Fish Pathology最新文献_第8页

Bucephalid Metacercarial Infection in Wild Larval and Juvenile Ayu Plecoglossus altivelis 野生阿育银舌鱼幼鱼双头蚴感染的研究

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2020-01-15 DOI: 10.3147/jsfp.54.93

S. Shirakashi, Tsukasa Waki, K. Ogawa

{"title":"Bucephalid Metacercarial Infection in Wild Larval and Juvenile Ayu Plecoglossus altivelis","authors":"S. Shirakashi, Tsukasa Waki, K. Ogawa","doi":"10.3147/jsfp.54.93","DOIUrl":"https://doi.org/10.3147/jsfp.54.93","url":null,"abstract":"Ayu Plecoglossus altivelis is a native amphidromous fish in Japan and an important target for farming and recreational fishing. We have found high infection of hitherto unknown digenean metacercariae in wild ayu larvae and juveniles captured from the sea and a river in Wakayama Prefecture. The infection prevalence was 95% and 100% in larvae and juvenile ayu, respectively with variable intensities up to 566 metacercariae per fish. The metacercariae were located primarily around fins and inside caudal fins, but appeared to cause no serious pathological features to the fish. The phylogenetic analysis based on the internal transcribed spacer 2 and 28S rDNA sequences revealed its identity as a member of Bucephalinae (Bucephalidae) with no identical genetic information on the database. Morphological characteristics of the larval trematode resembled those of the genus Prosorhynchoides. Genetically identical metacercariae were also found from Scomber japonicus and Engraulis japonicus captured at the same location as P. altivelis with variable infection levels, which suggested that it was a marine species. Experimental infection challenge of the metacercariae to several marine fish via oral intubation failed to obtain adult specimen and thus the precise identification of the parasite remains unknown.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":"27 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75474393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Identification of the Epitope Recognized by the Anti-Red Sea Bream Iridovirus (RSIV) Monoclonal Antibody M10 Using a Phage Display RSIV Peptide Library 利用噬菌体展示RSIV肽库鉴定抗红鲷虹膜病毒(RSIV)单克隆抗体M10识别的表位

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2020-01-15 DOI: 10.3147/jsfp.54.83

T. Takano, T. Matsuyama, Y. Kawato, T. Sakai, J. Kurita, Yuta Matsuura, Sachiko Terashima, K. Nakajima, C. Nakayasu

{"title":"Identification of the Epitope Recognized by the Anti-Red Sea Bream Iridovirus (RSIV) Monoclonal Antibody M10 Using a Phage Display RSIV Peptide Library","authors":"T. Takano, T. Matsuyama, Y. Kawato, T. Sakai, J. Kurita, Yuta Matsuura, Sachiko Terashima, K. Nakajima, C. Nakayasu","doi":"10.3147/jsfp.54.83","DOIUrl":"https://doi.org/10.3147/jsfp.54.83","url":null,"abstract":"Red sea bream iridoviral disease (RSIVD) spreads readily throughout the marine environment because of its wide variety of host species. Use of a rapid diagnostic method, such as the immunofluorescence antibody test, helps to control the disease, so the anti-RSIV monoclonal antibody M10 (mAb M10) has been developed in Japan. In the present study, we carried out epitope mapping using the phage display method to identify the antigen of mAb M10. A phage display RSIV peptide library was constructed to cover the entire genome of RSIV, then phage clones recognized by the antibody were selected by biopanning. The selected clones harbored partial fragments of the laminin-type epidermal growth factor-like domain (LEGFD) gene. N-terminal and C-terminal deletion peptides were then prepared from the amino acid sequence deduced from the smallest fragment to precisely determine the epitope. Finally, seven amino acids, EYDCPEY, located in the extracellular domain of the LEGFD protein were determined to be the epitope. Identical residues of the epitope were also identified from the LEGFD protein in other megalocytiviruses including the infectious spleen and kidney necrosis virus and turbot reddish body iridovirus. mAb M10 is considered to be widely available for the diagnostics of megalocytivirus infections.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":"14 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83515188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of Hydrogen Peroxide-Melanin Bleaching and Fluorescent Nuclear Staining for Whole-Body Clearing and Imaging in Fish 双氧水-黑色素漂白及荧光核染色在鱼类全身清除成像中的应用

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2020-01-15 DOI: 10.3147/jsfp.54.101

K. Futami, Oto Furukawa, M. Maita, T. Katagiri

引用次数: 3

Development of a Real-time PCR to Detect Genotypes A and B in Flavobacterium psychrophilum 嗜冷黄杆菌a、B基因型实时PCR检测方法的建立

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-09-15 DOI: 10.3147/jsfp.54.58

Ryo Inoue, T. Takase

引用次数: 0

Skin Injuries Contribute to Nocardiosis in Japanese Amberjack Seriola quinqueradiata 皮肤损伤与日本琥珀诺卡菌病有关

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-09-15 DOI: 10.3147/jsfp.54.64

Y. Miyoshi, Y. Fukuda, K. Ogawa

引用次数: 3

Evaluation of Pathogenicity of Edwardsiella ictaluri to Riverine Fish Species Using an Immersion Infection Method 用浸渍法评价伊氏爱德华氏菌对河流鱼类的致病性

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-09-15 DOI: 10.3147/jsfp.54.61

Hisato Takeuchi, D. Nakano, Mayu Iwadare, Ryuji Tomono, T. Imai, Kazutomo Hori, Hiroaki Izumi, A. Namba, Sonoko Shimizu, T. Nakai, N. Mano

引用次数: 4

Epidemiological Study on Newly Emerging Lactococcus garvieae Serotype II Isolated from Marine Fish Species in Japan 日本海鱼中新分离的鸡乳球菌血清型流行病学研究

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-09-15 DOI: 10.3147/jsfp.54.51

Yin-Ze Shi, I. Nishiki, S. Yanagi, Terutoyo Yoshida

引用次数: 7

A Survey of Water Temperature for Production of Juvenile Ayu Plecoglossus altivelis 阿育鱼幼鱼生产水温的调查

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-06-15 DOI: 10.3147/jsfp.54.40

引用次数: 1

Mortality of Spats of Manila Clam Ruditapes philippinarum Experimentally Challenged with the Protozoan Parasite Perkinsus olseni 原生寄生虫奥氏珀金丝虫侵染菲律宾马尼拉蛤幼体的实验研究

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-06-15 DOI: 10.3147/JSFP.54.34

Tsukasa Waki, T. Yoshinaga

引用次数: 1

Phylogenetic Analysis with Complete Mitochondrial Genome Sequences of Benedenia seriolae Specimens Derived from Japanese Seriola spp. 日本小孢子虫标本线粒体全基因组序列的系统发育分析。

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2019-06-15 DOI: 10.3147/JSFP.54.27

S. Kawato, Keigo Kobayashi, S. Shirakashi, S. Yanagi, Y. Fukuda, H. Yamashita, R. Nozaki, I. Hirono, H. Kondo

{"title":"Phylogenetic Analysis with Complete Mitochondrial Genome Sequences of Benedenia seriolae Specimens Derived from Japanese Seriola spp.","authors":"S. Kawato, Keigo Kobayashi, S. Shirakashi, S. Yanagi, Y. Fukuda, H. Yamashita, R. Nozaki, I. Hirono, H. Kondo","doi":"10.3147/JSFP.54.27","DOIUrl":"https://doi.org/10.3147/JSFP.54.27","url":null,"abstract":"― Infection with the skin ﬂuke Benedenia seriolae is a serious threat of yellowtail aquaculture. Development of genomic resources and molecular markers for B. seriolae is an essential infrastructure to develop control strategies against B. seriolae infections. As a seminal study to establish the genomic resources of the Japanese B. seriolae populations, we sequenced the complete mitochondrial genome (mitogenome) sequences of 12 B. seriolae specimens collected from four prefectures in Japan. The Japanese B. seriolae mitogenomes were mutually 99% identical, while the identity was 85% when compared to that of an Australian specimen. Furthermore, the gene arrangement in the Japanese B. seriolae mitogenome was slightly different from that in the Australian specimen. The substantial mitogenomic divergence between the Australian and Japanese specimens indicates that B. seriolae is composed of molecularly divergent populations. Variable sites were dispersed in the mitogenome of the Japanese B. seriolae . Phylogenetic relationships among the Japanese B. seriolae specimens did not reﬂect the host species or geographic distributions within Japan.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":"26 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80173504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4