Sri Sumarsih, Sofijan Hadi, Fatimah, Rizka Diah Fitri, Gilva Illavi
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引用次数: 0
摘要
本研究从棕榈油废料中分离出溶脂菌，用于生产脂肪酶。根据其形态和 16 rRNA 基因序列，确定了潜在脂肪分解细菌的种类。细菌在含有各种植物油和氮源的培养基中生长，从而产生酶。细菌产生的酶测定了其对底物对硝基苯棕榈酸酯的脂肪分解活性。根据形态学和系统发育分析，确认该脂肪分解菌为 Lysinibacillus sphaericus L49a。以不同植物油为碳源和不同氮源的矿物培养基适合于 L. sphaericus L49a 的生长和脂肪酶的产生。在含有硫酸铵和橄榄油的培养基中培养 L. sphaericus L49a 产生的脂肪酶具有最高的脂肪分解活性。
Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer
In this research, lipolyic bacteria have been isolated from palm oil waste land for the production of lipase. Species of potential lipolytic bacteria were identified based on their morphology and sequences of 16 rRNA gene. Enzymes are produced by growing bacteria in a medium with various vegetable oils and nitrogen sources. The enzyme produced by the bacteria measured its lipolytic activity against the substrate para-nitrophenylpalmitate. The lipolytic bacteria was recognized as Lysinibacillus sphaericus L49a based on morphological and phylogenetic analysis. Mineral media with different vegetable oils as carbon sources, and different nitrogen sources were suitable for growth and production of lipase enzymes of L. sphaericus L49a. Culltivation of L. sphaericus L49a in medium containing ammonium sulfate and olive oil produced lipase with the highest lipolytic activity.
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