HAYATI Journal of Biosciences最新文献

Effect of Toxoplasma Infection Dosage on Igg, Igm, Fetus Weight and Body Length, and Necrosis of Placenta and Fetal Heart 弓形虫感染剂量对 Igg、Igm、胎儿体重和身长以及胎盘和胎儿心脏坏死的影响

HAYATI Journal of Biosciences Pub Date : 2024-02-15 DOI: 10.4308/hjb.31.3.596-602

Hafi Nurinasari, Sajidan, Bambang Purwanto, D. Indarto, D. Subekti

{"title":"Effect of Toxoplasma Infection Dosage on Igg, Igm, Fetus Weight and Body Length, and Necrosis of Placenta and Fetal Heart","authors":"Hafi Nurinasari, Sajidan, Bambang Purwanto, D. Indarto, D. Subekti","doi":"10.4308/hjb.31.3.596-602","DOIUrl":"https://doi.org/10.4308/hjb.31.3.596-602","url":null,"abstract":"One of the most prevalent zoonotic illnesses in the world, toxoplasmosis, affects both humans and animals and is caused by the parasite Toxoplasma gondii. The infection will trigger the immune system to increase antibody production. This study aims to determine the dose of toxoplasma that causes necrosis in rats placental and fetal hearts. This study was laboratory experimental research with a Randomized Control Trial (RCT). The study design used a post-test only with a control group design. The Ig G variable obtained from the control group (CG) and treatment group 3 (TG3) is the most significant because the mean value difference was the largest (176.56). The Ig M variable obtained from the control group with treatment group 3 (TG3) is the most significant because the mean value difference is the largest (33.47). The fetus weight variable obtained from the control group with treatment group 3 (TG3) is the most significant because the mean value difference is the largest (2.6). The body length variable obtained from the control group between treatment group 3 (TG3) is the most significant because the mean value difference is the largest (1.26). There was a significant difference in placental tissue necrosis (p = 0.034) and heart (p = 0.025) between the control group (CG) and treatment group 3 (TG3). Therefore, there was a significant difference in Ig G, Ig M, fetus weight, body length, placental tissue necrosis, and fetal heart at the 10³ dose compared to the 102, 101, and normal doses.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"286 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140455656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mangroves in Alas Purwo National Park, Indonesia: Diversity and Its Potential Carbon Services 印度尼西亚 Alas Purwo 国家公园的红树林：多样性及其潜在的碳服务

HAYATI Journal of Biosciences Pub Date : 2024-02-13 DOI: 10.4308/hjb.31.3.589-595

Seftiawan Samsu Rijal, M. T. Furqon, Muammar Kadhafi, Febriyani Eka Supriatin, Riqki Yoga Aprillianto

引用次数: 0

Improvement of Plasmid Volumetric Yield by Addition of Glycerol and Phosphate Buffer in Escherichia coli TOP10 Batch Culture 在大肠杆菌 TOP10 批次培养中添加甘油和磷酸盐缓冲液提高质粒体积产量

HAYATI Journal of Biosciences Pub Date : 2024-02-05 DOI: 10.4308/hjb.31.3.572-580

Anindyajati, Salma Aulia Afifah, C. Riani, Marselina Irasonia Tan, Dessy Natalia, E. Giri-Rachman, A. Artarini

{"title":"Improvement of Plasmid Volumetric Yield by Addition of Glycerol and Phosphate Buffer in Escherichia coli TOP10 Batch Culture","authors":"Anindyajati, Salma Aulia Afifah, C. Riani, Marselina Irasonia Tan, Dessy Natalia, E. Giri-Rachman, A. Artarini","doi":"10.4308/hjb.31.3.572-580","DOIUrl":"https://doi.org/10.4308/hjb.31.3.572-580","url":null,"abstract":"The investigation of mRNA development has gained substantial interest, particularly in the ex vivo and in vivo therapy. mRNA is widely used for the development of gene editing-based therapies and mRNA vaccines. The aim of this study was to optimize the medium and harvest time to increase plasmid DNA production as part of mRNA production. This study modified used a medium modification approach to achieve high density culture of Escherichia coli TOP10 pGEMT-N in batch cultivation method. Various media formulations were assessed, including LB; LB with phosphate buffer (K2HPO4 12.549 g/L and KH2PO4 2.31 g/L); LB with glycerol (50 g/L); LB with glycerol and phosphate buffer; LB with phosphate buffer, glycerol, glucose (15 g/L), and galactose (15 g/L). The effect of additional carbon sources and phosphate buffer on culture density was measured through OD600 and wet cell weight analysis. The highest OD600 and wet cell weight was observed when LB with glycerol and phosphate buffer was used, with OD600 of 4.78±0.14 and wet cell weight of 36.00±0.63 mg/ml. Plasmid DNA was subsequently isolated from these cultures following 5- and 7.5-hour incubation periods. The utilization of LB medium with glycerol and phosphate buffer resulted in a substantial increase in the volumetric concentration of plasmid DNA of 1,516.97±385.00 ng/ml after 5 hours of incubation. In conclusion, a remarkable enhancement in plasmid DNA volumetric yield within 5 hours was achieved by addition of glycerol and phosphate buffer to LB medium, leading to incubation period.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"302 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140461507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Drought Stress on Proline Gene Expression, Enzyme Activity, and Physiological Responses in Thai Mulberry (Morus spp.) 干旱胁迫对泰国桑树（桑属）脯氨酸基因表达、酶活性和生理反应的影响

HAYATI Journal of Biosciences Pub Date : 2024-02-02 DOI: 10.4308/hjb.31.3.559-571

Surapa Nutthapornnitchakul, W. Sonjaroon, Nutdanai Putthisawong, Natnicha Thumthuan, P. Tasanasuwan, C. Jantasuriyarat

{"title":"Effect of Drought Stress on Proline Gene Expression, Enzyme Activity, and Physiological Responses in Thai Mulberry (Morus spp.)","authors":"Surapa Nutthapornnitchakul, W. Sonjaroon, Nutdanai Putthisawong, Natnicha Thumthuan, P. Tasanasuwan, C. Jantasuriyarat","doi":"10.4308/hjb.31.3.559-571","DOIUrl":"https://doi.org/10.4308/hjb.31.3.559-571","url":null,"abstract":"Mulberries are vital for the silk industry as the sole natural food for silkworms, but their quality and quantity can be greatly impacted by environmental factors, notably water shortages or droughts. In this study, the proline content and expression levels of the Pyrroline-5-carboxylate reductase (P5CR) gene in four recommended Thai mulberry varieties (Sakhonnakorn, Sakhonnakorn 85, Burirum 60, and Srisaket 84) and one standard drought tolerant variety (SRCM9809-34) were measured under drought stress. Additionally, physiological data and antioxidative enzymatic activities were also examined. The findings revealed that SRCM9809-34, a drought-tolerant variety, had the lowest proline content, followed by Sakhonnakorn 85, Burirum 60, while the highest proline content was observed in Srisaket 84. Although there was no correlation between the expression level of the P5CR gene and proline content, the overall trend in all varieties was the same: proline content increased after drought conditions. Regarding physiological responses, the wilting score showed similar results to proline content, with SRCM9809-34 having the lowest proline content and wilting score. Moreover, SRCM9809-34 exhibited the highest RWC, Pn and WUE values, as well as the lowest level of MDA and H2O2. Our results validated and indicated that SRCM9809-34 is a drought-tolerant variety. From this finding, among the four Thai mulberry varieties, Sakhonnakorn 85 exhibited the highest potential for drought tolerance, and this potential can be enhanced through crossbreeding with SRCM9809-34.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"85 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140461997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Structure of Gallus varius Based on Middle-Lower Section of Control Region mtDNA 基于控制区 mtDNA 中下段的 Gallus varius 遗传结构

HAYATI Journal of Biosciences Pub Date : 2024-01-31 DOI: 10.4308/hjb.31.3.548-558

Alfiyan Achmad, Achmad Farajallah, Maria Ulfah, Dyah Perwitasari-Farajallah, M. Muladno

{"title":"Genetic Structure of Gallus varius Based on Middle-Lower Section of Control Region mtDNA","authors":"Alfiyan Achmad, Achmad Farajallah, Maria Ulfah, Dyah Perwitasari-Farajallah, M. Muladno","doi":"10.4308/hjb.31.3.548-558","DOIUrl":"https://doi.org/10.4308/hjb.31.3.548-558","url":null,"abstract":"Gallus varius, an indigenous bird species of Indonesia, demonstrates significant genetic diversity. The genetic diversity assessment in G. varius encompassed both mitochondrial DNA (mtDNA) and nuclear DNA, with the mtDNA analysis primarily centered on domain I of the control region. This study aimed to describe the genetic diversity and structure of G. varius inhabiting Java, Madura, Lombok and Sumbawa based on the middle-lower part of control region mtDNA. Genomic DNA was extracted from the calamus tip of feather, then the middle-lower part of control region was amplified and sequenced using two pairs of primers. In the examined control region, spanning from 944 to 1,008 bp, 13 bp of nucleotide variation was observed, with nucleotide diversity at 0.0021. Between G. varius samples and the reference (NC_007238.1), a total of 7 haplotypes were identified, 5 unique and 2 shared haplotypes, with haplotype diversity at 0.7692. The substantial diversity of haplotypes in this study and two previous study suggests that the genetic diversity of G. varius has remained stable over the past two decades. Additionally, genetic distance data indicate there is multiple G. varius subspecies, and the haplotype network accentuates signs of population differentiation.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"329 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiversity of Mangrove Brachyuran Crabs of Family Ocypodidae and Sesarmidae in Koto XI Tarusan District, West Sumatera, Indonesia 印度尼西亚西苏门答腊省 Koto XI Tarusan 地区 Ocypodidae 和 Sesarmidae 科红树林腕足蟹的生物多样性

HAYATI Journal of Biosciences Pub Date : 2024-01-29 DOI: 10.4308/hjb.31.3.507-516

Eni Kamal, Yuspardianto, Dwieke Putri Wulandari, Fitriyani, Amelia Sriwahyuni Lubis

{"title":"Biodiversity of Mangrove Brachyuran Crabs of Family Ocypodidae and Sesarmidae in Koto XI Tarusan District, West Sumatera, Indonesia","authors":"Eni Kamal, Yuspardianto, Dwieke Putri Wulandari, Fitriyani, Amelia Sriwahyuni Lubis","doi":"10.4308/hjb.31.3.507-516","DOIUrl":"https://doi.org/10.4308/hjb.31.3.507-516","url":null,"abstract":"The Mangrove ecosystem is an ecosystem that is around the edge of the coast and many organisms live in this ecosystem, one of them is crabs. The crabs that are often found are deposit crabs from the Ocypodidae and Sesarmidae families that act as balancers in the mangrove ecosystem. This study aimed to analyze species, diversity, evenness, and dominance in the mangrove vegetation area of Koto XI Tarusan District, West Sumatera, Indonesia. The method used is the descriptive method. The biodiversity of brachyuran crabs’ families, Ocypodidae and Sesarmidae, was analyzed with the Shannon-Wiener index (H’). Four indices were calculated for crabs Ocypodidae and Sesarmidae: species identification, Shannon-wiener (H’) diversity, evenness, and dominance indices. The results showed that there were six species of deposited crabs from the Ocypodidae and Sesarmidae families, namely Uca bellator, U. rosea, Perisesarma eumolpe, P. plicatum, Sarmatium germaimi, and Sesarma curoense. The most abundant species (Di) came from the family Sesarmidae. However, for all stations, diversity (H') has a value that is in the medium category, and evenness (E) is in the high category at each station. The dominance (D) obtained a value categorized as low at each observation station. The condition of environmental parameters in the mangrove vegetation area is included in the category of a good environment. It is still within normal limits for the crabs of the Ocypodidae and Sesarmidae families to live. Two substrate types are found in each observation path: muddy and sandy mud.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"73 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140486432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbiota Profile of Infants with Breastfeeding and Mixed Feeding Patterns 母乳喂养和混合喂养婴儿的肠道微生物群特征

HAYATI Journal of Biosciences Pub Date : 2024-01-29 DOI: 10.4308/hjb.31.3.530-538

Tina Kusumaningrum, Wisnu Tafroji, Septiani Madonna Gultom, Nina Dwi Putri, Cut Nurul Hafifah, D. Safari

引用次数: 0

The Study of Exogenous Auxin and Cytokinins in Embryogenesis and Fiber Genes Expression during In Vitro Regeneration of Cotton (Gossypium hirsutum L.) 外源叶黄素和细胞分裂素在棉花（Gossypium hirsutum L.）胚胎发生和体外再生过程中纤维基因表达的研究

HAYATI Journal of Biosciences Pub Date : 2024-01-29 DOI: 10.4308/hjb.31.3.517-529

Mitha Aprilia Mufadilah, Nurhaliza Thamrin, Agung Nugroho Puspito, Mohammad Ubaidillah

{"title":"The Study of Exogenous Auxin and Cytokinins in Embryogenesis and Fiber Genes Expression during In Vitro Regeneration of Cotton (Gossypium hirsutum L.)","authors":"Mitha Aprilia Mufadilah, Nurhaliza Thamrin, Agung Nugroho Puspito, Mohammad Ubaidillah","doi":"10.4308/hjb.31.3.517-529","DOIUrl":"https://doi.org/10.4308/hjb.31.3.517-529","url":null,"abstract":"The efficiency of regeneration media, based on the morpho-cytochemical response and embryogenesis gene expression of widely cultivated cotton in Indonesia (Gossypium hirsutum L.), needs to be studied further. This study's objectives are to identify the effect of 2,4-D and IBA hormones on the morphology and induction of cotton callus; to determine the effect of regeneration media on the morphology, cytochemical, and gene expression of cotton plant during embryogenesis. This study was conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated on the regeneration media, namely M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), and M4 (MS Vitamin + 0, 00 ppm 2,4- D + 0.5 ppm IBA + 0.5 ppm Kinetin). The embryogenesis gene expression was observed. The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability. The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization. Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11. Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"21 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140488678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Begomovirus Infection Patterns in Melons of Indonesian Lowlands: Growth Trends Among Ecosystems and Genotypes 印度尼西亚低地甜瓜的 Begomovirus 感染模式：不同生态系统和基因型的生长趋势

HAYATI Journal of Biosciences Pub Date : 2024-01-26 DOI: 10.4308/hjb.31.3.498-506

Rizko Hadi, S. Hartono, B. Daryono

引用次数: 0

Streptococcus agalactiae Associated with "Dark Body" Disease on Snakeskin Gourami Farmed in the Mekong Delta, Vietnam 与越南湄公河三角洲养殖的蛇皮口红 "黑体 "病有关的无乳链球菌

HAYATI Journal of Biosciences Pub Date : 2024-01-17 DOI: 10.4308/hjb.31.3.486-497

T. Dung, Q. Thi, Nguyen Bao Trung

{"title":"Streptococcus agalactiae Associated with \"Dark Body\" Disease on Snakeskin Gourami Farmed in the Mekong Delta, Vietnam","authors":"T. Dung, Q. Thi, Nguyen Bao Trung","doi":"10.4308/hjb.31.3.486-497","DOIUrl":"https://doi.org/10.4308/hjb.31.3.486-497","url":null,"abstract":"Streptococcosis, due to Streptococcus agalactiae, has become a serious challenge for aquaculture around the world. Therefore, the main purpose of this work was to confirm the etiological agent that caused \"dark body\" disease in snakeskin gourami (Trichogaster pectoralis) cultured in the Mekong Delta, Vietnam. Infected fish displayed clinical signs, including anorexia, lethargic swimming on the water surface, corneal opacity, and hemorrhage in the base of the fin. Remarkably, abnormal black coloration on the body and serious hemorrhage at the base of the pectoral fin, and on the body were found in infected fish. In total, 75 bacterial strains were isolated from different diseased fish farms. Based on morphological and physiological characteristics, the API 20 Strep test, and 16S rRNA sequence analysis, the result illustrated that the bacterial isolates were identified as S. agalactiae. Additionally, antibiotic sensitivity testing revealed that all the S. agalactiae isolates were sensitive to amoxicillin, rifampicin, clarithromycin, erythromycin, doxycycline, cephalexin, novobiocin, and sulfamethoxazole-trimethoprim. Interestingly, S. agalactiae strains were only resistant to gentamycin in this study. Two strains, SRT41 and SRT43, carried out an experimental challenge with concentrations from 104 to 107 CFU/ml in healthy snakeskin gourami, and the LD50 values of the isolates, SRĐT41 and SRĐT43, were determined to be 2.15 × 105 and 3.59 × 103 CFU/ml, respectively, after 7 days. To our knowledge, this is the first report of S. agalactiae derived from intensively cultured snakeskin gourami in the Mekong Delta, Vietnam.","PeriodicalId":508900,"journal":{"name":"HAYATI Journal of Biosciences","volume":"28 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140505307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0