Asia Pacific Journal of Molecular Biology and Biotechnology最新文献_第2页

Genomic surveillance and sequencing of SARS-CoV-2 in Malaysia 马来西亚 SARS-CoV-2 的基因组监测和测序

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-04-16 DOI: 10.35118/apjmbb.2024.032.2.09

Norazimah Tajudin, Seok Mui Wang, Darshan Chandra Kumar, Mariam Mohamad, S. F. A. Mohd. Nawi

{"title":"Genomic surveillance and sequencing of SARS-CoV-2 in Malaysia","authors":"Norazimah Tajudin, Seok Mui Wang, Darshan Chandra Kumar, Mariam Mohamad, S. F. A. Mohd. Nawi","doi":"10.35118/apjmbb.2024.032.2.09","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.09","url":null,"abstract":"This manuscript offers an in-depth review of the genomic surveillance of SARS-CoV-2 variants in Malaysia, emphasizing the integral role of this surveillance in understanding the virus's evolution and informing public health responses. Leveraging platforms like GISAID, Nextstrain, and the Pangolin classification system, researchers in Malaysia and their global counterparts share genome sequences and clinical data of SARS-CoV-2. These tools, particularly Nextstrain for real-time tracking and visualization of viral evolution, and Pangolin for lineage classification have advanced understanding of significant mutations, such as D614G and N501Y, and their impact on virus transmissibility and pathogenicity. The study of the virus's emergence in Malaysia offers vital insights into its evolutionary trajectories, aiding in effective pandemic management. Malaysia's Genomic Surveillance Program, aligned with national immunization efforts, plays a key role in identifying and controlling COVID-19 spread. This program integrates molecular, epidemiological, and clinical data that helps the health authorities in making a decision that leads to public health intervention and policymaking. This review details the significance of genomic surveillance in Malaysia, emphasizing its impact on understanding viral evolution, monitoring variants, informing public health strategies and responses, and preparing for future infectious disease challenges effectively.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"341 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140698221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Withania somnifera showed neuroprotective effect and increased longevity in Drosophila Alzheimer’s disease model 睡茄对果蝇阿尔茨海默病模型具有神经保护作用并能延长其寿命

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-04-16 DOI: 10.35118/apjmbb.2024.032.2.08

Mardani Abdul Halim, I. M. Rosli, Siti Shafika Muhamad Jaafar, Hui Min Ooi, Pui-Wei Leong, N. Rosli, S. Shamsuddin, N. Najimudin, G. Azzam

引用次数: 0

Review and bibliometric analysis of AI-driven advancements in healthcare 对人工智能推动的医疗保健进步进行回顾和文献计量分析

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-04-16 DOI: 10.35118/apjmbb.2024.032.2.10

Yi Jie Wang, W. Choo, K. Y. Ng

{"title":"Review and bibliometric analysis of AI-driven advancements in healthcare","authors":"Yi Jie Wang, W. Choo, K. Y. Ng","doi":"10.35118/apjmbb.2024.032.2.10","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.10","url":null,"abstract":"Purpose: This research intends to use literature review and bibliometric analysis methods to visually review the development status and important historical milestones of Artificial Intelligence, as well as the basic research, key topics, and future potential research hot spots of AI in the healthcare field. Methodology: Conduct in-depth analysis of AI in healthcare through bibliometrics methods such as publication activity analysis, co-occurrence analysis, and co-authorship analysis. Findings: This study outlines the development time trajectory of AI technology and its application in healthcare. Research shows that \"algorithm\", \"machine learning\", \"deep learning\", \"controlled study\", \"major clinical study\" and \"healthcare delivery\" as well as \"decision support systems\" are key topics for research. Gender-related research and ethical issues are areas of future focus. Research implications: The practical significance is that it can clarify and optimize the key directions of AI to improve the quality of medical decision-making, improve diagnostic accuracy and guide market investment. The originality is reflected in the comprehensive analysis of the development trajectory of AI in the medical and health field. Through a unique perspective and systematic approach, it provides an important reference for research trends and future directions in the field.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"136 3‐4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140698486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microplastics abundance in sea cucumber Holothuria scabra from Pulau Malawali, Sabah, Malaysia 马来西亚沙巴州马拉威利岛海参中的微塑料丰度

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.06

Sarah Syazwani Shukhairi, N. Mazlan, Nur Nashrah Abd Rahman, Muhammad Nor Afdall Nazahuddin, Amir Syazwan Shawel, A. D. Tuzan, Nurul Ain Jais, Vijay Kumar, S. N. Saud, Atna Permana

{"title":"Microplastics abundance in sea cucumber Holothuria scabra from Pulau Malawali, Sabah, Malaysia","authors":"Sarah Syazwani Shukhairi, N. Mazlan, Nur Nashrah Abd Rahman, Muhammad Nor Afdall Nazahuddin, Amir Syazwan Shawel, A. D. Tuzan, Nurul Ain Jais, Vijay Kumar, S. N. Saud, Atna Permana","doi":"10.35118/apjmbb.2024.032.2.06","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.06","url":null,"abstract":"The growing amount of plastic waste in the environment is a result of the increased use of plastics in numerous areas of our daily lives. These plastics are broken down into microplastics (MPs), which are 5 mm or smaller in size. Sea cucumbers are consumed as an exotic delicacy worldwide, however, it is suspected that they could ingest the microplastics due to their nature as filter feeder. In this study, we have identified the MPs presence in the intestines of sea cucumber Holothuria scabra. A total of 30 sea cucumber H. scabra were collected during low tide at a muddy sandy area at Pulau Malawali using bare hands. The gastrointestinal tracts of H. scabra were removed and digested in NaOH. MPs were identified using light microscope and categorized into shapes and colours. Micro-Fourier Transform-Infrared Spectroscopy (µFTIR) analysis was carried out to identify the types of polymers. The majority colours of MPs were black (69.84%) and blue (18.65%). Fibres (98.38%) made up the majority of MPs followed by fragments (1.37%) and films (0.25%). Ethylene Vinyl Acetate (EVA), Melamine-Formaldehyde (MF) and Rayon were among the polymers identified. In conclusion, MPs were found in the intestines of the sea cucumber H. scabra and this could be from the ingestion of polluted sea bed. The importance of studying the presence of these MPs in sea cucumber allows us to assess the impact of plastic pollution on marine animals and ecosystems and understand the potential risks to both sea cucumber and human as consumers.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"55 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140376532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper surface acts as good surface for biofilm attachment 铜表面是生物膜附着的良好表面

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.04

S. Rajan R

引用次数: 0

Morphological analysis, agronomic characteristics, species identification, and phylogenetic analysis of Pseuderanthemum sp. using the rbcLgene 利用 rbcL 基因对假花进行形态分析、农艺特征、物种鉴定和系统发育分析

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.05

Thieu Van Duong, Do Van Mai, Pham Thanh Trong, Tran Van Be Nam, Mai Van Hung

{"title":"Morphological analysis, agronomic characteristics, species identification, and phylogenetic analysis of Pseuderanthemum sp. using the rbcLgene","authors":"Thieu Van Duong, Do Van Mai, Pham Thanh Trong, Tran Van Be Nam, Mai Van Hung","doi":"10.35118/apjmbb.2024.032.2.05","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.05","url":null,"abstract":"The study aimed to evaluate the agronomic characteristics of eleven samples of Pseuderanthemum sp. variety based on the results of the morphological survey on stem height, root length, leaves, combining molecular biology methods to determine their genetic relationships by building a phylogenetic tree based on the plastid gene rbcL sequence. Initial results of an evaluation of the genetic diversity of eleven Pseuderanthemum sp. samples showed that there were differences in stem length, leaf width to root length. Based on the analysis results from the pedigree tree, the tree samples with similar survival indexes and genetically close to each other were classified into two main groups. Based on molecular data, eleven varieties of Pseuderanthemum sp. in the study have been identified, all belonging to the species Pseuderanthemum sp. have a high similarity coefficient above 98% and 100% coverage. Although the geographical location of the samples is variable, it is necessary to investigate other gene sequences related to morphological and biochemical characteristics, such as bioactive compounds in leaves and fruits to have more accurate conclusions about this species.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"14 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of CO2 absorption via terrestrial plants and microalgae: A review 比较陆生植物和微藻类对二氧化碳的吸收：综述

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.02

T. Erfianti, Istini Nurafifah, B. Sadewo, B. Daryono, E. Suyono, Arief Budiman

{"title":"Comparison of CO2 absorption via terrestrial plants and microalgae: A review","authors":"T. Erfianti, Istini Nurafifah, B. Sadewo, B. Daryono, E. Suyono, Arief Budiman","doi":"10.35118/apjmbb.2024.032.2.02","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.02","url":null,"abstract":"Currently, global warming and climate change continue to increase along with CO2 gas emissions. This has an impact on the survival of organisms, including humans. Therefore, efforts to reduce CO2 emissions have been conducted by various methods, such as chemical, physical and biological methods, one of the most efficient methods to absorb CO2 gas is to use microalgae. Microalgae are photosynthetic organisms capable of absorbing CO2. Microalgae can also be converted into valuable products such as biofuels, biofertilizers, food, feed, medicines, and cosmetics through an integrated biorefinery concept. In the future, CO2 mitigation using microalgae will be massively studied, considering the many benefits obtained from the utilization of microalgae to reduce CO2 emissions in the world. Through the concept of biorefinery, microalgae can be processed into various derivative products that are useful for humans in the food, feed, health, industrial, medicine, and cosmetic sectors. This review will compare the effectiveness of CO2 absorption through terrestrial plants, microalgae, and microalgae-bacterial consortia to the possibility of its application and challenges.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"34 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140377036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetraspanin CD9 peptides for membrane disruptive on P. aeruginosa 用于铜绿假单胞菌膜破坏的四蛋白 CD9 肽

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.03

Khairiyah Murad, S. Ab-Rahim, H. Al-Talib

引用次数: 0

Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation Komagataeibacter hansenii RM-03 以农业废弃物为底物的细菌纳米纤维素（BNC）生物合成及 BNC-银纳米复合材料制备方法

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-27 DOI: 10.35118/apjmbb.2024.032.2.01

Aini Darwina Daud, Nor’Aini Abdul Rahman, H. Foo, Rosfarizan Mohamad

{"title":"Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation","authors":"Aini Darwina Daud, Nor’Aini Abdul Rahman, H. Foo, Rosfarizan Mohamad","doi":"10.35118/apjmbb.2024.032.2.01","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.01","url":null,"abstract":"Bacterial nanocellulose (BNC) is a remarkable biopolymer synthesised by bacterium, exhibiting exceptional properties. However, conventional Hestrin-Schramm (HS) medium, particularly the carbon source, poses challenges of high costs and low productivity. This study explores BNC biosynthesis on a modified HS medium, employing agricultural wastes (sugarcane molasses, banana peel, and pineapple peel) as carbon sources, and compares the overall yield of BNC produced. Sugarcane molasses proved to be the most effective, yielding the highest BNC concentration (8.19 g/L) after 7 days, followed by pineapple peel (2.16 g/L) and banana peel (2.11 g/L). Extensive research was conducted to enhance properties of BNC by an environmentally friendly approach, incorporating silver nanoparticles (AgNP) utilising Momordica charantia fruit extract, resulting in a BNC-Ag nanocomposite. The synthesis involved mixing 1 mM silver nitrate (AgNO3) with 15 mL of M. charantia fruit extract to reduce Ag ions to AgNP, which was confirmed by UV-vis spectroscopy with an absorbance peak between 400 and 410 nm. Characterisation using FESEM and TEM on the synthesized BNC showed minimal impact on BNC fiber diameter from waste-derived carbon sources. XRD indicated slight variations in crystallinity index, with the highest (85%) in TSM-derived BNC. FTIR analysis revealed similar chemical profiles across all BNC, indicating cellulose formation. The BNC-Ag nanocomposite exhibited potent antibacterial activity against multi-drug resistant strains (Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtilis, Staphylococcus aureus) through disc diffusion method with inhibition zones up to 16.8 mm. Overall, the findings from this study contribute to the development of environmentally sustainable for the production of functional BNC materials with enhanced properties for diverse applications.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"16 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140375748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring actinobacteria isolated from coral originated from Tulamben Bali in inhibiting multidrug resistance bacteria 探索从巴厘岛图兰本珊瑚中分离的放线菌对多重耐药菌的抑制作用

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-03-19 DOI: 10.35118/apjmbb.2024.032.1.11

Fajar Hidayaturohman, A. Sabdaningsih, D. Ayuningrum

{"title":"Exploring actinobacteria isolated from coral originated from Tulamben Bali in inhibiting multidrug resistance bacteria","authors":"Fajar Hidayaturohman, A. Sabdaningsih, D. Ayuningrum","doi":"10.35118/apjmbb.2024.032.1.11","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.1.11","url":null,"abstract":"Increasing bacterial resistance has reduced the effectiveness of most clinical antibiotics. The limited option of antibiotics to treat Multi-Drug Resistance pathogen infections indicates that there is an urgency to explore new antibiotic candidates. Coral is recognized to produce bioactive compounds that have the potential to develop new medicinal materials with antibacterial properties. The use of marine invertebrates for compound production will reduce populations in nature, therefore associated microbes are preferred due to their environmental friendliness. Actinobacteria is one of the coral-associated microbes capable of producing active compounds of secondary metabolites as antibacterials. This study aimed to identify coral and obtain isolates of Actinobacteria associated with coral potential as an antibacterial against MDR bacteria. This research uses an explorative method. The method used is coral identification based on the morphology and shape of the sclerite. Screening of antibacterial activity was carried out using the agar plug method, microscopic observation using Gram staining and molecular identification of potential isolates by PCR. The results showed that the coral genera obtained were Siphonogorgia sp., Menella sp., Echinomuricea sp., Dendronephthya sp., and Epizoanthus sp. The results of antibacterial activity indicated that 19 isolates inhibited the growth of MDR pathogen bacteria. However, only the potential isolates were identified. The microscopic observations showed isolate III.ISP2.10-2.3 and III.ISP2.10-2.6 have Gram-positive coccus and bacillus-shaped, respectively. Potential isolates III.ISP2.10-2.3 (Micrococcus yunnanensis) were found to have antibacterial activity against Acinetobacter baumanii, Pseudomonas aeruginosa, and Bacillus subtilis, while isolate III.ISP2.10-2.6 (Brevibacterium pigmentatum) had the greatest activity against B. subtilis.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"156 9‐12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0