HAYATI Journal of Biosciences最新文献_第2页

Aromatherapeutic Antibacterials: Comparative Study of 40 Essential Oils and Their Biofilm Inhibition in Pseudomonas aeruginosa ATCC9027 芳香治疗抗菌剂：40 种精油及其对铜绿假单胞菌 ATCC9027 生物膜抑制作用的比较研究

HAYATI Journal of Biosciences Pub Date : 2024-01-16 DOI: 10.4308/hjb.31.3.474-485

Nur Aziz, Rollando, Eva Monica, F.X Haryanto Susanto

{"title":"Aromatherapeutic Antibacterials: Comparative Study of 40 Essential Oils and Their Biofilm Inhibition in Pseudomonas aeruginosa ATCC9027","authors":"Nur Aziz, Rollando, Eva Monica, F.X Haryanto Susanto","doi":"10.4308/hjb.31.3.474-485","DOIUrl":"https://doi.org/10.4308/hjb.31.3.474-485","url":null,"abstract":"Growing evidence suggests that biofilm formation in Pseudomonas aeruginosa (PA) results from quorum sensing, reducing bacterial sensitivity to antibiotics and fostering the development of antibiotic-resistant strains. Addressing the need for a compound that can inhibit quorum sensing signals to mitigate biofilm formation in PA, this study screened 40 essential oils for their potential as quorum sensing inhibitors against PA ATCC9027. Utilizing the agar well diffusion assay, the antibacterial activity of these essential oils was compared with levofloxacin, revealing that 25 out of 40 essential oils exhibited antibacterial activity against PA ATCC9027. The highest antibacterial activity was exhibited by essential oils from Cinnamomum burmannii (Cinnamon Bark Oil), Cananga odorata (Ylang-ylang Oil), and Eucalyptus globulus (Eucalyptus Oil), confirmed by respective MIC values of 0.09765% (v/v), 0.390625% (v/v), and 1.5625% (v/v), and MBC values of 0.09765% (v/v), > 1.5625% (v/v), and > 1.5625% (v/v). Growth curve inhibition assay followed by crystal violet biofilm formation assay revealed that only Ylang-ylang Oil exhibited biofilm formation inhibition at 1/4 MIC concentration. Docking analysis of compounds from these essential oils against quorum sensing enzymes (LasR, PqsR, and QscR) revealed that Ylang-ylang Oil compounds exhibited the highest binding affinity. In conclusion, among the 40 essential oils tested, 25 show potential as antibacterials against PA ATCC9027, with Ylang-ylang Oil standing out as a promising candidate for quorum sensing inhibitor development. The use and development of quorum sensing inhibitors from Ylang-ylang Oil compounds are expected to reduce the burden of antibiotic resistance, especially in Pseudomonas aeruginosa.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"57 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of 17 Genotypes of Taro Based on Anatomy and Nutritional Value of Tuber 基于解剖和块茎营养价值的 17 种芋头基因型的多样性

HAYATI Journal of Biosciences Pub Date : 2024-01-09 DOI: 10.4308/hjb.31.3.465-473

L. Cahyanti, D. Sopandie, E. Santosa, Heni Purnamawati

引用次数: 0

Parang Romang (Boehmeria virgata (Frost.) Guill.): Correlation of Phytochemistry with Antioxidant and Xanthine Oxidase Inhibitory Activities Parang Romang（Boehmeria virgata (Frost.) Guill.）：植物化学与抗氧化和黄嘌呤氧化酶抑制活性的相关性

HAYATI Journal of Biosciences Pub Date : 2024-01-08 DOI: 10.4308/hjb.31.3.457-464

Muh Ikhlas Arsul, I. Fidrianny, M. Insanu

{"title":"Parang Romang (Boehmeria virgata (Frost.) Guill.): Correlation of Phytochemistry with Antioxidant and Xanthine Oxidase Inhibitory Activities","authors":"Muh Ikhlas Arsul, I. Fidrianny, M. Insanu","doi":"10.4308/hjb.31.3.457-464","DOIUrl":"https://doi.org/10.4308/hjb.31.3.457-464","url":null,"abstract":"Secondary metabolites such as phenolic and flavonoid from the plant are essential in their activity, especially activities related to the prevention of oxidation and the inhibition of xanthine oxidase. One plant that is believed to have prevention of oxidation and XOI and correlates with secondary metabolites is parang romang. The purpose of this study is to assess the antioxidant capacity and xanthine oxidase inhibitory activities of parang romang, and to examine their relationship with the TPC and TFC. Total phenolic and flavonoid were measured for all parts, and the DPPH, FRAP, and CUPRAC methods determined antioxidant capacity. The activity of xanthine oxidase inhibition was also assessed. Flavonoids, alkaloids, and coumarin were found in parang romang. The steroid/triterpenoid was found in the stem, leaves, and flower; saponin was in the roots and flower; quinone and tannin were only in the roots. The root gave the highest of TPC, while the flower had the highest TFC. The highest antioxidant capacity was found in roots, leaves, flowers, and stems. The highest xanthine oxidase inhibitory was given by leaves (9.74±0.14 µg/ml), followed by flower, steam, and rood. The correlation was shown between the phenolic and flavonoid quantities and the actions of antioxidants and xanthine oxidase inhibitors.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"23 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140512669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Isolation of Endophytic Fungi from Rui (Harrisonia perforata (Blanco) Merr.) and Determining Their Antibacterial, Antioxidant, and Cytotoxic Activity 从瑞木（Harrisonia perforata (Blanco) Merr.）中分离内生真菌并测定其抗菌、抗氧化和细胞毒性活性

HAYATI Journal of Biosciences Pub Date : 2024-01-08 DOI: 10.4308/hjb.31.3.443-456

S. Anam, Armini Syamsidi, Muh. Fadil Usman Tunreng, Helena Fransisca Djaleha, Windhy Nur Arisca, Gunawan Syaputra, Iklima, Meri Feki Indriani, Agustinus Widodo, Ritha Pratiwi, Yuliet, Abd. Rahman Razak

{"title":"Isolation of Endophytic Fungi from Rui (Harrisonia perforata (Blanco) Merr.) and Determining Their Antibacterial, Antioxidant, and Cytotoxic Activity","authors":"S. Anam, Armini Syamsidi, Muh. Fadil Usman Tunreng, Helena Fransisca Djaleha, Windhy Nur Arisca, Gunawan Syaputra, Iklima, Meri Feki Indriani, Agustinus Widodo, Ritha Pratiwi, Yuliet, Abd. Rahman Razak","doi":"10.4308/hjb.31.3.443-456","DOIUrl":"https://doi.org/10.4308/hjb.31.3.443-456","url":null,"abstract":"Endophytic fungi produce bioactive metabolites that exhibit antibacterial, antioxidant, and anti-cancer activity. Rui (Harrisonia perforata (Blanco) Merr.) is a traditional medicinal plant proven to be an antibacterial and anticancer. Endophytic fungi live in plant tissues without damaging or producing chemicals that infect the host cell. Endophytes produce the same and similar compound as an original plant. This study aimed to isolate the endophytic fungi from Rui and determine their antibacterial, antioxidant, and cytotoxic activity. 13 fungal isolates were successfully isolated using PDA for seven days at 30°C. Molecular identification using the ITS1 and ITS4 DNA sequences only revealed six species: Diaporthe sp., Phomopsis sp., Aspergillus tubingensis, Aspergillus viridinutans, Pseudofusicoccum sp., and Daldinia eschscholtz. Seven isolates showed antibacterial activity based on plate assay. Aspergillus tubingensis and RA-1 exhibited antibacterial activity against Staphylococcus aureus and Salmonella typhi, with the MIC and MBC starting from 24 to 32 mg/ml. The DPPH assay showed the most increased antioxidant activity in Daldinia eschscholtzii with an IC50 of 98.14±2.39 µg/ml. The Brine Shrimp Lethality Test (BSLT) showed the highest potential cytotoxic activity, as shown by Aspergillus tubingensis and Phomopsis sp. with LC50 of 7.78±7.48 and 30.83±0.39 µg/ml. In conclusion, our study demonstrates that the fungal extract from Rui could be a source of antibacterial, antioxidant, and cytotoxic agents.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"9 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140512037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer 从棕榈油废料中分离出的可产生脂肪酶的 Lysinibacillus sphaericus

HAYATI Journal of Biosciences Pub Date : 2024-01-05 DOI: 10.4308/hjb.31.3.425-431

Sri Sumarsih, Sofijan Hadi, Fatimah, Rizka Diah Fitri, Gilva Illavi

引用次数: 0