Fish Pathology最新文献_第10页

Mass Mortality Caused by Pseudomonas anguilliseptica in the Pond-cultured Ayu Plecoglossus altivelis 池塘养殖Ayu pleclossus altivelis中嗜蛇假单胞菌引起的大量死亡

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-09-15 DOI: 10.3147/JSFP.53.101

Y. Shimahara, Y. Kawato, I. Kiryu, T. Nishioka, T. Kamaishi, K. Yuasa, S. Miwa, Mei Hosaka, Ryoko Matsumoto, T. Nakai, N. Oseko

引用次数: 1

An Oral Vaccination Method with the Aid of Capsaicin against Viral Nervous Necrosis (VNN) 辣椒素口服预防病毒性神经坏死(VNN)的方法

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-09-15 DOI: 10.3147/JSFP.53.110

A. Gaafar, H. Yamashita, I. Istiqomah, Y. Kawato, Kanae Ninomiya, A. Younes, T. Nakai

引用次数: 7

Experimental Infection of Ostreid Herpesvirus 1 (OsHV-1) JPType1, a Japanese Variant, in Pacific Oyster Crassostrea gigas Larvae and Spats 日本变种Ostreid Herpesvirus 1 (OsHV-1) JPType1在太平洋牡蛎长牡蛎幼虫和幼鱼中的实验感染

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-06-15 DOI: 10.3147/jsfp.53.71

T. Nagai, Misato Nakamori

{"title":"Experimental Infection of Ostreid Herpesvirus 1 (OsHV-1) JPType1, a Japanese Variant, in Pacific Oyster Crassostrea gigas Larvae and Spats","authors":"T. Nagai, Misato Nakamori","doi":"10.3147/jsfp.53.71","DOIUrl":"https://doi.org/10.3147/jsfp.53.71","url":null,"abstract":"In many locations worldwide, ostreid herpesvirus 1 (OsHV-1) infections are associated with mortalities in Pacific oyster Crassostrea gigas primarily during the summer months. In the present study, experimental infections were performed to investigate the pathogenicity of OsHV・1JPType1 (a Japanese variant) in hatchery-reared Pacific oyster larvae and spats. The JPType1 virus was prepared from dead oyster spats obtained from a sea cage and was once passed in oyster larvae as the inoculum source of the infection experiments. Compared to pediveliger larvae, D-shaped larvae were more susceptible to infections when the larvae were exposed to serially diluted virus solutions at the concentrations of 3.2 x 106, 3.2 x 105 and 3.2×104 virus DNA copies/ml. Real-time PCR analysis revealed 1.6× 108 virus DNA copies/ng total DNA in a pooled sample of D-shaped larvae. No mortality was observed after 5 days when nine spat groups of differently sizes (mean shell height from 1.8 mm to 6.1 mm) were immersed in seawater containing 2.5×106 virus DNA copies/ml; however, 6.3×105 virus DNA copies/mg whole weight were detected in a group of spats. Virus concentrations were higher in smaller spat groups than in larger spat groups. The pathogenicity of OsHV司 1JPType1 in larvae and spats was confirmed; however, i t declined with the growth of the oyster.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73028029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Aeromonas veronii biovar sobria Associated with Mortalities of Riverine Ayu Plecoglossus altivelis in the Tama River 与多摩河阿玉斑舌鱼死亡率相关的维氏气单胞菌生物变种sobria

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-06-15 DOI: 10.3147/JSFP.53.86

Hisato Takeuchi, A. Namba, Kazutomo Hori, S. Kashiwada, N. Mano

引用次数: 2

Francisella halioticida, Identified as the Most Probable Cause of Adductor Muscle Lesions in Yesso scallops Patinopecten yessoensis Cultured in Southern Hokkaido, Japan 日本北海道南部养殖的扇贝(Patinopecten yessoensis)内收肌病变最可能的原因是盐纹Francisella haloticida

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-06-15 DOI: 10.3147/JSFP.53.78

Miku Kawahara, M. Kanamori, G. Meyer, T. Yoshinaga, N. Itoh

{"title":"Francisella halioticida, Identified as the Most Probable Cause of Adductor Muscle Lesions in Yesso scallops Patinopecten yessoensis Cultured in Southern Hokkaido, Japan","authors":"Miku Kawahara, M. Kanamori, G. Meyer, T. Yoshinaga, N. Itoh","doi":"10.3147/JSFP.53.78","DOIUrl":"https://doi.org/10.3147/JSFP.53.78","url":null,"abstract":"The occurrence of orange/pinkish colored lesions in the adductor muscle of Yesso scallops Patinopecten yessoensis has been known for many years in Japan; however, determination of the causative agent has not been adequately investigated. Histological examination of affected scallops in southern Hokkaido typically revealed intense host responses: hemocyte infiltration, an abundance of necrotic hemocytes, lysis of muscle fibers and in some instances melanin deposits when the lesions occurred adjacent to the shell. Microbiota analysis showed that Francisella halioticida was dominant in the lesions, and in situ hybridization using F. halioticida specific probes also confirmed the presence of this bacterium within the lesions. A F. halioticida specific PCR assay detected this bacterium in the majority of scallop lesions tested. Subsequently, three bacterial isolates were obtained from scallop lesions on modified Eugon agar supplemented with antibiotics, and these bacterial isolates were found to be F. halioticida by 16S rRNA and rpoB gene sequences. These results suggest that infection with F. halioticida is the most likely cause of the adductor muscle lesions observed in Yesso scallops. Field surveys conducted in 2017 of scallops cultured in southern Hokkaido showed that the presence of adductor muscle lesions putatively caused by F. halioticida was significantly related to mortalities and poor growth of scallops.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82441792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

An Epidemiological Study of Akoya Oyster Disease Using Polymerase Chain Reaction Targeting Spirochaetes Genes 以螺旋体基因为靶点的聚合酶链反应研究阿古雅牡蛎病的流行病学

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-06-15 DOI: 10.3147/JSFP.53.63

T. Matsuyama, Yuta Matsuura, M. Inada, T. Takano, C. Nakayasu, T. Sakai, Sachiko Terashima, M. Yasuike, A. Fujiwara, Yoji Nakamura, Y. Tsuchihashi, K. Odawara, S. Iwanaga, T. Masaoka

{"title":"An Epidemiological Study of Akoya Oyster Disease Using Polymerase Chain Reaction Targeting Spirochaetes Genes","authors":"T. Matsuyama, Yuta Matsuura, M. Inada, T. Takano, C. Nakayasu, T. Sakai, Sachiko Terashima, M. Yasuike, A. Fujiwara, Yoji Nakamura, Y. Tsuchihashi, K. Odawara, S. Iwanaga, T. Masaoka","doi":"10.3147/JSFP.53.63","DOIUrl":"https://doi.org/10.3147/JSFP.53.63","url":null,"abstract":"In a previous study, a member of Spirochaeta was presumed to cause Akoya oyster disease (AOD), and Candidatus Maribrachyspira akoyae was proposed as the putative causative agent. With the aim of performing an epidemiological study of AOD, polymerase chain reaction (PCR) assays were developed using the primers designed from shotgun metagenomic sequences identified as having high homology to Spirochaetes genes by a BLAST search. PCR assays with six primer sets produced specific target amplicons (n = 63–67) in pearl oysters collected from five AOD-endemic areas (n = 88), but did not in pearl oysters (n = 36) collected from three AOD-free areas. All individuals (n = 17) with the color index a-value above 3.0, which is an index of AOD development, were positive for all 6 primer sets in PCR. There was a positive correlation between the a-value and the results of PCR. These results show that the previous identification of Spirochaeta as an etiological agent of AOD is reasonable. Sequencing of amplification products confirmed only one nucleotide substitution in the PCR products, suggesting that the pathogenic causative agent of AOD has limited genetic diversity.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75451655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Observation of the Micropyle of Unfertilized and Water-Hardening Rainbow Trout Eggs by Scanning Electron Microscopy 未受精卵和水硬化虹鳟鱼卵微孔的扫描电镜观察

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-06-15 DOI: 10.3147/jsfp.53.90

引用次数: 0

Percentage dynamics of CD4-1+ and CD8α+ T cells in different tissues at various developmental stages in ginbuna crucian carp Carassius auratus langsdorfii. 金银鲫不同发育阶段不同组织中CD4-1+和CD8α+ T细胞的百分比动态。

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-01-01 DOI: 10.3147/JSFP.53.10

M. Khallaf, Shino Ogawa, H. Toda, Yuta Matsuura, Ryuichiro Miyazawa, T. Nakanishi

{"title":"Percentage dynamics of CD4-1+ and CD8α+ T cells in different tissues at various developmental stages in ginbuna crucian carp Carassius auratus langsdorfii.","authors":"M. Khallaf, Shino Ogawa, H. Toda, Yuta Matsuura, Ryuichiro Miyazawa, T. Nakanishi","doi":"10.3147/JSFP.53.10","DOIUrl":"https://doi.org/10.3147/JSFP.53.10","url":null,"abstract":"Determination of numbers of T cell subsets in tissues is important to assess the functional maturity of the immune system. The distribution of T cells in lymphoid tissues during ontogenic development has been examined in several fish species using pan-T cell markers. However, information on the age-related changes in the number of T cell subsets in tissues is limited due to the lack of antibodies against CD4 and CD8 in fish. In the present study we examined the percentage dynamics of CD4-1 and CD8α cells in various tissues accompanied with age of ginbuna crucian carp using flow cytometry. We found rapid increases in both CD4-1 and CD8α T cells up until 6 months post-hatch (mph). CD4 and CD8 double-positive cells (DP cells) are present only in the thymus and DP cells increased, while double negative cells decreased, in fish up to 3–6 mph. The percentages of CD4-1 T cells were always higher than those of CD8α T cells in the various tissues examined, except for the thymus and intestine. In contrast, significantly higher percentages of CD8α T cells than CD4-1 T cells were found in the intestine during the period between 6 mph and 2 years post-hatch.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73200280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Comparative Proteomic Analysis Between Virulent and Less Virulent Strains of Edwardsiella ictaluri Isolated from Ayu Plecoglossus altivelis Ayu pleclossus altivelis Edwardsiella ictaluri强毒株与弱毒株的比较蛋白质组学分析

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-01-01 DOI: 10.3147/JSFP.53.29

T. Sakai, T. Kuwada, Y. Muto, T. Takano, K. Yuasa, N. Oseko

{"title":"Comparative Proteomic Analysis Between Virulent and Less Virulent Strains of Edwardsiella ictaluri Isolated from Ayu Plecoglossus altivelis","authors":"T. Sakai, T. Kuwada, Y. Muto, T. Takano, K. Yuasa, N. Oseko","doi":"10.3147/JSFP.53.29","DOIUrl":"https://doi.org/10.3147/JSFP.53.29","url":null,"abstract":"Outbreaks of disease caused by the infection of Edwardsiella ictaluri in ayu Plecoglossus altivelis have been reported in rivers in Japan since 2007. In this study, we performed comparative proteomic analysis between the virulent strain FPC1091 and the less virulent strain FPC1214 to identify virulence factors of E. ictaluri to ayu. In the experimental infection, cumulative mortalities of the ayu intraperitoneally injected with FPC1091 (1.2 × 10 CFU/fish) and FPC1214 (2.2 × 10 CFU/fish) were 100% and 26.7%, respectively. The genetic fingerprint profile of FPC1214 was identical to that of FPC1091 in the amplified-fragment length polymorphism (AFLP) analysis. The analysis of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry between FPC1091 and FPC 1214 revealed 23 proteins that were either specific to or produced in greater amounts in the virulent strain FPC1091. These proteins included components of the type III and type VI secretion systems, and the proteins that seem to be involved in the resistance against oxidative stress by host phagocytic cells, suggesting that these proteins are the virulence factors of E. ictaluri to ayu.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87443264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extracellular Proteinases of Miamiensis avidus Causing Scuticociliatosis are Potential Virulence Factors 引起褐条纤毛虫病的细胞外蛋白酶是潜在的毒力因子

IF 0.6 4区农林科学

Fish Pathology Pub Date : 2018-01-01 DOI: 10.3147/JSFP.53.1

Y. Narasaki, Y. Obayashi, S. Ito, Shoko Murakami, Jun-Young Song, K. Nakayama, S. Kitamura

{"title":"Extracellular Proteinases of Miamiensis avidus Causing Scuticociliatosis are Potential Virulence Factors","authors":"Y. Narasaki, Y. Obayashi, S. Ito, Shoko Murakami, Jun-Young Song, K. Nakayama, S. Kitamura","doi":"10.3147/JSFP.53.1","DOIUrl":"https://doi.org/10.3147/JSFP.53.1","url":null,"abstract":"Miamiensis avidus is the causative agent of scuticociliatosis in various marine fish species. The virulence factors of the parasite have not been identified, so far. In this study, we examined M. avidus extracellular proteinases (ECPs) as potential virulence factors, using culture supernatants as an ECPs source. We investigated the substrate specificity of ECPs using artificial peptides, and the cytotoxicity of the ECPs was examined using CHSE-214 cells. To elucidate the role of ECPs in ciliate growth, M. avidus was cultured on CHSE-214 cells in the presence of proteinase inhibitors. We detected proteinase activities from the supernatant of M. avidus. Viable CHSE-214 cells decreased significantly in number, when incubated in a medium supplemented with the culture supernatant of M. avidus. The growth of ciliates on CHSE-214 cells was delayed in the presence of PMSF (serine proteinase inhibitor) and E-64 (cysteine proteinase inhibitor). These results suggested that the culture supernatant contained ECPs showing cytotoxicity, and the proteinases facilitated nutrient uptake by the ciliates. Thus, ECPs may be responsible for virulence factors of M. avidus.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84142543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4