171","DOIUrl":"https://doi.org/10.31351/vol32iss3pp165-171","url":null,"abstract":"COVID-19 disease is an acute viral infection illness of the airway system resulting from the SARS-CoV-2 virus. Cranberry is an evergreen group of dwarf shrubs of the Vaccinium Oxycoccus. The most important pharmacological effects of cranberry are related to its composition liking of quercetin, flavonols, and proanthocyanidins. cranberry had numerous pharmacological activities encompassing antioxidant, cardioprotective, antibacterial, anticancer, antiviral, and regulated glucose levels. This research aimed to estimate the potential activity of cranberry to enhance the efficacy of standard therapy for COVID-19 infection. A total of 30 patients with age ≥ 18 years suffering from mild to moderate COVID-19 pneumonia volunteered to do this study, allocated into two groups. The first group managed standard treatment only according to the National Institutes of Health, while the second group managed standard treatment plus cranberry 1000 mg capsule per day for 7 days. Each patient was assessed individually and follow up for 10 days. Cranberry significantly enhanced the effect of standard COVID – 19 therapies by rapidly relieving clinical symptoms of COVID – 19 infections and significantly lowered the count of WBC, CRP, D-Dimer, and ferritin in comparison with patients who only received STD therapy at P<0.05. In conclusion, Cranberry significantly improved STD therapy by reducing the inflammatory response and enhancing lung function via maintaining normal SPO2% level and relieving clinical symptoms of COVID-19 infection.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":"11 S9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139393917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular Endothelial Growth Factor Expression in Pulp Regeneration Treated By Hyaluronic Acid Gel in Rabbits","authors":"Rusul J. Hadi, Nawar B Kamil, N. AL-Ghaban, A. W. Izzat, يداه رفعج, لسر","doi":"10.31351/vol32iss3pp156-164","DOIUrl":"https://doi.org/10.31351/vol32iss3pp156-164","url":null,"abstract":"Among the most common dental problems, dental caries and trauma lead to the development of cavities and even tooth loss. Therefore, new regeneration techniques that are effective and toxic-free are needed. The use of scaffolds has created possibilities for the regeneration of tooth structure. Hydrogels made of hyaluronic acid have drawn many attention in regenerative medicine. Consequently, the objectives of the study were to estimate the impact of hyaluronic acid on pulp regeneration using histological evaluation and immunohistochemical localization of vascular endothelial growth factor (VEGF). The entire sample consisted of 20 teeth (right and left upper central incisors) from ten rabbits, Their ages were between 10 and 12 months. They were used for pulp exposure and coronal pulp tissue removal. After pulp exposure, hyaluronic acid hydrogels were injected into the pulp chambers of the right upper central incisors in the  experimental group to promote pulp regeneration. The pulp chambers of the left  upper central incisors considered as the control group, and they were temporarily filled in without the injection of hyaluronic acid. The histological and immunohistochemical evaluations were done in two time periods: the first and second week after pulp exposure. The histological results showed the formation of reparative dentin in the experimental group with a high mean value (56.3) regarding predentin thickness, especially in the second-week duration, and a significant difference as compared with the control group. The immunohistochemical results disclosed that the experimental group showed strong positive expression for vascular endothelial growth factor in different pulpal cells with the highest mean value of the number of positively expressed cells (34.3) in the second- week duration, with a significant difference as compared with the control group. Hyaluronic acid hydrogel had a supporting function in pulp regeneration, according to the findings of the current study. This was demonstrated by an increase in the expression of VEGF throughout the cells in pulp tissue.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":"31 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139394151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Docking, ADMET Study, Synthesis, Characterization and Preliminary Antiproliferative Activity of Potential Histone Deacetylase Inhibitors with Isoxazole as New Zinc Binding Group","authors":"Ali Mohammed Saeed, Ayad Abed Ali Al-Hamashi","doi":"10.31351/vol32isssuppl.pp188-203","DOIUrl":"https://doi.org/10.31351/vol32isssuppl.pp188-203","url":null,"abstract":"Histone acetylation is a highly interesting epigenetic target for drug therapy. Histone deacetylase enzymes (HDACs) are overexpressed in several diseases, including cancers. Most of the clinically used HDAC inhibitors involve hydroxamate group as a zinc-binding group (ZBG). Hydroxamates have a poor pharmacokinetic properties and high toxicity profile. Therefore, developing non-hydroximate HDAC inhibitors is a promising strategy for potency and selectivity enhancement. In this work, we designed new HDAC inhibitors with isoxazole moiety as ZBG using Ligand Designer from Glide (Schrodinger LLC). The cap group and the linker were optimized through trying various aliphatic and aromatic residues. The potential inhibition over HDAC8 for the optimally designed products was virtually evaluated using licensed Schrodinger modelling software. The results showed that the isoxazole has a potential bidentate interaction with HDAC8 active site zinc ion with acceptable fitness. ADMET/tox study performed to predict the pharmacokinetic properties for the final compounds. The final compounds showed a decent estimated drug-like properties. The intermediates and final compounds were successfully synthesized and purified using column chromatography. The chemical structure for intermediates and final compounds were characterized by IR and NMR spectroscopy.","PeriodicalId":512190,"journal":{"name":"Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)","volume":"32 Suppl.","pages":"188-203"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bijps.uobaghdad.edu.iq/index.php/bijps/article/download/1919/1219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}