Acta ProtozoologicaPub Date : 2019-07-25DOI: 10.4467/16890027ap.19.007.10838
Jhon Lennon Genovez-Oliveira, S. Cardozo, Á. Oliveira, Viviane M. Lima, Ildemar Ferreira, B. P. Berto
{"title":"Morphological and Molecular Identification of Isospora sepetibensis (Chromista: Miozoa: Eimeriidae) from a New Host, Trichothraupis melanops (Passeriformes: Thraupidae: Tachyphoninae) in South America","authors":"Jhon Lennon Genovez-Oliveira, S. Cardozo, Á. Oliveira, Viviane M. Lima, Ildemar Ferreira, B. P. Berto","doi":"10.4467/16890027ap.19.007.10838","DOIUrl":"https://doi.org/10.4467/16890027ap.19.007.10838","url":null,"abstract":"Isospora sepetibensisBerto, Flausino, Luz, Ferreira and Lopes, 2008 is a protozoan coccidian parasite (Chromista: Miozoa: Coccidiomorphea: Coccidia) that was originally described from Brazilian tanagers Ramphocelus bresilius (Linnaeus, 1766) in the Marambaia Island in the Coast of the State of Rio de Janeiro. In the current work, this species was identified from black-goggled tanagers Trichothraupis melanops (Vieillot, 1818) in the Itatiaia National Park, which is a protected area with a high degree of vulnerability in the interior of the State of Rio de Janeiro, distant in more than 100 km of the type- locality. Its oocysts are sub-spherical to elongate ovoidal, 25.9 ´20.7 µm with smooth, bi-layered wall, ~ 1.3 µm and length/width ratio of 1.1–1.4 (1.26). Micropyle and oocyst residuum absent, but one or two polar granules are present. Sporocysts are ellipsoidal, 16.8 ´10.3 µm, with both Stieda and sub-Stieda bodies. Sporocyst residuum present and sporozoites with refractile body and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from other Neotropical passerines and with an Isospora sp. pseudoparasite of voles of Eurasia. This is the first coccidian parasite from a New World tanager to have a molecular identification of the cox1 gene.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44758450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-07-25DOI: 10.4467/16890027ap.19.003.10834
J. Pauline, A. Achary
{"title":"Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach","authors":"J. Pauline, A. Achary","doi":"10.4467/16890027ap.19.003.10834","DOIUrl":"https://doi.org/10.4467/16890027ap.19.003.10834","url":null,"abstract":"The algal medium was optimized to increase the biomass and lipid production of Chlorococcum oleofaciens. The significant variables were screened and chosen from previously reported algal culture media using Plackett Burman Design (PBD). Optimization of the significant variables were performed using central composite design. The Pareto chart for PBD revealed that the salts such as sodium bicarbonate, sodium nitrate, potassium nitrate and ferrous sulphate had enhanced the biomass and lipid production. The variables and its effect on the responses were further studied by central composite design (CCD). A new medium was formulated based on the response surface methodology. The predicted concentration of NaHCO3, NaNO3, KNO3, MgSO4.7H2O were found to be 6.75 g/L, 0.75 g/L, 1.88 g/L and 0.35 g/L respectively. The actual and the predicted total lipid yield for the optimized media was around 0.74 g/L and 0.78 g/L respectively. The optimal medium has been named as AM medium. Growth and the lipid yields of C. oleofaciens were found higher in AM medium. The specific growth rates of C. oleofaciens in AM and CFTRI media were found to be 0.14 day-1 and 0.19 day-1 respectively. The biomass produced by the optimized AM medium was found to be 2.7 times greater compared to the CFTRI medium. The lipid was extracted and GC-MS was performed which revealed that the fatty acids were predominantly of the class C15:0, C18:0, C16:0 and C12:0. It is concluded that besides lipid content, AM medium increased the cell number leading to the increase in biomass.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42843537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-07-25DOI: 10.4467/16890027ap.19.002.10833
Elideth Pacheco Monteiro, D. Silva, O. Sanches, I. Hamoy, E. Matos
{"title":"Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region","authors":"Elideth Pacheco Monteiro, D. Silva, O. Sanches, I. Hamoy, E. Matos","doi":"10.4467/16890027ap.19.002.10833","DOIUrl":"https://doi.org/10.4467/16890027ap.19.002.10833","url":null,"abstract":"A new species of myxozoan, Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), is described based on specimens extracted from the musculature of the Pacuma toadfish, Batrachoides surinamensis, collected in the municipality of Viseu, in the northern Brazilian state of Para. A total of 60 specimens of B. surinamensis were examined, of which 52 (86%) presented whitish pseudocysts containing numerous rounded spores (7.2±0.2 μm in length and 5.2±0.2 μm in width). These spores have four polar capsules of equal size, measuring 1.8±0.2 μm x 1.3±0.1 μm in the apical view, and 2.7±0.2 μm x 1.3±0.1 μm in the lateral view. A partial sequence (1400 bps) of the small subunit ribosomal RNA gene was obtained and deposited in GenBank (access number: MK256272). The comparison of the morphological and molecular data with those of other Kudoa species supported the description of a new species of mixosporean from the Amazon region, which is denominated here as Kudoa viseuensis n. sp.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43638551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-07-25DOI: 10.4467/16890027ap.19.005.10836
Kang-San Kim, KYU-SEOK Chae, G. Min
{"title":"Morphology and Molecular Analyses of a New Marine Ciliate, Arcuseries minima sp. nov. (Ciliophora: Urostylidae)","authors":"Kang-San Kim, KYU-SEOK Chae, G. Min","doi":"10.4467/16890027ap.19.005.10836","DOIUrl":"https://doi.org/10.4467/16890027ap.19.005.10836","url":null,"abstract":"A new marine urostylid ciliate, Arcuseries minima sp. nov., was discovered in South Korea. Morphological observations and molecular phylogenetic analyses based on small subunit ribosomal DNA (18S rDNA) sequences were used to describe the new species. Arcuseries minima is most similar to A. scutellum among all Arcuseries species, but differs in the following main characters: number of adoral membranelles (13–16 vs. 17 or 18), cortical granules (yellowish, clustered around cirri and dorsal bristles vs. colorless, irregularly scattered), number of macronuclear nodules (20–27 vs. 42–90), number of midventral cirri (5–10 vs. 12–14), and number f transverse cirri (5 or 6 vs. 8). The new species and A. scutellum differ from A. petzi and A. warreni in having smaller body size (≤80 μm vs. ≥80 μm) and fewer cirri: left marginal (≤ 17 vs. ≥ 18) and transverse (≤ 8 vs. ≥8) cirri. This relationship was supported by the phylogenetic tree, where these two groups were separated into two branches.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49668819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-07-25DOI: 10.4467/16890027ap.19.001.10832
Piotr Solarczyk, N. Osten-Sacken, A. Frantz, Simone Schneider, Jacques B. Pir, M. Heddergott
{"title":"First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg","authors":"Piotr Solarczyk, N. Osten-Sacken, A. Frantz, Simone Schneider, Jacques B. Pir, M. Heddergott","doi":"10.4467/16890027ap.19.001.10832","DOIUrl":"https://doi.org/10.4467/16890027ap.19.001.10832","url":null,"abstract":"Giardia duodenalisis one of the most widespread intestinal parasites of humans and other vertebrates. In terms of public health, identification of Giardia assemblages in wildlife is important because only some assemblages of G. duodenalis can infect humans. Here, we use loop-mediated isothermal amplification (LAMP) and genotyping of analysis of the β-giardin geneto screen the zoonotic assemblages of G. duodenalis recovered from faeces of free-living European wildcats (Felis s. silvestris) from Luxembourg. Giardia DNA was detected in one animal (10%) and assigned to assemblage B by both methods. This is the first detection and genotyping of G. duodenalis in a European wild felid in general, and of assemblage B in particular. Free-living wildcats may act as reservoirs of G. duodenalis infectious for humans and other wildlife and domestic animals. Using a combination of LAMP- and genotyping-based methods allowed effective, sensitive, and rapid detection of a zoonotic G. duodenalis assemblage B in wildlife.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45190151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-05-24Epub Date: 2019-01-11DOI: 10.4467/16890027AP.18.015.10091
Michael S Gruber, Alexandra Mühlthaler, Sabine Agatha
{"title":"Ultrastructural Studies on a Model Tintinnid - <i>Schmidingerella meunieri</i> (Kofoid and Campbell, 1929) Agatha and Strüder-Kypke, 2012 (Ciliophora). I. Somatic Kinetids with Unique Ultrastructure.","authors":"Michael S Gruber, Alexandra Mühlthaler, Sabine Agatha","doi":"10.4467/16890027AP.18.015.10091","DOIUrl":"10.4467/16890027AP.18.015.10091","url":null,"abstract":"<p><p>Molecular phylogenies of Oligotrichea currently do not contain all genera and families and display topologies which are often incongruent with morphological findings. In ciliates, the somatic kinetids are rather conserved, i.e., their ultrastructures, particularly the fibrillar associates, often characterise the main groups, except for the choreotrichids. Four different kinetid types are found in protargol-stained choreotrichids and used for reconstructing the taxon's evolution (the \"Kinetid Transformation Hypothesis\"). Proof for this hypothesis requires transmission electron microscopic studies, which are very rare in the choreotrichids and oligotrichids. Such an approach provides insights into the ultrastructural variability of somatic kinetids in spirotrichs and may also detect apomorphies characterising certain choreotrichid families. In the model tintinnid <i>Schmidingerella meunieri</i>, the ultrastructure of the three kinetid types in the somatic ciliature is studied in cryofixed cells. The data support the \"Kinetid Transformation Hypothesis\" regarding tintinnids with a ventral kinety. This first detailed study on kinetids in tintinnids and choreotrichids in general reveals totally new kinetid types in ciliates: beyond the three common associates, they are characterised by two or three conspicuous microtubular ribbons extending on the kinetids' left sides. These extraordinary ribbons form together with the overlapping postciliary ribbons a unique network in the cortex of the anterior cell portion. The evolutionary constrains which might have fostered the development of such structures are discussed for the Oligotrichea, the choreotrichids, and tintinnids as their first occurrence is currently uncertain. Additionally, the kinetids in tintinnids, aloricate choreotrichids, oligotrichids, hypotrichs, and euplotids are compared.</p>","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 3","pages":"195-214"},"PeriodicalIF":1.5,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546599/pdf/EMS83093.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40448904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Redescription of <i>Dexiotricha colpidiopsis</i> (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for <i>Dexiotricha</i> species.","authors":"Zhishuai Qu, René Groben, Viggó Marteinsson, Sabine Agatha, Sabine Filker, Thorsten Stoeck","doi":"10.4467/16890027AP.18.009.8983","DOIUrl":"https://doi.org/10.4467/16890027AP.18.009.8983","url":null,"abstract":"<p><p>We isolated an encysted ciliate from a geothermal field in Iceland. The morphological features of this isolate fit the descriptions of <i>Dexiotricha colpidiopsis</i> Kahl, 1926) Jankowski, 1964 very well. These comprise body shape and size in vivo, the number of somatic kineties, and the positions of macronucleus and contractile vacuole. Using state-of-the-art taxonomic methods, the species is redescribed, including phylogenetic analyses of the small subunit ribosomal RNA (SSU rRNA) gene as molecular marker. In the phylogenetic analyses, <i>D. colpidiopsis</i> clusters with the three available SSU rRNA gene sequences of congeners, suggesting a monophyly of the genus <i>Dexiotricha</i>. Its closest relative in phylogenetic analyses is <i>D. elliptica</i>, which also shows a high morphological similarity. This is the first record of a <i>Dexiotricha</i> species from a hot spring, indicating a wide temperature tolerance of this species at least in the encysted state. The new findings on <i>D. colpidiopsis</i> are included in a briefly revision of the scuticociliate genus <i>Dexiotricha</i> and an identification key to the species.</p>","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 2","pages":"95-106"},"PeriodicalIF":0.5,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546606/pdf/EMS83097.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40450959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-05-24Epub Date: 2019-01-11DOI: 10.4467/16890027AP.18.014.10090
Christian F Bardele, Nicole Stockmann, Sabine Agatha
{"title":"Some Ultrastructural Features of the Planktonic Freshwater Ciliate <i>Limnostrombidium viride</i> (Alveolata, Ciliophora, Oligotrichida) and Improved Diagnoses of Oligotrich Taxa.","authors":"Christian F Bardele, Nicole Stockmann, Sabine Agatha","doi":"10.4467/16890027AP.18.014.10090","DOIUrl":"10.4467/16890027AP.18.014.10090","url":null,"abstract":"<p><p>The first transmission and scanning electron microscopical studies in combination with freeze-fracture technology have disclosed some important morphological and ultrastructural features in the freshwater oligotrichid <i>Limnostrombidium viride</i>. (I) The dikinetids (paired basal bodies) of the girdle kinety have a club-shaped cilium associated only with each left basal body. The electron-dense (paraflagellar) body on one side of its \"9×2+2\"-axoneme and the regular array of intramembranous particles indicate a sensory, perhaps photoreceptor function of these club-shaped cilia. (II) The stichomonad endoral membrane is proximally covered by a cytoplasmic fold and distally by multiple membranous layers. Thus entirely covered, the endoral is probably no longer involved in food capture; nonetheless, its associated microtubules might stabilise the cytopharynx. (III) Instead of a contractile vacuole, a horizontal ring-canal with supposed osmoregulatory function occurs. (IV) The extrusive nature of the trichites is not only observed in electron micrographs, but the attachment sites of these organelles also display a rosette of \"8+1\"-particles in the P-face of freeze-fracture replicas typical for ciliate extrusomes. (V) The neoformation organelle, the subsurface tube in which stomatogenesis takes place, shows short basal bodies and normal axonemes about 1 μm long. It is accompanied by numerous membrane vesicles, which might provide membrane material for the outgrowing cilia.</p>","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 3","pages":"169-193"},"PeriodicalIF":1.5,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546604/pdf/EMS83092.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40539873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-02-12DOI: 10.4467/16890027AP.18.018.10094
K. Prokina, Dmitry G. Zagumyonnyi, D. Tikhonenkov
{"title":"Centrohelid Heliozoans (Centroplasthelida Febvre-Chevalier et Febvre, 1984) from Different Types of Freshwater Bodies in the Middle Russian Forest-steppe","authors":"K. Prokina, Dmitry G. Zagumyonnyi, D. Tikhonenkov","doi":"10.4467/16890027AP.18.018.10094","DOIUrl":"https://doi.org/10.4467/16890027AP.18.018.10094","url":null,"abstract":"Centrohelid Heliozoans (Centroplasthelida Febvre-Chevalier et Febvre, 1984) from Different Types of Freshwater Bodies in the Middle Russian Forest-steppe","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 1","pages":"245-266"},"PeriodicalIF":0.5,"publicationDate":"2019-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43943608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ProtozoologicaPub Date : 2019-02-12DOI: 10.4467/16890027AP.18.016.10092
Hoan Q. Trana, Y. Mazei
{"title":"Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.","authors":"Hoan Q. Trana, Y. Mazei","doi":"10.4467/16890027AP.18.016.10092","DOIUrl":"https://doi.org/10.4467/16890027AP.18.016.10092","url":null,"abstract":"Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"57 1","pages":"215-229"},"PeriodicalIF":0.5,"publicationDate":"2019-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47270867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}