Asia Pacific Journal of Molecular Biology and Biotechnology最新文献

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Morphological, cytotoxic and apoptotic effects of different concentrations of nisin ZP on MG63 cells in a 3-dimensional culture system 不同浓度的尼生素 ZP 在三维培养系统中对 MG63 细胞的形态学、细胞毒性和凋亡效应

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-02-09 DOI: 10.35118/apjmbb.2024.032.1.02

Alyaa R. Alkhateeb, S. Ab-Rahim, Muhammad F. Azmi, Aisha M. Din, Effat Omar, Gabriele Ruth Anisah Frömming

{"title":"Morphological, cytotoxic and apoptotic effects of different concentrations of nisin ZP on MG63 cells in a 3-dimensional culture system","authors":"Alyaa R. Alkhateeb, S. Ab-Rahim, Muhammad F. Azmi, Aisha M. Din, Effat Omar, Gabriele Ruth Anisah Frömming","doi":"10.35118/apjmbb.2024.032.1.02","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.1.02","url":null,"abstract":"Osteosarcoma (OS) is a primary bone cancer that commonly affects young individuals. Despite treatment, it is known to be highly resistant to therapy. Nisin, a bacteriocin released by Lactococcus lactis has been shown to exhibit an apoptotic effect on cancer cells. To better represent in vivo cancer cell constructs, 3-dimensional (3D) culture systems have been used in vitro. This study aims to compare the effects of nisin on OS cell culture in both monolayered and 3D gas-permeable VECELL® G-Plate. Osteosarcoma cells (MG63) were cultured on 3D culture (G-Plate) and normal culture plates. Morphological assessments were carried out through light and scanning electron microscopic examinations. The results showed that the use of a 3D system is more reliable in the development of 3D osteosarcoma models compared to the 2-dimensional (2D) culture system. Specifically, the 3D culture demonstrated the formation of MG63 spheroids, indicating the reliability of the hanging drop methods to produce spheroidal cell morphology. Additionally, the cell viability assay showed a higher IC50 value for 2D groups compared to the 3D groups, with values of 102.56 µg/mL and 33.96 µg/mL, respectively. Finally, a dose-dependent apoptosis was noticed for the apoptotic assay, further demonstrating the effectiveness of the 3D culture system. The effectiveness of nisin ZP as an anticancer agent was observed in both 2D and 3D culture systems. However, the 3D culture system required a higher dose of nisin ZP to demonstrate its potential, indicating that this system could be a promising approach in OS intervention studies.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"163 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139849796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the extracellular secretion of black goat rumen metagenome-derived KG42 xylanase by Bacillus subtilis 优化枯草芽孢杆菌对源自黑山羊瘤胃元基因组的 KG42 木聚糖酶的胞外分泌

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-02-02 DOI: 10.35118/apjmbb.2024.032.1.01

Ji-Eun Kim, Jin-Sung Lee, Donghwan Lee, Hoyoon Choi, Keun-Sung Kim

{"title":"Optimization of the extracellular secretion of black goat rumen metagenome-derived KG42 xylanase by Bacillus subtilis","authors":"Ji-Eun Kim, Jin-Sung Lee, Donghwan Lee, Hoyoon Choi, Keun-Sung Kim","doi":"10.35118/apjmbb.2024.032.1.01","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.1.01","url":null,"abstract":"Xylanase (E.C. 3.2.1.8) is the enzyme that breaks down β-1,4 xylan by cleaving β-1,4 glycosidic linkages. Production of xylanases is important for various industrial applications. Here, we aimed to determine the optimal incubation conditions for expression and secretion of KG42 xylanase in Bacillus subtilis using response surface methodology based on Box-Behnken design in preparation for industrial applications. Among nine broth media tested in this study, Power Broth was chosen as a basal medium. In addition to the basal medium, the four other independent variables of extra carbon sources (glucose, lactose, mannose, fructose, and sucrose), extra nitrogen sources (beef extract, yeast extract, tryptone, urea, NaNO3, and (NH4)2SO4), isopropyl β-D-1-thiogalactopyranoside concentrations, and induction times were individually tested using one factor at a time in an optimization experiment. Next, a Box-Behnken design-based response surface methodology approach was used to identify and validate the optimized incubation conditions with the four variables in batch culture. The statistically optimized incubation conditions obtained from this study yielded a maximum of approximately 3- to 4-fold increases in the expression and secretion of KG42 xylanase by B. subtilis in comparison with unoptimized medium and incubation conditions.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"111 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139810176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asia Pacific Journal of Molecular Biology and Biotechnology Pub Date : 2024-02-02 DOI: 10.35118/apjmbb.2024.032.1.01

Ji-Eun Kim, Jin-Sung Lee, Donghwan Lee, Hoyoon Choi, Keun-Sung Kim

{"title":"Optimization of the extracellular secretion of black goat rumen metagenome-derived KG42 xylanase by Bacillus subtilis","authors":"Ji-Eun Kim, Jin-Sung Lee, Donghwan Lee, Hoyoon Choi, Keun-Sung Kim","doi":"10.35118/apjmbb.2024.032.1.01","DOIUrl":"https://doi.org/10.35118/apjmbb.2024.032.1.01","url":null,"abstract":"Xylanase (E.C. 3.2.1.8) is the enzyme that breaks down β-1,4 xylan by cleaving β-1,4 glycosidic linkages. Production of xylanases is important for various industrial applications. Here, we aimed to determine the optimal incubation conditions for expression and secretion of KG42 xylanase in Bacillus subtilis using response surface methodology based on Box-Behnken design in preparation for industrial applications. Among nine broth media tested in this study, Power Broth was chosen as a basal medium. In addition to the basal medium, the four other independent variables of extra carbon sources (glucose, lactose, mannose, fructose, and sucrose), extra nitrogen sources (beef extract, yeast extract, tryptone, urea, NaNO3, and (NH4)2SO4), isopropyl β-D-1-thiogalactopyranoside concentrations, and induction times were individually tested using one factor at a time in an optimization experiment. Next, a Box-Behnken design-based response surface methodology approach was used to identify and validate the optimized incubation conditions with the four variables in batch culture. The statistically optimized incubation conditions obtained from this study yielded a maximum of approximately 3- to 4-fold increases in the expression and secretion of KG42 xylanase by B. subtilis in comparison with unoptimized medium and incubation conditions.","PeriodicalId":511206,"journal":{"name":"Asia Pacific Journal of Molecular Biology and Biotechnology","volume":"59 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139870078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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