DNA Research最新文献_第9页

High-quality genome assembly of an important biodiesel plant, Euphorbia lathyris L. 重要生物柴油植物大戟的高质量基因组组装。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-10-11 DOI: 10.1093/dnares/dsab022

Mingcheng Wang, Zhijia Gu, Zhixi Fu, Dechun Jiang

{"title":"High-quality genome assembly of an important biodiesel plant, Euphorbia lathyris L.","authors":"Mingcheng Wang, Zhijia Gu, Zhixi Fu, Dechun Jiang","doi":"10.1093/dnares/dsab022","DOIUrl":"https://doi.org/10.1093/dnares/dsab022","url":null,"abstract":"Caper spurge, Euphorbia lathyris L., is an important energy crop and medicinal crop. Here, we generated a high-quality, chromosome-level genome assembly of caper spurge using Oxford Nanopore sequencing, Illumina sequencing, and Hi-C technology. The final genome assembly was ∼988.9 Mb in size, 99.8% of which could be grouped into 10 pseudochromosomes, with contig and scaffold N50 values of 32.6 and 95.7 Mb, respectively. A total of 651.4 Mb repetitive sequences and 36,342 protein-coding genes were predicted in the genome assembly. Comparative genomic analysis showed that caper spurge and castor bean clustered together. We found that no independent whole-genome duplication event had occurred in caper spurge after its split from the castor bean, and recent substantial amplification of long terminal repeat retrotransposons has contributed significantly to its genome expansion. Furthermore, based on gene homology searching, we identified a number of candidate genes involved in the biosynthesis of fatty acids and triacylglycerols. The reference genome presented here will be highly useful for the further study of the genetics, genomics, and breeding of this high-value crop, as well as for evolutionary studies of spurge family and angiosperms.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1d/7d/dsab022.PMC8545615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39530104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The time is ripe to investigate human centromeres by long-read sequencing†. 通过长读测序研究人类着丝粒的时机已经成熟。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-10-11 DOI: 10.1093/dnares/dsab021

Yuta Suzuki, Shinichi Morishita

引用次数: 3

Genome-wide identification and characterization of Fur-binding sites in the cyanobacteria Synechocystis sp. PCC 6803 and PCC 6714. 聚藻细菌PCC 6803和PCC 6714中皮毛结合位点的全基因组鉴定和表征

IF 4.1 2区生物学

DNA Research Pub Date : 2021-10-11 DOI: 10.1093/dnares/dsab023

Matthias Riediger, Miguel A Hernández-Prieto, Kuo Song, Wolfgang R Hess, Matthias E Futschik

{"title":"Genome-wide identification and characterization of Fur-binding sites in the cyanobacteria Synechocystis sp. PCC 6803 and PCC 6714.","authors":"Matthias Riediger, Miguel A Hernández-Prieto, Kuo Song, Wolfgang R Hess, Matthias E Futschik","doi":"10.1093/dnares/dsab023","DOIUrl":"https://doi.org/10.1093/dnares/dsab023","url":null,"abstract":"The Ferric uptake regulator (Fur) is crucial to both pathogenic and non-pathogenic bacteria for the maintenance of iron homeostasis as well as the defence against reactive oxygen species. Based on datasets from the genome-wide mapping of transcriptional start sites and transcriptome data, we identified a high confidence regulon controlled by Fur for the model cyanobacterium Synechocystis sp. PCC 6803 and its close relative, strain 6714, based on the conserved strong iron starvation response and Fur-binding site occurrence. This regulon comprises 33 protein-coding genes and the sRNA IsaR1 that are under the control of 16 or 14 individual promoters in strains 6803 and 6714, respectively. The associated gene functions are mostly restricted to transporters and enzymes involved in the uptake and storage of iron ions, with few exceptions or unknown functional relevance. Within the isiABC operon, we identified a previously neglected gene encoding a small cysteine-rich protein, which we suggest calling, IsiE. The regulation of iron uptake, storage, and utilization ultimately results from the interplay between the Fur regulon, several other transcription factors, the FtsH3 protease, and the sRNA IsaR1.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/61/dsab023.PMC8634477.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39537705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Exploring the evolutionary process of alkannin/shikonin O-acyltransferases by a reliable Lithospermum erythrorhizon genome. 利用一个可靠的红紫草基因组探索单宁/紫草素o -酰基转移酶的进化过程。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-09-13 DOI: 10.1093/dnares/dsab015

Chengyi Tang

{"title":"Exploring the evolutionary process of alkannin/shikonin O-acyltransferases by a reliable Lithospermum erythrorhizon genome.","authors":"Chengyi Tang","doi":"10.1093/dnares/dsab015","DOIUrl":"https://doi.org/10.1093/dnares/dsab015","url":null,"abstract":"Increasing genome data are coming out. Genome size estimation plays an essential role in guiding genome assembly. Several months ago, other researchers were the first to publish a draft genome of the red gromwell (i.e. Lithospermum erythrorhizon). However, we considered that the genome size they estimated and assembled was incorrect. This study meticulously estimated the L. erythrorhizon genome size to should be ∼708.74 Mb and further provided a reliable genome version (size ≈ 693.34 Mb; contigN50 length ≈ 238.08 Kb) to support our objection. Furthermore, according to our genome, we identified a gene family of the alkannin/shikonin O-acyltransferases (i.e. AAT/SAT) that catalysed enantiomer-specific acylations in the alkannin/shikonin biosynthesis (a characteristic metabolic pathway in L. erythrorhizon's roots) and further explored its evolutionary process. The results indicated that the existing AAT/SAT were not generated from only one round of gene duplication but three rounds; after different rounds of gene duplication, the existing AAT/SAT and their recent ancestors were under positive selection at different amino acid sites. These suggested that a combined power from gene duplication plus positive selection plausibly propelled AAT/SAT's functional differentiation in evolution.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9231145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Elucidation of the speciation history of three sister species of crown-of-thorns starfish (Acanthaster spp.) based on genomic analysis. 基于基因组分析的棘冠海星3个姊妹种的物种形成史研究。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-08-25 DOI: 10.1093/dnares/dsab012

Hideaki Yuasa, Rei Kajitani, Yuta Nakamura, Kazuki Takahashi, Miki Okuno, Fumiya Kobayashi, Takahiro Shinoda, Atsushi Toyoda, Yutaka Suzuki, Nalinee Thongtham, Zac Forsman, Omri Bronstein, Davide Seveso, Enrico Montalbetti, Coralie Taquet, Gal Eyal, Nina Yasuda, Takehiko Itoh

{"title":"Elucidation of the speciation history of three sister species of crown-of-thorns starfish (Acanthaster spp.) based on genomic analysis.","authors":"Hideaki Yuasa, Rei Kajitani, Yuta Nakamura, Kazuki Takahashi, Miki Okuno, Fumiya Kobayashi, Takahiro Shinoda, Atsushi Toyoda, Yutaka Suzuki, Nalinee Thongtham, Zac Forsman, Omri Bronstein, Davide Seveso, Enrico Montalbetti, Coralie Taquet, Gal Eyal, Nina Yasuda, Takehiko Itoh","doi":"10.1093/dnares/dsab012","DOIUrl":"https://doi.org/10.1093/dnares/dsab012","url":null,"abstract":"The crown-of-thorns starfish (COTS) is a coral predator that is widely distributed in Indo-Pacific Oceans. A previous phylogenetic study using partial mitochondrial sequences suggested that COTS had diverged into four distinct species, but a nuclear genome-based analysis to confirm this was not conducted. To address this, COTS species nuclear genome sequences were analysed here, sequencing Northern Indian Ocean (NIO) and Red Sea (RS) species genomes for the first time, followed by a comparative analysis with the Pacific Ocean (PO) species. Phylogenetic analysis and ADMIXTURE analysis revealed clear divergences between the three COTS species. Furthermore, within the PO species, the phylogenetic position of the Hawaiian sample was further away from the other Pacific-derived samples than expected based on the mitochondrial data, suggesting that it may be a PO subspecies. The pairwise sequentially Markovian coalescent model showed that the trajectories of the population size diverged by region during the Mid-Pleistocene transition when the sea-level was dramatically decreased, strongly suggesting that the three COTS species experienced allopatric speciation. Analysis of the orthologues indicated that there were remarkable genes with species-specific positive selection in the genomes of the PO and RS species, which suggested that there may be local adaptations in the COTS species.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386664/pdf/dsab012.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae. 感染临床病原体肺炎克雷伯菌的四种新型噬菌体的基因组特征。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-08-25 DOI: 10.1093/dnares/dsab013

Estrada Bonilla, Ana Rita Costa, Daan F van den Berg, Teunke van Rossum, Stefan Hagedoorn, Hielke Walinga, Minfeng Xiao, Wenchen Song, Pieter-Jan Haas, Franklin L Nobrega, Stan J J Brouns

{"title":"Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae.","authors":"Estrada Bonilla, Ana Rita Costa, Daan F van den Berg, Teunke van Rossum, Stefan Hagedoorn, Hielke Walinga, Minfeng Xiao, Wenchen Song, Pieter-Jan Haas, Franklin L Nobrega, Stan J J Brouns","doi":"10.1093/dnares/dsab013","DOIUrl":"https://doi.org/10.1093/dnares/dsab013","url":null,"abstract":"Bacteriophages are an invaluable source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses as biotechnological and medical tools, and help unravel the diversity of biological mechanisms employed by phages to take over the host during viral infection. Aiming to expand the available collection of phage genomes, we have isolated, sequenced, and assembled the genome sequences of four phages that infect the clinical pathogen Klebsiella pneumoniae: vB_KpnP_FBKp16, vB_KpnP_FBKp27, vB_KpnM_FBKp34, and Jumbo phage vB_KpnM_FBKp24. The four phages show very low (0-13%) identity to genomic phage sequences deposited in the GenBank database. Three of the four phages encode tRNAs and have a GC content very dissimilar to that of the host. Importantly, the genome sequences of the phages reveal potentially novel DNA packaging mechanisms as well as distinct clades of tubulin spindle and nucleus shell proteins that some phages use to compartmentalize viral replication. Overall, this study contributes to uncovering previously unknown virus diversity, and provides novel candidates for phage therapy applications against antibiotic-resistant K. pneumoniae infections.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4b/6f/dsab013.PMC8386662.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39309520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The complete mitogenome assemblies of 10 diploid potato clones reveal recombination and overlapping variants. 对10个二倍体马铃薯无性系的有丝分裂基因组进行了分析，发现了重组和重叠变异。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-08-25 DOI: 10.1093/dnares/dsab009

Sai Reddy Achakkagari, Helen H Tai, Charlotte Davidson, Hielke De Jong, Martina V Strömvik

{"title":"The complete mitogenome assemblies of 10 diploid potato clones reveal recombination and overlapping variants.","authors":"Sai Reddy Achakkagari, Helen H Tai, Charlotte Davidson, Hielke De Jong, Martina V Strömvik","doi":"10.1093/dnares/dsab009","DOIUrl":"https://doi.org/10.1093/dnares/dsab009","url":null,"abstract":"The potato mitogenome is complex and to understand various biological functions and nuclear-cytoplasmic interactions, it is important to characterize its gene content and structure. In this study, the complete mitogenome sequences of nine diploid potato clones along with a diploid Solanum okadae clone were characterized. Each mitogenome was assembled and annotated from Pacific Biosciences (PacBio) long reads and 10X genomics short reads. The results show that each mitogenome consists of multiple circular molecules with similar structure and gene organization, though two groups (clones 07506-01, DW84-1457, 08675-21 and H412-1 in one group, and clones W5281-2, 12625-02, 12120-03 and 11379-03 in another group) could be distinguished, and two mitogenomes (clone 10908-06 and OKA15) were not consistent with those or with each other. Significant differences in the repeat structure of the 10 mitogenomes were found, as was recombination events leading to multiple sub-genomic circles. Comparison between individual molecules revealed a translocation of ∼774 bp region located between a short repeat of 40 bp in molecule 3 of each mitogenome, and an insertion of the same in molecule 2 of the 10908-06 mitogenome. Finally, phylogenetic analyses revealed a close relationship between the mitogenomes of these clones and previously published potato mitogenomes.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39178506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Chromosome-scale genome assembly of the transformation-amenable common wheat cultivar 'Fielder'. 可转化普通小麦品种‘菲尔德’的染色体尺度基因组组装。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-06-25 DOI: 10.1093/dnares/dsab008

Kazuhiro Sato, Fumitaka Abe, Martin Mascher, Georg Haberer, Heidrun Gundlach, Manuel Spannagl, Kenta Shirasawa, Sachiko Isobe

{"title":"Chromosome-scale genome assembly of the transformation-amenable common wheat cultivar 'Fielder'.","authors":"Kazuhiro Sato, Fumitaka Abe, Martin Mascher, Georg Haberer, Heidrun Gundlach, Manuel Spannagl, Kenta Shirasawa, Sachiko Isobe","doi":"10.1093/dnares/dsab008","DOIUrl":"https://doi.org/10.1093/dnares/dsab008","url":null,"abstract":"We have established a high-quality, chromosome-level genome assembly for the hexaploid common wheat cultivar 'Fielder', an American, soft, white, pastry-type wheat released in 1974 and known for its amenability to Agrobacterium tumefaciens-mediated transformation and genome editing. Accurate, long-read sequences were obtained using PacBio circular consensus sequencing with the HiFi approach. Sequence reads from 16 SMRT cells assembled using the hifiasm assembler produced assemblies with N50 greater than 20 Mb. We used the Omni-C chromosome conformation capture technique to order contigs into chromosome-level assemblies, resulting in 21 pseudomolecules with a cumulative size of 14.7 and 0.3 Gb of unanchored contigs. Mapping of published short reads from a transgenic wheat plant with an edited seed-dormancy gene, TaQsd1, identified four positions of transgene insertion into wheat chromosomes. Detection of guide RNA sequences in pseudomolecules provided candidates for off-target mutation induction. These results demonstrate the efficiency of chromosome-scale assembly using PacBio HiFi reads and their application in wheat genome-editing studies.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9545065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle. 猪骨骼肌产前发育过程中染色质结构的重组。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-05-02 DOI: 10.1093/dnares/dsab003

Renqiang Yuan, Jiaman Zhang, Yujie Wang, Xingxing Zhu, Silu Hu, Jianhua Zeng, Feng Liang, Qianzi Tang, Yaosheng Chen, Luxi Chen, Wei Zhu, Mingzhou Li, Delin Mo

{"title":"Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle.","authors":"Renqiang Yuan, Jiaman Zhang, Yujie Wang, Xingxing Zhu, Silu Hu, Jianhua Zeng, Feng Liang, Qianzi Tang, Yaosheng Chen, Luxi Chen, Wei Zhu, Mingzhou Li, Delin Mo","doi":"10.1093/dnares/dsab003","DOIUrl":"https://doi.org/10.1093/dnares/dsab003","url":null,"abstract":"Myofibres (primary and secondary myofibre) are the basic structure of muscle and the determinant of muscle mass. To explore the skeletal muscle developmental processes from primary myofibres to secondary myofibres in pigs, we conducted an integrative three-dimensional structure of genome and transcriptomic characterization of longissimus dorsi muscle of pig from primary myofibre formation stage [embryonic Day 35 (E35)] to secondary myofibre formation stage (E80). In the hierarchical genomic structure, we found that 11.43% of genome switched compartment A/B status, 14.53% of topologically associating domains are changed intradomain interactions (D-scores) and 2,730 genes with differential promoter-enhancer interactions and (or) enhancer activity from E35 to E80. The alterations of genome architecture were found to correlate with expression of genes that play significant roles in neuromuscular junction, embryonic morphogenesis, skeletal muscle development or metabolism, typically, NEFL, MuSK, SLN, Mef2D and GCK. Significantly, Sox6 and MATN2 play important roles in the process of primary to secondary myofibres formation and increase the regulatory potential score and genes expression in it. In brief, we reveal the genomic reorganization from E35 to E80 and construct genome-wide high-resolution interaction maps that provide a resource for studying long-range control of gene expression from E35 to E80.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/dnares/dsab003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38997679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The Crown Pearl: a draft genome assembly of the European freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758). 王冠珍珠:欧洲淡水珍珠贻贝Margaritifera Margaritifera基因组汇编草图(林奈，1758)。

IF 4.1 2区生物学

DNA Research Pub Date : 2021-05-02 DOI: 10.1093/dnares/dsab002

André Gomes-Dos-Santos, Manuel Lopes-Lima, André M Machado, António Marcos Ramos, Ana Usié, Ivan N Bolotov, Ilya V Vikhrev, Sophie Breton, L Filipe C Castro, Rute R da Fonseca, Juergen Geist, Martin E Österling, Vincent Prié, Amílcar Teixeira, Han Ming Gan, Oleg Simakov, Elsa Froufe

{"title":"The Crown Pearl: a draft genome assembly of the European freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758).","authors":"André Gomes-Dos-Santos, Manuel Lopes-Lima, André M Machado, António Marcos Ramos, Ana Usié, Ivan N Bolotov, Ilya V Vikhrev, Sophie Breton, L Filipe C Castro, Rute R da Fonseca, Juergen Geist, Martin E Österling, Vincent Prié, Amílcar Teixeira, Han Ming Gan, Oleg Simakov, Elsa Froufe","doi":"10.1093/dnares/dsab002","DOIUrl":"https://doi.org/10.1093/dnares/dsab002","url":null,"abstract":"Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was produced using a combination of Illumina Paired-end and Mate-pair approaches. The genome assembly was 2.4 Gb long, possessing 105,185 scaffolds and a scaffold N50 length of 288,726 bp. The ab initio gene prediction allowed the identification of 35,119 protein-coding genes. This genome represents an essential resource for studying this species' unique biological and evolutionary features and ultimately will help to develop new tools to promote its conservation.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"28 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2021-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/dnares/dsab002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25507523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12