Jaseela Valiya Thodiyil, Sinisha Edathumthazhe Kuni, Pradeep Nediyaparambu Sukumaran
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引用次数: 0
摘要
本研究介绍了一种利用 CTAB 从水生植物中提取高质量 DNA 的简化方法，以满足分子研究的需要。研究人员对横跨六纲的七种水生植物（Hygrophilaauriculata、Limnophilarepens、Crinum malabaricum、Lagenandraovata、Ludwigiaperuviana、Eichhorniacrassipes 和 Ipomoea aquatica）进行了 DNA 提取。该方法结合了机械裂解和化学处理，可有效破坏细胞，并通过 RNase 处理和苯酚提取减少 RNA 和蛋白质污染。优化后的 CTAB 方案有助于提取高质量的基因组 DNA，适用于扩增 ITS 和 rbcL 等植物条形码基因以及 RAPD 和 ISSR 等标记物，从而提高了水生植物基因组研究的效率和可靠性。
A modified CTAB method for extracting high-quality genomic DNA from aquatic plants
This study introduces a streamlined approach for extractinghigh-quality DNA fromaquatic plants using CTAB, catering to molecular studies. Seven aquatic plant species(Hygrophilaauriculata, Limnophilarepens, Crinum malabaricum, Lagenandraovata, Ludwigiaperuviana, Eichhorniacrassipes, and Ipomoea aquatica) spanning six orderswere subjected toDNA extraction. The method combinesmechanical lysis and chemical treatments to effectively disrupt cells, coupled with RNase treatment and phenol extraction to mitigate RNA and protein contamination. The optimizedCTAB protocol facilitatesthe extraction of high-quality genomic DNA, suitable for amplifying plant barcode genes such as ITS and rbcL, as well asmarkerslike RAPD and ISSR, thereby enhancing the efficiency and reliability of genomic studies in aquatic plants.
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