Journal of Steroid Biochemistry and Molecular Biology最新文献

{"title":"Epigenetics’ responsibility in endometriosis: A comprehensive assessment","authors":"Oyedayo Phillips Akano ,&nbsp;Victor Oluwaloseyi Amos ,&nbsp;Israel Opeyemi Fawole ,&nbsp;Adewole Christianah Oyindamola ,&nbsp;Ayodeji Folorunsho Ajayi","doi":"10.1016/j.jsbmb.2025.106814","DOIUrl":"10.1016/j.jsbmb.2025.106814","url":null,"abstract":"<div><div>Endometriosis is a chronic inflammatory disease characterized by the presence of endometrial-like tissue outside the uterus, affecting women of reproductive age. Despite extensive research, its pathophysiology remains unclear, with genetic, hormonal, and environmental factors playing interconnected roles. Epigenetic processes, including non-coding RNAs, histone modifications, and DNA methylation, have been implicated in the genesis and progression of endometriosis. These modifications impact physiological functions such as inflammation, cell division, apoptosis, and hormone sensitivity. Recent findings on epigenetic alterations in endometriosis highlight their role in the abnormal behavior of ectopic endometrial-like cells. Aberrant DNA methylation patterns in genes related to immunological control and oestrogen metabolism contribute to the invasiveness and durability of lesions. Histone modifications, such as methylation and acetylation, regulate gene expression by altering chromatin structure. Non-coding RNAs, particularly microRNAs, influence tissue remodeling and inflammation. Given the reversible nature of epigenetic modifications, they present promising therapeutic targets for innovative treatment strategies. Epigenetic-based therapies aim to reverse pathological gene expression patterns, offering hope for more personalized and effective management of endometriosis. Further research is needed to fully utilize epigenetic processes in treating this debilitating disease.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106814"},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological gender difference of bile acid metabolism in susceptibility to cholelithiasis in patients with nonalcoholic fatty liver disease","authors":"Ya Wang ,&nbsp;Dazhang Deng ,&nbsp;Meiqing Mai ,&nbsp;Wanhan Song ,&nbsp;Qianhua Yuan ,&nbsp;Yutong Xie ,&nbsp;Bingquan Mo ,&nbsp;Honghui Guo","doi":"10.1016/j.jsbmb.2025.106812","DOIUrl":"10.1016/j.jsbmb.2025.106812","url":null,"abstract":"<div><div>The incidence of gallstone disease (GSD) is often higher in female patients with nonalcoholic fatty liver disease (NAFLD) compared to their male counterparts; however, the underlying mechanisms remain unclear. This cross-sectional study aimed to investigate gender differences in susceptibility to GSD with a focus on the specificity of bile acid (BA) metabolism, while also considering the reciprocal influence between gut bacteria and BAs. A total of 16 female and 36 male NAFLD patients, identified as having a high risk of GSD, were included in the study. Serum and fecal BA profiles were analyzed, along with a correlation analysis of gut microbiota composition. Gender-specific divergence in hydrophobic BAs distribution was observed, with females exhibiting significantly higher serum retention. The concentrations of serum glycolithocholic acid, isolithocholic acid, and 12-ketolithocholic acid, as well as fecal lithocholic acid, were significantly higher in females than in males. Conversely, the levels of serum hyodeoxycholic acid, fecal 7-ketolithocholic acid (7-keto-LCA), chenodeoxycholic acid, glycocholic acid, and ursodeoxycholic acid were significantly lower in females. Additionally, the total abundance of <em>Fusobacterium</em> and <em>Escherichia-Shigella</em> in the gut microbiota was elevated in females. A statistically significant inverse correlation was observed in females between reduced fecal 7-keto-LCA levels and an increased abundance of butyrate-producing bacterial communities. Overall, BAs and gut microbiota exhibit gender dimorphism in NAFLD patients, which may be linked to the increased risk of secondary GSD in women.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106812"},"PeriodicalIF":2.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of amh and its potential signaling pathway involved in the ovary development in the largemouth bass (Micropterus salmoides)","authors":"Huifen Liu ,&nbsp;Yueying Wang ,&nbsp;Qi Guo ,&nbsp;Xue Tian ,&nbsp;Xiao Ma ,&nbsp;Meng Zhang ,&nbsp;Xi Shi ,&nbsp;Wenge Ma ,&nbsp;JianXin Zhang ,&nbsp;Ruwei Xu ,&nbsp;Xuejun Li ,&nbsp;Xianghui Kong","doi":"10.1016/j.jsbmb.2025.106811","DOIUrl":"10.1016/j.jsbmb.2025.106811","url":null,"abstract":"<div><div>Anti-müllerian hormone (AMH), a member of the TGF-β family, plays a pivotal role in ovarian development by binding to its specific receptor, AMHR2. However, the function of <em>amh/amhr2</em> pathway in fish ovarian development remains poorly understood. To elucidate its regulatory mechanism in largemouth bass, we identified <em>amh</em> and <em>amhr2</em> genes from the largemouth bass genome and analyzed their expression patterns across different tissues and ovarian developmental stages. The results showed that <em>amh</em> was mainly expressed in the testis, ovary, brain and pituitary, while <em>amhr2</em> was highly expressed in the testis and ovary. In <em>vitro</em> treatment of ovarian follicles with recombinant Amh protein increased GVBD rates of cultured oocytes, and the expression levels of <em>amh</em> receptors (<em>amhr2</em>, <em>alk2</em>, <em>alk3</em> and <em>alk6</em>) and <em>smad</em> signaling pathway (<em>smad1</em>, <em>smad5</em>, <em>smad8</em> and <em>smad4</em>) were significantly upregulated. Additionally, the expression of key steroidogenesis (<em>stAR</em>, <em>17βhsd</em>, <em>cyp17a2</em>, <em>cyp19a1a</em>) and oocyte maturation-related genes (<em>cyclin B</em>, <em>cdk1</em>) were also increased. Conversely, inhibition of Amhr1 with Compound C attenuated Amh-induced upregulation of Amh receptors, SMADs, and steroidogenic genes. These findings provide novel insights into the molecular mechanisms by which the <em>amh/amhr2</em> signaling pathway regulates ovarian development in largemouth bass, highlighting its role in follicular maturation and steroidogenesis.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106811"},"PeriodicalIF":2.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D status in young adults with cystic fibrosis on highly effective CFTR modulator therapy","authors":"Crystal Cobb ,&nbsp;Jessica A. Alvarez ,&nbsp;William R. Hunt ,&nbsp;Tanicia Daley ,&nbsp;Shasha Bai ,&nbsp;Vin Tangpricha","doi":"10.1016/j.jsbmb.2025.106810","DOIUrl":"10.1016/j.jsbmb.2025.106810","url":null,"abstract":"<div><div>Vitamin D deficiency is a common pathology in people with cystic fibrosis (PwCF) due to the malabsorption of fat-soluble vitamins. Vitamin D plays an integral role in bone health and lung immunity; therefore, treating deficiencies is a clinical priority in PwCF. Highly effective modulator therapy (HEMT) improves the function of the cystic fibrosis transmembrane conductance regulator (CFTR) protein that is altered in PwCF, resulting in improved lung function and fat absorption. However, the impact of HEMT on restoring vitamin D status, a fat-soluble vitamin, has not been fully elucidated. We retrospectively examined serum 25-hydroxyvitamin D (25(OH)D) up to ten years prior in 89 young adults with CF classified based on current HEMT use (yes or no). We used two-way ANOVA to evaluate trends in both groups. Both HEMT users (n = 68) and HEMT non-users (n = 21) on average exhibited decreased serum 25(OH)D levels over ten years (-14.2 ng/mL (SI 35.4 nmol/L) and −14 ng/mL (SI 34.9 nmol/L) respectively), with no difference in change between the two groups (p = 0.44). This suggests that HEMT may not correct vitamin D status in PwCF. Further large scale prospective studies are needed to comprehensively investigate the relationship between vitamin D and HEMT.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106810"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-androgenic and anti-proliferative properties of methanolic leaf extract of Allamanda cathartica Linn. in adult mouse testis: Evidences from in vivo and in silico studies","authors":"Rakesh Raman ,&nbsp;Rimple Kaur ,&nbsp;Swanand Kulkarni ,&nbsp;Deepanshu Joshi ,&nbsp;Jyoti Parkash ,&nbsp;Suresh Thareja ,&nbsp;Debarshi Sarkar ,&nbsp;Shio Kumar Singh","doi":"10.1016/j.jsbmb.2025.106798","DOIUrl":"10.1016/j.jsbmb.2025.106798","url":null,"abstract":"<div><div>Dysregulation in androgen production causes a variety of clinical disorders like male pattern baldness, hirsutism, acne vulgaris, benign prostatic hyperplasia, prostate cancer and polycystic ovarian syndrome. Although the common synthetic drugs used to treat these diseases have many side effects, there is growing demand for alternative and herbal therapies. <em>Allamanda cathartica</em> Linn. (family, <em>Apocynaceae</em>) is traditionally used to treat variety of diseases because of its hepatoprotective, antidiabetic, anti-inflammatory, antitumor and antimicrobial properties. However, the effects of Allamanda leaves on testosterone production still remain elusive. The present study aims to elucidate the effect and possible mode(s) of action of the methanolic leaf extract of <em>A. cathartica</em> (MLEAC) on androgen biosynthesis and germ cell proliferation in adult mouse testis. Adult male C57BL/6J mice were orally administered MLEAC (150 and 300 mg/kg body weight/day) or distilled water (controls) for 42 days. MLEAC treatment caused non-uniform degenerative changes in the histoarchitecture of testis. The treatment also had negative impact on serum testosterone level with downregulation of the expressions of major steroidogenic proteins (StAR, CYP11A1, 3β-and 17β-HSD3). Flow cytometry and immunohistochemical analyses showed impaired gem cell proliferation in MLEAC-treated mice testes. The GC-MS method identified 20 phytocompounds in MLEAC. The <em>in silico</em> study further revealed that GC-MS-derived phytochemicals have the potential to bind with major steroidogenic proteins in testis. MLEAC treatment thus causes suppression of germ cell proliferation via downregulation of testosterone production. Keeping in view the traditional use of Allamanda, the present findings may prove helpful in the search of a plant-based anti-androgenic compound.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106798"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D deficiency: A risk factor for antibody decline in COVID-19?","authors":"Luciana Paola Bohl ,&nbsp;María Laura Breser ,&nbsp;Gabriela Edith Aguirre ,&nbsp;María Ingrid Capello ,&nbsp;Gerardo Ivan Menichetti ,&nbsp;Georgina Tiraboschi ,&nbsp;Lucía Rodríguez-Berdini ,&nbsp;Paula Isaac ,&nbsp;María de los Ángeles Fernández ,&nbsp;Carina Porporatto","doi":"10.1016/j.jsbmb.2025.106809","DOIUrl":"10.1016/j.jsbmb.2025.106809","url":null,"abstract":"<div><div>Vaccination was critical in controlling the transmission of SARS-CoV-2 during the COVID-19 pandemic. Nevertheless, immune responses are also influenced by nutritional and endocrine factors, particularly vitamin D, whose deficiency has been linked to an increased risk and severity of respiratory infections. This study aimed to assess the impact of 25-hydroxyvitamin D (25(OH)D) levels on the humoral immune response to SARS-CoV-2, as measured by specific immunoglobulin G (IgG) antibody levels. Associations with other factors were also explored, including symptoms, pre-existing conditions, COVID-19 history, vaccination status, and medication use during the course of infection. A longitudinal study was conducted with 131 adult patients from Villa del Rosario (Argentina) who tested positive for SARS-CoV-2 between August and December 2021. Data on weight, age, height, gender, symptoms, pre-existing conditions, vitamin D sources, and COVID-19 history were collected upon diagnosis, as well as 30 and 180 days post-diagnosis. Eighty-five percent of the patients experienced mild COVID-19; 65 % had low vitamin D levels, and individuals with pre-existing conditions had significantly lower 25(OH)D levels. The highest antibody levels were observed one month after diagnosis. Vaccinated patients exhibited higher specific antibody levels than unvaccinated ones. Those who used palliative medication for symptom control had lower levels of specific antibodies. Finally, antibody levels decreased significantly between day 30 and day 180 post-diagnosis in patients with vitamin D deficiency. These findings emphasize the importance of maintaining adequate vitamin D levels for sustained immune responses, and highlight the role of vaccination in enhancing immune protection against COVID-19.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106809"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frontiers in steroid research: Steroid congress 2023","authors":"Trevor M. Penning,&nbsp;Jerzy Adamski","doi":"10.1016/j.jsbmb.2025.106808","DOIUrl":"10.1016/j.jsbmb.2025.106808","url":null,"abstract":"","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106808"},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untangling hidden players of PCOS: From transcriptomics to key biomarkers using WGCNA, LASSO and ROC analysis","authors":"Harshini Senthilkumar,&nbsp;Mohanapriya Arumugam","doi":"10.1016/j.jsbmb.2025.106795","DOIUrl":"10.1016/j.jsbmb.2025.106795","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder characterized by hormonal imbalance, inflammation and insulin resistance. This study utilized a comprehensive RNA-Seq workflow to uncover the molecular mechanisms underlying PCOS. Data were retrieved from the NCBI- Sequence Read Archive [SRA] repository, followed by quality assessment using FastQC and Trimmomatic trimming. Reads were aligned to the GRCh38 genome using HISAT2 and differential expression analysis was performed by DESeq2 in R, identifying 395 significant DEGs (268 upregulated, 127 downregulated). Gene ontology (GO) analysis revealed dysregulated biological processes including activation of protein kinase activity, telomerase capping, protein serine/ threonine kinase activity and MAP kinase kinase activity, while KEGG pathway analysis highlighted key signaling pathways such as MAPK signaling, Ras signaling, and GnRH signaling. Weighted gene co-expression network analysis (WGCNA) identified modules associated with PCOS traits and Protein-Protein Interaction (PPI) network analysis identified 20 hub genes. Through Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis, five key predictor genes – <em>PRKACA</em>, <em>SREBF2</em>, <em>MAPT</em>, <em>EHMT1</em>, and <em>JUP</em> were identified, highlighting their critical roles in the pathogenesis of PCOS. The prognostic value of these genes was further validated by receiver operating characteristics (ROC) curve analysis, which showed a high area under the curve (AUC) of 0.89. This strong discriminatory power between PCOS and healthy groups highlights the potential of these biomarkers as reliable indicators for diagnosing and understanding the disease. The results highlight the therapeutic and diagnostic potential of these biomarkers and pave the way for precision medicine approaches in PCOS treatment.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106795"},"PeriodicalIF":2.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of reference ranges of four circulating sex steroids from dried blood spots in women aged 18-40 years","authors":"Yuanyuan Wang ,&nbsp;Susan R. Davis ,&nbsp;Josh Gialouris ,&nbsp;Reena Desai ,&nbsp;David J. Handelsman","doi":"10.1016/j.jsbmb.2025.106796","DOIUrl":"10.1016/j.jsbmb.2025.106796","url":null,"abstract":"<div><div>Capillary dried blood spot collection offers an excellent less invasive alternative to serum or plasma collection by venesection. This study aimed to generate reference ranges for four sex steroids in capillary whole blood samples collected from healthy premenopausal women as dried blood spots as a screening method to detect disorders of sex development. Using this method we evaluated variations according to age, body mass index, menstrual cycle, and hormonal contraceptive use. This cross-sectional, community-based study included 120 healthy premenopausal women aged 18-40 years, not pregnant or breastfeeding. Whole blood testosterone (T), androstenedione (A4), 17α hydroxyprogesterone (17OHP), and progesterone (P4) were measured by liquid chromatography-tandem mass spectrometry (LCMS) from dried filter paper blood spots. The median (95 % confidence limits) for T were 0.75 (0.28, 2.20) nmol/L, A4 2.69 (1.02, 6.03) nmol/L, 17OHP 1.62 (0.32, 8.52) nmol/L and P4 0.65 (0.18, 41.0) nmol/L. Age by 5-year interval was associated with a progressive reduction in A4 but not for other steroids. Menstrual variation was evident for 17OHP and P4 with increases in luteal phase with a small mid-cycle peak for A4 but no other consistent changes. All sex steroid concentrations were significantly lower in participants taking a systemic hormonal contraceptive (combined oral contraceptive or etonogestrel implant). None of the sex steroid concentrations varied significantly with body mass index. These data provide reference ranges for whole blood T, A4, 17OHP and P4 measured by LCMS from dried blood spots from healthy young adult premenopausal women. Small but significant variations were evident according to age, hormonal contraceptive use and menstrual cycle phase.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106796"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced and rearranged metabolite structures after metandienone administration: New promising metabolites for potential long-term detection","authors":"Jakob Steff ,&nbsp;Francesco Molaioni ,&nbsp;Nils Schlörer ,&nbsp;Xavier de la Torre ,&nbsp;Matthias Bureik ,&nbsp;Francesco Botrè ,&nbsp;Maria Kristina Parr","doi":"10.1016/j.jsbmb.2025.106801","DOIUrl":"10.1016/j.jsbmb.2025.106801","url":null,"abstract":"<div><div>Metandienone (MD) is a representative of the group of anabolic androgenic steroids and is commonly used in professional and amateur sports despite being a banned substance by the World Anti-Doping Agency (WADA). Metabolites of MD show high structural similarity to related anabolic androgenic steroids (AAS) such as dehydrochloromethyltestosterone (DHCMT). This led to the hypothesis that metabolites of MD with structures similar to long-term metabolites of DHCMT may be detectable. Therefore, a human administration study of MD was carried out and analyzed with the focus on metabolite structures with partly or fully reduced A-rings and eventually with rearranged D-rings with 17ξ-hydroxymethyl-17ξ-methyl substructures. Synthesized diastereomeric reference material allowed the establishment of a confident identification and characterization of excreted targeted compounds by gas chromatography-mass spectrometry. In this way, the excretion of <em>inter alia</em> 17α-methyl-5β-androstane-3α,17β-diol (<strong>T3</strong>), 17β-methyl-5β-androstane-3α,17α-diol (<strong>T7</strong>), 17α-hydroxymethyl-17β-methyl-18-nor-5β-androst-13-en-3α-ol (<strong>N3</strong>), 17α-hydroxymethyl-17β-methyl-18-nor-5β-androsta-1,13-dien-3α-ol (<strong>E3</strong>) and 17,17-dimethyl-18-nor-5β-androst-13-en-3α-ol (3α5βnorTHMT) was confirmed. Excretion curves of previously known and newly discovered metabolites enabled the assessment of the relationship between the chemical structure and the time of excretion. In addition to further assembling the picture of human metabolism of AAS with newly discovered metabolites, the detection of <strong>E3</strong> allows the presumption to be a promising future candidate for a new long-term marker in anti-doping analyses with indications for an increased detection window.</div></div>","PeriodicalId":51106,"journal":{"name":"Journal of Steroid Biochemistry and Molecular Biology","volume":"253 ","pages":"Article 106801"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}