{"title":"Development and Content Analysis of Lubeg (Syzygium lineatum)","authors":"M. Baua","doi":"10.14719/pst.2351","DOIUrl":"https://doi.org/10.14719/pst.2351","url":null,"abstract":"The study discusses the development and composition analysis of Lubeg Vinegar. According to the Department of Agriculture Bureau of Agricultural Research, the lubeg fruit was previously an unutilized fruit. Since the fruit is easily accessible and often wasted, the researcher employed it as the primary ingredient in vinegar production. A total of 30 individuals from Isabela State University evaluated the Lubeg Vinegar. The research study utilized Mean (M) and Standard Deviation (SD). The acceptability and marketability scores of two samples of Lubeg (Syzygium lineatum) vinegar (brown sugar and white sugar) were compared using an independent samples t-test. The sizes were calculated using partial eta squared and interpreted according to Cohen's guidelines: 0.01 indicated a small effect, 0.06 a medium effect and 0.14 a large effect. Consequently, it became evident from the research that the method for making Lubeg vinegar from lubeg (Syzygium lineatum) was highly successful and efficient. Furthermore, lubeg (Syzygium lineatum) vinegar influenced consumer acceptability and was generally well-received by tasters. However, concerning color/appearance, aroma/smell, sour/acidity and respondents' willingness to purchase, Lubeg (Syzygium lineatum) vinegar with white sugar was generally more acceptable than Lubeg vinegar with brown sugar. Moreover, the commercialization of Lubeg vinegar has yielded a profitable return on investment.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"71 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaseela Valiya Thodiyil, Sinisha Edathumthazhe Kuni, Pradeep Nediyaparambu Sukumaran
{"title":"A modified CTAB method for extracting high-quality genomic DNA from aquatic plants","authors":"Jaseela Valiya Thodiyil, Sinisha Edathumthazhe Kuni, Pradeep Nediyaparambu Sukumaran","doi":"10.14719/pst.2850","DOIUrl":"https://doi.org/10.14719/pst.2850","url":null,"abstract":"This study introduces a streamlined approach for extractinghigh-quality DNA fromaquatic plants using CTAB, catering to molecular studies. Seven aquatic plant species(Hygrophilaauriculata, Limnophilarepens, Crinum malabaricum, Lagenandraovata, Ludwigiaperuviana, Eichhorniacrassipes, and Ipomoea aquatica) spanning six orderswere subjected toDNA extraction. The method combinesmechanical lysis and chemical treatments to effectively disrupt cells, coupled with RNase treatment and phenol extraction to mitigate RNA and protein contamination. The optimizedCTAB protocol facilitatesthe extraction of high-quality genomic DNA, suitable for amplifying plant barcode genes such as ITS and rbcL, as well asmarkerslike RAPD and ISSR, thereby enhancing the efficiency and reliability of genomic studies in aquatic plants.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"79 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amina Moutawalli, F. Benkhouili, Lahcen Ouchari, Elmostafa El Fahime, H. Benzeid, A. Doukkali, A. Zahidi
{"title":"Quantitative phytochemical, antioxidant and antimicrobial properties of the seeds of Lawsonia inermis L.","authors":"Amina Moutawalli, F. Benkhouili, Lahcen Ouchari, Elmostafa El Fahime, H. Benzeid, A. Doukkali, A. Zahidi","doi":"10.14719/pst.2834","DOIUrl":"https://doi.org/10.14719/pst.2834","url":null,"abstract":"Lawsonia inermis, commonly known as Henna, is a plant that has been extensively utilized throughout the ages for its medicinal and cosmetic properties. This plant contains a variety of bioactive compounds and has attracted significant interest due to its potential antioxidant and antimicrobial activities. The aim of our study is to evaluate the antioxidant capacity and antibacterial activity of Lawsonia inermis seeds. L. inermis seed powder was extracted using hexane, dichloromethane, ethanol and water. The 4 fractions were quantified for polyphenols, flavonoids and tannins contents. The antioxidant capacity was assessed using 4 methods: 2,2?-Diphenyl- picrylhydrazyl hydrate (DPPH) , 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic) (ABTS), Ferric Reducing Antioxidant Power (FRAP), and Phosphomolybdate assay (PMA). Antimicrobial activity was determined by agar diffusion against Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The liquid dilution method was used to determine the minimum inhibitory concentration, minimum bactericidal concentration and minimum fungicidal concentration. The highest content of polyphenols and tannins was obtained in the ethanolic fraction, with values of 594.01±6.07 mg GAE/g dw and 242.4±1.6 mg CE/g dw respectively. The ethanolic fraction exhibited significant scavenging of free radicals (DPPH) with an IC50 of 3.06±0.01 µg/mL, lower than the IC50 of the standard quercetin (5.30±0.02 µg/mL). Moreover, the ethanolic fraction showed antimicrobial activity against S. aureus, B. cereus and E. faecalis strains, with zone of inhibition values of 15.5±0.7, 14.0±0.0 and 14.5±0.7 mm respectively. The minimum inhibitory concentration of the ethanolic fraction was found to be 1.563 mg/mL against both S. aureus and B. cereus strains, while the minimum bactericidal concentration was 3.125 mg/mL against B. cereus and 6.25 mg/mL against E. faecalis strains. L. inermis seeds exhibit significant antioxidant capacity and demonstrate antimicrobial activity. These findings indicate the potential of L. inermis seeds as a valuable source of bioactive compounds with antioxidant and antimicrobial properties.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"31 28","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139962345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diversity and Chorotype Analysis of the Pteridophytes of Zimbabwe","authors":"A. Maroyi","doi":"10.14719/pst.3052","DOIUrl":"https://doi.org/10.14719/pst.3052","url":null,"abstract":"In the current investigation, an attempt has been made to document the floristic diversity and chorotype analysis of Pteridophytes growing in Zimbabwe. Previously published literature and online sources were consulted to confirm the nomenclature, precise locations, chorotype, stages of invasion, and conservation status of the Pteridophytes in Zimbabwe. The updated list of Pteridophyte taxa confirms that there are 297 taxa belonging to 83 genera and 19 families. The largest family is Aspleniaceae, with 78 taxa (26.26%), followed by Polypodiaceae and Pteridaceace with 67 taxa (22.56%) and 54 taxa (18.18%), respectively. The largest genus is Asplenium L., with 46 taxa (15.49%), followed by Hemionitis L. with 22 taxa (7.41%), Ophioglossum L. (16 taxa, 5.39%), Thelypteris Schmidel (14 taxa, 4.71%), and Elaphoglossum Schott ex J.Sm. (12 taxa, 4.04%). A total of 20 taxa (6.73%) are threatened with extinction, emphasizing the need for detailed knowledge of the nomenclature, conservation status, and distribution of Pteridophytes taxa in Zimbabwe. Research focusing on the diversity, ecology, and conservation status of Pteridophytes is imperative for biodiversity conservation, as they are an important component of natural ecosystems adapted to different habitats. Therefore, future studies on Pteridophytes in Zimbabwe should explore how the lack of floristic data on the plant group can hinder ecological research.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"23 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of different factors on in vitro multiplication and rooting of three local Juglans regia L. genotypes in Uzbekistan","authors":"Guljakhon Eshbekova, Ilyos Haydarov, Bakhtiyor Kadirov, Zafar Ismailov","doi":"10.14719/pst.2915","DOIUrl":"https://doi.org/10.14719/pst.2915","url":null,"abstract":"The Persian walnut (Juglans regia L.) is one of the most lucrative and widely distributed nut crops. It is appreciated as a forestry and ornamental tree in addition to its benefits as a fruit crop. Although Central Asian countries, especially Uzbekistan, are among the origins of the Persian walnut; they are not considered as top industrial producers of walnuts. Uzbekistan possesses a wide range of walnut genetic resources and as a result of conducted research, several promising, fruitful, early-harvesting varieties and forms have been selected. The aim of this study is to optimize microclonal in vitro propagation of selected Uzbekistan local varieties and forms by evaluating concentrations of different plant growth regulators and genotype on multiplication and rooting stages. As mother plants, 2 forms and one variety were selected: the Ideal variety, 'Form PDM23' and 'Form 202YaKT'. ?n the proliferation stage, the growth rate of walnut microshoots on basal medium Driver and Kuniyuki Walnut Medium (DKW) with different concentrations of 6-benzylaminopurine (BAP) and Indole-3-butyric acid (IBA) (0.01 mg/L) was studied. The rooting stage was assessed in half strength macronutrient DKW medium containing different IBA concentrations (0.0, 2.0, 4.0 and 6.0 mg/L). The Ideal variety and 'Form PDM23' performed best in DKW medium supplied with 1.5 mg/L BAP and 0.01 mg/L IBA, whereas 'Form 202YaKT' performed best in DKW medium supplemented with 1.0 mg/L BAP and 0.01 mg/ L IBA for the proliferation stage. For all genotypes, 6.0 mg/L IBA provided the best rooting results.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"30 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139962243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. AL-Taie, N. K. Al-Zubaidi, A. Matrood, A Rhouma
{"title":"Role of plant growth promoting fungi and doses of chemical fertilizers in improving agronomic response for sustainable wheat crop production","authors":"A. H. AL-Taie, N. K. Al-Zubaidi, A. Matrood, A Rhouma","doi":"10.14719/pst.2052","DOIUrl":"https://doi.org/10.14719/pst.2052","url":null,"abstract":"Plant growth promoting fungi PGPF is considered a major biological technique that results in significant plant growth and yield. Use of high doses of chemical fertilizers increased cost of crop production and environmental pollution. PGPF could be a reliable alternative to reduce application of agrochemicals such as chemical fertilizers in modern agriculture. The present study evaluated the role of these fungi with chemical fertilizer of DAP applied through different doses in agronomic response of production of 2 varieties of Wheat (Ibaa99 and Rasheed) at 2 agricultural seasons 2019-2020-2021. Field plots conducted during 2019-2020 cropping season, to evaluate the role of adding fungal isolate called PGPF-AT with doses of DAP were (0, 50 and 100%) in 0, 120 and 240 Kg ha-1 respectively, compared with (Commercial Biofertilizer) called Natrusoil as positive control on wheat var. Ibaa99 to improve growth and yield attributes. The study was repeated in the 2020-2021 cropping seasons with var. Rasheed (to discover a variety of effects and confirm results). The results revealed that treatment PGPF-AT was exerted a highly significant increase without differences between doses of DAP 120, and 240 Kg ha-1 compared to untreated plots for both Ibaa 99 and Rasheed varieties attributes: no. of plants m-2, plant height (cm), plant dry weight (g), no. of tillers plant-1, no. of spike plant-1, spike length (cm), spike weight (g), no. of seeds spike-1, weight of 100 grain (g) and grain yield Kg m-2 in addition to fertilizer use efficiency (Kg Kg-1) in both cropping seasons.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"36 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139961892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Citrus for wellness: Exploring the bioactive properties of Citrus medica fruit peel with emphasis on its anticancer, antioxidant, antimicrobial and anthelmintic properties","authors":"Saranya Jayaram, Suma Sarojini, Sriganesh Bangalore Anand, Anto Akil Irudaya Raj, Anju Parakadan, Indhu Philip, Soma Biswas","doi":"10.14719/pst.2612","DOIUrl":"https://doi.org/10.14719/pst.2612","url":null,"abstract":"Citrus medica (Citron) is an underutilised plant consisting of various bioactive elements with numerous medicinal benefits. The present study aimed to evaluate the bioactive properties, including anthelmintic, antimicrobial, antioxidant and anticancer activities, of chloroform extract obtained from the of fruit peel of C. medica. The different types of phytochemicals present in the chloroform extract were analysed using GC-MS. The major components detected included n-hexadecanoic acid, octadecanoic acid, t-tetradecenal, 1-nonadecene etc. Anthelmintic study was conducted using Eisenia fetida as a test organism, revealing a significant anthelmintic effect in the C. medica fruit peel extract compared to the standard drug. Antimicrobial properties were assessed against five test bacterial and fungal strains. Antibacterial tests showed zones of inhibition ranging from 8 to 11 mm, while no prominent zones of inhibition were observed in antifungal tests. The DPPH assay demonstrated significant antioxidant properties of Citron fruit peel extract compared to the standard ascorbic acid. The Chloroform extract of citron fruit peel exhibited significant cytotoxic properties against FaDu (human hypopharyngeal tumour) cell line. The present study indicates the potential of the chloroform extract of C. medica fruit peel to be employed as an anthelmintic, antibacterial, antioxidant and anticancer agent. Hence, it emphasises the prominence that can be given to the dietary consumption of citrus fruit peel in various forms, such as dried peel, powder etc.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"10 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139523751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadya Sofia Siti Sa'adah, S. Sukirno, Tri Rini Nuringtyas, L. H. Nugroho
{"title":"Toxicity of Ocimum basilicum L. leaf extract against Spodoptera exigua Hübner (Lepidoptera: Noctuidae)","authors":"Nadya Sofia Siti Sa'adah, S. Sukirno, Tri Rini Nuringtyas, L. H. Nugroho","doi":"10.14719/pst.3099","DOIUrl":"https://doi.org/10.14719/pst.3099","url":null,"abstract":"The beet armyworm Spodoptera exigua Hübner (Lepidoptera: Noctuidae) is one of the most significant shallot pests worldwide, which affects agricultural production by approximately 100% in Indonesia. The growing awareness of sustainable agriculture encourages the search for natural alternatives to synthetic pesticides that promote resistance and contaminate the environment. Therefore, this study aims to evaluate the efficacy of basil (Ocimum basilicum L) leaf extract against the 2nd and 3rd instar larvae of S. exigua. The extracts were prepared using maceration and hydrodistillation and the pathogenicity was carried out against the 2nd and 3rd instar of S. exigua using three replicates with several doses of extract ranging from 0- 2.0%. The extracts were applied to the larvae using contact and topical methods. The results showed that maceration extraction yields a greater extract with different types compared to those of the hydrodistillation method. Maceration extract of basil leaves with the contact application method to 2nd instar showed better results than the topical application with the highest mortality rate of 75% (F=24.464; P< 0.001) and LC50 of 0.007%. This indicated that basil leaf extract with the contact application method has great potential to be developed as a botanical insecticide to control S. exigua in the field as part of Integrated Pest Management (IPM).","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"2 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139524675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diachronic study of the floristic diversity of the Royal Mausoleum of Mauretania, Algeria","authors":"Badia Siab-Farsi, Yamina Kadid, Chafika Bouzahar","doi":"10.14719/pst.2819","DOIUrl":"https://doi.org/10.14719/pst.2819","url":null,"abstract":"This study aimed to provide knowledge on the mural flora of the Royal Mausoleum of Mauretania. Through floristic surveys, we complied a catalog listing168 vascular plants belonging to 141 genera and 58 families. Asteraceae and Poaceae were the most dominant families, constituting 24.81% of the species. Dominant characteristics of this flora included therophytes (41.07%) and Mediterranean floristic elements (67.26%). To update the floristic list of the Mausoleum, we compared our data with 3 old lists from 1867, 1928 and 1985. The diachronic analysis reveals the persistance of 35 taxa representing 16.27% of 215 taxa listed since the first inventory, and the emergence of 30 new taxa (13.95%). The diversity of this wall flora is associated with changes in the landscape around the Mausoleum as well as its conservation status.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"5 34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139439757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sheily Nneka Egonu, Uche Cyprian Okafor, Chinyere Chioma Isuosuo, Obiora Emmanuel Udoh, Chukwuma Kenechukwu Chukwuemeka, Emmanuel Gabriel Njoku, O. S. Udengwu
{"title":"EVALUATING THE PRODUCTION POTENTIAL OF SIX (6) COMBRETUM SPECIES IN THE BIOMIMETIC SYNTHESIS OF SILVER NANOPARTICLES","authors":"Sheily Nneka Egonu, Uche Cyprian Okafor, Chinyere Chioma Isuosuo, Obiora Emmanuel Udoh, Chukwuma Kenechukwu Chukwuemeka, Emmanuel Gabriel Njoku, O. S. Udengwu","doi":"10.14719/pst.2489","DOIUrl":"https://doi.org/10.14719/pst.2489","url":null,"abstract":"Existing research has shown that plants are best suited for the ecofriendly green synthesis of nanoparticles. This study reported novel findings on the biomimetic synthesis of silver nanoparticles using the aqueous leaf extract of six medicinal plant species in the genus, Combretum (C. paniculatum, C. dolichopetalum, C. platypterum, C racemosum, C. bauchiense, and C. demeusei). It was concerned with the characterization, phytochemical screening, antimicrobial and cytological evaluation of the synthesized silver nanoparticles (AgNPs). The novelty of this study lies in the creation of a nanoparticle production index (NPPI) of the species based on their crystalline sizes (in nm) and yield (in mg/l) of AgNPs. This NPPI can be defined as a measure of the quantity of particles produced with respect to the experimental species. The study also investigated whether these species would produce similar results since they belong to the same genus. It was observed that the smaller the size of the nanoparticles, the higher the production index. The highest production index was observed in C. paniculatum (18.75 nm, 144. 6 mg/l), and the least in C. demeusei (0 nm, 6.3 mg/l). The AgNPs were characterized using various standard analytical techniques. The X-ray diffraction analysis indicated that the species showed 2 theta values in the 40° range, all corresponding to miller indices of (200). The synthesized AgNPs showed varying but significant antimicrobial activities. Also, majority of the species showed a high NPPI. The study heralds a system, “phytonanotaxonomy”, the classification of plants of the same taxa based on their NPPI.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"9 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139534283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}