Spectroscopy-An International Journal最新文献

A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies 钒酸盐、钒酸盐和十钒酸盐对肌动蛋白结构和功能影响的比较:几种光谱研究的结合

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/532904

S. Ramos, J. Moura, M. Aureliano

{"title":"A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies","authors":"S. Ramos, J. Moura, M. Aureliano","doi":"10.1155/2012/532904","DOIUrl":"https://doi.org/10.1155/2012/532904","url":null,"abstract":"The studies about the interaction of actin with vanadium are seldom. In the present paper the effects of vanadyl, vanadate, and decavanadate in the actin structure and function were compared. Decavanadate clearly interacts with actin, as shown by 51 V-NMR spectroscopy. Decavanadate interaction with actin induces protein cysteine oxidation and vanadyl formation, being both prevented by the natural ligand of the protein, ATP. Monomeric actin (G-actin) titration with vanadyl, as analysed by EPR spectroscopy, indicates a 1 : 1 binding stoichiometry and a kd of 7.5 μM. Both decavanadate and vanadyl inhibited G-actin polymerization into actin filaments (F-actin), with a IC50 of 68 and 300 μM, respectively, as analysed by light-scattering assays. However, only vanadyl induces G-actin intrinsic fluorescence quenching, which suggests the presence of vanadyl high-affinity actin-binding sites. Decavanadate increases (2.6-fold) actin hydrophobic surface, evaluated using the ANSA probe, whereas vanadyl decreases it (15%). Finally, both vanadium species increased e-ATP exchange rate (k = 6.5 × 10 �3 and 4.47 × 10 �3 s �1 for decavanadate and vanadyl, resp.). Putting it all together, it is suggested that actin, which is involved in many cellular processes, might be a potential target not only for decavanadate but above all for vanadyl.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"31 1","pages":"355-359"},"PeriodicalIF":0.0,"publicationDate":"2012-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86719129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

DCDR Spectroscopy as Efficient Tool for Liposome Studies: Aspect of Preparation Procedure Parameters ddr光谱学作为脂质体研究的有效工具:制备工艺参数方面

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/182720

E. Kočišová, A. Vodáková, M. Procházka

引用次数: 7

Impact of β-Turn Sequence on β-Hairpin Dynamics Studied with Infrared-Detected Temperature Jump 利用红外探测温度跳变研究β-转序对β-发夹动力学的影响

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/102423

Alexander Popp, Ling Wu, T. Keiderling, K. Hauser

引用次数: 5

Spectroscopic Determination of pKa Constants of MADS Box Segments MADS盒段pKa常数的光谱测定

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/674032

Barbora Řezáčová, Y. Coïc, C. Zentz, P. Turpin, J. Štěpánek

引用次数: 4

EPR and FTIR Spectroscopies Study of Human Blood after Irradiation 辐照后人体血液的EPR和FTIR光谱研究

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/365056

M. Polakovs, Ņ. Mironova-Ulmane, A. Pavlenko, E. Reinholds, M. Gavare, M. Grube, P. Stradins

引用次数: 6

The V30M Amyloidogenic Mutation Decreases the Rate of Refolding Kinetics of the Tetrameric Protein Transthyretin V30M淀粉样变性突变降低了四聚体转甲状腺素蛋白的再折叠动力学速率

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/502497

Catarina S. H. Jesus, Daniela C. Vaz, M. Saraiva, R. Brito

{"title":"The V30M Amyloidogenic Mutation Decreases the Rate of Refolding Kinetics of the Tetrameric Protein Transthyretin","authors":"Catarina S. H. Jesus, Daniela C. Vaz, M. Saraiva, R. Brito","doi":"10.1155/2012/502497","DOIUrl":"https://doi.org/10.1155/2012/502497","url":null,"abstract":"Transthyretin (TTR) is a homotetrameric protein implicated in several amyloid diseases. The mechanism by which TTR is converted into elongated fibrillar assemblies has been extensively investigated, and numerous studies showed that dissociation of the native tetrameric structure into partially unfolded monomeric species precedes amyloid formation. The small differences observed in the crystal structures of different TTR variants, as well as the thermodynamics and kinetics of tetramer dissociation, do not seem to completely justify the amyloidogenic potential of different TTR variants. With this in mind, we have studied the refolding kinetics of WT-TTR and its most common amyloidogenic variant V30M-TTR, monitoring changes in intrinsic tryptophan fluorescence at different urea and protein concentrations. Our results demonstrate that the in vitro refolding mechanisms of WT- and V30M-TTR are similar, involving a dimeric intermediate. However, there are large differences in the refolding rate constants for the two variants, specially close to physiological conditions. Interestingly, tetramer formation occurs at a much slower rate in the amyloidogenic variant V30M-TTR than in WT-TTR, which in the in vivo setting may promote the accumulation of monomeric species in the extracellular environment, resulting in higher susceptibility for aggregation and amyloid formation instead of spontaneous refolding.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"32 1","pages":"343-348"},"PeriodicalIF":0.0,"publicationDate":"2012-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81248114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Pt(II) Complexes with Linear Diamines—Part I: Vibrational Study of Pt-Diaminopropane Pt(II)与线性二胺配合物——第一部分:Pt-二氨基丙烷的振动研究

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/206297

Ana L. M. Batista de Carvalho, S. M. Fiuza, J. Tomkinson, Luís A. E. Batista de Carvalho, M. Paula M. Marques

引用次数: 11

Can Biofluids Metabolic Profiling Help to Improve Healthcare during Pregnancy 生物体液代谢分析有助于改善孕期保健吗

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/128367

G. Graça, Sílvia O. Diaz, J. Pinto, António S. Barros, I. Duarte, B. Goodfellow, E. Galhano, Cristina Pita, Maria do Céu Almeida, I. Carreira, Ana M. Gil

{"title":"Can Biofluids Metabolic Profiling Help to Improve Healthcare during Pregnancy","authors":"G. Graça, Sílvia O. Diaz, J. Pinto, António S. Barros, I. Duarte, B. Goodfellow, E. Galhano, Cristina Pita, Maria do Céu Almeida, I. Carreira, Ana M. Gil","doi":"10.1155/2012/128367","DOIUrl":"https://doi.org/10.1155/2012/128367","url":null,"abstract":"This paper describes a metabonomics study of 2nd trimester biofluids (amniotic fluid, maternal urine, and blood plasma), in an attempt to correlate biofluid metabolic changes with suspected/diagnosed fetal malformations (FM) and chromosomal disorders as well as with later occurring gestational diabetes mellitus (GDM), preterm delivery (PTD), and premature rupture of membranes (PROM). The global biochemical picture given by the threesome of biofluids should enable the definition of potential disease signatures and unveil potential metabolite markers for clinical use in predictive prenatal diagnostics. Results show that relatively strong metabolic disturbances accompany FM, reflected in all three biofluids and thus suggesting the involvement of both fetal and maternal metabolisms. Regarding GDM, amniotic fluid and maternal urine seem potential good media to detect early metabolic changes, and PTD subjects show small metabolite changes in the same biofluids, undergoing work being focused on plasma composition. Chromosomal disorders show an interestingly marked effect on maternal urine, whereas no statistically relevant early changes have been observed for PROM subjects. Interestingly, in the case of FM and chromosomal disorders, maternal biofluids show some sensitivity to disorder type, for example, for central nervous system malformations and trisomy 21, respectively. These results show the usefulness of biofluid metabonomics to probe overall metabolic disturbances in relation to prenatal disorders.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"21 1","pages":"515-523"},"PeriodicalIF":0.0,"publicationDate":"2012-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76518479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Spectroscopic Study of the Interaction of DNA with the Linker Histone H1 from Starfish Sperm Reveals Mechanisms of the Formation of Supercondensed Sperm Chromatin 海星精子中DNA与连接蛋白H1相互作用的光谱研究揭示了超浓缩精子染色质形成的机制

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/250489

E. Chikhirzhina, T. Starkova, Elena Kostyleva, A. Polyanichko

引用次数: 8

Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions 在可复制条件下培养的细菌种类和菌株的拉曼光谱

Spectroscopy-An International Journal Pub Date : 2012-07-11 DOI: 10.1155/2012/540490

J. Almarashi, N. Kapel, T. S. Wilkinson, H. Telle

引用次数: 35