Asia Pacific Journal of Molecular Biology and Biotechnology最新文献

Immunomodulatory activity of mahanimbine from Murraya koenigii (Curry Leaf) on lipopolysaccharide-induced RAW 264.7 macrophages 咖喱叶中的马钱子碱对脂多糖诱导的 RAW 264.7 巨噬细胞的免疫调节活性

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-07-04 DOI: 10.35118/apjmbb.2024.032.3.03

Nor Malia Abd Warif, Nur Vaizura Mohamad, V. Mariappan

{"title":"Immunomodulatory activity of mahanimbine from Murraya koenigii (Curry Leaf) on lipopolysaccharide-induced RAW 264.7 macrophages","authors":"Nor Malia Abd Warif, Nur Vaizura Mohamad, V. Mariappan","doi":"10.35118/apjmbb.2024.032.3.03","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.3.03","url":null,"abstract":"One of the kinds of carbazole alkaloids isolated from Murraya koenigii leaves is mahanimbine. Although this substance has the potential to be an immunotherapeutic agent such as immunostimulatory, antibacterial, antiviral and anticancer, however, its immunomodulatory effects have not yet been completely characterised. Mahanimbine's immunomodulatory effects on RAW 264.7 macrophages induced by lipopolysaccharide (LPS) were investigated in this research. Mahanimbine was applied to the RAW 264.7 macrophage cells for 24 hours, and the cell viability was assessed using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) test. Using an ELISA detection assay, the production of IL-1β and TNF-α by stimulated RAW 264.7 cells was quantified. The substance had no cytotoxicity or effect on cell viability at any of the measured concentrations. In addition, mahanimbine did not demonstrate any changes in nitrite oxide production (14.06-15.40 μM) and IL-1β (251.0–238 pg/ml). However, the compound significantly enhanced the TNF-α production (344.4-426.0 pg/ml). The findings provide evidence of the immunomodulatory potential of mahanimbine by regulating pro-inflammatory cytokines.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141677934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of vitamins and minerals with COVID-19: A mini review 维生素和矿物质与 COVID-19 的关系：微型综述

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-07-04 DOI: 10.35118/apjmbb.2024.032.3.02

Khaldoon Jasim Mohammed, Ahmed Falah Imran, Salam Kitab Rubat

引用次数: 0

Enhancing the performance of Methylorubrum extorquens AAZ-1 by using CRISPR/Cas9 for the degradation of hydrocarbon compounds 利用CRISPR/Cas9提高Methylorubrum extorquens AAZ-1降解碳氢化合物的性能

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-07-04 DOI: 10.35118/apjmbb.2024.032.3.05

A. Maki, Asaad M. R. Al-Taee, Zeenah Atwan

{"title":"Enhancing the performance of Methylorubrum extorquens AAZ-1 by using CRISPR/Cas9 for the degradation of hydrocarbon compounds","authors":"A. Maki, Asaad M. R. Al-Taee, Zeenah Atwan","doi":"10.35118/apjmbb.2024.032.3.05","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.3.05","url":null,"abstract":"An efficient expression method was used to create a genetic modification strategy for methylotrophic bacteria to improve the capacity of bioremediating bacteria to absorb oil in contaminated environments. Using the plasmid-treated CRISPR-Cas9 system, Methylorubrum extorquens AAZ-1(OR226417.1) was treated, and qPCR analysis revealed that the expression of the MxaF gene (encoding methanol dehydrogenase enzyme) rose six-fold. To increase the biodegradation effectiveness of hydrocarbons (n-alkane and PAH), CRISPR-Cas9 technology was selected for the investigation and use in the laboratory. The biodegradation rate of n-alkane in the modified M. extorquens AAZ-1 rose from 61.14% to 74.35% during a seven-day incubation period when compared to the control. The proportion of polycyclic aromatic hydrocarbons in the altered M. extorquens AAZ-1 rose from 65.69% to 78.23%. To the best of the authors’ knowledge, this work is the first time that a CRISPR-Cas9 system has been employed to improve the efficiency of these bacteria in the biodegradation of hydrocarbon molecules.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141679900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of mangrove bark tannins and copper ions on marine bacteria 红树皮单宁酸和铜离子对海洋细菌的抗菌活性

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-07-04 DOI: 10.35118/apjmbb.2024.032.3.04

Sharifah Radziah Mat Nor, D. Ibrahim, Mohd Jain Kassim, Siti Aisyah Daud, N. Salikin, Syarifah Ab Rashid

{"title":"Antimicrobial activity of mangrove bark tannins and copper ions on marine bacteria","authors":"Sharifah Radziah Mat Nor, D. Ibrahim, Mohd Jain Kassim, Siti Aisyah Daud, N. Salikin, Syarifah Ab Rashid","doi":"10.35118/apjmbb.2024.032.3.04","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.3.04","url":null,"abstract":"A novel sealant or antifouling agent is desperately needed since numerous antifouling biocides, including tributyltin and diuron, have been banned due to their hazardous effects on the marine environment. Mixed-tannin extracted from Rhizophora apiculata was selected as the natural source to be combined with copper ions to form a copper-tannate (Cu-T) complex. The Cu-T complex was tested for antimicrobial properties via disc diffusion assay against a series of identified marine fouling bacteria. The zone of inhibition obtained from the effect of Cu-T on test bacteria ranged from 10.3 mm-21.0 mm. Meanwhile, the range for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) varied from 0.25 mg/mL-1.00 mg/mL and 0.50 mg/mL-2.00 mg/mL, respectively. The Cu-T complex affected the growth of eight marine fouling bacteria, with bacteriostatic effect on Bacillus aquimaris IBRL FB13 and Vibrio alginolyticus IBRL FB6. Time kill assay which was performed at the extract concentrations of 1/2MIC, MIC and 2MIC revealed that the antibacterial activity of Cu-T complex was concentration-dependent. From the photomicrographic observation, the Cu-T complex deteriorated the cell walls of the Gram-positive and Gram-negative bacteria. This study highlights the potential of the Cu-T complex as a sealant for mitigating biofouling formation on aquaculture equipment.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141679238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of V59L and A953G genotypes distribution in AQP7 and their association with glycerol in overweight/obese Malay patients 鉴定马来超重/肥胖患者 AQP7 中 V59L 和 A953G 基因型的分布及其与甘油的关系

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-07-04 DOI: 10.35118/apjmbb.2024.032.3.01

Mardhiah Masri, Ruzi Hamimi Razali, T. A. Abdul Rahman, Fathimah Mohamad, Xin Wee Chen, Aletza Mohd Ismail, R. Abdul Ghani, Rose Adzrianee Adnan, A. Azizi

{"title":"Identification of V59L and A953G genotypes distribution in AQP7 and their association with glycerol in overweight/obese Malay patients","authors":"Mardhiah Masri, Ruzi Hamimi Razali, T. A. Abdul Rahman, Fathimah Mohamad, Xin Wee Chen, Aletza Mohd Ismail, R. Abdul Ghani, Rose Adzrianee Adnan, A. Azizi","doi":"10.35118/apjmbb.2024.032.3.01","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.3.01","url":null,"abstract":"One significant public health issue contributing to cardiovascular risk factors is obesity. Previous evidence suggested that abnormal glycerol metabolism and aquaporin 7 (AQP7) dysfunction in promoting glycerol influx and efflux from the adipose tissue are among the mechanisms involved in obesity. This pilot study aims to identify the genotype distribution of polymorphisms in the AQP7 gene (AQP7) and to find their association with plasma glycerol. A cross-sectional study was undertaken at Hospital Universiti Teknologi MARA (HUiTM) Sungai Buloh, Selangor, Malaysia, on 56 normal and 44 overweight/obese participants. Anthropometry data was collected from all participants. Blood samples were taken by venipuncture to measure plasma glycerol and subsequently, the genotypes of two SNPs in AQP7 (V59L rs4008659 and A953G rs2989924) were determined for both groups. The genotype distribution and allele frequencies of both SNPs in the AQP7 were established, and their association with plasma glycerol was estimated by logistic regression. Participants in the overweight/obese group had higher plasma glycerol (median = 0.78 mg/dL, Q1-Q3=0.47-1.42) than the normal group. V59L and A953G genotypes distribution between normal and overweight/obese groups showed no significant difference. Logistic regression analysis showed that participants with the A953G (rs2989924) TC genotype had a 71% decreased risk of developing abnormal plasma glycerol when factors such as age, gender, and waist-to-hip ratio (WHR) were controlled. No similar association was seen in the participants with the V59L (rs4008659) genotypes. This study highlighted the potential role of the A953G (rs2989924) TC genotype in reducing the risk of having impaired glycerol metabolism, reflecting its likely protective nature against obesity.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141678702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Heat Shock Protein 70 in thyroid gland tumors 甲状腺肿瘤中热休克蛋白 70 的表达

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-05-08 DOI: 10.35118/apjmbb.2024.032.2.15

Haider A. Hassan, Saad Hasan Mohammed Ali, A. Al-hijazi

{"title":"Expression of Heat Shock Protein 70 in thyroid gland tumors","authors":"Haider A. Hassan, Saad Hasan Mohammed Ali, A. Al-hijazi","doi":"10.35118/apjmbb.2024.032.2.15","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.15","url":null,"abstract":"Heat shock protein 70 (HSP70) is a crucial protein with vital biological tasks in cell continuation of life. The variation of HSP70 activation occurs as a consequence of stress that includes temperature states, toxicity, poisoning with heavy metals, and tumor-related conditions. One of the master jobs of the HSP family is the suppression of caspase-mediated apoptosis signals. A high level of the expression of HSP70 is accountable for tumorigenesis and resistance against chemotherapeutic drugs. For this reason, the detection of HSP70 may help to diagnose cancerous diseases. From the other side, targeting this chaperone might help in treatment by maintaining late caspase-dependent events. This study was conducted to detect the presence and the location of HSP70 in Iraqi thyroid tumor tissue specimens (25 samples), in addition to 10 samples of normal thyroid tissue. Using the immunohistochemical study (paraffin method), the protein was detected in 100% of follicular carcinoma or follicular adenoma (benign) in addition to 77.7 % of papillary thyroid carcinoma while, in normal thyroid tissue, the presence of protein was in 10 % of cases. Regarding protein location in the cells, it appeared in the nuclei and the cytoplasm of follicular carcinoma cases in comparison with just in the cytoplasm of other sections.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140998439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrofurazone biodegradation kinetics by batch fermentation of Aspergillus tamarii 塔马曲霉批量发酵的硝基呋喃酮生物降解动力学

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-05-08 DOI: 10.35118/apjmbb.2024.032.2.11

Nur Aisyah Mohamad, Muhammad Zafri Zamri, M. N. Saat, Z. Ariffin

{"title":"Nitrofurazone biodegradation kinetics by batch fermentation of Aspergillus tamarii","authors":"Nur Aisyah Mohamad, Muhammad Zafri Zamri, M. N. Saat, Z. Ariffin","doi":"10.35118/apjmbb.2024.032.2.11","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.11","url":null,"abstract":"Nitrofurazone (NFZ) compound contains a 5-nitrofuran ring structure that has been widely used as feed additives in animal husbandry. Due to the benzene ring structure, the residues are highly toxic to humans and animals. A kinetics study of NFZ biodegradation in batch fermentation with Aspergillus tamarii KX610719.1 was conducted. The main objectives were to determine the kinetic parameters of fungal growth, glucose consumption, protein production, and biodegradation of NFZ using fungal biomass. Kinetic parameters were determined using Polymath 6.0 software, and regression analysis was done using linear and non-linear methods. After 168 hours of batch fermentation, the maximum specific growth rate (µmax), and maximum cell concentration (Xmax) for cultivation without NFZ were 0.062 h-1, and 0.529 g L-1, respectively. The maximum specific growth rate (µmax), and maximum cell concentration (Xmax) for cultivation with NFZ were 0.092 h-1, and 0.327 g L-1, respectively. For glucose consumption, kinetic parameters of Yield of biomass over the substrate (YXS) and cell maintenance (mS) were estimated at 0.139 g g-1 and 0.239 h-1, respectively. Based on the Luedeking Piret model, the estimated growth-associated (α) and non-growth-associated (β) constants were 1.142×10-2 g g-1 and 5.680×10-5 h-1, respectively. The rate constant (k1) of NFZ biodegradation was estimated at 2.696×10-2 h-1 following the first-order model where the rate constant of NFZ removal is dependent on the NFZ concentration. The application of A. tamarii batch fermentation in the removal of NFZ compound was sufficient with a total percentage removal of 85.9 % or 0.430 g L-1 recorded.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140998725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of biomimetic HAp formation on graphite 石墨上生物仿生 HAp 形成的研究

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-05-08 DOI: 10.35118/apjmbb.2024.032.2.12

R. Khaleel, M. S. Hashim, Murthada Kutheir Abbas

引用次数: 0

Effect of storage condition on the viability of sago effluents as carbon source in fermentation medium for bioethanol production 储存条件对西米废水作为生物乙醇生产发酵培养基碳源的可行性的影响

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-05-08 DOI: 10.35118/apjmbb.2024.032.2.13

Mohamad Zulhisyam Rashid, Dyg Salwani Awg Adeni, Muhammad Norhelmi Ahmad

{"title":"Effect of storage condition on the viability of sago effluents as carbon source in fermentation medium for bioethanol production","authors":"Mohamad Zulhisyam Rashid, Dyg Salwani Awg Adeni, Muhammad Norhelmi Ahmad","doi":"10.35118/apjmbb.2024.032.2.13","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.2.13","url":null,"abstract":"In Sarawak, Malaysia, approximately 237 tons/day of sago effluent is commonly discharged into nearby river due to the sago starch extraction process. Due to the high concentration of polymeric compounds, particularly starch, in sago wastewater, which petrifies easily, this condition severely pollutes the environment in the affected area. This study was conducted to determine the viability of using sago effluent as a carbon source and fermentation medium for bioethanol production which indirectly help to minimize the environmental impact as well as the economics of the sago industry. The sago effluent obtained from the local sago mill was analysed for starch content and pH profile while stored at room and cold (4°C) temperature facility. Enzymatic hydrolysis was conducted to convert the residual starch into glucose as carbon source for bioethanol fermentation. Fresh sago effluent can be stored for up to 5 days in cold temperature where the starch content remains constant. The highest starch concentration in sago effluent was 61.33 g/L, in which 50.57 g/L glucose was obtained through the enzymatic hydrolysis process. Hence 82.5% of the starch to glucose conversion yield is revealed. Then, the sago effluent hydrolysate which acts as a carbon source as well as a fermentation medium able to generate 23.14 g/L of bioethanol, displays a 91% theoretical yield of glucose to ethanol. In conclusion, the utilization of sago wastewater as feasible alternative to cheap and locally available and sustainable source of raw materials to produce bioethanol.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":" 35","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141000981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antibacterial potential of tunicate-associated bacteria (Ascidiacea) at the shipwreck site of Menjangan Kecil Waters, Karimunjawa 探索卡里蒙查瓦（Karimunjawa）Menjangan Kecil 水域沉船遗址中与鳞茎有关的细菌（Ascidiacea）的抗菌潜力

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-05-08 DOI: 10.35118/apjmbb.2024.032.2.14

Aen Hendrawati, D. Ayuningrum, A. Sabdaningsih, Rosa Amalia

引用次数: 0