针对gyrB区嗜冷黄杆菌的新型PCR-RFLP基因分型

IF 0.6 4区农林科学 Q4 FISHERIES

Fish Pathology Pub Date : 2019-06-15 DOI:10.3147/JSFP.54.37

S. Izumi, H. Arai, Kyuma Suzuki, F. Aranishi

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引用次数: 2

摘要

-利用嗜冷黄杆菌DNA旋切酶基因(gyrB) B亚基和限制性内切酶Bsp 119I特异性PCR引物，建立了一种新的PCR- rflp分型方法。利用该PCR-RFLP方法，将300株嗜冷致病菌分离株分为197株和103株两种基因型，并与分离株所属的寄主鱼类进行了相关分析。结合之前的三个PCR-RFLPs，将这些分离物进一步细分为12个基因型。提示该PCR-RFLP分型系统可作为细菌性冷水病流行病学研究的有效工具。

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A Novel PCR-RFLP Genotyping of Flavobacterium psychrophilum Targeting the gyrB Region

― A novel PCR-RFLP typing method was developed using PCR primers speciﬁc for the B subunit of DNA gyrase gene ( gyrB ) of Flavobacterium psychrophilum and restriction endonuclease Bsp 119I. With this PCR-RFLP, 300 isolates of F. psychrophilum were classiﬁed into two genotypes (197 and 103 isolates), and the respective genotypes were correlated with the host ﬁsh species from which the isolates were derived. These isolates were further subdivided into 12 genotypes by combining with three previous PCR-RFLPs. It is suggested that this PCR-RFLP typing system can be a useful tool in epizootiological studies on bacterial cold-water disease.

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